Optogenetic Control of Endoplasmic Reticulum–Mitochondria Tethering
The organelle interface emerges as a dynamic platform for a variety of biological responses. However, their study has been limited by the lack of tools to manipulate their occurrence in live cells spatiotemporally. Here, we report the development of a genetically encoded light-inducible tethering (L...
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| Veröffentlicht in: | ACS synthetic biology 2018-01, Vol.7 (1), p.2-9 |
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| container_title | ACS synthetic biology |
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| creator | Shi, Fan Kawano, Fuun Park, Seon-hye Emily Komazaki, Shinji Hirabayashi, Yusuke Polleux, Franck Yazawa, Masayuki |
| description | The organelle interface emerges as a dynamic platform for a variety of biological responses. However, their study has been limited by the lack of tools to manipulate their occurrence in live cells spatiotemporally. Here, we report the development of a genetically encoded light-inducible tethering (LIT) system allowing the induction of contacts between endoplasmic reticulum (ER) and mitochondria, taking advantage of a pair of light-dependent heterodimerization called an iLID system. We demonstrate that the iLID-based LIT approach enables control of ER–mitochondria tethering with high spatiotemporal precision in various cell types including primary neurons, which will facilitate the functional study of ER–mitochondrial contacts. |
| doi_str_mv | 10.1021/acssynbio.7b00248 |
| format | Article |
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| language | eng |
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| subjects | Animals Dimerization Endoplasmic Reticulum - metabolism Endoplasmic Reticulum - ultrastructure HEK293 Cells Humans Light Mice Microscopy, Electron Microscopy, Fluorescence Mitochondria - genetics Mitochondria - metabolism Mitochondria - radiation effects NIH 3T3 Cells Optogenetics |
| title | Optogenetic Control of Endoplasmic Reticulum–Mitochondria Tethering |
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