Effects of lycorine on HL-60 cells via arresting cell cycle and inducing apoptosis

As a natural anti-cancer alkaloid extracted from Amaryllidaceae, lycorine shows various biological effects on tumor cells. The survival rate of HL-60 cells exposed to lycorine was decreased in a dose-dependent manner with 1 μM as the 50% inhibitory concentration (IC50), cell growth was slowed down b...

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Veröffentlicht in:	FEBS letters 2004-12, Vol.578 (3), p.245-250
Hauptverfasser:	Liu, Jing, Hu, Wei-Xin, He, Li-Fang, Ye, Mao, Li, Yan
Format:	Artikel
Sprache:	eng
Schlagworte:	3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide 50% inhibitory concentration acridine orange acute promyelocytic leukemia Amaryllidaceae Amaryllidaceae Alkaloids - pharmacology Antineoplastic Agents, Phytogenic - pharmacology APL Apoptosis Apoptosis - drug effects Asp-Glu-Val-Asp conjugated to p-nitroanilide bcl-2-Associated X Protein Blotting, Western Caspases - analysis Caspases - drug effects Caspases - metabolism Cell cycle Cell Cycle - drug effects Cell Survival Colorimetry Cytotoxicity Tests, Immunologic DEVD-pNA dimethyl sulfoxide DMSO Dose-Response Relationship, Drug Enzyme Activation - drug effects ethidium bromide Flow Cytometry G2 Phase - drug effects Gene Expression Regulation, Neoplastic - drug effects HL-60 cell line HL-60 Cells Humans IC50 IETD-pNA Ile-Glu-Thr-Asp conjugated to p-nitroanilide Inhibitory Concentration 50 LEHD-pNA Leu-Glu-His-Asp conjugated to p-nitroanilide Leukemia Leukemia, Promyelocytic, Acute - drug therapy Lycorine Microscopy, Fluorescence MTT PBS Phenanthridines - pharmacology phosphate-buffered saline propidum iodide Proto-Oncogene Proteins c-bcl-2 - analysis Proto-Oncogene Proteins c-bcl-2 - drug effects Proto-Oncogene Proteins c-bcl-2 - metabolism Time Factors
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