Rapid molecular identification of the harmful freshwater dinoflagellate Peridinium in various life stages using genus-specific single-cell PCR

Rapid molecular identification of the harmful freshwater dinoflagellate Peridinium in various life stages using genus-specific single-cell PCR

Cysts of the freshwater dinoflagellate Peridinium are typically different from vegetative cells in shape and remain largely undescribed. Molecular discrimination of such cysts would be useful to a number of research disciplines. A reliable method for the amplification of ribosomal DNA (rDNA) from Pe...

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Veröffentlicht in:Journal of applied phycology 2007-10, Vol.19 (5), p.467-470
Hauptverfasser: Ki, Jang-Seu, Han, Myung-Soo
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Sprache:eng
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Dinoflagellate cyst
Freshwater
Planozygote
rDNA
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description Cysts of the freshwater dinoflagellate Peridinium are typically different from vegetative cells in shape and remain largely undescribed. Molecular discrimination of such cysts would be useful to a number of research disciplines. A reliable method for the amplification of ribosomal DNA (rDNA) from Peridinium at different life stages is described. This genotyping strategy relies on whole cell PCR using Peridinium-specific primers designed from available 18S rDNA sequences. Here, we demonstrate the effectiveness of Peridinium-specific PCR for the rapid molecular identification of Peridinium cells in various life stages such as vegetative, planozygote, hypnozygote and cyst.
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subjects Dinoflagellate cyst
Freshwater
Planozygote
rDNA
title Rapid molecular identification of the harmful freshwater dinoflagellate Peridinium in various life stages using genus-specific single-cell PCR
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