A novel screen-printed mast cell-based electrochemical sensor for detecting spoilage bacterial quorum signaling molecules (N-acyl-homoserine-lactones) in freshwater fish
A novel screen-printed cell-based electrochemical sensor was developed to assess bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs). Screen-printed carbon electrode (SPCE), which possesses excellent properties such as low-cost, disposable and energy-efficient, was modified with m...
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| Veröffentlicht in: | Biosensors & bioelectronics 2018-04, Vol.102, p.396-402 |
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| creator | Jiang, Donglei Liu, Yan Jiang, Hui Rao, Shengqi Fang, Wu Wu, Mangang Yuan, Limin Fang, Weiming |
| description | A novel screen-printed cell-based electrochemical sensor was developed to assess bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs). Screen-printed carbon electrode (SPCE), which possesses excellent properties such as low-cost, disposable and energy-efficient, was modified with multi-walled carbon nanotubes (MWNTs) to improve electrochemical signals and enhance the sensitivity. Rat basophilic leukemia (RBL-2H3) mast cells encapsulated in alginate/graphene oxide (NaAgl/GO) hydrogel were immobilized on the MWNTs/SPCE to serve as recognition element. Electrochemical impedance spectroscopy (EIS) was employed to record the cell impedance signal as-influenced by Pseudomonas aeruginosa quorum-sensing molecule, N−3-oxododecanoyl homoserine lactone (3OC12-HSL). Experimental results show that 3OC12-HSL caused a significant decrease in cell viability in a dose dependent manner. The EIS value decreased with concentrations of 3OC12-HSL in the range of 0.1–1μM, and the detection limit for 3OC12-HSL was calculated to be 0.094μM. These results were confirmed via cell viability, SEM, TEM analysis. Next, the sensor was successfully applied to monitoring the production of AHLs by spoilage bacteria in three different freshwater fish juice samples which efficiently proved the practicability of this cell based method. Therefore, the proposed cell sensor may serve as an innovative and effective approach to the measurement of quorum signaling molecule and thus provides a new avenue for real-time monitoring the spoilage bacteria in freshwater fish production.
•A novel RBL-2H3 cell electrochemical sensor was developed for detecting spoilage bacterial AHLs in freshwater fish.•The low-cost, disposable SPCE greatly improved the stability and repeatability of the cell sensor.•This study provides a new way to monitor the contamination of spoilage bacterial in freshwater fish products. |
| doi_str_mv | 10.1016/j.bios.2017.11.040 |
| format | Article |
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•A novel RBL-2H3 cell electrochemical sensor was developed for detecting spoilage bacterial AHLs in freshwater fish.•The low-cost, disposable SPCE greatly improved the stability and repeatability of the cell sensor.•This study provides a new way to monitor the contamination of spoilage bacterial in freshwater fish products.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2017.11.040</identifier><identifier>PMID: 29174973</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - chemistry ; 4-Butyrolactone - isolation & purification ; Animals ; Biosensing Techniques ; Cell based electrochemical sensor ; Dielectric Spectroscopy ; Fishes - microbiology ; Fresh Water - microbiology ; Freshwater fish ; Graphite - chemistry ; Homoserine - analogs & derivatives ; Homoserine - chemistry ; Homoserine - isolation & purification ; Mast Cells - chemistry ; Nanotubes, Carbon - chemistry ; Pseudomonas aeruginosa - chemistry ; Pseudomonas aeruginosa - isolation & purification ; Quorum Sensing ; Quorum signaling molecules ; Rats ; Screen-printed carbon electrode ; Spoilage bacteria</subject><ispartof>Biosensors & bioelectronics, 2018-04, Vol.102, p.396-402</ispartof><rights>2017 Elsevier B.V.