A microbeam study of DNA double-strand breaks in bystander primary human fibroblasts
Radiation-induced bystander effect has been well documented. However, the mechanisms are poorly understood. How we incorporate this effect into the classical models of risk assessment remains an open question. Here, the induction of bystander effect was studied by assessing DNA double-strand break (...
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| Veröffentlicht in: | Radiation protection dosimetry 2006-12, Vol.122 (1-4), p.256-259 |
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| container_title | Radiation protection dosimetry |
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| creator | Smilenov, L. B. Hall, E. J. Bonner, W. M. Sedelnikova, O. A. |
| description | Radiation-induced bystander effect has been well documented. However, the mechanisms are poorly understood. How we incorporate this effect into the classical models of risk assessment remains an open question. Here, the induction of bystander effect was studied by assessing DNA double-strand break (DSB) formation in situ with the rapid and sensitive γ-H2AX focus formation assay. Utilising the Columbia University single-cell microbeam system to deliver 2 or 20 individual alpha particles to selected cell nuclei in a precisely known proportion of cells in a population, the induced DNA DSB incidences were quantified 30 min and 18 h post-IR. The increase in DNA DSB incidence in bystander cells lacked of a linear dose response indicating that neither the dose of irradiation nor proportion of irradiated cells in a population, is a critical parameter. This study confirms a binary all-or-nothing model of triggering the bystander response. The delay and persistence of the bystander response suggests a different mechanism of DSB induction in bystander cells than in directly irradiated cells. |
| doi_str_mv | 10.1093/rpd/ncl461 |
| format | Article |
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The increase in DNA DSB incidence in bystander cells lacked of a linear dose response indicating that neither the dose of irradiation nor proportion of irradiated cells in a population, is a critical parameter. This study confirms a binary all-or-nothing model of triggering the bystander response. The delay and persistence of the bystander response suggests a different mechanism of DSB induction in bystander cells than in directly irradiated cells.</description><identifier>ISSN: 0144-8420</identifier><identifier>EISSN: 1742-3406</identifier><identifier>DOI: 10.1093/rpd/ncl461</identifier><identifier>PMID: 17164279</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Alpha Particles ; Bystander Effect - physiology ; Bystander Effect - radiation effects ; Cell Line ; DNA - genetics ; DNA - radiation effects ; DNA Damage ; Dose-Response Relationship, Radiation ; Fibroblasts - physiology ; Fibroblasts - radiation effects ; Humans ; Radiation Dosage ; Radiation Tolerance - physiology ; Radiation Tolerance - radiation effects</subject><ispartof>Radiation protection dosimetry, 2006-12, Vol.122 (1-4), p.256-259</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-a2135a31d34e178aba65fe8bff14a587d68cc90031e8c71fd8eab250632f36383</citedby><cites>FETCH-LOGICAL-c420t-a2135a31d34e178aba65fe8bff14a587d68cc90031e8c71fd8eab250632f36383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17164279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Smilenov, L. B.</creatorcontrib><creatorcontrib>Hall, E. J.</creatorcontrib><creatorcontrib>Bonner, W. M.</creatorcontrib><creatorcontrib>Sedelnikova, O. A.</creatorcontrib><title>A microbeam study of DNA double-strand breaks in bystander primary human fibroblasts</title><title>Radiation protection dosimetry</title><addtitle>Radiat Prot Dosimetry</addtitle><description>Radiation-induced bystander effect has been well documented. However, the mechanisms are poorly understood. How we incorporate this effect into the classical models of risk assessment remains an open question. Here, the induction of bystander effect was studied by assessing DNA double-strand break (DSB) formation in situ with the rapid and sensitive γ-H2AX focus formation assay. Utilising the Columbia University single-cell microbeam system to deliver 2 or 20 individual alpha particles to selected cell nuclei in a precisely known proportion of cells in a population, the induced DNA DSB incidences were quantified 30 min and 18 h post-IR. The increase in DNA DSB incidence in bystander cells lacked of a linear dose response indicating that neither the dose of irradiation nor proportion of irradiated cells in a population, is a critical parameter. This study confirms a binary all-or-nothing model of triggering the bystander response. The delay and persistence of the bystander response suggests a different mechanism of DSB induction in bystander cells than in directly irradiated cells.</description><subject>Alpha Particles</subject><subject>Bystander Effect - physiology</subject><subject>Bystander Effect - radiation effects</subject><subject>Cell Line</subject><subject>DNA - genetics</subject><subject>DNA - radiation effects</subject><subject>DNA Damage</subject><subject>Dose-Response Relationship, Radiation</subject><subject>Fibroblasts - physiology</subject><subject>Fibroblasts - radiation effects</subject><subject>Humans</subject><subject>Radiation Dosage</subject><subject>Radiation Tolerance - physiology</subject><subject>Radiation Tolerance - radiation effects</subject><issn>0144-8420</issn><issn>1742-3406</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkE1LAzEQhoMoWqsXf4Dk5EFYm9lkk-yx1I-KRT1UKl5Cspvg6n7UZBfsvzfSoqdhZh5eZh6EzoBcAcnpxK_LSVvUjMMeGoFgaUIZ4ftoRICxRLKUHKHjED4ISUWesUN0BAI4i80ILae4qQrfGasbHPqh3ODO4evHKS67wdQ2Cb3XbYmNt_oz4KrFZhP6OLEer33VaL_B70OjW-wqE2NqHfpwgg6croM93dUxerm9Wc7myeLp7n42XSRFvKlPdAo00xRKyiwIqY3mmbPSOAdMZ1KUXBZFTggFKwsBrpRWmzQjnKaOcirpGF1sc9e--xps6FVThcLWtW5tNwQFORc5kRDByy0YPw3BW6d2tysg6tehig7V1mGEz3epg2ls-Y_upEUg2QJV6O333177T8UFFZmav74pYCvOVw_Pakl_AMy-fSg</recordid><startdate>20061201</startdate><enddate>20061201</enddate><creator>Smilenov, L. B.</creator><creator>Hall, E. J.</creator><creator>Bonner, W. M.</creator><creator>Sedelnikova, O. A.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>20061201</creationdate><title>A microbeam study of DNA double-strand breaks in bystander primary human fibroblasts</title><author>Smilenov, L. B. ; Hall, E. J. ; Bonner, W. M. ; Sedelnikova, O. 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J.</creatorcontrib><creatorcontrib>Bonner, W. M.</creatorcontrib><creatorcontrib>Sedelnikova, O. A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Radiation protection dosimetry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smilenov, L. B.</au><au>Hall, E. J.</au><au>Bonner, W. M.</au><au>Sedelnikova, O. 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| ispartof | Radiation protection dosimetry, 2006-12, Vol.122 (1-4), p.256-259 |
| issn | 0144-8420 1742-3406 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19679081 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Alpha Particles Bystander Effect - physiology Bystander Effect - radiation effects Cell Line DNA - genetics DNA - radiation effects DNA Damage Dose-Response Relationship, Radiation Fibroblasts - physiology Fibroblasts - radiation effects Humans Radiation Dosage Radiation Tolerance - physiology Radiation Tolerance - radiation effects |
| title | A microbeam study of DNA double-strand breaks in bystander primary human fibroblasts |
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