CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis
Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is re-expressed at the invasion front of colorectal cancer. CEACAM1 expression at metastatic sites remains to be investigated. The current study aims to clarify the association between CEACAM1 expression and recurrence after hepatec...
Gespeichert in:
| Veröffentlicht in: | The Journal of surgical research 2017-12, Vol.220, p.353-362 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 362 |
|---|---|
| container_issue | |
| container_start_page | 353 |
| container_title | The Journal of surgical research |
| container_volume | 220 |
| creator | Yamaguchi, Shunsuke Yokoyama, Shozo Ueno, Masaki Hayami, Shinya Mitani, Yasuyuki Takeuchi, Akihiro Shively, John E. Yamaue, Hiroki |
| description | Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is re-expressed at the invasion front of colorectal cancer. CEACAM1 expression at metastatic sites remains to be investigated. The current study aims to clarify the association between CEACAM1 expression and recurrence after hepatectomy of colorectal liver metastasis and to address whether CEACAM1 induces tumor-initiating properties needed for growth at metastatic sites.
Immunohistochemical analyses for CEACAM1 were performed in 67 patients with liver metastasis of colorectal cancer who had undergone curative hepatectomy. The risk factors for postoperative recurrence were calculated based on a CEACAM1 cytoplasmic domain isoform at the primary tumor invasion front. To investigate the effects of CEACAM1 cytoplasmic isoforms on HT29 and HCT116 colorectal cancer cells, Western blotting for CD44 and CD133, flow cytometry for ALDH1 activity, and soft-agar colony formation assay were performed.
CEACAM1 long (CEACAM1-L) and short (CEACAM1-S) cytoplasmic domain isoforms are strongly expressed on cancer cells in the liver metastases. Enhanced CEACAM1-S expression in the state of CEACAM1-L dominance at the primary tumor invasion front was an independent factor for colorectal cancer recurrence after curative hepatectomy. CEACAM1-4S-transfected HT29 and HCT116 cells had significantly higher CD44 expression and ALDH1 activity and increased the growth in anchorage-independent condition.
High expression of CEACAM1-S at the primary lesion invasion front is associated with recurrence and prognosis of patients with colorectal liver metastasis after curative hepatectomy. The expression of CEACAM1-4S enhances the tumor-initiating property of colorectal cancer cells. |
| doi_str_mv | 10.1016/j.jss.2017.07.035 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1969933363</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022480417305024</els_id><sourcerecordid>1969933363</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-d2b21ca30b94fc7bce9364be1d90d3d7fd5ad7927363a758d2113aea856cc0b63</originalsourceid><addsrcrecordid>eNp9kMtKAzEUhoMotlYfwI1k6WbGXOYWXJVSL1Bxo7gMmeQMzTDT1GRa6duboepS-CGEfOfn5EPompKUElrctWkbQsoILVMSw_MTNKVE5ElVlPwUTQlhLMkqkk3QRQgtiXdR8nM0YYJWhBE-RR-L5Xwxf6HYBqxCcNqqAQz-ssMae9A772GjAatmAI_XsI2venD9ATfOY-06F6FBdbiz-wj0MKgQY8MlOmtUF-Dq55yh94fl2-IpWb0-Pi_mq0TznA-JYTWjWnFSi6zRZa1B8CKrgRpBDDdlY3JlSsFKXnBV5pVhlHIFqsoLrUld8Bm6PfZuvfvcQRhkb4OGrlMbcLsgqSiE4DyOR5QeUe1dCB4aufW2V_4gKZGjT9nK6FOOPiWJiRvO0M1P_a7uwfxN_AqMwP0RgPjJvQUvg7ajMmNHM9I4-0_9NznGhqg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1969933363</pqid></control><display><type>article</type><title>CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Yamaguchi, Shunsuke ; Yokoyama, Shozo ; Ueno, Masaki ; Hayami, Shinya ; Mitani, Yasuyuki ; Takeuchi, Akihiro ; Shively, John E. ; Yamaue, Hiroki</creator><creatorcontrib>Yamaguchi, Shunsuke ; Yokoyama, Shozo ; Ueno, Masaki ; Hayami, Shinya ; Mitani, Yasuyuki ; Takeuchi, Akihiro ; Shively, John E. ; Yamaue, Hiroki</creatorcontrib><description>Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is re-expressed at the invasion front of colorectal cancer. CEACAM1 expression at metastatic sites remains to be investigated. The current study aims to clarify the association between CEACAM1 expression and recurrence after hepatectomy of colorectal liver metastasis and to address whether CEACAM1 induces tumor-initiating properties needed for growth at metastatic sites.
