DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi
This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-inf...
Gespeichert in:
| Veröffentlicht in: | Experimental parasitology 2007-07, Vol.116 (3), p.296-301 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 301 |
|---|---|
| container_issue | 3 |
| container_start_page | 296 |
| container_title | Experimental parasitology |
| container_volume | 116 |
| creator | Ribeiro, Daniel A. Calvi, Sueli A. Picka, Mariele M. Persi, Eliana de Carvalho, Thaís B. Caetano, Priscila K. Nagoshi, Lidiana R. Lima, Carlos R.G. Machado, Jussara M. Salvadori, Daisy M.F. |
| description | This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-infected animals);
Trypanosoma cruzi infected;
T. cruzi infected and submitted to benznidazole therapy; and only treated with benznidazole. The results obtained with the single cell gel (comet) assay showed that
T. cruzi was able induce DNA damage in heart cells of both benznidazole treated or untreated infected mice. Similarly,
T. cruzi infected animals showed an increase of DNA lesions in spleen cells. Regarding nitric oxide synthesis, statistically significant differences (
p
<
0.05) were observed in all experimental groups compared to negative control, the strongest effect observed in the
T. cruzi infected group. Taken together, these results indicate that
T. cruzi may increase the level of DNA damage in mice heart and spleen cells. Probably, nitric oxide plays an important role in DNA damaging whereas benznidazole was able to minimize induced
T. cruzi genotoxic effects in spleen cells. |
| doi_str_mv | 10.1016/j.exppara.2006.12.007 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19679148</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014489406003316</els_id><sourcerecordid>19679148</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-4122c7ee516cef0029793065070148edb94f2bba222a4186856bb50c52903c793</originalsourceid><addsrcrecordid>eNqFkE1v1DAQhi0EokvhJ4B8gVvSsddx4hNqS_mQKriUs-VMJtSrxAl2Frr8-nq1kXrkNNLoeefjYeytgFKA0Be7kh7m2UVXSgBdClkC1M_YRoCBQiplnrMNgFCFaow6Y69S2gFAI6R6yc5ELRttarFh-On7Je_c6H4Rd6HjwS_RI58efEc8HcJyT8kn7gPP6yj6kcLihuGQOz3hQh2_ckN7gXz0SPyvX-75XTzMLkxpGh3HuP_nX7MXvRsSvVnrOfv5-ebu-mtx--PLt-vL2wKVVEuhhJRYE1VCI_UA0tRmC7qCOr_RUNca1cu2dVJKp0Sjm0q3bQVYSQNbzOw5-3CaO8fp957SYkefkIbBBZr2yQqja5NHZbA6gRinlCL1ds6fuXiwAuzRrt3Z1a492rVC2mw3596tC_btSN1TatWZgfcr4BK6oY8uoE9PXNNIvdXHSz-eOMo6_niKNqGngNT5mK3abvL_OeUR2UuanQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19679148</pqid></control><display><type>article</type><title>DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Ribeiro, Daniel A. ; Calvi, Sueli A. ; Picka, Mariele M. ; Persi, Eliana ; de Carvalho, Thaís B. ; Caetano, Priscila K. ; Nagoshi, Lidiana R. ; Lima, Carlos R.G. ; Machado, Jussara M. ; Salvadori, Daisy M.F.</creator><creatorcontrib>Ribeiro, Daniel A. ; Calvi, Sueli A. ; Picka, Mariele M. ; Persi, Eliana ; de Carvalho, Thaís B. ; Caetano, Priscila K. ; Nagoshi, Lidiana R. ; Lima, Carlos R.G. ; Machado, Jussara M. ; Salvadori, Daisy M.F.</creatorcontrib><description>This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-infected animals);
Trypanosoma cruzi infected;
T. cruzi infected and submitted to benznidazole therapy; and only treated with benznidazole. The results obtained with the single cell gel (comet) assay showed that
T. cruzi was able induce DNA damage in heart cells of both benznidazole treated or untreated infected mice. Similarly,
T. cruzi infected animals showed an increase of DNA lesions in spleen cells. Regarding nitric oxide synthesis, statistically significant differences (
p
<
0.05) were observed in all experimental groups compared to negative control, the strongest effect observed in the
