Norcantharidin induce apoptosis in human nasopharyngeal carcinoma through caspase and mitochondrial pathway
While Nasopharyngeal carcinoma (NPC) is uncommon in western countries, it is endemic in Southeast Asia and Southern China. Previous study of norcantharidin (NCTD), isolated from blister beetles, has proved its anticancer effect on various tumors. However, the effect of NCTD in NPC has never been stu...
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| Veröffentlicht in: | Environmental toxicology 2018-03, Vol.33 (3), p.343-350 |
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| creator | Chen, Andy Wei‐Ge Tseng, Yen‐Shuo Lin, Chia‐Chieh Hsi, Yi‐Ting Lo, Yu‐Sheng Chuang, Yi‐Ching Lin, Shu‐Hui Yu, Chia‐Yun Hsieh, Ming‐Ju Chen, Mu‐Kuan |
| description | While Nasopharyngeal carcinoma (NPC) is uncommon in western countries, it is endemic in Southeast Asia and Southern China. Previous study of norcantharidin (NCTD), isolated from blister beetles, has proved its anticancer effect on various tumors. However, the effect of NCTD in NPC has never been studied. The purpose of this study is to inspect the suppression activity of NCTD on NPC, along with the underlying mechanism. NPC cell line NPC‐BM was treated with NCTD. NCTD remarkably inhibited proliferation and induce apoptosis in NPC‐BM cell. Activation of caspase‐3, −8, −9 was observed through western blotting. The expression of antiapoptotic protein Bcl‐XL was significantly reduced, but expression of proapoptotic protein Bak was increased after treatment of NCTD. The cytotoxic effect of NCTD on NPC‐BM cell is mainly due to apoptosis, mediated by caspase and mitochondrial pathway. These results suggested that NCTD could be a potential anticancer agent for NPC. |
| doi_str_mv | 10.1002/tox.22521 |
| format | Article |
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Previous study of norcantharidin (NCTD), isolated from blister beetles, has proved its anticancer effect on various tumors. However, the effect of NCTD in NPC has never been studied. The purpose of this study is to inspect the suppression activity of NCTD on NPC, along with the underlying mechanism. NPC cell line NPC‐BM was treated with NCTD. NCTD remarkably inhibited proliferation and induce apoptosis in NPC‐BM cell. Activation of caspase‐3, −8, −9 was observed through western blotting. The expression of antiapoptotic protein Bcl‐XL was significantly reduced, but expression of proapoptotic protein Bak was increased after treatment of NCTD. The cytotoxic effect of NCTD on NPC‐BM cell is mainly due to apoptosis, mediated by caspase and mitochondrial pathway. These results suggested that NCTD could be a potential anticancer agent for NPC.</description><identifier>ISSN: 1520-4081</identifier><identifier>EISSN: 1522-7278</identifier><identifier>DOI: 10.1002/tox.22521</identifier><identifier>PMID: 29193574</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Anticancer properties ; Antineoplastic Agents - pharmacology ; Apoptosis ; Apoptosis - drug effects ; BAK protein ; Bcl-x protein ; bcl-X Protein - metabolism ; Beetles ; Blistering ; Bridged Bicyclo Compounds, Heterocyclic - pharmacology ; Cancer ; Carcinoma ; Caspase ; Caspase 3 - metabolism ; Caspase 8 - metabolism ; Caspase 9 - metabolism ; Caspase-3 ; Cell activation ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cytotoxicity ; Enzyme Activation ; Humans ; Mitochondria ; Mitochondria - metabolism ; mitochondrial ; Nasopharyngeal Carcinoma ; Nasopharyngeal Neoplasms ; Norcantharidin ; Throat cancer ; Tumors ; Western blotting</subject><ispartof>Environmental toxicology, 2018-03, Vol.33 (3), p.343-350</ispartof><rights>2017 Wiley Periodicals, Inc.