Immunohistochemical analyses for CEACAM1 were performed in 67 patients with liver metastasis of colorectal cancer who had undergone curative hepatectomy. The risk factors for postoperative recurrence were calculated based on a CEACAM1 cytoplasmic domain isoform at the primary tumor invasion front. To investigate the effects of CEACAM1 cytoplasmic isoforms on HT29 and HCT116 colorectal cancer cells, Western blotting for CD44 and CD133, flow cytometry for ALDH1 activity, and soft-agar colony formation assay were performed.
CEACAM1 long (CEACAM1-L) and short (CEACAM1-S) cytoplasmic domain isoforms are strongly expressed on cancer cells in the liver metastases. Enhanced CEACAM1-S expression in the state of CEACAM1-L dominance at the primary tumor invasion front was an independent factor for colorectal cancer recurrence after curative hepatectomy. CEACAM1-4S-transfected HT29 and HCT116 cells had significantly higher CD44 expression and ALDH1 activity and increased the growth in anchorage-independent condition.
High expression of CEACAM1-S at the primary lesion invasion front is associated with recurrence and prognosis of patients with colorectal liver metastasis after curative hepatectomy. The expression of CEACAM1-4S enhances the tumor-initiating property of colorectal cancer cells.</description><identifier>ISSN: 0022-4804</identifier><identifier>EISSN: 1095-8673</identifier><identifier>DOI: 10.1016/j.jss.2017.07.035</identifier><identifier>PMID: 29180203</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Aged ; ALDH activity ; Antigens, CD - metabolism ; Biomarkers - metabolism ; Cancer stem cell marker ; CD44 ; CEACAM1 ; Cell Adhesion Molecules - metabolism ; Colorectal cancer ; Colorectal Neoplasms - metabolism ; Colorectal Neoplasms - pathology ; Colorectal Neoplasms - surgery ; Female ; HCT116 Cells ; Hepatectomy ; HT29 Cells ; Humans ; Liver - pathology ; Liver metastasis ; Liver Neoplasms - metabolism ; Liver Neoplasms - secondary ; Male ; Neoplasm Recurrence, Local - metabolism ; Neoplasm Recurrence, Local - secondary ; Neoplastic Stem Cells - metabolism ; Protein Isoforms - metabolism ; Retrospective Studies</subject><ispartof>The Journal of surgical research, 2017-12, Vol.220, p.353-362</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-d2b21ca30b94fc7bce9364be1d90d3d7fd5ad7927363a758d2113aea856cc0b63</citedby><cites>FETCH-LOGICAL-c353t-d2b21ca30b94fc7bce9364be1d90d3d7fd5ad7927363a758d2113aea856cc0b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022480417305024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29180203$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yamaguchi, Shunsuke</creatorcontrib><creatorcontrib>Yokoyama, Shozo</creatorcontrib><creatorcontrib>Ueno, Masaki</creatorcontrib><creatorcontrib>Hayami, Shinya</creatorcontrib><creatorcontrib>Mitani, Yasuyuki</creatorcontrib><creatorcontrib>Takeuchi, Akihiro</creatorcontrib><creatorcontrib>Shively, John E.</creatorcontrib><creatorcontrib>Yamaue, Hiroki</creatorcontrib><title>CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis</title><title>The Journal of surgical research</title><addtitle>J Surg Res</addtitle><description>Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is re-expressed at the invasion front of colorectal cancer. CEACAM1 expression at metastatic sites remains to be investigated. The current study aims to clarify the association between CEACAM1 expression and recurrence after hepatectomy of colorectal liver metastasis and to address whether CEACAM1 induces tumor-initiating properties needed for growth at metastatic sites.
Immunohistochemical analyses for CEACAM1 were performed in 67 patients with liver metastasis of colorectal cancer who had undergone curative hepatectomy. The risk factors for postoperative recurrence were calculated based on a CEACAM1 cytoplasmic domain isoform at the primary tumor invasion front. To investigate the effects of CEACAM1 cytoplasmic isoforms on HT29 and HCT116 colorectal cancer cells, Western blotting for CD44 and CD133, flow cytometry for ALDH1 activity, and soft-agar colony formation assay were performed.
