Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe

The ease of construction of multiple mutant strains in Schizosaccharomyces pombe is limited by the number of available genetic markers. We describe here three new cassettes for PCR‐mediated gene disruption that can be used in combination with commonly used fission yeast markers to make multiple gene...

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Veröffentlicht in:	Yeast (Chichester, England) England), 2005-10, Vol.22 (13), p.1013-1019
Hauptverfasser:	Hentges, Pierre, Van Driessche, Benoit, Tafforeau, Lionel, Vandenhaute, Jean, Carr, Antony M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Antifungal Agents - pharmacology Bacterial Proteins - genetics Drug Resistance, Fungal - genetics Gene Deletion gene disruption Genetic Markers hygromycin B Hygromycin B - pharmacology nourseothricin phleomycin Phleomycins - pharmacology Schizosaccharomyces - drug effects Schizosaccharomyces - genetics Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins - genetics selectable markers Streptothricins - pharmacology Transformation, Genetic
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