The differential expression of novel circular RNAs in an acute lung injury rat model caused by smoke inhalation
Acute lung injury caused by smoke inhalation is a common severe clinical syndrome. This study aimed to investigate the potential expression of circular RNAs during acute lung injury triggered by smoke inhalation. The acute lung injury rat model was established with smoke inhalation from a self-made...
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| Veröffentlicht in: | Journal of physiology and biochemistry 2018-02, Vol.74 (1), p.25-33 |
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| container_title | Journal of physiology and biochemistry |
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| creator | Ye, Zhiqiang Liu, Xuhui Yang, Yuewu Zhang, Xianling Yu, Ting Li, Shigeng Feng, Yawei Luo, Gangjian |
| description | Acute lung injury caused by smoke inhalation is a common severe clinical syndrome. This study aimed to investigate the potential expression of circular RNAs during acute lung injury triggered by smoke inhalation. The acute lung injury rat model was established with smoke inhalation from a self-made smoke generator. The occurrence of acute lung injury was validated by an analysis of the bronchoalveolar lavage fluid and hematoxylin-eosin (HE) staining of lung tissues. Next-generation sequencing and quantitative PCR were performed to identify the differentially expressed circular RNAs associated with acute lung injury that was caused by smoke inhalation. The circular form of the identified RNAs was finally verified by multiple RT-PCR-based assays. The bronchoalveolar lavage fluid (BALF) and lung tissue analysis showed that smoke inhalation successfully induced acute injury in rats, as evidenced by the significantly altered cell numbers, including macrophages, neutrophils, and red blood cells, disrupted cell lining, and increased levels of interleukin-1β, tumor necrosis factor-alpha, and IL-8 in lung tissues. Ten significantly differentially expressed circular RNAs were identified with next-generation sequencing and RT-PCR. The circular form of these RNAs was verified by multiple RT-PCR-based assays. In conclusion, the identified circular RNAs were prevalently and differentially expressed in rat lungs after acute lung injury caused by smoke inhalation. |
| doi_str_mv | 10.1007/s13105-017-0598-5 |
| format | Article |
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This study aimed to investigate the potential expression of circular RNAs during acute lung injury triggered by smoke inhalation. The acute lung injury rat model was established with smoke inhalation from a self-made smoke generator. The occurrence of acute lung injury was validated by an analysis of the bronchoalveolar lavage fluid and hematoxylin-eosin (HE) staining of lung tissues. Next-generation sequencing and quantitative PCR were performed to identify the differentially expressed circular RNAs associated with acute lung injury that was caused by smoke inhalation. The circular form of the identified RNAs was finally verified by multiple RT-PCR-based assays. The bronchoalveolar lavage fluid (BALF) and lung tissue analysis showed that smoke inhalation successfully induced acute injury in rats, as evidenced by the significantly altered cell numbers, including macrophages, neutrophils, and red blood cells, disrupted cell lining, and increased levels of interleukin-1β, tumor necrosis factor-alpha, and IL-8 in lung tissues. Ten significantly differentially expressed circular RNAs were identified with next-generation sequencing and RT-PCR. The circular form of these RNAs was verified by multiple RT-PCR-based assays. In conclusion, the identified circular RNAs were prevalently and differentially expressed in rat lungs after acute lung injury caused by smoke inhalation.