Analysis of tetracycline resistance tet(W) genes and their flanking sequences in intestinal Bifidobacterium species
Objectives The tet(W) gene provides tetracycline resistance to a wide range of anaerobic intestinal and ruminal bacteria, but little is known about the molecular organization of the tet(W) gene. The aim of this study was to gain new insights into the molecular organization of the tet(W) gene in bifi...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2008-10, Vol.62 (4), p.688-693 |
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| creator | Ammor, Mohammed Salim Flórez, Ana Belén Álvarez-Martín, Pablo Margolles, Abelardo Mayo, Baltasar |
| description | Objectives The tet(W) gene provides tetracycline resistance to a wide range of anaerobic intestinal and ruminal bacteria, but little is known about the molecular organization of the tet(W) gene. The aim of this study was to gain new insights into the molecular organization of the tet(W) gene in bifidobacteria strains from humans. Methods A segment of DNA encompassing the whole tet(W) gene and its immediate upstream and downstream sequences was analysed in 10 representative strains of four Bifidobacterium species, of which two have been shown to be tetracycline-susceptible. The non-conserved flanking regions of the tet(W) gene were further analysed in six strains. Results All 10 strains share a core DNA domain of 2154 bp [starting 250 bp upstream of the tet(W) gene start codon and ending 13 bp before the stop codon] with 98% to 100% DNA identity. Except for Bifidobacterium animalis E43, all other strains further share 408 bp upstream and 70 bp downstream of the tet(W) gene. An insertion-like element of 736 bp was found to interrupt the tet(W) coding sequence in Bifidobacterium longum M21, which may be the reason for its tetracycline susceptibility. However, genetic events explaining the susceptible phenotype of B. longum LMG 13197T were not observed. Conclusions The tet(W) genes from all 10 strains shared 98% to 100% DNA and amino acid identity, though large variation was found in their flanking regions. |
| doi_str_mv | 10.1093/jac/dkn280 |
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The aim of this study was to gain new insights into the molecular organization of the tet(W) gene in bifidobacteria strains from humans. Methods A segment of DNA encompassing the whole tet(W) gene and its immediate upstream and downstream sequences was analysed in 10 representative strains of four Bifidobacterium species, of which two have been shown to be tetracycline-susceptible. The non-conserved flanking regions of the tet(W) gene were further analysed in six strains. Results All 10 strains share a core DNA domain of 2154 bp [starting 250 bp upstream of the tet(W) gene start codon and ending 13 bp before the stop codon] with 98% to 100% DNA identity. Except for Bifidobacterium animalis E43, all other strains further share 408 bp upstream and 70 bp downstream of the tet(W) gene. An insertion-like element of 736 bp was found to interrupt the tet(W) coding sequence in Bifidobacterium longum M21, which may be the reason for its tetracycline susceptibility. However, genetic events explaining the susceptible phenotype of B. longum LMG 13197T were not observed. Conclusions The tet(W) genes from all 10 strains shared 98% to 100% DNA and amino acid identity, though large variation was found in their flanking regions.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkn280</identifier><identifier>PMID: 18614524</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Anti-Bacterial Agents - pharmacology ; antibiotic resistance ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacteria ; Bacterial Proteins - genetics ; bifidobacteria ; Bifidobacterium - genetics ; Bifidobacterium - isolation & purification ; Bifidobacterium animalis ; Bifidobacterium longum ; Biological and medical sciences ; Deoxyribonucleic acid ; DNA ; DNA Transposable Elements ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Drug resistance ; Gastrointestinal Tract - microbiology ; Genetics ; Humans ; Medical sciences ; Molecular biology ; Molecular Sequence Data ; Mutagenesis, Insertional ; Pharmacology. Drug treatments ; probiotics ; Recombination, Genetic ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; Sequence Homology, Nucleic Acid ; Tetracycline - pharmacology ; Tetracycline Resistance</subject><ispartof>Journal of antimicrobial