CEACAM1 long (CEACAM1-L) and short (CEACAM1-S) cytoplasmic domain isoforms are strongly expressed on cancer cells in the liver metastases. Enhanced CEACAM1-S expression in the state of CEACAM1-L dominance at the primary tumor invasion front was an independent factor for colorectal cancer recurrence after curative hepatectomy. CEACAM1-4S-transfected HT29 and HCT116 cells had significantly higher CD44 expression and ALDH1 activity and increased the growth in anchorage-independent condition.
High expression of CEACAM1-S at the primary lesion invasion front is associated with recurrence and prognosis of patients with colorectal liver metastasis after curative hepatectomy. The expression of CEACAM1-4S enhances the tumor-initiating property of colorectal cancer cells.</description><subject>Aged</subject><subject>ALDH activity</subject><subject>Antigens, CD - metabolism</subject><subject>Biomarkers - metabolism</subject><subject>Cancer stem cell marker</subject><subject>CD44</subject><subject>CEACAM1</subject><subject>Cell Adhesion Molecules - metabolism</subject><subject>Colorectal cancer</subject><subject>Colorectal Neoplasms - metabolism</subject><subject>Colorectal Neoplasms - pathology</subject><subject>Colorectal Neoplasms - surgery</subject><subject>Female</subject><subject>HCT116 Cells</subject><subject>Hepatectomy</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Liver - pathology</subject><subject>Liver metastasis</subject><subject>Liver Neoplasms - metabolism</subject><subject>Liver Neoplasms - secondary</subject><subject>Male</subject><subject>Neoplasm Recurrence, Local - metabolism</subject><subject>Neoplasm Recurrence, Local - secondary</subject><subject>Neoplastic Stem Cells - metabolism</subject><subject>Protein Isoforms - metabolism</subject><subject>Retrospective Studies</subject><issn>0022-4804</issn><issn>1095-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotlYfwI1k6WbGXOYWXJVSL1Bxo7gMmeQMzTDT1GRa6duboepS-CGEfOfn5EPompKUElrctWkbQsoILVMSw_MTNKVE5ElVlPwUTQlhLMkqkk3QRQgtiXdR8nM0YYJWhBE-RR-L5Xwxf6HYBqxCcNqqAQz-ssMae9A772GjAatmAI_XsI2venD9ATfOY-06F6FBdbiz-wj0MKgQY8MlOmtUF-Dq55yh94fl2-IpWb0-Pi_mq0TznA-JYTWjWnFSi6zRZa1B8CKrgRpBDDdlY3JlSsFKXnBV5pVhlHIFqsoLrUld8Bm6PfZuvfvcQRhkb4OGrlMbcLsgqSiE4DyOR5QeUe1dCB4aufW2V_4gKZGjT9nK6FOOPiWJiRvO0M1P_a7uwfxN_AqMwP0RgPjJvQUvg7ajMmNHM9I4-0_9NznGhqg</recordid><startdate>201712</startdate><enddate>201712</enddate><creator>Yamaguchi, Shunsuke</creator><creator>Yokoyama, Shozo</creator><creator>Ueno, Masaki</creator><creator>Hayami, Shinya</creator><creator>Mitani, Yasuyuki</creator><creator>Takeuchi, Akihiro</creator><creator>Shively, John E.</creator><creator>Yamaue, Hiroki</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201712</creationdate><title>CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis</title><author>Yamaguchi, Shunsuke ; Yokoyama, Shozo ; Ueno, Masaki ; Hayami, Shinya ; Mitani, Yasuyuki ; Takeuchi, Akihiro ; Shively, John E. ; Yamaue, Hiroki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-d2b21ca30b94fc7bce9364be1d90d3d7fd5ad7927363a758d2113aea856cc0b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aged</topic><topic>ALDH activity</topic><topic>Antigens, CD - metabolism</topic><topic>Biomarkers - metabolism</topic><topic>Cancer stem cell marker</topic><topic>CD44</topic><topic>CEACAM1</topic><topic>Cell Adhesion Molecules - metabolism</topic><topic>Colorectal cancer</topic><topic>Colorectal Neoplasms - metabolism</topic><topic>Colorectal Neoplasms - pathology</topic><topic>Colorectal Neoplasms - surgery</topic><topic>Female</topic><topic>HCT116 Cells</topic><topic>Hepatectomy</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Liver - pathology</topic><topic>Liver metastasis</topic><topic>Liver Neoplasms - metabolism</topic><topic>Liver Neoplasms - secondary</topic><topic>Male</topic><topic>Neoplasm Recurrence, Local - metabolism</topic><topic>Neoplasm Recurrence, Local - secondary</topic><topic>Neoplastic Stem Cells - metabolism</topic><topic>Protein Isoforms - metabolism</topic><topic>Retrospective Studies</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yamaguchi, Shunsuke</creatorcontrib><creatorcontrib>Yokoyama, Shozo</creatorcontrib><creatorcontrib>Ueno, Masaki</creatorcontrib><creatorcontrib>Hayami, Shinya</creatorcontrib><creatorcontrib>Mitani, Yasuyuki</creatorcontrib><creatorcontrib>Takeuchi, Akihiro</creatorcontrib><creatorcontrib>Shively, John E.