Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling
1 Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative (CRIBI) Biotechnology Center, University of Padova, Padua, Italy 2 Department of Anatomy and Physiology, University of Padova, Padua, Italy Denervation deeply affects muscle structure and function, the alterations being differen...
Gespeichert in:
| Veröffentlicht in: | Physiological genomics 2006-03, Vol.25 (1), p.60-74 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 74 |
|---|---|
| container_issue | 1 |
| container_start_page | 60 |
| container_title | Physiological genomics |
| container_volume | 25 |
| creator | Raffaello, Anna Laveder, Paolo Romualdi, Chiara Bean, Camilla Toniolo, Luana Germinario, Elena Megighian, Aram Danieli-Betto, Daniela Reggiani, Carlo Lanfranchi, Gerolamo |
| description | 1 Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative (CRIBI) Biotechnology Center, University of Padova, Padua, Italy
2 Department of Anatomy and Physiology, University of Padova, Padua, Italy
Denervation deeply affects muscle structure and function, the alterations being different in slow and fast muscles. Because the effects of denervation on fast muscles are still controversial, and high-throughput studies on gene expression in denervated muscles are lacking, we studied gene expression during atrophy progression following denervation in mouse tibialis anterior (TA). The sciatic nerve was cut close to trochanter in adult CD1 mice. One, three, seven, and fourteen days after denervation, animals were killed and TA muscles were dissected out and utilized for physiological experiments and gene expression studies. Target cDNAs from TA muscles were hybridized on a dedicated cDNA microarray of muscle genes. Seventy-one genes were found differentially expressed. Microarray results were validated, and the expression of relevant genes not probed on our array was monitored by real-time quantitative PCR (RQ-PCR). Nuclear- and mitochondrial-encoded genes implicated in energy metabolism were consistently downregulated. Among genes implicated in muscle contraction (myofibrillar and sarcoplasmic reticulum), genes typical of fast fibers were downregulated, whereas those typical of slow fibers were upregulated. Electrophoresis and Western blot showed less pronounced changes in myofibrillar protein expression, partially confirming changes in gene expression. Isometric tension of skinned fibers was little affected by denervation, whereas calcium sensitivity decreased. Functional studies in mouse extensor digitorum longus muscle showed prolongation in twitch time parameters and shift to the left in force-frequency curves after denervation. We conclude that, if studied at the mRNA level, fast muscles appear not less responsive than slow muscles to the interruption of neural stimulation.
denervation; cDNA microarray; mitochondria; myosin |
| doi_str_mv | 10.1152/physiolgenomics.00051.2005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19699535</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67745729</sourcerecordid><originalsourceid>FETCH-LOGICAL-c512t-6cd9893796a37f6c7f5c5e6b539574c3b0d4915fef66528fc2e7bfda9ff2f6413</originalsourceid><addsrcrecordid>eNqFkU-L1TAUxYsozjj6FaS4cNdn_jRJMwtBRkeFATfjOuSlN20kTWrSOvO-vXm-ojAibpJwOL9zLzlV9QqjHcaMvJnHQ3bRDxDi5EzeIYQY3pFyPqrOMaO4IYSLx-WNZNt0tMVn1bOcvyGEW9Gxp9UZ5rRDLerOK_8eAqQfenEx1C7U05pcgNrqvDTLnVvMWKRsPFzWeYypiJCmetsgDs5oX5tRhwFyrUNfLzDNMRUR7ucEOR9j5xSt8y4Mz6snVvsML7b7ovp6_eH26lNz8-Xj56t3N41hmCwNN73sJBWSayosN8Iyw4DvGZVMtIbuUd9KzCxYzhnprCEg9rbX0lpieYvpRfX6lFsmf18hL2py2YD3OkBcs-JCtEwQ-V8jllxKRlkxXp6MJsWcE1g1JzfpdFAYqWMp6kEp6lcp6lhKgV9uU9b9BP0fdGuhGMjJMLphvHMJ1O8PPvwVTJjCiqMCvf03dL16fwv3y0N6g9XcW_oTX-C7jg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19699535</pqid></control><display><type>article</type><title>Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling</title><source>MEDLINE</source><source>American Physiological Society Paid</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Raffaello, Anna ; Laveder, Paolo ; Romualdi, Chiara ; Bean, Camilla ; Toniolo, Luana ; Germinario, Elena ; Megighian, Aram ; Danieli-Betto, Daniela ; Reggiani, Carlo ; Lanfranchi, Gerolamo</creator><creatorcontrib>Raffaello, Anna ; Laveder, Paolo ; Romualdi, Chiara ; Bean, Camilla ; Toniolo, Luana ; Germinario, Elena ; Megighian, Aram ; Danieli-Betto, Daniela ; Reggiani, Carlo ; Lanfranchi, Gerolamo</creatorcontrib><description>1 Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative (CRIBI) Biotechnology Center, University of Padova, Padua, Italy
2 Department of Anatomy and Physiology, University of Padova, Padua, Italy