</description><identifier>ISSN: 1138-7548</identifier><identifier>EISSN: 1877-8755</identifier><identifier>DOI: 10.1007/s13105-017-0598-5</identifier><identifier>PMID: 29188496</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alveoli ; Animal Physiology ; Biomedical and Life Sciences ; Biomedicine ; Bronchus ; Circularity ; Erythrocytes ; Human Physiology ; Inhalation ; Injuries ; Injury analysis ; Interleukin 8 ; Leukocytes (neutrophilic) ; Lungs ; Macrophages ; Original Article ; Polymerase chain reaction ; Rats ; Respiration ; Rodents ; Smoke ; Smoke inhalation ; Tumor necrosis factor-α</subject><ispartof>Journal of physiology and biochemistry, 2018-02, Vol.74 (1), p.25-33</ispartof><rights>University of Navarra 2017</rights><rights>Copyright Springer Science & Business Media 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-ab3ba260c32453414d64aad772522cf4b7ebccf202b679760cd44445ea0ad1123</citedby><cites>FETCH-LOGICAL-c372t-ab3ba260c32453414d64aad772522cf4b7ebccf202b679760cd44445ea0ad1123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s13105-017-0598-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s13105-017-0598-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29188496$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ye, Zhiqiang</creatorcontrib><creatorcontrib>Liu, Xuhui</creatorcontrib><creatorcontrib>Yang, Yuewu</creatorcontrib><creatorcontrib>Zhang, Xianling</creatorcontrib><creatorcontrib>Yu, Ting</creatorcontrib><creatorcontrib>Li, Shigeng</creatorcontrib><creatorcontrib>Feng, Yawei</creatorcontrib><creatorcontrib>Luo, Gangjian</creatorcontrib><title>The differential expression of novel circular RNAs in an acute lung injury rat model caused by smoke inhalation</title><title>Journal of physiology and biochemistry</title><addtitle>J Physiol Biochem</addtitle><addtitle>J Physiol Biochem</addtitle><description>Acute lung injury caused by smoke inhalation is a common severe clinical syndrome. This study aimed to investigate the potential expression of circular RNAs during acute lung injury triggered by smoke inhalation. The acute lung injury rat model was established with smoke inhalation from a self-made smoke generator. The occurrence of acute lung injury was validated by an analysis of the bronchoalveolar lavage fluid and hematoxylin-eosin (HE) staining of lung tissues. Next-generation sequencing and quantitative PCR were performed to identify the differentially expressed circular RNAs associated with acute lung injury that was caused by smoke inhalation. The circular form of the identified RNAs was finally verified by multiple RT-PCR-based assays. The bronchoalveolar lavage fluid (BALF) and lung tissue analysis showed that smoke inhalation successfully induced acute injury in rats, as evidenced by the significantly altered cell numbers, including macrophages, neutrophils, and red blood cells, disrupted cell lining, and increased levels of interleukin-1β, tumor necrosis factor-alpha, and IL-8 in lung tissues. Ten significantly differentially expressed circular RNAs were identified with next-generation sequencing and RT-PCR. The circular form of these RNAs was verified by multiple RT-PCR-based assays. In conclusion, the identified circular RNAs were prevalently and differentially expressed in rat lungs after acute lung injury caused by smoke inhalation.</description><subject>Alveoli</subject><subject>Animal Physiology</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bronchus</subject><subject>Circularity</subject><subject>Erythrocytes</subject><subject>Human Physiology</subject><subject>Inhalation</subject><subject>Injuries</subject><subject>Injury analysis</subject><subject>Interleukin 8</subject><subject>Leukocytes (neutrophilic)</subject><subject>Lungs</subject><subject>Macrophages</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Rats</subject><subject>Respiration</subject><subject>Rodents</subject><subject>Smoke</subject><subject>Smoke inhalation</subject><subject>Tumor necrosis factor-α</subject><issn>1138-7548</issn><issn>1877-8755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kdtKxDAQhoMoHlYfwBsJeONNNcemvRTxBKIgeh3SdLrbtW3WpBH37c26KiI4BGZIvvknzI_QISWnlBB1FiinRGaEqozIssjkBtqlhVJZoaTcTDXlRaakKHbQXghzQgSjjGyjHVbSohBlvovc0wxw3TYNeBjG1nQY3hceQmjdgF2DB_cGHbatt7EzHj_enwfcDtikY-MIuIvDNF3Mo19ib0bcu3rFmxigxtUSh969QAJmpjNj0txHW43pAhx85Ql6vrp8urjJ7h6uby_O7zLLFRszU_HKsJxYzoTkgoo6F8bUSjHJmG1EpaCytmGEVbkqVQJrkUKCIaamlPEJOlnrLrx7jRBG3bfBQteZAVwMmpaK5JyIT_T4Dzp30Q_pd4kqS8VynhY5QXRNWe9C8NDohW9745eaEr1yQ6_d0MkNvXJDy9Rz9KUcqx7qn47v9SeArYGQnoYp-F-j_1X9AMgnlM4</recordid><startdate>20180201</startdate><enddate>20180201</enddate><creator>Ye, Zhiqiang</creator><creator>Liu, Xuhui</creator><creator>Yang, Yuewu</creator><creator>Zhang, Xianling</creator><creator>Yu, Ting</creator><creator>Li, Shigeng</creator><creator>Feng, Yawei</creator><creator>Luo, Gangjian</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180201</creationdate><title>The