</creatorcontrib><creatorcontrib>Yamaue, Hiroki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of surgical research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yamaguchi, Shunsuke</au><au>Yokoyama, Shozo</au><au>Ueno, Masaki</au><au>Hayami, Shinya</au><au>Mitani, Yasuyuki</au><au>Takeuchi, Akihiro</au><au>Shively, John E.</au><au>Yamaue, Hiroki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis</atitle><jtitle>The Journal of surgical research</jtitle><addtitle>J Surg Res</addtitle><date>2017-12</date><risdate>2017</risdate><volume>220</volume><spage>353</spage><epage>362</epage><pages>353-362</pages><issn>0022-4804</issn><eissn>1095-8673</eissn><abstract>Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is re-expressed at the invasion front of colorectal cancer. CEACAM1 expression at metastatic sites remains to be investigated. The current study aims to clarify the association between CEACAM1 expression and recurrence after hepatectomy of colorectal liver metastasis and to address whether CEACAM1 induces tumor-initiating properties needed for growth at metastatic sites.
Immunohistochemical analyses for CEACAM1 were performed in 67 patients with liver metastasis of colorectal cancer who had undergone curative hepatectomy. The risk factors for postoperative recurrence were calculated based on a CEACAM1 cytoplasmic domain isoform at the primary tumor invasion front. To investigate the effects of CEACAM1 cytoplasmic isoforms on HT29 and HCT116 colorectal cancer cells, Western blotting for CD44 and CD133, flow cytometry for ALDH1 activity, and soft-agar colony formation assay were performed.
CEACAM1 long (CEACAM1-L) and short (CEACAM1-S) cytoplasmic domain isoforms are strongly expressed on cancer cells in the liver metastases. Enhanced CEACAM1-S expression in the state of CEACAM1-L dominance at the primary tumor invasion front was an independent factor for colorectal cancer recurrence after curative hepatectomy. CEACAM1-4S-transfected HT29 and HCT116 cells had significantly higher CD44 expression and ALDH1 activity and increased the growth in anchorage-independent condition.
High expression of CEACAM1-S at the primary lesion invasion front is associated with recurrence and prognosis of patients with colorectal liver metastasis after curative hepatectomy. The expression of CEACAM1-4S enhances the tumor-initiating property of colorectal cancer cells.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>29180203</pmid><doi>10.1016/j.jss.2017.07.035</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-4804 |
| ispartof | The Journal of surgical research, 2017-12, Vol.220, p.353-362 |
| issn | 0022-4804 1095-8673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1969933363 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Aged ALDH activity Antigens, CD - metabolism Biomarkers - metabolism Cancer stem cell marker CD44 CEACAM1 Cell Adhesion Molecules - metabolism Colorectal cancer Colorectal Neoplasms - metabolism Colorectal Neoplasms - pathology Colorectal Neoplasms - surgery Female HCT116 Cells Hepatectomy HT29 Cells Humans Liver - pathology Liver metastasis Liver Neoplasms - metabolism Liver Neoplasms - secondary Male Neoplasm Recurrence, Local - metabolism Neoplasm Recurrence, Local - secondary Neoplastic Stem Cells - metabolism Protein Isoforms - metabolism Retrospective Studies |
| title | CEACAM1 is associated with recurrence after hepatectomy for colorectal liver metastasis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-24T23%3A35%3A08IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=CEACAM1%20is%20associated%20with%20recurrence%20after%20hepatectomy%20for%20colorectal%20liver%20metastasis&rft.jtitle=The%20Journal%20of%20surgical%20research&rft.au=Yamaguchi,%20Shunsuke&rft.date=2017-12&rft.volume=220&rft.spage=353&rft.epage=362&rft.pages=353-362&rft.issn=0022-4804&rft.eissn=1095-8673&rft_id=info:doi/10.1016/j.jss.2017.07.035&rft_dat=%3Cproquest_cross%3E1969933363%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1969933363&rft_id=info:pmid/29180203&rft_els_id=S0022480417305024&rfr_iscdi=true |