c-FOS and n-NOS reactive neurons in response to circulating Phoneutria nigriventer spider venom

Abstract Drugs and neurotoxins activate specific neural circuits by increasing or decreasing the formation and release of neurotransmitters, such as nitric oxide (NO), and by inducing immediate early genes, such as FOS. We have previously shown that Phoneutria nigriventer spider venom (PNV) impairs...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Brain research bulletin 2007-06, Vol.73 (1), p.114-126
Hauptverfasser: da Cruz-Höfling, Maria Alice, Zago, Gabriela Mariotoni, Melo, Liana Lins, Rapôso, Catarina
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 126
container_issue 1
container_start_page 114
container_title Brain research bulletin
container_volume 73
creator da Cruz-Höfling, Maria Alice
Zago, Gabriela Mariotoni
Melo, Liana Lins
Rapôso, Catarina
description Abstract Drugs and neurotoxins activate specific neural circuits by increasing or decreasing the formation and release of neurotransmitters, such as nitric oxide (NO), and by inducing immediate early genes, such as FOS. We have previously shown that Phoneutria nigriventer spider venom (PNV) impairs the microtubule-dependent transcellular barrier of the blood–brain interface and causes structural alterations in perivascular astrocytic end-feet without producing morphological changes in central neuronal cells. In the present study, we used FOS and neuronal nitric oxide synthase (n-NOS) immunolabeling to investigate the ability of PNV to activate the central nervous system. Three groups of rats were used: the first group received a sublethal dose of PNV (850 μg/kg, via a tail vein), the second received an equal volume of 0.9% saline (sham group) and the third group received no injection. Envenomed rats showed salivation, lachrymation, tremors and flaccidity followed by spastic paralysis of the hind limbs and convulsions. Cryosections (30 μm thick) were serially collected at 600 μm intervals for free-floating immunohistochemical analysis. FOS-like positive neurons predominated in motor-related areas such as dorsolateral (dlPAG) and ventral periaqueductal gray matter (vPAG), frontal (FCM) and parietal motor cortex (PCM), and periventricular thalamic nucleus (PTN) and in acute stress-related areas (rhinal cortex and lateral septal nuclei). The greatest relative increases in FOS-like positive neurons occurred in the vPAG, PCM and PTN motor-related areas. n-NOS-positive neurons predominated in the periventricular thalamic nuclei, followed by the dorsolateral periaqueductal gray matter and parietal cortex motor area. The marked activation of motor areas and, to a lesser extent, of acute stress-related areas suggested the involvement of neuronal pathways in these regions in the response to envenoming by PNV. In addition, the occurrence of n-NOS immunolabeling in some anatomical regions with FOS-like positive neurons suggests that NO may modulate the response to PNV in these regions.
doi_str_mv 10.1016/j.brainresbull.2007.01.017
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19721051</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0361923007000949</els_id><sourcerecordid>19721051</sourcerecordid><originalsourceid>FETCH-LOGICAL-c464t-dd73e1744683ee1212a4c1627d1673ed0fca7ef03aed3a6981655ea391c32b023</originalsourceid><addsrcrecordid>eNqNUcFqGzEQFaElcdP-QhA59LbujLQreXsIlKRpC6EppD0LWRoncteSK-0G8veVsSGhp8LAzEjvzUjvMXaOMEdA9WE9X2YbYqaynIZhLgD0HLCGPmIzXGjZCN3qV2wGUmHTCwkn7E0pawBQi04dsxPUbd-rtpsx45rr2ztuo-ex-V6rTNaN4ZF4pCmnWHiI9axsa0l8TNyF7KbBjiHe8x8PqaLGHCyP4T5XVhwp87INvqbapc1b9nplh0LvDvmU_br-_PPya3Nz--Xb5aebxrWqHRvvtaT6qlYtJBEKFLZ1qIT2qOqNh5WzmlYgLXlpVb9A1XVkZY9OiiUIecre7-duc_ozURnNJhRHw2AjpakY7LVA6LACP-6BLqdSMq3MNoeNzU8GwezkNWvzUl6zk9cA1tCVfHbYMi035J-pBz0r4GoPoPrXx0DZFBcoOvIhkxuNT-H_9lz8M8YNIQZnh9_0RGWdphyrmgZNEQbM3c7onc-gq8d928u_JAKoQg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19721051</pqid></control><display><type>article</type><title>c-FOS