differential expression of novel circular RNAs in an acute lung injury rat model caused by smoke inhalation</title><author>Ye, Zhiqiang ; Liu, Xuhui ; Yang, Yuewu ; Zhang, Xianling ; Yu, Ting ; Li, Shigeng ; Feng, Yawei ; Luo, Gangjian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-ab3ba260c32453414d64aad772522cf4b7ebccf202b679760cd44445ea0ad1123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alveoli</topic><topic>Animal Physiology</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bronchus</topic><topic>Circularity</topic><topic>Erythrocytes</topic><topic>Human Physiology</topic><topic>Inhalation</topic><topic>Injuries</topic><topic>Injury analysis</topic><topic>Interleukin 8</topic><topic>Leukocytes (neutrophilic)</topic><topic>Lungs</topic><topic>Macrophages</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Rats</topic><topic>Respiration</topic><topic>Rodents</topic><topic>Smoke</topic><topic>Smoke inhalation</topic><topic>Tumor necrosis factor-α</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ye, Zhiqiang</creatorcontrib><creatorcontrib>Liu, Xuhui</creatorcontrib><creatorcontrib>Yang, Yuewu</creatorcontrib><creatorcontrib>Zhang, Xianling</creatorcontrib><creatorcontrib>Yu, Ting</creatorcontrib><creatorcontrib>Li, Shigeng</creatorcontrib><creatorcontrib>Feng, Yawei</creatorcontrib><creatorcontrib>Luo, Gangjian</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of physiology and biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ye, Zhiqiang</au><au>Liu, Xuhui</au><au>Yang, Yuewu</au><au>Zhang, Xianling</au><au>Yu, Ting</au><au>Li, Shigeng</au><au>Feng, Yawei</au><au>Luo, Gangjian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The differential expression of novel circular RNAs in an acute lung injury rat model caused by smoke inhalation</atitle><jtitle>Journal of physiology and biochemistry</jtitle><stitle>J Physiol Biochem</stitle><addtitle>J Physiol Biochem</addtitle><date>2018-02-01</date><risdate>2018</risdate><volume>74</volume><issue>1</issue><spage>25</spage><epage>33</epage><pages>25-33</pages><issn>1138-7548</issn><eissn>1877-8755</eissn><abstract>Acute lung injury caused by smoke inhalation is a common severe clinical syndrome. This study aimed to investigate the potential expression of circular RNAs during acute lung injury triggered by smoke inhalation. The acute lung injury rat model was established with smoke inhalation from a self-made smoke generator. The occurrence of acute lung injury was validated by an analysis of the bronchoalveolar lavage fluid and hematoxylin-eosin (HE) staining of lung tissues. Next-generation sequencing and quantitative PCR were performed to identify the differentially expressed circular RNAs associated with acute lung injury that was caused by smoke inhalation. The circular form of the identified RNAs was finally verified by multiple RT-PCR-based assays. The bronchoalveolar lavage fluid (BALF) and lung tissue analysis showed that smoke inhalation successfully induced acute injury in rats, as evidenced by the significantly altered cell numbers, including macrophages, neutrophils, and red blood cells, disrupted cell lining, and increased levels of interleukin-1β, tumor necrosis factor-alpha, and IL-8 in lung tissues. Ten significantly differentially expressed circular RNAs were identified with next-generation sequencing and RT-PCR. The circular form of these RNAs was verified by multiple RT-PCR-based assays. In conclusion, the identified circular RNAs were prevalently and differentially expressed in rat lungs after acute lung injury caused by smoke inhalation.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>29188496</pmid><doi>10.1007/s13105-017-0598-5</doi><tpages>9</tpages></addata></record> |
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| subjects | Alveoli Animal Physiology Biomedical and Life Sciences Biomedicine Bronchus Circularity Erythrocytes Human Physiology Inhalation Injuries Injury analysis Interleukin 8 Leukocytes (neutrophilic) Lungs Macrophages Original Article Polymerase chain reaction Rats Respiration Rodents Smoke Smoke inhalation Tumor necrosis factor-α |
| title | The differential expression of novel circular RNAs in an acute lung injury rat model caused by smoke inhalation |
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