Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment
Recent molecular evidence suggests that different species and/or genotypes of Cryptosporidium display strong host specificity, altering our perceptions regarding the zoonotic potential of this parasite. Molecular forensic profiling of the small-subunit rRNA gene from oocysts enumerated on microscope...
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 2007-06, Vol.73 (12), p.3945-3957 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3957 |
|---|---|
| container_issue | 12 |
| container_start_page | 3945 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 73 |
| creator | Ruecker, Norma J Braithwaite, Shannon L Topp, Edward Edge, Thomas Lapen, David R Wilkes, Graham Robertson, Will Medeiros, Diane Sensen, Christoph W Neumann, Norman F |
| description | Recent molecular evidence suggests that different species and/or genotypes of Cryptosporidium display strong host specificity, altering our perceptions regarding the zoonotic potential of this parasite. Molecular forensic profiling of the small-subunit rRNA gene from oocysts enumerated on microscope slides by U.S. Environmental Protection Agency method 1623 was used to identify the range and prevalence of Cryptosporidium species and genotypes in the South Nation watershed in Ontario, Canada. Fourteen sites within the watershed were monitored weekly for 10 weeks to assess the occurrence, molecular composition, and host sources of Cryptosporidium parasites impacting water within the region. Cryptosporidium andersoni, Cryptosporidium muskrat genotype II, Cryptosporidium cervine genotype, C. baileyi, C. parvum, Cryptosporidium muskrat genotype I, the Cryptosporidium fox genotype, genotype W1, and genotype W12 were detected in the watershed. The molecular composition of the Cryptosporidium parasites, supported by general land use analysis, indicated that mature cattle were likely the main source of contamination of the watershed. Deer, muskrats, voles, birds, and other wildlife species, in addition to sewage (human or agricultural) may also potentially impact water quality within the study area. Source water protection studies that use land use analysis with molecular genotyping of Cryptosporidium parasites may provide a more robust source-tracking tool to characterize fecal impacts in a watershed. Moreover, the information is vital for assessing environmental and human health risks posed by water contaminated with zoonotic and/or anthroponotic forms of CRYPTOSPORIDIUM: |
| doi_str_mv | 10.1128/AEM.02788-06 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_highw</sourceid><recordid>TN_cdi_proquest_miscellaneous_19679729</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>70612687</sourcerecordid><originalsourceid>FETCH-LOGICAL-c521t-cc158fd72151526a2d38d973dcf6bda047e83e3f9c0e7196cacf75f0759926813</originalsourceid><addsrcrecordid>eNqFkU1vEzEYhFcIREPhxhksJDix4bW9_rogRVEhlYqQ-iFuWK7XTtzurhd706r_HkMiWrhw8uF9NDOeqaqXGOYYE_lhcfRlDkRIWQN_VM0wKFkzSvnjagagVE1IAwfVs5yvAKABLp9WB1g0khLBZ9X382TsdRjWaBXzhM7iNlmXUfRome7GKeYxptCGbY_yOM5RGNCpuUXfzOQS8jGh435M8ca1aOVMN23QacjXaJGzy7l3w_S8euJNl92L_XtYXXw6Ol-u6pOvn4-Xi5PaMoKn2lrMpG8FwQwzwg1pqWyVoK31_LI10AgnqaNeWXACK26N9YJ5EEwpwiWmh9XHne64vexda4t1Mp0eU-hNutPRBP33ZQgbvY43GqvSA8gi8G4vkOKPrcuT7kO2ruvM4OI2awEcFyfxX7CkE0oQVcA3_4BXpdyhtKAJMMUVw6RA73eQTTHn5PyfyBj0r3l1mVf_nlcDL_irh9-8h_d7FuDtHjDZms4nM9iQ7zkpi3PzINwmrDe3ITltcq-N67WgGhNNVcMK9HoHeRO1WacidHFGAFMAIalkDf0JRd3Bkw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>205969512</pqid></control><display><type>article</type><title>Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment</title><source>MEDLINE</source><source>American Society for Microbiology Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Ruecker, Norma J ; Braithwaite, Shannon L ; Topp, Edward ; Edge, Thomas ; Lapen, David R ; Wilkes, Graham ; Robertson, Will ; Medeiros, Diane ; Sensen, Christoph W ; Neumann, Norman F</creator><creatorcontrib>Ruecker, Norma J ; Braithwaite, Shannon L ; Topp, Edward ; Edge, Thomas ; Lapen, David R ; Wilkes, Graham ; Robertson, Will ; Medeiros, Diane ; Sensen, Christoph W ; Neumann, Norman F</creatorcontrib><description>Recent molecular evidence suggests that different species and/or genotypes of Cryptosporidium