and n-NOS reactive neurons in response to circulating Phoneutria nigriventer spider venom</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><creator>da Cruz-Höfling, Maria Alice ; Zago, Gabriela Mariotoni ; Melo, Liana Lins ; Rapôso, Catarina</creator><creatorcontrib>da Cruz-Höfling, Maria Alice ; Zago, Gabriela Mariotoni ; Melo, Liana Lins ; Rapôso, Catarina</creatorcontrib><description>Abstract Drugs and neurotoxins activate specific neural circuits by increasing or decreasing the formation and release of neurotransmitters, such as nitric oxide (NO), and by inducing immediate early genes, such as FOS. We have previously shown that Phoneutria nigriventer spider venom (PNV) impairs the microtubule-dependent transcellular barrier of the blood–brain interface and causes structural alterations in perivascular astrocytic end-feet without producing morphological changes in central neuronal cells. In the present study, we used FOS and neuronal nitric oxide synthase (n-NOS) immunolabeling to investigate the ability of PNV to activate the central nervous system. Three groups of rats were used: the first group received a sublethal dose of PNV (850 μg/kg, via a tail vein), the second received an equal volume of 0.9% saline (sham group) and the third group received no injection. Envenomed rats showed salivation, lachrymation, tremors and flaccidity followed by spastic paralysis of the hind limbs and convulsions. Cryosections (30 μm thick) were serially collected at 600 μm intervals for free-floating immunohistochemical analysis. FOS-like positive neurons predominated in motor-related areas such as dorsolateral (dlPAG) and ventral periaqueductal gray matter (vPAG), frontal (FCM) and parietal motor cortex (PCM), and periventricular thalamic nucleus (PTN) and in acute stress-related areas (rhinal cortex and lateral septal nuclei). The greatest relative increases in FOS-like positive neurons occurred in the vPAG, PCM and PTN motor-related areas. n-NOS-positive neurons predominated in the periventricular thalamic nuclei, followed by the dorsolateral periaqueductal gray matter and parietal cortex motor area. The marked activation of motor areas and, to a lesser extent, of acute stress-related areas suggested the involvement of neuronal pathways in these regions in the response to envenoming by PNV. In addition, the occurrence of n-NOS immunolabeling in some anatomical regions with FOS-like positive neurons suggests that NO may modulate the response to PNV in these regions.</description><identifier>ISSN: 0361-9230</identifier><identifier>EISSN: 1873-2747</identifier><identifier>DOI: 10.1016/j.brainresbull.2007.01.017</identifier><identifier>PMID: 17499645</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Acute stress-related area ; Amygdala - cytology ; Amygdala - drug effects ; Amygdala - metabolism ; Animals ; Araneae ; Brain ; Brain Chemistry - drug effects ; Cerebral Cortex - cytology ; Cerebral Cortex - drug effects ; Cerebral Cortex - metabolism ; Convulsion ; Data Interpretation, Statistical ; Hippocampus - cytology ; Hippocampus - drug effects ; Hippocampus - metabolism ; Immunohistochemistry ; Injections, Intravenous ; Male ; Motor-related area ; Neurology ; Neuronal activation ; Neurons - enzymology ; Neurons - physiology ; Nitric oxide ; Nitric Oxide Synthase Type I - metabolism ; Periaqueductal Gray - cytology ; Periaqueductal Gray - drug effects ; Periaqueductal Gray - metabolism ; Phoneutria nigriventer ; Proto-Oncogene Proteins c-fos - metabolism ; Rats ; Rats, Wistar ; Spider Venoms - administration &amp; dosage ; Spider Venoms - pharmacology</subject><ispartof>Brain research bulletin, 2007-06, Vol.73 (1), p.114-126</ispartof><rights>Elsevier Inc.