</rights><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4561-8a241b5cd3139ff959f09da478c53223fb79c13023a78bc3ed0b25c4ada9c7e43</citedby><cites>FETCH-LOGICAL-c4561-8a241b5cd3139ff959f09da478c53223fb79c13023a78bc3ed0b25c4ada9c7e43</cites><orcidid>0000-0001-9726-3234</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Ftox.22521$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Ftox.22521$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29193574$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Andy Wei‐Ge</creatorcontrib><creatorcontrib>Tseng, Yen‐Shuo</creatorcontrib><creatorcontrib>Lin, Chia‐Chieh</creatorcontrib><creatorcontrib>Hsi, Yi‐Ting</creatorcontrib><creatorcontrib>Lo, Yu‐Sheng</creatorcontrib><creatorcontrib>Chuang, Yi‐Ching</creatorcontrib><creatorcontrib>Lin, Shu‐Hui</creatorcontrib><creatorcontrib>Yu, Chia‐Yun</creatorcontrib><creatorcontrib>Hsieh, Ming‐Ju</creatorcontrib><creatorcontrib>Chen, Mu‐Kuan</creatorcontrib><title>Norcantharidin induce apoptosis in human nasopharyngeal carcinoma through caspase and mitochondrial pathway</title><title>Environmental toxicology</title><addtitle>Environ Toxicol</addtitle><description>While Nasopharyngeal carcinoma (NPC) is uncommon in western countries, it is endemic in Southeast Asia and Southern China. Previous study of norcantharidin (NCTD), isolated from blister beetles, has proved its anticancer effect on various tumors. However, the effect of NCTD in NPC has never been studied. The purpose of this study is to inspect the suppression activity of NCTD on NPC, along with the underlying mechanism. NPC cell line NPC‐BM was treated with NCTD. NCTD remarkably inhibited proliferation and induce apoptosis in NPC‐BM cell. Activation of caspase‐3, −8, −9 was observed through western blotting. The expression of antiapoptotic protein Bcl‐XL was significantly reduced, but expression of proapoptotic protein Bak was increased after treatment of NCTD. The cytotoxic effect of NCTD on NPC‐BM cell is mainly due to apoptosis, mediated by caspase and mitochondrial pathway. These results suggested that NCTD could be a potential anticancer agent for NPC.</description><subject>Anticancer properties</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>BAK protein</subject><subject>Bcl-x protein</subject><subject>bcl-X Protein - metabolism</subject><subject>Beetles</subject><subject>Blistering</subject><subject>Bridged Bicyclo Compounds, Heterocyclic - pharmacology</subject><subject>Cancer</subject><subject>Carcinoma</subject><subject>Caspase</subject><subject>Caspase 3 - metabolism</subject><subject>Caspase 8 - metabolism</subject><subject>Caspase 9 - metabolism</subject><subject>Caspase-3</subject><subject>Cell activation</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cytotoxicity</subject><subject>Enzyme Activation</subject><subject>Humans</subject><subject>Mitochondria</subject><subject>Mitochondria - metabolism</subject><subject>mitochondrial</subject><subject>Nasopharyngeal Carcinoma</subject><subject>Nasopharyngeal Neoplasms</subject><subject>Norcantharidin</subject><subject>Throat cancer</subject><subject>Tumors</subject><subject>Western blotting</subject><issn>1520-4081</issn><issn>1522-7278</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U1P3DAQBmCrKioL7aF_AEXqpRyy6891fESoLUgILiBxiya2Q0wTO9iJYP89hl04IOGLrdHjV6MZhH4SvCQY09UUnpaUCkq-oAURlJaSyurr6xuXHFdkHx2kdI8xVmux_ob2qSKKCckX6P9liBr81EF0xvnCeTNrW8AYxikkl3Kh6OYBfOEhhTGzjb-z0BcaonY-DFBMXQzzXZcraYSU_3pTDG4KugveRJftCFP3CJvvaK-FPtkfu_sQ3fz9c316Vl5c_Ts_PbkoNRdrUlZAOWmENoww1bZKqBYrA1xWWjBKWdtIpQnDlIGsGs2swQ0VmoMBpaXl7BD93uaOMTzMNk314JK2fQ_ehjnVREmy5lXFWKa_PtD7MEefu8sqH06ElFkdb5WOIaVo23qMbsijqAmuXzZQ5w3UrxvI9miXODeDNe_ybeQZrLbg0fV283lSfX11u418Bkhskbk</recordid><startdate>201803</startdate><enddate>201803</enddate><creator>Chen, Andy Wei‐Ge</creator><creator>Tseng, Yen‐Shuo</creator><creator>Lin, Chia‐Chieh</creator><creator>Hsi, Yi‐Ting</creator><creator>Lo, Yu‐Sheng</creator><creator>Chuang, Yi‐Ching</creator><creator>Lin, Shu‐Hui</creator><creator>Yu, Chia‐Yun</creator><creator>Hsieh, Ming‐Ju</creator><creator>Chen, Mu‐Kuan</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7ST</scope><scope>7TN</scope><scope>7U7</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M7N</scope><scope>SOI</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9726-3234</orcidid></search><sort><creationdate>201803</creationdate><title>Norcantharidin induce apoptosis in human nasopharyngeal carcinoma through caspase and mitochondrial pathway</title><author>Chen, Andy Wei‐Ge ; Tseng, Yen‐Shuo ; Lin, Chia‐Chieh ; Hsi, Yi‐Ting ; Lo, Yu‐Sheng ; Chuang, Yi‐Ching ; Lin, Shu‐Hui ; Yu, Chia‐Yun ; Hsieh, Ming‐Ju ; Chen, Mu‐Kuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4561-8a241b5cd3139ff959f09da478c53223fb79c13023a78bc3ed0b25c4ada9c7e43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Anticancer