Influence of genetic polymorphisms of styrene-metabolizing enzymes and smoking habits on levels of urinary metabolites after occupational exposure to styrene
Here we evaluate the influence of individual genetic polymorphisms of drug-metabolizing enzymes as well as body mass index (BMI) and lifestyle (smoking, alcohol consumption) on urinary metabolites after occupational exposure to styrene. Seventy-three workers exposed to styrene in a reinforced-plasti...
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| Veröffentlicht in: | Toxicology letters 2005-12, Vol.160 (1), p.84-91 |
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| creator | Ma, Mingyue Umemura, Tomohiro Mori, Yuko Gong, Yingyan Saijo, Yasuaki Sata, Fumihiro Kawai, Toshio Kishi, Reiko |
| description | Here we evaluate the influence of individual genetic polymorphisms of drug-metabolizing enzymes as well as body mass index (BMI) and lifestyle (smoking, alcohol consumption) on urinary metabolites after occupational exposure to styrene. Seventy-three workers exposed to styrene in a reinforced-plastics workplace were studied. The personal styrene exposure in the air and the urinary styrene metabolites mandelic acid and phenylglyoxylic acid were measured. The subjects’ genetic polymorphisms in the genes that encode the styrene-metabolizing enzymes
CYP2E1,
CYP2B6,
EPHX1,
GSTM1,
GSTT1 and
GSTP1 were determined. Neither genotype nor lifestyle significantly affected urinary metabolites. There was, however, an interaction between the
CYP2E1 genotype and smoking. Among non-smokers, urinary styrene metabolites were significantly decreased in subjects with c1/c1 alleles of
CYP2E1 as compared with those with the c1/c2 genotype. There was no significant difference in urinary metabolites among smokers. When the combined influence of the
CYP2B6 genotype and the predicted activity of
EPHX1 were examined, urinary metabolites in subjects with low enzyme activity were lower than in those with medium or high activity after high styrene exposure (≥50
ppm). The results suggest that genetic susceptibility and lifestyle should be considered in biological monitoring of exposure to styrene. |
| doi_str_mv | 10.1016/j.toxlet.2005.06.011 |
| format | Article |
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CYP2E1,
CYP2B6,
EPHX1,
GSTM1,
GSTT1 and
GSTP1 were determined. Neither genotype nor lifestyle significantly affected urinary metabolites. There was, however, an interaction between the
CYP2E1 genotype and smoking. Among non-smokers, urinary styrene metabolites were significantly decreased in subjects with c1/c1 alleles of
CYP2E1 as compared with those with the c1/c2 genotype. There was no significant difference in urinary metabolites among smokers. When the combined influence of the
CYP2B6 genotype and the predicted activity of
EPHX1 were examined, urinary metabolites in subjects with low enzyme activity were lower than in those with medium or high activity after high styrene exposure (≥50
ppm). The results suggest that genetic susceptibility and lifestyle should be considered in biological monitoring of exposure to styrene.</description><identifier>ISSN: 0378-4274</identifier><identifier>EISSN: 1879-3169</identifier><identifier>DOI: 10.1016/j.toxlet.2005.06.011</identifier><identifier>PMID: 16125881</identifier><identifier>CODEN: TOLED5</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Adult ; Alcohol Drinking - urine ; Aryl Hydrocarbon Hydroxylases - genetics ; Aryl Hydrocarbon Hydroxylases - metabolism ; Biological and medical sciences ; Biological monitoring ; Body Mass Index ; Chemical and industrial products toxicology. Toxic occupational diseases ; Cytochrome P-450 CYP2B6 ; Cytochrome P-450 CYP2E1 - genetics ; Cytochrome P-450 CYP2E1 - metabolism ; Epoxide Hydrolases - genetics ; Epoxide Hydrolases - metabolism ; Genetic polymorphisms ; Genotype ; Glutathione S-Transferase pi - genetics ; Glutathione S-Transferase pi - metabolism ; Glutathione Transferase - genetics ; Glutathione Transferase - metabolism ; Glyoxylates - urine ; Humans ; Male ; Mandelic acid (MA) ; Mandelic Acids - urine ; Medical sciences ; Occupational Exposure - adverse effects ; Oxidoreductases, N-Demethylating - genetics ; Oxidoreductases, N-Demethylating - metabolism ; Phenylglyoxylic acid (PGA) ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Smoking - urine ; Solvents ; Styrene ; Styrene - metabolism ; Styrene - poisoning ; Tobacco, tobacco smoking ; Toxicology</subject><ispartof>Toxicology letters, 2005-12, Vol.160 (1), p.84-91</ispartof><rights>2005 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-81f050c772ded5ab2f8cd0a2b3ec00d862473857539c3e7a9dbf4c61af7ed9113</citedby><cites>FETCH-LOGICAL-c487t-81f050c772ded5ab2f8cd0a2b3ec00d862473857539c3e7a9dbf4c61af7ed9113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378427405001864$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17226514$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16125881$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Mingyue</creatorcontrib><creatorcontrib>Umemura, Tomohiro</creatorcontrib><creatorcontrib>Mori, Yuko</creatorcontrib><creatorcontrib>Gong, Yingyan</creatorcontrib><creatorcontrib>Saijo, Yasuaki</creatorcontrib><creatorcontrib>Sata, Fumihiro</creatorcontrib><creatorcontrib>Kawai, Toshio</creatorcontrib><creatorcontrib>Kishi, Reiko</creatorcontrib><title>Influence of genetic polymorphisms of styrene-metabolizing enzymes and smoking habits on levels of urinary metabolites after occupational exposure to styrene</title><title>Toxicology letters</title><addtitle>Toxicol Lett</addtitle><description>Here we evaluate the influence of individual genetic polymorphisms of drug-metabolizing enzymes as well as body mass index (BMI) and lifestyle (smoking, alcohol consumption) on urinary metabolites after occupational exposure to styrene. Seventy-three workers exposed to styrene in a reinforced-plastics workplace were studied. The personal styrene exposure in the air and the urinary styrene metabolites mandelic acid and phenylglyoxylic acid were measured. The subjects’ genetic polymorphisms in the genes that encode the styrene-metabolizing enzymes
CYP2E1,
CYP2B6,
EPHX1,
GSTM1,
GSTT1 and
GSTP1 were determined. Neither genotype nor lifestyle significantly affected urinary metabolites. There was, however, an interaction between the
CYP2E1 genotype and smoking. Among non-smokers, urinary styrene metabolites were significantly decreased in subjects with c1/c1 alleles of
CYP2E1 as compared with those with the c1/c2 genotype. There was no significant difference in urinary metabolites among smokers. When the combined influence of the
CYP2B6 genotype and the predicted activity of
EPHX1 were examined, urinary metabolites in subjects with low enzyme activity were lower than in those with medium or high activity after high styrene exposure (≥50
ppm). The results suggest that genetic susceptibility and lifestyle should be considered in biological monitoring of exposure to styrene.</description><subject>Adult</subject><subject>Alcohol Drinking - urine</subject><subject>Aryl Hydrocarbon Hydroxylases - genetics</subject><subject>Aryl Hydrocarbon Hydroxylases - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological monitoring</subject><subject>Body Mass Index</subject><subject>Chemical and industrial products toxicology. Toxic occupational diseases</subject><subject>Cytochrome P-450 CYP2B6</subject><subject>Cytochrome P-450 CYP2E1 - genetics</subject><subject>Cytochrome P-450 CYP2E1 - metabolism</subject><subject>Epoxide Hydrolases - genetics</subject><subject>Epoxide Hydrolases - metabolism</subject><subject>Genetic polymorphisms</subject><subject>Genotype</subject><subject>Glutathione S-Transferase pi - genetics</subject><subject>Glutathione S-Transferase pi - metabolism</subject><subject>Glutathione Transferase - genetics</subject><subject>Glutathione Transferase - metabolism</subject><subject>Glyoxylates - urine</subject><subject>Humans</subject><subject>Male</subject><subject>Mandelic acid (MA)</subject><subject>Mandelic Acids - urine</subject><subject>Medical sciences</subject><subject>Occupational Exposure - adverse effects</subject><subject>Oxidoreductases, N-Demethylating - genetics</subject><subject>Oxidoreductases, N-Demethylating - metabolism</subject><subject>Phenylglyoxylic acid (PGA)</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Genetic</subject><subject>Smoking - urine</subject><subject>Solvents</subject><subject>Styrene</subject><subject>Styrene - metabolism</subject><subject>Styrene - poisoning</subject><subject>Tobacco, tobacco smoking</subject><subject>Toxicology</subject><issn>0378-4274</issn><issn>1879-3169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi0EokPhDRDyBnYJx7nYyQYJVVwqVWIDa8uxT1oPjh1sp-r0XXhXks5U3bGy5PP9vy8fIW8ZlAwY_7gvc7hzmMsKoC2Bl8DYM7JjneiLmvH-OdlBLbqiqURzRl6ltAcA3vD2JTljnFVt17Ed-XvpR7eg10jDSK_RY7aazsEdphDnG5umtA1SPsR1VkyY1RCcvbf-mqK_P0yYqPKGpin83vZu1GDzGvHU4S26h_ASrVfxQB_DecuMGSMNWi-zyjZ45SjezSEtEWkOj-e9Ji9G5RK-Oa3n5NfXLz8vvhdXP75dXny-KnTTiVx0bIQWtBCVQdOqoRo7bUBVQ40awHS8akTdtaKte12jUL0ZxkZzpkaBpmesPicfjr1zDH8WTFlONml0TnkMS5KsF8ChgRVsjqCOIaWIo5yjndbHSQZy0yL38qhFblokcAkP_e9O_cswoXkKnTyswPsToJJWbozKa5ueOFFVvGXNyn06cuvX4q3FKJO2mz1jI-osTbD_v8k_dMqy_w</recordid><startdate>20051230</startdate><enddate>20051230</enddate><creator>Ma, Mingyue</creator><creator>Umemura, Tomohiro</creator><creator>Mori, Yuko</creator><creator>Gong, Yingyan</creator><creator>Saijo, Yasuaki</creator><creator>Sata, Fumihiro</creator><creator>Kawai, Toshio</creator><creator>Kishi, Reiko</creator><general>Elsevier Ireland Ltd</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T2</scope><scope>7U2</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20051230</creationdate><title>Influence of genetic polymorphisms of styrene-metabolizing enzymes and smoking habits on levels of urinary metabolites after occupational exposure to styrene</title><author>Ma, Mingyue ; Umemura, Tomohiro ; Mori, Yuko ; Gong, Yingyan ; Saijo, Yasuaki ; Sata, Fumihiro ; Kawai, Toshio ; Kishi, Reiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-81f050c772ded5ab2f8cd0a2b3ec00d862473857539c3e7a9dbf4c61af7ed9113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adult</topic><topic>Alcohol Drinking - urine</topic><topic>Aryl Hydrocarbon Hydroxylases - genetics</topic><topic>Aryl Hydrocarbon Hydroxylases - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biological monitoring</topic><topic>Body Mass Index</topic><topic>Chemical and industrial products toxicology. Toxic occupational diseases</topic><topic>Cytochrome P-450 CYP2B6</topic><topic>Cytochrome P-450 CYP2E1 - genetics</topic><topic>Cytochrome P-450 CYP2E1 - metabolism</topic><topic>Epoxide Hydrolases - genetics</topic><topic>Epoxide Hydrolases - metabolism</topic><topic>Genetic polymorphisms</topic><topic>Genotype</topic><topic>Glutathione S-Transferase pi - genetics</topic><topic>Glutathione S-Transferase pi - metabolism</topic><topic>Glutathione Transferase - genetics</topic><topic>Glutathione Transferase - metabolism</topic><topic>Glyoxylates - urine</topic><topic>Humans</topic><topic>Male</topic><topic>Mandelic acid (MA)</topic><topic>Mandelic Acids - urine</topic><topic>Medical sciences</topic><topic>Occupational Exposure - adverse effects</topic><topic>Oxidoreductases, N-Demethylating - genetics</topic><topic>Oxidoreductases, N-Demethylating - metabolism</topic><topic>Phenylglyoxylic acid (PGA)</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Genetic</topic><topic>Smoking - urine</topic><topic>Solvents</topic><topic>Styrene</topic><topic>Styrene - metabolism</topic><topic>Styrene - poisoning</topic><topic>Tobacco, tobacco smoking</topic><topic>Toxicology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Mingyue</creatorcontrib><creatorcontrib>Umemura, Tomohiro</creatorcontrib><creatorcontrib>Mori, Yuko</creatorcontrib><creatorcontrib>Gong, Yingyan</creatorcontrib><creatorcontrib>Saijo, Yasuaki</creatorcontrib><creatorcontrib>Sata, Fumihiro</creatorcontrib><creatorcontrib>Kawai, Toshio</creatorcontrib><creatorcontrib>Kishi, Reiko</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Safety