High production of laccase B from Trametes sp. in Pichia pastoris

The coding sequence of a laccase isozyme from Trametes sp. AH28-2 was cloned in pPIC9K vector and heterologously overexpressed in the yeast Pichia pastoris strain GS115. In the minimal medium containing 0.3 mM CuSO₄ and 0.6% alanine, the maximum yield of the recombinant laccase rLacB reached 32,000...

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Veröffentlicht in:	World journal of microbiology & biotechnology 2007-05, Vol.23 (5), p.741-745
Hauptverfasser:	Li, J. F, Hong, Y. Z, Xiao, Y. Z, Xu, Y. H, Fang, W
Format:	Artikel
Sprache:	eng
Schlagworte:	Alanine Biological and medical sciences Biotechnology Decoloring Decolorization Dye decolorization Enzymes Fundamental and applied biological sciences. Psychology Fungal laccase Heterologous expression Laccase Microbiology Pichia pastoris Thermal stability Trametes Yeasts
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