</rights><rights>Copyright © 2017 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-f5b3184c100cff094c1cf9cf2bc3b27bed2b8946c8e4d4c63b6578a847130de23</citedby><cites>FETCH-LOGICAL-c395t-f5b3184c100cff094c1cf9cf2bc3b27bed2b8946c8e4d4c63b6578a847130de23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0956566317307686$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29174973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Donglei</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Jiang, Hui</creatorcontrib><creatorcontrib>Rao, Shengqi</creatorcontrib><creatorcontrib>Fang, Wu</creatorcontrib><creatorcontrib>Wu, Mangang</creatorcontrib><creatorcontrib>Yuan, Limin</creatorcontrib><creatorcontrib>Fang, Weiming</creatorcontrib><title>A novel screen-printed mast cell-based electrochemical sensor for detecting spoilage bacterial quorum signaling molecules (N-acyl-homoserine-lactones) in freshwater fish</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>A novel screen-printed cell-based electrochemical sensor was developed to assess bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs). Screen-printed carbon electrode (SPCE), which possesses excellent properties such as low-cost, disposable and energy-efficient, was modified with multi-walled carbon nanotubes (MWNTs) to improve electrochemical signals and enhance the sensitivity. Rat basophilic leukemia (RBL-2H3) mast cells encapsulated in alginate/graphene oxide (NaAgl/GO) hydrogel were immobilized on the MWNTs/SPCE to serve as recognition element. Electrochemical impedance spectroscopy (EIS) was employed to record the cell impedance signal as-influenced by Pseudomonas aeruginosa quorum-sensing molecule, N−3-oxododecanoyl homoserine lactone (3OC12-HSL). Experimental results show that 3OC12-HSL caused a significant decrease in cell viability in a dose dependent manner. The EIS value decreased with concentrations of 3OC12-HSL in the range of 0.1–1μM, and the detection limit for 3OC12-HSL was calculated to be 0.094μM. These results were confirmed via cell viability, SEM, TEM analysis. Next, the sensor was successfully applied to monitoring the production of AHLs by spoilage bacteria in three different freshwater fish juice samples which efficiently proved the practicability of this cell based method. Therefore, the proposed cell sensor may serve as an innovative and effective approach to the measurement of quorum signaling molecule and thus provides a new avenue for real-time monitoring the spoilage bacteria in freshwater fish production.
•A novel RBL-2H3 cell electrochemical sensor was developed for detecting spoilage bacterial AHLs in freshwater fish.•The low-cost, disposable SPCE greatly improved the stability and repeatability of the cell sensor.•This study provides a new way to monitor the contamination of spoilage bacterial in freshwater fish products.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - chemistry</subject><subject>4-Butyrolactone - isolation & purification</subject><subject>Animals</subject><subject>Biosensing Techniques</subject><subject>Cell based electrochemical sensor</subject><subject>Dielectric Spectroscopy</subject><subject>Fishes - microbiology</subject><subject>Fresh Water - microbiology</subject><subject>Freshwater fish</subject><subject>Graphite - chemistry</subject><subject>Homoserine - analogs & derivatives</subject><subject>Homoserine - chemistry</subject><subject>Homoserine - isolation & purification</subject><subject>Mast Cells - chemistry</subject><subject>Nanotubes, Carbon - chemistry</subject><subject>Pseudomonas aeruginosa - chemistry</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Quorum Sensing</subject><subject>Quorum signaling molecules</subject><subject>Rats</subject><subject>Screen-printed carbon electrode</subject><subject>Spoilage bacteria</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc2OFCEUhYnROO3oC7gwLMcFJRT1R-JmMvEvmehG1wSoW910KOjhVs1kHsm3lEqPLl2QC7nfOQnnEPJW8Epw0X04VtYnrGou-kqIijf8GdmJoZesqWX7nOy4ajvWdp28IK8Qj5zzXij-klzUSvSN6uWO_L6mMd1DoOgyQGSn7OMCI50NLtRBCMwaLG8I4Jac3AFm70zBIWLKdCpnhKXsfNxTPCUfzB6oNW6B7At3t6a8zhT9PpqwMXMqTmsApFffmXGPgR3SnLDQEVgouhQB31Mf6ZQBDw-mGNHJ4-E1eTGZgPDmaV6SX58__bz5ym5_fPl2c33LnFTtwqbWSjE0TnDupomrcnOTclNtnbR1b2Gs7aCazg3QjI3rpO3afjBD0wvJR6jlJbk6-55yulsBFz173JIwEdKKWqhOKSkElwWtz6jLCTHDpEt8s8mPWnC9VaSPeqtIbxVpIXSpqIjePfmvdobxn-RvJwX4eAag_PLeQ9boPEQHo88laD0m_z__PxV8pzA</recordid><startdate>20180415</startdate><enddate>20180415</enddate><creator>Jiang, Donglei</creator><creator>Liu, Yan</creator><creator>Jiang, Hui</creator><creator>Rao, Shengqi</creator><creator>Fang, Wu</creator><creator>Wu, Mangang</creator><creator>Yuan, Limin</creator><creator>Fang, Weiming</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180415</creationdate><title>A novel screen-printed mast cell-based electrochemical sensor for detecting spoilage bacterial quorum signaling molecules (N-acyl-homoserine-lactones) in freshwater fish</title><author>Jiang, Donglei ; Liu, Yan ; Jiang, Hui ; Rao, Shengqi ; Fang, Wu ; Wu, Mangang ; Yuan, Limin ; Fang, Weiming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-f5b3184c100cff094c1cf9cf2bc3b27bed2b8946c8e4d4c63b6578a847130de23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - chemistry</topic><topic>4-Butyrolactone - isolation & purification</topic><topic>Animals</topic><topic>Biosensing