display strong host specificity, altering our perceptions regarding the zoonotic potential of this parasite. Molecular forensic profiling of the small-subunit rRNA gene from oocysts enumerated on microscope slides by U.S. Environmental Protection Agency method 1623 was used to identify the range and prevalence of Cryptosporidium species and genotypes in the South Nation watershed in Ontario, Canada. Fourteen sites within the watershed were monitored weekly for 10 weeks to assess the occurrence, molecular composition, and host sources of Cryptosporidium parasites impacting water within the region. Cryptosporidium andersoni, Cryptosporidium muskrat genotype II, Cryptosporidium cervine genotype, C. baileyi, C. parvum, Cryptosporidium muskrat genotype I, the Cryptosporidium fox genotype, genotype W1, and genotype W12 were detected in the watershed. The molecular composition of the Cryptosporidium parasites, supported by general land use analysis, indicated that mature cattle were likely the main source of contamination of the watershed. Deer, muskrats, voles, birds, and other wildlife species, in addition to sewage (human or agricultural) may also potentially impact water quality within the study area. Source water protection studies that use land use analysis with molecular genotyping of Cryptosporidium parasites may provide a more robust source-tracking tool to characterize fecal impacts in a watershed. Moreover, the information is vital for assessing environmental and human health risks posed by water contaminated with zoonotic and/or anthroponotic forms of CRYPTOSPORIDIUM:</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>DOI: 10.1128/AEM.02788-06</identifier><identifier>PMID: 17483276</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Baileyi ; Base Sequence ; Biological and medical sciences ; Cluster Analysis ; Cryptosporidium ; Cryptosporidium - genetics ; Cryptosporidium andersoni ; Feces - parasitology ; Freshwater ; Fundamental and applied biological sciences. Psychology ; Genetic Variation ; Genotype ; Genotype & phenotype ; Health risk assessment ; Microbiology ; Molecular Sequence Data ; Ontario ; Parasites ; Phylogeny ; Public Health Microbiology ; Ribotyping ; Rivers - parasitology ; RNA, Ribosomal - genetics ; Sequence Analysis, DNA ; Species Specificity ; Watersheds</subject><ispartof>Applied and Environmental Microbiology, 2007-06, Vol.73 (12), p.3945-3957</ispartof><rights>2007 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Jun 2007</rights><rights>Copyright © 2007, American Society for Microbiology 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-cc158fd72151526a2d38d973dcf6bda047e83e3f9c0e7196cacf75f0759926813</citedby><cites>FETCH-LOGICAL-c521t-cc158fd72151526a2d38d973dcf6bda047e83e3f9c0e7196cacf75f0759926813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932708/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1932708/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18859649$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17483276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ruecker, Norma J</creatorcontrib><creatorcontrib>Braithwaite, Shannon L</creatorcontrib><creatorcontrib>Topp, Edward</creatorcontrib><creatorcontrib>Edge, Thomas</creatorcontrib><creatorcontrib>Lapen, David R</creatorcontrib><creatorcontrib>Wilkes, Graham</creatorcontrib><creatorcontrib>Robertson, Will</creatorcontrib><creatorcontrib>Medeiros, Diane</creatorcontrib><creatorcontrib>Sensen, Christoph W</creatorcontrib><creatorcontrib>Neumann, Norman F</creatorcontrib><title>Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Recent molecular evidence suggests that different species and/or genotypes of Cryptosporidium display strong host specificity, altering our perceptions regarding the zoonotic potential of this parasite. Molecular forensic profiling of the small-subunit rRNA gene from oocysts enumerated on microscope slides by U.S. Environmental Protection Agency method 1623 was used to identify the range and prevalence of Cryptosporidium species and genotypes in the South Nation watershed in Ontario, Canada. Fourteen sites within the watershed were monitored weekly for 10 weeks to assess the occurrence, molecular composition, and host sources of Cryptosporidium