chemotherapy, 2008-10, Vol.62 (4), p.688-693</ispartof><rights>The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org 2008</rights><rights>2008 INIST-CNRS</rights><rights>The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-95bcd3123b61ea554337642a500911f6a8fccfc5da2a3373ec32c17ed050a6c73</citedby><cites>FETCH-LOGICAL-c477t-95bcd3123b61ea554337642a500911f6a8fccfc5da2a3373ec32c17ed050a6c73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20702852$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18614524$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ammor, Mohammed Salim</creatorcontrib><creatorcontrib>Flórez, Ana Belén</creatorcontrib><creatorcontrib>Álvarez-Martín, Pablo</creatorcontrib><creatorcontrib>Margolles, Abelardo</creatorcontrib><creatorcontrib>Mayo, Baltasar</creatorcontrib><title>Analysis of tetracycline resistance tet(W) genes and their flanking sequences in intestinal Bifidobacterium species</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Objectives The tet(W) gene provides tetracycline resistance to a wide range of anaerobic intestinal and ruminal bacteria, but little is known about the molecular organization of the tet(W) gene. The aim of this study was to gain new insights into the molecular organization of the tet(W) gene in bifidobacteria strains from humans. Methods A segment of DNA encompassing the whole tet(W) gene and its immediate upstream and downstream sequences was analysed in 10 representative strains of four Bifidobacterium species, of which two have been shown to be tetracycline-susceptible. The non-conserved flanking regions of the tet(W) gene were further analysed in six strains. Results All 10 strains share a core DNA domain of 2154 bp [starting 250 bp upstream of the tet(W) gene start codon and ending 13 bp before the stop codon] with 98% to 100% DNA identity. Except for Bifidobacterium animalis E43, all other strains further share 408 bp upstream and 70 bp downstream of the tet(W) gene. An insertion-like element of 736 bp was found to interrupt the tet(W) coding sequence in Bifidobacterium longum M21, which may be the reason for its tetracycline susceptibility. However, genetic events explaining the susceptible phenotype of B. longum LMG 13197T were not observed. Conclusions The tet(W) genes from all 10 strains shared 98% to 100% DNA and amino acid identity, though large variation was found in their flanking regions.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotic resistance</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>bifidobacteria</subject><subject>Bifidobacterium - genetics</subject><subject>Bifidobacterium - isolation & purification</subject><subject>Bifidobacterium animalis</subject><subject>Bifidobacterium longum</subject><subject>Biological and medical sciences</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Drug resistance</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>Genetics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Molecular biology</subject><subject>Molecular Sequence Data</subject><subject>Mutagenesis, Insertional</subject><subject>Pharmacology. Drug treatments</subject><subject>probiotics</subject><subject>Recombination, Genetic</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology, Amino Acid</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Tetracycline - pharmacology</subject><subject>Tetracycline Resistance</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90VtrFDEUAOAgit1WX_wBEgRFhbG5TJLZx1qqKxaEWlF8CdnMSc3ubGbNyYD7703ZpQUfhEDg5OPkXAh5xtk7zubydOX8ab9OomMPyIy3mjWCzflDMmOSqca0Sh6RY8QVY0wr3T0mR7zTvFWinRE8S27YYUQ6BlqgZOd3fogJaIYaLS55uI2__v6G3kACpC71tPyCmGkYXFrHdEMRfk9QIdKY6imAJda09H0MsR-XzhfIcdpQ3IKPgE_Io-AGhKeH-4R8-3Bxfb5oLr98_HR-dtn41pjSzNXS95ILudQcnFKtlEa3wilWu-NBuy54H7zqnXD1SYKXwnMDPVPMaW_kCXm1z7vNYy0Qi91E9DDUsmGc0PK54ZpxVuGLf-BqnHLtAK3gRmthpKzo7R75PCJmCHab48blneXM3u7B1j3Y_R4qfn7IOC030N_Tw-AreHkADr0bQq6DjnjnBDNMdErcu3Ha_v_DZu_qzuDPnXR5bbWRRtnFj5_2M79aLK7Nlf0q_wLQma3B</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Ammor, Mohammed Salim</creator><creator>Flórez, Ana Belén</creator><creator>Álvarez-Martín, Pablo</creator><creator>Margolles, Abelardo</creator><creator>Mayo, Baltasar</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20081001</creationdate><title>Analysis of tetracycline resistance tet(W) genes and their flanking sequences in intestinal Bifidobacterium species</title><author>Ammor, Mohammed Salim ; Flórez, Ana