Development of a multireactor microfluidic system for the determination of DNA using real-time polymerase chain reaction

Development of a multireactor microfluidic system for the determination of DNA using real-time polymerase chain reaction

A microfluidic system that allowed us to perform the real-time polymerase chain reaction (PCR) in a glass-silicon microchip containing nine 250-nL microreactors was developed and studied. The resulting high heating/cooling rates of a PCR mixture in a microreactor allowed us to optimize the amplifica...

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Veröffentlicht in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2008-02, Vol.63 (2), p.192-198
Hauptverfasser: Slyadnev, M N, Lavrova, M V, Erkin, MA, Kazakov, V A, Ganeev, A A
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container_title Journal of analytical chemistry (New York, N.Y.)
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creator Slyadnev, M N
Lavrova, M V
Erkin, MA
Kazakov, V A
Ganeev, A A
description A microfluidic system that allowed us to perform the real-time polymerase chain reaction (PCR) in a glass-silicon microchip containing nine 250-nL microreactors was developed and studied. The resulting high heating/cooling rates of a PCR mixture in a microreactor allowed us to optimize the amplification mode (1 min/cycle). The silicon surface of microreactors was successfully passivated. The resulting analytical system allowed us to measure the PCR kinetic curves in chip microreactors at a DNA concentration of similar to 5 x 10 super(4) copies per microreactor. It was found that, if the PCR is performed in a microchip with real-time detection using the optimized amplification mode, the result can be obtained 13-14 min after the onset of reaction.
doi_str_mv 10.1007/s10809-008-2015-2
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title Development of a multireactor microfluidic system for the determination of DNA using real-time polymerase chain reaction
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