Denervation deeply affects muscle structure and function, the alterations being different in slow and fast muscles. Because the effects of denervation on fast muscles are still controversial, and high-throughput studies on gene expression in denervated muscles are lacking, we studied gene expression during atrophy progression following denervation in mouse tibialis anterior (TA). The sciatic nerve was cut close to trochanter in adult CD1 mice. One, three, seven, and fourteen days after denervation, animals were killed and TA muscles were dissected out and utilized for physiological experiments and gene expression studies. Target cDNAs from TA muscles were hybridized on a dedicated cDNA microarray of muscle genes. Seventy-one genes were found differentially expressed. Microarray results were validated, and the expression of relevant genes not probed on our array was monitored by real-time quantitative PCR (RQ-PCR). Nuclear- and mitochondrial-encoded genes implicated in energy metabolism were consistently downregulated. Among genes implicated in muscle contraction (myofibrillar and sarcoplasmic reticulum), genes typical of fast fibers were downregulated, whereas those typical of slow fibers were upregulated. Electrophoresis and Western blot showed less pronounced changes in myofibrillar protein expression, partially confirming changes in gene expression. Isometric tension of skinned fibers was little affected by denervation, whereas calcium sensitivity decreased. Functional studies in mouse extensor digitorum longus muscle showed prolongation in twitch time parameters and shift to the left in force-frequency curves after denervation. We conclude that, if studied at the mRNA level, fast muscles appear not less responsive than slow muscles to the interruption of neural stimulation.
denervation; cDNA microarray; mitochondria; myosin</description><identifier>ISSN: 1094-8341</identifier><identifier>EISSN: 1531-2267</identifier><identifier>DOI: 10.1152/physiolgenomics.00051.2005</identifier><identifier>PMID: 16380408</identifier><language>eng</language><publisher>United States: Am Physiological Soc</publisher><subject>Animals ; Gene Expression Profiling ; Gene Expression Regulation ; Mice ; Mitochondria, Muscle - metabolism ; Mitochondrial Proteins - genetics ; Mitochondrial Proteins - metabolism ; Muscle Denervation ; Muscle Fibers, Fast-Twitch - metabolism ; Muscle Proteins - genetics ; Muscle Proteins - metabolism ; Muscle, Skeletal - innervation ; Muscle, Skeletal - metabolism ; Muscular Atrophy - metabolism ; Oligonucleotide Array Sequence Analysis ; Reproducibility of Results ; RNA, Messenger - metabolism ; Time Factors</subject><ispartof>Physiological genomics, 2006-03, Vol.25 (1), p.60-74</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-6cd9893796a37f6c7f5c5e6b539574c3b0d4915fef66528fc2e7bfda9ff2f6413</citedby><cites>FETCH-LOGICAL-c512t-6cd9893796a37f6c7f5c5e6b539574c3b0d4915fef66528fc2e7bfda9ff2f6413</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3039,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16380408$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Raffaello, Anna</creatorcontrib><creatorcontrib>Laveder, Paolo</creatorcontrib><creatorcontrib>Romualdi, Chiara</creatorcontrib><creatorcontrib>Bean, Camilla</creatorcontrib><creatorcontrib>Toniolo, Luana</creatorcontrib><creatorcontrib>Germinario, Elena</creatorcontrib><creatorcontrib>Megighian, Aram</creatorcontrib><creatorcontrib>Danieli-Betto, Daniela</creatorcontrib><creatorcontrib>Reggiani, Carlo</creatorcontrib><creatorcontrib>Lanfranchi, Gerolamo</creatorcontrib><title>Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling</title><title>Physiological genomics</title><addtitle>Physiol Genomics</addtitle><description>1 Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative (CRIBI) Biotechnology Center, University of Padova, Padua, Italy
2 Department of Anatomy and Physiology, University of Padova, Padua, Italy
Denervation deeply affects muscle structure and function, the alterations being different in slow and fast muscles. Because the effects of denervation on fast muscles are still controversial, and high-throughput studies on gene expression in denervated muscles are lacking, we studied gene expression during atrophy progression following denervation in mouse tibialis anterior (TA). The sciatic nerve was cut close to trochanter in adult CD1 mice. One, three, seven, and fourteen days after denervation, animals were killed and TA muscles were dissected out and utilized for physiological experiments and gene expression studies. Target cDNAs from TA muscles were hybridized on a dedicated cDNA microarray of muscle genes. Seventy-one genes were found differentially expressed. Microarray results were validated, and the expression of relevant genes not probed on our array was monitored by real-time quantitative PCR (RQ-PCR). Nuclear- and mitochondrial-encoded genes implicated in energy metabolism were consistently downregulated. Among genes implicated in muscle contraction (myofibrillar and sarcoplasmic reticulum), genes typical of fast fibers were downregulated, whereas those typical of slow fibers were upregulated. Electrophoresis and Western blot showed less pronounced changes in myofibrillar protein expression, partially confirming changes in gene expression. Isometric tension of skinned fibers was little affected by denervation, whereas calcium sensitivity decreased. Functional studies in mouse extensor digitorum longus muscle showed prolongation in twitch time parameters and shift to the left in force-frequency curves after denervation. We conclude that, if studied at the mRNA level, fast muscles appear not less responsive than slow muscles to the interruption of neural stimulation.