Techniques</topic><topic>Cell based electrochemical sensor</topic><topic>Dielectric Spectroscopy</topic><topic>Fishes - microbiology</topic><topic>Fresh Water - microbiology</topic><topic>Freshwater fish</topic><topic>Graphite - chemistry</topic><topic>Homoserine - analogs & derivatives</topic><topic>Homoserine - chemistry</topic><topic>Homoserine - isolation & purification</topic><topic>Mast Cells - chemistry</topic><topic>Nanotubes, Carbon - chemistry</topic><topic>Pseudomonas aeruginosa - chemistry</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Quorum Sensing</topic><topic>Quorum signaling molecules</topic><topic>Rats</topic><topic>Screen-printed carbon electrode</topic><topic>Spoilage bacteria</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Donglei</creatorcontrib><creatorcontrib>Liu, Yan</creatorcontrib><creatorcontrib>Jiang, Hui</creatorcontrib><creatorcontrib>Rao, Shengqi</creatorcontrib><creatorcontrib>Fang, Wu</creatorcontrib><creatorcontrib>Wu, Mangang</creatorcontrib><creatorcontrib>Yuan, Limin</creatorcontrib><creatorcontrib>Fang, Weiming</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Donglei</au><au>Liu, Yan</au><au>Jiang, Hui</au><au>Rao, Shengqi</au><au>Fang, Wu</au><au>Wu, Mangang</au><au>Yuan, Limin</au><au>Fang, Weiming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel screen-printed mast cell-based electrochemical sensor for detecting spoilage bacterial quorum signaling molecules (N-acyl-homoserine-lactones) in freshwater fish</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2018-04-15</date><risdate>2018</risdate><volume>102</volume><spage>396</spage><epage>402</epage><pages>396-402</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>A novel screen-printed cell-based electrochemical sensor was developed to assess bacterial quorum signaling molecules, N-acylhomoserine lactones (AHLs). Screen-printed carbon electrode (SPCE), which possesses excellent properties such as low-cost, disposable and energy-efficient, was modified with multi-walled carbon nanotubes (MWNTs) to improve electrochemical signals and enhance the sensitivity. Rat basophilic leukemia (RBL-2H3) mast cells encapsulated in alginate/graphene oxide (NaAgl/GO) hydrogel were immobilized on the MWNTs/SPCE to serve as recognition element. Electrochemical impedance spectroscopy (EIS) was employed to record the cell impedance signal as-influenced by Pseudomonas aeruginosa quorum-sensing molecule, N−3-oxododecanoyl homoserine lactone (3OC12-HSL). Experimental results show that 3OC12-HSL caused a significant decrease in cell viability in a dose dependent manner. The EIS value decreased with concentrations of 3OC12-HSL in the range of 0.1–1μM, and the detection limit for 3OC12-HSL was calculated to be 0.094μM. These results were confirmed via cell viability, SEM, TEM analysis. Next, the sensor was successfully applied to monitoring the production of AHLs by spoilage bacteria in three different freshwater fish juice samples which efficiently proved the practicability of this cell based method. Therefore, the proposed cell sensor may serve as an innovative and effective approach to the measurement of quorum signaling molecule and thus provides a new avenue for real-time monitoring the spoilage bacteria in freshwater fish production.
•A novel RBL-2H3 cell electrochemical sensor was developed for detecting spoilage bacterial AHLs in freshwater fish.•The low-cost, disposable SPCE greatly improved the stability and repeatability of the cell sensor.•This study provides a new way to monitor the contamination of spoilage bacterial in freshwater fish products.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>29174973</pmid><doi>10.1016/j.bios.2017.11.040</doi><tpages>7</tpages></addata></record> |
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| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - chemistry 4-Butyrolactone - isolation & purification Animals Biosensing Techniques Cell based electrochemical sensor Dielectric Spectroscopy Fishes - microbiology Fresh Water - microbiology Freshwater fish Graphite - chemistry Homoserine - analogs & derivatives Homoserine - chemistry Homoserine - isolation & purification Mast Cells - chemistry Nanotubes, Carbon - chemistry Pseudomonas aeruginosa - chemistry Pseudomonas aeruginosa - isolation & purification Quorum Sensing Quorum signaling molecules Rats Screen-printed carbon electrode Spoilage bacteria |
| title | A novel screen-printed mast cell-based electrochemical sensor for detecting spoilage bacterial quorum signaling molecules (N-acyl-homoserine-lactones) in freshwater fish |
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