T. cruzi infected group. Taken together, these results indicate that
T. cruzi may increase the level of DNA damage in mice heart and spleen cells. Probably, nitric oxide plays an important role in DNA damaging whereas benznidazole was able to minimize induced
T. cruzi genotoxic effects in spleen cells.</description><identifier>ISSN: 0014-4894</identifier><identifier>EISSN: 1090-2449</identifier><identifier>DOI: 10.1016/j.exppara.2006.12.007</identifier><identifier>PMID: 17286971</identifier><identifier>CODEN: EXPAAA</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Blood Cells - pathology ; Chagas disease ; Chagas Disease - complications ; Chagas Disease - drug therapy ; Chagas Disease - genetics ; Chagas Disease - metabolism ; DNA Damage ; Fundamental and applied biological sciences. Psychology ; Life cycle. Host-agent relationship. Pathogenesis ; Liver - cytology ; Male ; Mice ; Mice, Inbred BALB C ; Myocardium - cytology ; Neoplasms - etiology ; Nitric oxide ; Nitric Oxide - biosynthesis ; Nitroimidazoles - therapeutic use ; Protozoa ; Random Allocation ; Single cell gel (comet) assay ; Spleen - cytology ; Spleen - metabolism ; Trypanocidal Agents - therapeutic use ; Trypanosoma cruzi ; Trypanosoma cruzi - pathogenicity</subject><ispartof>Experimental parasitology, 2007-07, Vol.116 (3), p.296-301</ispartof><rights>2006 Elsevier Inc.</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-4122c7ee516cef0029793065070148edb94f2bba222a4186856bb50c52903c793</citedby><cites>FETCH-LOGICAL-c424t-4122c7ee516cef0029793065070148edb94f2bba222a4186856bb50c52903c793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.exppara.2006.12.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27922,27923,45993</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18826369$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17286971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ribeiro, Daniel A.</creatorcontrib><creatorcontrib>Calvi, Sueli A.</creatorcontrib><creatorcontrib>Picka, Mariele M.</creatorcontrib><creatorcontrib>Persi, Eliana</creatorcontrib><creatorcontrib>de Carvalho, Thaís B.</creatorcontrib><creatorcontrib>Caetano, Priscila K.</creatorcontrib><creatorcontrib>Nagoshi, Lidiana R.</creatorcontrib><creatorcontrib>Lima, Carlos R.G.</creatorcontrib><creatorcontrib>Machado, Jussara M.</creatorcontrib><creatorcontrib>Salvadori, Daisy M.F.</creatorcontrib><title>DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi</title><title>Experimental parasitology</title><addtitle>Exp Parasitol</addtitle><description>This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-infected animals);
Trypanosoma cruzi infected;
T. cruzi infected and submitted to benznidazole therapy; and only treated with benznidazole. The results obtained with the single cell gel (comet) assay showed that
T. cruzi was able induce DNA damage in heart cells of both benznidazole treated or untreated infected mice. Similarly,
T. cruzi infected animals showed an increase of DNA lesions in spleen cells. Regarding nitric oxide synthesis, statistically significant differences (
p
<
0.05) were observed in all experimental groups compared to negative control, the strongest effect observed in the
T. cruzi infected group. Taken together, these results indicate that
T. cruzi may increase the level of DNA damage in mice heart and spleen cells. Probably, nitric oxide plays an important role in DNA damaging whereas benznidazole was able to minimize induced