</rights><rights>2007 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-dd73e1744683ee1212a4c1627d1673ed0fca7ef03aed3a6981655ea391c32b023</citedby><cites>FETCH-LOGICAL-c464t-dd73e1744683ee1212a4c1627d1673ed0fca7ef03aed3a6981655ea391c32b023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.brainresbull.2007.01.017$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17499645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>da Cruz-Höfling, Maria Alice</creatorcontrib><creatorcontrib>Zago, Gabriela Mariotoni</creatorcontrib><creatorcontrib>Melo, Liana Lins</creatorcontrib><creatorcontrib>Rapôso, Catarina</creatorcontrib><title>c-FOS and n-NOS reactive neurons in response to circulating Phoneutria nigriventer spider venom</title><title>Brain research bulletin</title><addtitle>Brain Res Bull</addtitle><description>Abstract Drugs and neurotoxins activate specific neural circuits by increasing or decreasing the formation and release of neurotransmitters, such as nitric oxide (NO), and by inducing immediate early genes, such as FOS. We have previously shown that Phoneutria nigriventer spider venom (PNV) impairs the microtubule-dependent transcellular barrier of the blood–brain interface and causes structural alterations in perivascular astrocytic end-feet without producing morphological changes in central neuronal cells. In the present study, we used FOS and neuronal nitric oxide synthase (n-NOS) immunolabeling to investigate the ability of PNV to activate the central nervous system. Three groups of rats were used: the first group received a sublethal dose of PNV (850 μg/kg, via a tail vein), the second received an equal volume of 0.9% saline (sham group) and the third group received no injection. Envenomed rats showed salivation, lachrymation, tremors and flaccidity followed by spastic paralysis of the hind limbs and convulsions. Cryosections (30 μm thick) were serially collected at 600 μm intervals for free-floating immunohistochemical analysis. FOS-like positive neurons predominated in motor-related areas such as dorsolateral (dlPAG) and ventral periaqueductal gray matter (vPAG), frontal (FCM) and parietal motor cortex (PCM), and periventricular thalamic nucleus (PTN) and in acute stress-related areas (rhinal cortex and lateral septal nuclei). The greatest relative increases in FOS-like positive neurons occurred in the vPAG, PCM and PTN motor-related areas. n-NOS-positive neurons predominated in the periventricular thalamic nuclei, followed by the dorsolateral periaqueductal gray matter and parietal cortex motor area. The marked activation of motor areas and, to a lesser extent, of acute stress-related areas suggested the involvement of neuronal pathways in these regions in the response to envenoming by PNV. In addition, the occurrence of n-NOS immunolabeling in some anatomical regions with FOS-like positive neurons suggests that NO may modulate the response to PNV in these regions.</description><subject>Acute stress-related area</subject><subject>Amygdala - cytology</subject><subject>Amygdala - drug effects</subject><subject>Amygdala - metabolism</subject><subject>Animals</subject><subject>Araneae</subject><subject>Brain</subject><subject>Brain Chemistry - drug effects</subject><subject>Cerebral Cortex - cytology</subject><subject>Cerebral Cortex - drug effects</subject><subject>Cerebral Cortex - metabolism</subject><subject>Convulsion</subject><subject>Data Interpretation, Statistical</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Immunohistochemistry</subject><subject>Injections, Intravenous</subject><subject>Male</subject><subject>Motor-related area</subject><subject>Neurology</subject><subject>Neuronal activation</subject><subject>Neurons - enzymology</subject><subject>Neurons - physiology</subject><subject>Nitric oxide</subject><subject>Nitric Oxide Synthase Type I - metabolism</subject><subject>Periaqueductal Gray - cytology</subject><subject>Periaqueductal Gray - drug