parasites impacting water within the region. Cryptosporidium andersoni, Cryptosporidium muskrat genotype II, Cryptosporidium cervine genotype, C. baileyi, C. parvum, Cryptosporidium muskrat genotype I, the Cryptosporidium fox genotype, genotype W1, and genotype W12 were detected in the watershed. The molecular composition of the Cryptosporidium parasites, supported by general land use analysis, indicated that mature cattle were likely the main source of contamination of the watershed. Deer, muskrats, voles, birds, and other wildlife species, in addition to sewage (human or agricultural) may also potentially impact water quality within the study area. Source water protection studies that use land use analysis with molecular genotyping of Cryptosporidium parasites may provide a more robust source-tracking tool to characterize fecal impacts in a watershed. Moreover, the information is vital for assessing environmental and human health risks posed by water contaminated with zoonotic and/or anthroponotic forms of CRYPTOSPORIDIUM:</description><subject>Animals</subject><subject>Baileyi</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cluster Analysis</subject><subject>Cryptosporidium</subject><subject>Cryptosporidium - genetics</subject><subject>Cryptosporidium andersoni</subject><subject>Feces - parasitology</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Genotype & phenotype</subject><subject>Health risk assessment</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Ontario</subject><subject>Parasites</subject><subject>Phylogeny</subject><subject>Public Health Microbiology</subject><subject>Ribotyping</subject><subject>Rivers - parasitology</subject><subject>RNA, Ribosomal - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Species Specificity</subject><subject>Watersheds</subject><issn>0099-2240</issn><issn>1098-5336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEYhFcIREPhxhksJDix4bW9_rogRVEhlYqQ-iFuWK7XTtzurhd706r_HkMiWrhw8uF9NDOeqaqXGOYYE_lhcfRlDkRIWQN_VM0wKFkzSvnjagagVE1IAwfVs5yvAKABLp9WB1g0khLBZ9X382TsdRjWaBXzhM7iNlmXUfRome7GKeYxptCGbY_yOM5RGNCpuUXfzOQS8jGh435M8ca1aOVMN23QacjXaJGzy7l3w_S8euJNl92L_XtYXXw6Ol-u6pOvn4-Xi5PaMoKn2lrMpG8FwQwzwg1pqWyVoK31_LI10AgnqaNeWXACK26N9YJ5EEwpwiWmh9XHne64vexda4t1Mp0eU-hNutPRBP33ZQgbvY43GqvSA8gi8G4vkOKPrcuT7kO2ruvM4OI2awEcFyfxX7CkE0oQVcA3_4BXpdyhtKAJMMUVw6RA73eQTTHn5PyfyBj0r3l1mVf_nlcDL_irh9-8h_d7FuDtHjDZms4nM9iQ7zkpi3PzINwmrDe3ITltcq-N67WgGhNNVcMK9HoHeRO1WacidHFGAFMAIalkDf0JRd3Bkw</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Ruecker, Norma J</creator><creator>Braithwaite, Shannon L</creator><creator>Topp, Edward</creator><creator>Edge, Thomas</creator><creator>Lapen, David R</creator><creator>Wilkes, Graham</creator><creator>Robertson, Will</creator><creator>Medeiros, Diane</creator><creator>Sensen, Christoph W</creator><creator>Neumann, Norman F</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7TV</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20070601</creationdate><title>Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment</title><author>Ruecker, Norma J ; Braithwaite, Shannon L ; Topp, Edward ; Edge, Thomas ; Lapen, David R ; Wilkes, Graham ; Robertson, Will ; Medeiros, Diane ; Sensen, Christoph W ; Neumann, Norman F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-cc158fd72151526a2d38d973dcf6bda047e83e3f9c0e7196cacf75f0759926813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Baileyi</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cluster Analysis</topic><topic>Cryptosporidium</topic><topic>Cryptosporidium - genetics</topic><topic>Cryptosporidium andersoni</topic><topic>Feces - parasitology</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Genotype & phenotype</topic><topic>Health risk assessment</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Ontario</topic><topic>Parasites</topic><topic>Phylogeny</topic><topic>Public Health Microbiology</topic><topic>Ribotyping</topic><topic>Rivers - parasitology</topic><topic>RNA, Ribosomal - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Species Specificity</topic><topic>Watersheds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ruecker, Norma J</creatorcontrib><creatorcontrib>Braithwaite, Shannon