T. cruzi genotoxic effects in spleen cells.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Cells - pathology</subject><subject>Chagas disease</subject><subject>Chagas Disease - complications</subject><subject>Chagas Disease - drug therapy</subject><subject>Chagas Disease - genetics</subject><subject>Chagas Disease - metabolism</subject><subject>DNA Damage</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Liver - cytology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Myocardium - cytology</subject><subject>Neoplasms - etiology</subject><subject>Nitric oxide</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitroimidazoles - therapeutic use</subject><subject>Protozoa</subject><subject>Random Allocation</subject><subject>Single cell gel (comet) assay</subject><subject>Spleen - cytology</subject><subject>Spleen - metabolism</subject><subject>Trypanocidal Agents - therapeutic use</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - pathogenicity</subject><issn>0014-4894</issn><issn>1090-2449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhJ4B8gVvSsddx4hNqS_mQKriUs-VMJtSrxAl2Frr8-nq1kXrkNNLoeefjYeytgFKA0Be7kh7m2UVXSgBdClkC1M_YRoCBQiplnrMNgFCFaow6Y69S2gFAI6R6yc5ELRttarFh-On7Je_c6H4Rd6HjwS_RI58efEc8HcJyT8kn7gPP6yj6kcLihuGQOz3hQh2_ckN7gXz0SPyvX-75XTzMLkxpGh3HuP_nX7MXvRsSvVnrOfv5-ebu-mtx--PLt-vL2wKVVEuhhJRYE1VCI_UA0tRmC7qCOr_RUNca1cu2dVJKp0Sjm0q3bQVYSQNbzOw5-3CaO8fp957SYkefkIbBBZr2yQqja5NHZbA6gRinlCL1ds6fuXiwAuzRrt3Z1a492rVC2mw3596tC_btSN1TatWZgfcr4BK6oY8uoE9PXNNIvdXHSz-eOMo6_niKNqGngNT5mK3abvL_OeUR2UuanQ</recordid><startdate>20070701</startdate><enddate>20070701</enddate><creator>Ribeiro, Daniel A.</creator><creator>Calvi, Sueli A.</creator><creator>Picka, Mariele M.</creator><creator>Persi, Eliana</creator><creator>de Carvalho, Thaís B.</creator><creator>Caetano, Priscila K.</creator><creator>Nagoshi, Lidiana R.</creator><creator>Lima, Carlos R.G.</creator><creator>Machado, Jussara M.</creator><creator>Salvadori, Daisy M.F.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope></search><sort><creationdate>20070701</creationdate><title>DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi</title><author>Ribeiro, Daniel A. ; Calvi, Sueli A. ; Picka, Mariele M. ; Persi, Eliana ; de Carvalho, Thaís B. ; Caetano, Priscila K. ; Nagoshi, Lidiana R. ; Lima, Carlos R.G. ; Machado, Jussara M. ; Salvadori, Daisy M.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-4122c7ee516cef0029793065070148edb94f2bba222a4186856bb50c52903c793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Cells - pathology</topic><topic>Chagas disease</topic><topic>Chagas Disease - complications</topic><topic>Chagas Disease - drug therapy</topic><topic>Chagas Disease - genetics</topic><topic>Chagas Disease - metabolism</topic><topic>DNA Damage</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Liver - cytology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Myocardium - cytology</topic><topic>Neoplasms - etiology</topic><topic>Nitric oxide</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitroimidazoles - therapeutic use</topic><topic>Protozoa</topic><topic>Random Allocation</topic><topic>Single cell gel (comet) assay</topic><topic>Spleen - cytology</topic><topic>Spleen - metabolism</topic><topic>Trypanocidal Agents - therapeutic use</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - pathogenicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ribeiro, Daniel A.</creatorcontrib><creatorcontrib>Calvi, Sueli A.</creatorcontrib><creatorcontrib>Picka, Mariele M.</creatorcontrib><creatorcontrib>Persi, Eliana</creatorcontrib><creatorcontrib>de Carvalho, Thaís B.</creatorcontrib><creatorcontrib>Caetano, Priscila K.</creatorcontrib><creatorcontrib>Nagoshi, Lidiana R.</creatorcontrib><creatorcontrib>Lima, Carlos R.G.</creatorcontrib><creatorcontrib>Machado, Jussara M.</creatorcontrib><creatorcontrib>Salvadori, Daisy M.F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Experimental parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ribeiro, Daniel A.