Science and Risk</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Toxicology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Mingyue</au><au>Umemura, Tomohiro</au><au>Mori, Yuko</au><au>Gong, Yingyan</au><au>Saijo, Yasuaki</au><au>Sata, Fumihiro</au><au>Kawai, Toshio</au><au>Kishi, Reiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of genetic polymorphisms of styrene-metabolizing enzymes and smoking habits on levels of urinary metabolites after occupational exposure to styrene</atitle><jtitle>Toxicology letters</jtitle><addtitle>Toxicol Lett</addtitle><date>2005-12-30</date><risdate>2005</risdate><volume>160</volume><issue>1</issue><spage>84</spage><epage>91</epage><pages>84-91</pages><issn>0378-4274</issn><eissn>1879-3169</eissn><coden>TOLED5</coden><abstract>Here we evaluate the influence of individual genetic polymorphisms of drug-metabolizing enzymes as well as body mass index (BMI) and lifestyle (smoking, alcohol consumption) on urinary metabolites after occupational exposure to styrene. Seventy-three workers exposed to styrene in a reinforced-plastics workplace were studied. The personal styrene exposure in the air and the urinary styrene metabolites mandelic acid and phenylglyoxylic acid were measured. The subjects’ genetic polymorphisms in the genes that encode the styrene-metabolizing enzymes
CYP2E1,
CYP2B6,
EPHX1,
GSTM1,
GSTT1 and
GSTP1 were determined. Neither genotype nor lifestyle significantly affected urinary metabolites. There was, however, an interaction between the
CYP2E1 genotype and smoking. Among non-smokers, urinary styrene metabolites were significantly decreased in subjects with c1/c1 alleles of
CYP2E1 as compared with those with the c1/c2 genotype. There was no significant difference in urinary metabolites among smokers. When the combined influence of the
CYP2B6 genotype and the predicted activity of
EPHX1 were examined, urinary metabolites in subjects with low enzyme activity were lower than in those with medium or high activity after high styrene exposure (≥50
ppm). The results suggest that genetic susceptibility and lifestyle should be considered in biological monitoring of exposure to styrene.</abstract><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier Ireland Ltd</pub><pmid>16125881</pmid><doi>10.1016/j.toxlet.2005.06.011</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-4274 |
| ispartof | Toxicology letters, 2005-12, Vol.160 (1), p.84-91 |
| issn | 0378-4274 1879-3169 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19706040 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adult Alcohol Drinking - urine Aryl Hydrocarbon Hydroxylases - genetics Aryl Hydrocarbon Hydroxylases - metabolism Biological and medical sciences Biological monitoring Body Mass Index Chemical and industrial products toxicology. Toxic occupational diseases Cytochrome P-450 CYP2B6 Cytochrome P-450 CYP2E1 - genetics Cytochrome P-450 CYP2E1 - metabolism Epoxide Hydrolases - genetics Epoxide Hydrolases - metabolism Genetic polymorphisms Genotype Glutathione S-Transferase pi - genetics Glutathione S-Transferase pi - metabolism Glutathione Transferase - genetics Glutathione Transferase - metabolism Glyoxylates - urine Humans Male Mandelic acid (MA) Mandelic Acids - urine Medical sciences Occupational Exposure - adverse effects Oxidoreductases, N-Demethylating - genetics Oxidoreductases, N-Demethylating - metabolism Phenylglyoxylic acid (PGA) Polymerase Chain Reaction Polymorphism, Genetic Smoking - urine Solvents Styrene Styrene - metabolism Styrene - poisoning Tobacco, tobacco smoking Toxicology |
| title | Influence of genetic polymorphisms of styrene-metabolizing enzymes and smoking habits on levels of urinary metabolites after occupational exposure to styrene |
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