effects</subject><subject>Periaqueductal Gray - metabolism</subject><subject>Phoneutria nigriventer</subject><subject>Proto-Oncogene Proteins c-fos - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Spider Venoms - administration &amp; dosage</subject><subject>Spider Venoms - pharmacology</subject><issn>0361-9230</issn><issn>1873-2747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUcFqGzEQFaElcdP-QhA59LbujLQreXsIlKRpC6EppD0LWRoncteSK-0G8veVsSGhp8LAzEjvzUjvMXaOMEdA9WE9X2YbYqaynIZhLgD0HLCGPmIzXGjZCN3qV2wGUmHTCwkn7E0pawBQi04dsxPUbd-rtpsx45rr2ztuo-ex-V6rTNaN4ZF4pCmnWHiI9axsa0l8TNyF7KbBjiHe8x8PqaLGHCyP4T5XVhwp87INvqbapc1b9nplh0LvDvmU_br-_PPya3Nz--Xb5aebxrWqHRvvtaT6qlYtJBEKFLZ1qIT2qOqNh5WzmlYgLXlpVb9A1XVkZY9OiiUIecre7-duc_ozURnNJhRHw2AjpakY7LVA6LACP-6BLqdSMq3MNoeNzU8GwezkNWvzUl6zk9cA1tCVfHbYMi035J-pBz0r4GoPoPrXx0DZFBcoOvIhkxuNT-H_9lz8M8YNIQZnh9_0RGWdphyrmgZNEQbM3c7onc-gq8d928u_JAKoQg</recordid><startdate>20070615</startdate><enddate>20070615</enddate><creator>da Cruz-Höfling, Maria Alice</creator><creator>Zago, Gabriela Mariotoni</creator><creator>Melo, Liana Lins</creator><creator>Rapôso, Catarina</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope></search><sort><creationdate>20070615</creationdate><title>c-FOS and n-NOS reactive neurons in response to circulating Phoneutria nigriventer spider venom</title><author>da Cruz-Höfling, Maria Alice ; Zago, Gabriela Mariotoni ; Melo, Liana Lins ; Rapôso, Catarina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c464t-dd73e1744683ee1212a4c1627d1673ed0fca7ef03aed3a6981655ea391c32b023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acute stress-related area</topic><topic>Amygdala - cytology</topic><topic>Amygdala - drug effects</topic><topic>Amygdala - metabolism</topic><topic>Animals</topic><topic>Araneae</topic><topic>Brain</topic><topic>Brain Chemistry - drug effects</topic><topic>Cerebral Cortex - cytology</topic><topic>Cerebral Cortex - drug effects</topic><topic>Cerebral Cortex - metabolism</topic><topic>Convulsion</topic><topic>Data Interpretation, Statistical</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Immunohistochemistry</topic><topic>Injections, Intravenous</topic><topic>Male</topic><topic>Motor-related area</topic><topic>Neurology</topic><topic>Neuronal activation</topic><topic>Neurons - enzymology</topic><topic>Neurons - physiology</topic><topic>Nitric oxide</topic><topic>Nitric Oxide Synthase Type I - metabolism</topic><topic>Periaqueductal Gray - cytology</topic><topic>Periaqueductal Gray - drug effects</topic><topic>Periaqueductal Gray - metabolism</topic><topic>Phoneutria nigriventer</topic><topic>Proto-Oncogene Proteins c-fos - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Spider Venoms - administration &amp; dosage</topic><topic>Spider Venoms - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>da Cruz-Höfling, Maria Alice</creatorcontrib><creatorcontrib>Zago, Gabriela Mariotoni</creatorcontrib><creatorcontrib>Melo, Liana Lins</creatorcontrib><creatorcontrib>Rapôso, Catarina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><jtitle>Brain research bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>da Cruz-Höfling, Maria Alice</au><au>Zago, Gabriela Mariotoni</au><au>Melo, Liana Lins</au><au>Rapôso, Catarina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>c-FOS and n-NOS reactive neurons in response to circulating Phoneutria nigriventer spider venom</atitle><jtitle>Brain research bulletin</jtitle><addtitle>Brain Res Bull</addtitle><date>2007-06-15</date><risdate>2007</risdate><volume>73</volume><issue>1</issue><spage>114</spage><epage>126</epage><pages>114-126</pages><issn>0361-9230</issn><eissn>1873-2747</eissn><abstract>Abstract Drugs and neurotoxins activate specific neural circuits by increasing or decreasing the formation and release of neurotransmitters, such as nitric oxide (NO), and by inducing immediate early