denervation; cDNA microarray; mitochondria; myosin</description><subject>Animals</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation</subject><subject>Mice</subject><subject>Mitochondria, Muscle - metabolism</subject><subject>Mitochondrial Proteins - genetics</subject><subject>Mitochondrial Proteins - metabolism</subject><subject>Muscle Denervation</subject><subject>Muscle Fibers, Fast-Twitch - metabolism</subject><subject>Muscle Proteins - genetics</subject><subject>Muscle Proteins - metabolism</subject><subject>Muscle, Skeletal - innervation</subject><subject>Muscle, Skeletal - metabolism</subject><subject>Muscular Atrophy - metabolism</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Reproducibility of Results</subject><subject>RNA, Messenger - metabolism</subject><subject>Time Factors</subject><issn>1094-8341</issn><issn>1531-2267</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU-L1TAUxYsozjj6FaS4cNdn_jRJMwtBRkeFATfjOuSlN20kTWrSOvO-vXm-ojAibpJwOL9zLzlV9QqjHcaMvJnHQ3bRDxDi5EzeIYQY3pFyPqrOMaO4IYSLx-WNZNt0tMVn1bOcvyGEW9Gxp9UZ5rRDLerOK_8eAqQfenEx1C7U05pcgNrqvDTLnVvMWKRsPFzWeYypiJCmetsgDs5oX5tRhwFyrUNfLzDNMRUR7ucEOR9j5xSt8y4Mz6snVvsML7b7ovp6_eH26lNz8-Xj56t3N41hmCwNN73sJBWSayosN8Iyw4DvGZVMtIbuUd9KzCxYzhnprCEg9rbX0lpieYvpRfX6lFsmf18hL2py2YD3OkBcs-JCtEwQ-V8jllxKRlkxXp6MJsWcE1g1JzfpdFAYqWMp6kEp6lcp6lhKgV9uU9b9BP0fdGuhGMjJMLphvHMJ1O8PPvwVTJjCiqMCvf03dL16fwv3y0N6g9XcW_oTX-C7jg</recordid><startdate>20060313</startdate><enddate>20060313</enddate><creator>Raffaello, Anna</creator><creator>Laveder, Paolo</creator><creator>Romualdi, Chiara</creator><creator>Bean, Camilla</creator><creator>Toniolo, Luana</creator><creator>Germinario, Elena</creator><creator>Megighian, Aram</creator><creator>Danieli-Betto, Daniela</creator><creator>Reggiani, Carlo</creator><creator>Lanfranchi, Gerolamo</creator><general>Am Physiological Soc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20060313</creationdate><title>Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling</title><author>Raffaello, Anna ; Laveder, Paolo ; Romualdi, Chiara ; Bean, Camilla ; Toniolo, Luana ; Germinario, Elena ; Megighian, Aram ; Danieli-Betto, Daniela ; Reggiani, Carlo ; Lanfranchi, Gerolamo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-6cd9893796a37f6c7f5c5e6b539574c3b0d4915fef66528fc2e7bfda9ff2f6413</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Animals</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation</topic><topic>Mice</topic><topic>Mitochondria, Muscle - metabolism</topic><topic>Mitochondrial Proteins - genetics</topic><topic>Mitochondrial Proteins - metabolism</topic><topic>Muscle Denervation</topic><topic>Muscle Fibers, Fast-Twitch - metabolism</topic><topic>Muscle Proteins - genetics</topic><topic>Muscle Proteins - metabolism</topic><topic>Muscle, Skeletal - innervation</topic><topic>Muscle, Skeletal - metabolism</topic><topic>Muscular Atrophy - metabolism</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Reproducibility of Results</topic><topic>RNA, Messenger - metabolism</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Raffaello, Anna</creatorcontrib><creatorcontrib>Laveder, Paolo</creatorcontrib><creatorcontrib>Romualdi, Chiara</creatorcontrib><creatorcontrib>Bean, Camilla</creatorcontrib><creatorcontrib>Toniolo, Luana</creatorcontrib><creatorcontrib>Germinario, Elena</creatorcontrib><creatorcontrib>Megighian, Aram</creatorcontrib><creatorcontrib>Danieli-Betto, Daniela</creatorcontrib><creatorcontrib>Reggiani, Carlo</creatorcontrib><creatorcontrib>Lanfranchi, Gerolamo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiological genomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Raffaello, Anna</au><au>Laveder, Paolo</au><au>Romualdi, Chiara</au><au>Bean, Camilla</au><au>Toniolo, Luana</au><au>Germinario, Elena</au><au>Megighian, Aram</au><au>Danieli-Betto, Daniela</au><au>Reggiani, Carlo</au><au>Lanfranchi, Gerolamo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling</atitle><jtitle>Physiological genomics</jtitle><addtitle>Physiol Genomics</addtitle><date>2006-03-13</date><risdate>2006</risdate><volume>25</volume><issue>1</issue><spage>60</spage><epage>74</epage><pages>60-74</pages><issn>1094-8341</issn><eissn>1531-2267</eissn><abstract>1 Centro di Ricerca Interdipartimentale per le Biotecnologie Innovative (CRIBI) Biotechnology Center, University of Padova, Padua, Italy