Belén ; Álvarez-Martín, Pablo ; Margolles, Abelardo ; Mayo, Baltasar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-95bcd3123b61ea554337642a500911f6a8fccfc5da2a3373ec32c17ed050a6c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiotic resistance</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>bifidobacteria</topic><topic>Bifidobacterium - genetics</topic><topic>Bifidobacterium - isolation & purification</topic><topic>Bifidobacterium animalis</topic><topic>Bifidobacterium longum</topic><topic>Biological and medical sciences</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Drug resistance</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>Genetics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Molecular biology</topic><topic>Molecular Sequence Data</topic><topic>Mutagenesis, Insertional</topic><topic>Pharmacology. Drug treatments</topic><topic>probiotics</topic><topic>Recombination, Genetic</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology, Amino Acid</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Tetracycline - pharmacology</topic><topic>Tetracycline Resistance</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ammor, Mohammed Salim</creatorcontrib><creatorcontrib>Flórez, Ana Belén</creatorcontrib><creatorcontrib>Álvarez-Martín, Pablo</creatorcontrib><creatorcontrib>Margolles, Abelardo</creatorcontrib><creatorcontrib>Mayo, Baltasar</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ammor, Mohammed Salim</au><au>Flórez, Ana Belén</au><au>Álvarez-Martín, Pablo</au><au>Margolles, Abelardo</au><au>Mayo, Baltasar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of tetracycline resistance tet(W) genes and their flanking sequences in intestinal Bifidobacterium species</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>62</volume><issue>4</issue><spage>688</spage><epage>693</epage><pages>688-693</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives The tet(W) gene provides tetracycline resistance to a wide range of anaerobic intestinal and ruminal bacteria, but little is known about the molecular organization of the tet(W) gene. The aim of this study was to gain new insights into the molecular organization of the tet(W) gene in bifidobacteria strains from humans. Methods A segment of DNA encompassing the whole tet(W) gene and its immediate upstream and downstream sequences was analysed in 10 representative strains of four Bifidobacterium species, of which two have been shown to be tetracycline-susceptible. The non-conserved flanking regions of the tet(W) gene were further analysed in six strains. Results All 10 strains share a core DNA domain of 2154 bp [starting 250 bp upstream of the tet(W) gene start codon and ending 13 bp before the stop codon] with 98% to 100% DNA identity. Except for Bifidobacterium animalis E43, all other strains further share 408 bp upstream and 70 bp downstream of the tet(W) gene. An insertion-like element of 736 bp was found to interrupt the tet(W) coding sequence in Bifidobacterium longum M21, which may be the reason for its tetracycline susceptibility. However, genetic events explaining the susceptible phenotype of B. longum LMG 13197T were not observed. Conclusions The tet(W) genes from all 10 strains shared 98% to 100% DNA and amino acid identity, though large variation was found in their flanking regions.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>18614524</pmid><doi>10.1093/jac/dkn280</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anti-Bacterial Agents - pharmacology antibiotic resistance Antibiotics. Antiinfectious agents. Antiparasitic agents Bacteria Bacterial Proteins - genetics bifidobacteria Bifidobacterium - genetics Bifidobacterium - isolation & purification Bifidobacterium animalis Bifidobacterium longum Biological and medical sciences Deoxyribonucleic acid DNA DNA Transposable Elements DNA, Bacterial - chemistry DNA, Bacterial - genetics Drug resistance Gastrointestinal Tract - microbiology Genetics Humans Medical sciences Molecular biology Molecular Sequence Data Mutagenesis, Insertional Pharmacology. Drug treatments probiotics Recombination, Genetic Sequence Analysis, DNA Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Tetracycline - pharmacology Tetracycline Resistance |
| title | Analysis of tetracycline resistance tet(W) genes and their flanking sequences in intestinal Bifidobacterium species |
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