properties</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>BAK protein</topic><topic>Bcl-x protein</topic><topic>bcl-X Protein - metabolism</topic><topic>Beetles</topic><topic>Blistering</topic><topic>Bridged Bicyclo Compounds, Heterocyclic - pharmacology</topic><topic>Cancer</topic><topic>Carcinoma</topic><topic>Caspase</topic><topic>Caspase 3 - metabolism</topic><topic>Caspase 8 - metabolism</topic><topic>Caspase 9 - metabolism</topic><topic>Caspase-3</topic><topic>Cell activation</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cytotoxicity</topic><topic>Enzyme Activation</topic><topic>Humans</topic><topic>Mitochondria</topic><topic>Mitochondria - metabolism</topic><topic>mitochondrial</topic><topic>Nasopharyngeal Carcinoma</topic><topic>Nasopharyngeal Neoplasms</topic><topic>Norcantharidin</topic><topic>Throat cancer</topic><topic>Tumors</topic><topic>Western blotting</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Andy Wei‐Ge</creatorcontrib><creatorcontrib>Tseng, Yen‐Shuo</creatorcontrib><creatorcontrib>Lin, Chia‐Chieh</creatorcontrib><creatorcontrib>Hsi, Yi‐Ting</creatorcontrib><creatorcontrib>Lo, Yu‐Sheng</creatorcontrib><creatorcontrib>Chuang, Yi‐Ching</creatorcontrib><creatorcontrib>Lin, Shu‐Hui</creatorcontrib><creatorcontrib>Yu, Chia‐Yun</creatorcontrib><creatorcontrib>Hsieh, Ming‐Ju</creatorcontrib><creatorcontrib>Chen, Mu‐Kuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Environment Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Environmental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Andy Wei‐Ge</au><au>Tseng, Yen‐Shuo</au><au>Lin, Chia‐Chieh</au><au>Hsi, Yi‐Ting</au><au>Lo, Yu‐Sheng</au><au>Chuang, Yi‐Ching</au><au>Lin, Shu‐Hui</au><au>Yu, Chia‐Yun</au><au>Hsieh, Ming‐Ju</au><au>Chen, Mu‐Kuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Norcantharidin induce apoptosis in human nasopharyngeal carcinoma through caspase and mitochondrial pathway</atitle><jtitle>Environmental toxicology</jtitle><addtitle>Environ Toxicol</addtitle><date>2018-03</date><risdate>2018</risdate><volume>33</volume><issue>3</issue><spage>343</spage><epage>350</epage><pages>343-350</pages><issn>1520-4081</issn><eissn>1522-7278</eissn><abstract>While Nasopharyngeal carcinoma (NPC) is uncommon in western countries, it is endemic in Southeast Asia and Southern China. Previous study of norcantharidin (NCTD), isolated from blister beetles, has proved its anticancer effect on various tumors. However, the effect of NCTD in NPC has never been studied. The purpose of this study is to inspect the suppression activity of NCTD on NPC, along with the underlying mechanism. NPC cell line NPC‐BM was treated with NCTD. NCTD remarkably inhibited proliferation and induce apoptosis in NPC‐BM cell. Activation of caspase‐3, −8, −9 was observed through western blotting. The expression of antiapoptotic protein Bcl‐XL was significantly reduced, but expression of proapoptotic protein Bak was increased after treatment of NCTD. The cytotoxic effect of NCTD on NPC‐BM cell is mainly due to apoptosis, mediated by caspase and mitochondrial pathway. These results suggested that NCTD could be a potential anticancer agent for NPC.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>29193574</pmid><doi>10.1002/tox.22521</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9726-3234</orcidid></addata></record> |
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| ispartof | Environmental toxicology, 2018-03, Vol.33 (3), p.343-350 |
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| subjects | Anticancer properties Antineoplastic Agents - pharmacology Apoptosis Apoptosis - drug effects BAK protein Bcl-x protein bcl-X Protein - metabolism Beetles Blistering Bridged Bicyclo Compounds, Heterocyclic - pharmacology Cancer Carcinoma Caspase Caspase 3 - metabolism Caspase 8 - metabolism Caspase 9 - metabolism Caspase-3 Cell activation Cell Line, Tumor Cell Proliferation - drug effects Cytotoxicity Enzyme Activation Humans Mitochondria Mitochondria - metabolism mitochondrial Nasopharyngeal Carcinoma Nasopharyngeal Neoplasms Norcantharidin Throat cancer Tumors Western blotting |
| title | Norcantharidin induce apoptosis in human nasopharyngeal carcinoma through caspase and mitochondrial pathway |
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