genes, such as FOS. We have previously shown that Phoneutria nigriventer spider venom (PNV) impairs the microtubule-dependent transcellular barrier of the blood–brain interface and causes structural alterations in perivascular astrocytic end-feet without producing morphological changes in central neuronal cells. In the present study, we used FOS and neuronal nitric oxide synthase (n-NOS) immunolabeling to investigate the ability of PNV to activate the central nervous system. Three groups of rats were used: the first group received a sublethal dose of PNV (850 μg/kg, via a tail vein), the second received an equal volume of 0.9% saline (sham group) and the third group received no injection. Envenomed rats showed salivation, lachrymation, tremors and flaccidity followed by spastic paralysis of the hind limbs and convulsions. Cryosections (30 μm thick) were serially collected at 600 μm intervals for free-floating immunohistochemical analysis. FOS-like positive neurons predominated in motor-related areas such as dorsolateral (dlPAG) and ventral periaqueductal gray matter (vPAG), frontal (FCM) and parietal motor cortex (PCM), and periventricular thalamic nucleus (PTN) and in acute stress-related areas (rhinal cortex and lateral septal nuclei). The greatest relative increases in FOS-like positive neurons occurred in the vPAG, PCM and PTN motor-related areas. n-NOS-positive neurons predominated in the periventricular thalamic nuclei, followed by the dorsolateral periaqueductal gray matter and parietal cortex motor area. The marked activation of motor areas and, to a lesser extent, of acute stress-related areas suggested the involvement of neuronal pathways in these regions in the response to envenoming by PNV. In addition, the occurrence of n-NOS immunolabeling in some anatomical regions with FOS-like positive neurons suggests that NO may modulate the response to PNV in these regions.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>17499645</pmid><doi>10.1016/j.brainresbull.2007.01.017</doi><tpages>13</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0361-9230
ispartof Brain research bulletin, 2007-06, Vol.73 (1), p.114-126
issn 0361-9230
1873-2747
language eng
recordid cdi_proquest_miscellaneous_19721051
source MEDLINE; Elsevier ScienceDirect Journals Complete
subjects Acute stress-related area
Amygdala - cytology
Amygdala - drug effects
Amygdala - metabolism
Animals
Araneae
Brain
Brain Chemistry - drug effects
Cerebral Cortex - cytology
Cerebral Cortex - drug effects
Cerebral Cortex - metabolism
Convulsion
Data Interpretation, Statistical
Hippocampus - cytology
Hippocampus - drug effects
Hippocampus - metabolism
Immunohistochemistry
Injections, Intravenous
Male
Motor-related area
Neurology
Neuronal activation
Neurons - enzymology
Neurons - physiology
Nitric oxide
Nitric Oxide Synthase Type I - metabolism
Periaqueductal Gray - cytology
Periaqueductal Gray - drug effects
Periaqueductal Gray - metabolism
Phoneutria nigriventer
Proto-Oncogene Proteins c-fos - metabolism
Rats
Rats, Wistar
Spider Venoms - administration & dosage
Spider Venoms - pharmacology
title c-FOS and n-NOS reactive neurons in response to circulating Phoneutria nigriventer spider venom
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T14%3A03%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=c-FOS%20and%20n-NOS%20reactive%20neurons%20in%20response%20to%20circulating%20Phoneutria%20nigriventer%20spider%20venom&rft.jtitle=Brain%20research%20bulletin&rft.au=da%20Cruz-H%C3%B6fling,%20Maria%20Alice&rft.date=2007-06-15&rft.volume=73&rft.issue=1&rft.spage=114&rft.epage=126&rft.pages=114-126&rft.issn=0361-9230&rft.eissn=1873-2747&rft_id=info:doi/10.1016/j.brainresbull.2007.01.017&rft_dat=%3Cproquest_cross%3E19721051%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19721051&rft_id=info:pmid/17499645&rft_els_id=1_s2_0_S0361923007000949&rfr_iscdi=true