</au><au>Calvi, Sueli A.</au><au>Picka, Mariele M.</au><au>Persi, Eliana</au><au>de Carvalho, Thaís B.</au><au>Caetano, Priscila K.</au><au>Nagoshi, Lidiana R.</au><au>Lima, Carlos R.G.</au><au>Machado, Jussara M.</au><au>Salvadori, Daisy M.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi</atitle><jtitle>Experimental parasitology</jtitle><addtitle>Exp Parasitol</addtitle><date>2007-07-01</date><risdate>2007</risdate><volume>116</volume><issue>3</issue><spage>296</spage><epage>301</epage><pages>296-301</pages><issn>0014-4894</issn><eissn>1090-2449</eissn><coden>EXPAAA</coden><abstract>This study aimed to evaluate whether experimental Chagas disease in acute phase under benznidazole therapy can cause DNA damage in peripheral blood, liver, heart, and spleen cells or induce nitric oxide synthesis in spleen cells. Twenty Balb/c mice were distributed into four groups: control (non-infected animals);
Trypanosoma cruzi infected;
T. cruzi infected and submitted to benznidazole therapy; and only treated with benznidazole. The results obtained with the single cell gel (comet) assay showed that
T. cruzi was able induce DNA damage in heart cells of both benznidazole treated or untreated infected mice. Similarly,
T. cruzi infected animals showed an increase of DNA lesions in spleen cells. Regarding nitric oxide synthesis, statistically significant differences (
p
<
0.05) were observed in all experimental groups compared to negative control, the strongest effect observed in the
T. cruzi infected group. Taken together, these results indicate that
T. cruzi may increase the level of DNA damage in mice heart and spleen cells. Probably, nitric oxide plays an important role in DNA damaging whereas benznidazole was able to minimize induced
T. cruzi genotoxic effects in spleen cells.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>17286971</pmid><doi>10.1016/j.exppara.2006.12.007</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-4894 |
| ispartof | Experimental parasitology, 2007-07, Vol.116 (3), p.296-301 |
| issn | 0014-4894 1090-2449 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19679148 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | Animals Biological and medical sciences Blood Cells - pathology Chagas disease Chagas Disease - complications Chagas Disease - drug therapy Chagas Disease - genetics Chagas Disease - metabolism DNA Damage Fundamental and applied biological sciences. Psychology Life cycle. Host-agent relationship. Pathogenesis Liver - cytology Male Mice Mice, Inbred BALB C Myocardium - cytology Neoplasms - etiology Nitric oxide Nitric Oxide - biosynthesis Nitroimidazoles - therapeutic use Protozoa Random Allocation Single cell gel (comet) assay Spleen - cytology Spleen - metabolism Trypanocidal Agents - therapeutic use Trypanosoma cruzi Trypanosoma cruzi - pathogenicity |
| title | DNA damage and nitric oxide synthesis in experimentally infected Balb/c mice with Trypanosoma cruzi |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T12%3A27%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20damage%20and%20nitric%20oxide%20synthesis%20in%20experimentally%20infected%20Balb/c%20mice%20with%20Trypanosoma%20cruzi&rft.jtitle=Experimental%20parasitology&rft.au=Ribeiro,%20Daniel%20A.&rft.date=2007-07-01&rft.volume=116&rft.issue=3&rft.spage=296&rft.epage=301&rft.pages=296-301&rft.issn=0014-4894&rft.eissn=1090-2449&rft.coden=EXPAAA&rft_id=info:doi/10.1016/j.exppara.2006.12.007&rft_dat=%3Cproquest_cross%3E19679148%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19679148&rft_id=info:pmid/17286971&rft_els_id=S0014489406003316&rfr_iscdi=true |