L</creatorcontrib><creatorcontrib>Topp, Edward</creatorcontrib><creatorcontrib>Edge, Thomas</creatorcontrib><creatorcontrib>Lapen, David R</creatorcontrib><creatorcontrib>Wilkes, Graham</creatorcontrib><creatorcontrib>Robertson, Will</creatorcontrib><creatorcontrib>Medeiros, Diane</creatorcontrib><creatorcontrib>Sensen, Christoph W</creatorcontrib><creatorcontrib>Neumann, Norman F</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Pollution Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ruecker, Norma J</au><au>Braithwaite, Shannon L</au><au>Topp, Edward</au><au>Edge, Thomas</au><au>Lapen, David R</au><au>Wilkes, Graham</au><au>Robertson, Will</au><au>Medeiros, Diane</au><au>Sensen, Christoph W</au><au>Neumann, Norman F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>73</volume><issue>12</issue><spage>3945</spage><epage>3957</epage><pages>3945-3957</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><coden>AEMIDF</coden><abstract>Recent molecular evidence suggests that different species and/or genotypes of Cryptosporidium display strong host specificity, altering our perceptions regarding the zoonotic potential of this parasite. Molecular forensic profiling of the small-subunit rRNA gene from oocysts enumerated on microscope slides by U.S. Environmental Protection Agency method 1623 was used to identify the range and prevalence of Cryptosporidium species and genotypes in the South Nation watershed in Ontario, Canada. Fourteen sites within the watershed were monitored weekly for 10 weeks to assess the occurrence, molecular composition, and host sources of Cryptosporidium parasites impacting water within the region. Cryptosporidium andersoni, Cryptosporidium muskrat genotype II, Cryptosporidium cervine genotype, C. baileyi, C. parvum, Cryptosporidium muskrat genotype I, the Cryptosporidium fox genotype, genotype W1, and genotype W12 were detected in the watershed. The molecular composition of the Cryptosporidium parasites, supported by general land use analysis, indicated that mature cattle were likely the main source of contamination of the watershed. Deer, muskrats, voles, birds, and other wildlife species, in addition to sewage (human or agricultural) may also potentially impact water quality within the study area. Source water protection studies that use land use analysis with molecular genotyping of Cryptosporidium parasites may provide a more robust source-tracking tool to characterize fecal impacts in a watershed. Moreover, the information is vital for assessing environmental and human health risks posed by water contaminated with zoonotic and/or anthroponotic forms of CRYPTOSPORIDIUM:</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>17483276</pmid><doi>10.1128/AEM.02788-06</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2007-06, Vol.73 (12), p.3945-3957 |
| issn | 0099-2240 1098-5336 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19679729 |
| source | MEDLINE; American Society for Microbiology Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Baileyi Base Sequence Biological and medical sciences Cluster Analysis Cryptosporidium Cryptosporidium - genetics Cryptosporidium andersoni Feces - parasitology Freshwater Fundamental and applied biological sciences. Psychology Genetic Variation Genotype Genotype & phenotype Health risk assessment Microbiology Molecular Sequence Data Ontario Parasites Phylogeny Public Health Microbiology Ribotyping Rivers - parasitology RNA, Ribosomal - genetics Sequence Analysis, DNA Species Specificity Watersheds |
| title | Tracking Host Sources of Cryptosporidium spp. in Raw Water for Improved Health Risk Assessment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-19T00%3A37%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_highw&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Tracking%20Host%20Sources%20of%20Cryptosporidium%20spp.%20in%20Raw%20Water%20for%20Improved%20Health%20Risk%20Assessment&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Ruecker,%20Norma%20J&rft.date=2007-06-01&rft.volume=73&rft.issue=12&rft.spage=3945&rft.epage=3957&rft.pages=3945-3957&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.02788-06&rft_dat=%3Cproquest_highw%3E70612687%3C/proquest_highw%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=205969512&rft_id=info:pmid/17483276&rfr_iscdi=true |