A new enrichment diagnostic platform for semen culture
Urogenital bacterial infections have been described in literature as a potential cause of infertility. For the consequences that a failure in diagnosis could have on the evolution of male urogenital infectious disease, an accurate microbiological procedure to investigate the bacterial species compos...
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| Veröffentlicht in: | Journal of microbiological methods 2018-01, Vol.144, p.168-172 |
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| container_end_page | 172 |
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| container_start_page | 168 |
| container_title | Journal of microbiological methods |
| container_volume | 144 |
| creator | Iovene, Maria Rosaria Martora, Francesca Bombace, Francesca Montella, Fortunato Del Vecchio, Chiara De Rosa, Michele D'Oriano, Virginia Galdiero, Marilena Vitiello, Mariateresa |
| description | Urogenital bacterial infections have been described in literature as a potential cause of infertility. For the consequences that a failure in diagnosis could have on the evolution of male urogenital infectious disease, an accurate microbiological procedure to investigate the bacterial species composition of seminal fluid plays a crucial role to better understand the eventual correlation with infertility. In order to improve the quality of semen culture investigations, we have developed a new enrichment diagnostic platform. Semen samples of 540 infertile men were simultaneously analyzed using the standard microbiological semen culture method and an alternative new experimental technique (Brain Heart Infusion broth, BHI, enrichment). Our results established the possibility to apply BHI enrichment to detect bacteria from semen samples with higher sensitivity (100%) and negative predictive value (100%) than the standard technique.
•Urogenital bacterial infections are a potential cause of infertility.•A new enrichment diagnostic platform to improve the quality of semen culture investigations•BHI enrichment to detect bacteria from semen samples |
| doi_str_mv | 10.1016/j.mimet.2017.11.018 |
| format | Article |
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| identifier | ISSN: 0167-7012 |
| ispartof | Journal of microbiological methods, 2018-01, Vol.144, p.168-172 |
| issn | 0167-7012 1872-8359 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | BHI enrichment Infertility Semen culture |
| title | A new enrichment diagnostic platform for semen culture |
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