2 Department of Anatomy and Physiology, University of Padova, Padua, Italy
Denervation deeply affects muscle structure and function, the alterations being different in slow and fast muscles. Because the effects of denervation on fast muscles are still controversial, and high-throughput studies on gene expression in denervated muscles are lacking, we studied gene expression during atrophy progression following denervation in mouse tibialis anterior (TA). The sciatic nerve was cut close to trochanter in adult CD1 mice. One, three, seven, and fourteen days after denervation, animals were killed and TA muscles were dissected out and utilized for physiological experiments and gene expression studies. Target cDNAs from TA muscles were hybridized on a dedicated cDNA microarray of muscle genes. Seventy-one genes were found differentially expressed. Microarray results were validated, and the expression of relevant genes not probed on our array was monitored by real-time quantitative PCR (RQ-PCR). Nuclear- and mitochondrial-encoded genes implicated in energy metabolism were consistently downregulated. Among genes implicated in muscle contraction (myofibrillar and sarcoplasmic reticulum), genes typical of fast fibers were downregulated, whereas those typical of slow fibers were upregulated. Electrophoresis and Western blot showed less pronounced changes in myofibrillar protein expression, partially confirming changes in gene expression. Isometric tension of skinned fibers was little affected by denervation, whereas calcium sensitivity decreased. Functional studies in mouse extensor digitorum longus muscle showed prolongation in twitch time parameters and shift to the left in force-frequency curves after denervation. We conclude that, if studied at the mRNA level, fast muscles appear not less responsive than slow muscles to the interruption of neural stimulation.
denervation; cDNA microarray; mitochondria; myosin</abstract><cop>United States</cop><pub>Am Physiological Soc</pub><pmid>16380408</pmid><doi>10.1152/physiolgenomics.00051.2005</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1094-8341 |
| ispartof | Physiological genomics, 2006-03, Vol.25 (1), p.60-74 |
| issn | 1094-8341 1531-2267 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19699535 |
| source | MEDLINE; American Physiological Society Paid; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Gene Expression Profiling Gene Expression Regulation Mice Mitochondria, Muscle - metabolism Mitochondrial Proteins - genetics Mitochondrial Proteins - metabolism Muscle Denervation Muscle Fibers, Fast-Twitch - metabolism Muscle Proteins - genetics Muscle Proteins - metabolism Muscle, Skeletal - innervation Muscle, Skeletal - metabolism Muscular Atrophy - metabolism Oligonucleotide Array Sequence Analysis Reproducibility of Results RNA, Messenger - metabolism Time Factors |
| title | Denervation in murine fast-twitch muscle: short-term physiological changes and temporal expression profiling |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T00%3A50%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Denervation%20in%20murine%20fast-twitch%20muscle:%20short-term%20physiological%20changes%20and%20temporal%20expression%20profiling&rft.jtitle=Physiological%20genomics&rft.au=Raffaello,%20Anna&rft.date=2006-03-13&rft.volume=25&rft.issue=1&rft.spage=60&rft.epage=74&rft.pages=60-74&rft.issn=1094-8341&rft.eissn=1531-2267&rft_id=info:doi/10.1152/physiolgenomics.00051.2005&rft_dat=%3Cproquest_cross%3E67745729%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19699535&rft_id=info:pmid/16380408&rfr_iscdi=true |