Development of microsatellite markers in bonytail (Gila elegans) with cross-species amplification in humpback chub (Gila cypha)

Development of microsatellite markers in bonytail (Gila elegans) with cross-species amplification in humpback chub (Gila cypha)

Bonytail, Gila elegans, is an endangered species of fish native to the Colorado River. Primers are presented for 17 microsatellites cloned from bonytail as well as the results of test amplifications in bonytail and humpback chub, G. cypha. Bonytail exhibited three to 18 alleles per locus across the...

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Veröffentlicht in:Molecular ecology notes 2004-03, Vol.4 (1), p.23-25
Hauptverfasser: Keeler-Foster, Connie L., Spies, Ingrid B., Bondu-Hawkins, Virginie, Bentzen, Paul
Format: Artikel
Sprache:eng
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bonytail
Dexter National Fish Hatchery and Technology Center
enrichment
Gila
Gila cypha
Gila elegans
humpback
microsatellites
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container_issue 1
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container_title Molecular ecology notes
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creator Keeler-Foster, Connie L.
Spies, Ingrid B.
Bondu-Hawkins, Virginie
Bentzen, Paul
description Bonytail, Gila elegans, is an endangered species of fish native to the Colorado River. Primers are presented for 17 microsatellites cloned from bonytail as well as the results of test amplifications in bonytail and humpback chub, G. cypha. Bonytail exhibited three to 18 alleles per locus across the 17 microsatellites and a mean expected heterozygosity of 0.58 among 10 loci used to screen 160 broodstock. Humpback chub exhibited one to six alleles and a mean expected heterozygosity of 0.69 among 10 loci that were successfully amplified in that species.
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1471-8286
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source Wiley Online Library - AutoHoldings Journals
subjects bonytail
Dexter National Fish Hatchery and Technology Center
enrichment
Gila
Gila cypha
Gila elegans
humpback
microsatellites
title Development of microsatellite markers in bonytail (Gila elegans) with cross-species amplification in humpback chub (Gila cypha)
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