Candida khmerensis sp. nov., a novel cation-tolerant yeast isolated from dry salted shrimp and sewage in Cambodia
Two cation-tolerant yeasts with powdered colonies, K28-3-2T and K26-1-4, were isolated from dry salted shrimp and sewage, respectively, in Siem Reap province, Cambodia. The D1/D2 sequences of the 26S rDNA data showed that the two isolates were conspecific and related to the Pichia burtonii and Candi...
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| Veröffentlicht in: | Journal of general and applied microbiology 2005, Vol.51(4), pp.235-243 |
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| creator | Nagatsuka, Yuka Kawasaki, Hiroko Mikata, Kozaburo Seki, Tatsuji |
| description | Two cation-tolerant yeasts with powdered colonies, K28-3-2T and K26-1-4, were isolated from dry salted shrimp and sewage, respectively, in Siem Reap province, Cambodia. The D1/D2 sequences of the 26S rDNA data showed that the two isolates were conspecific and related to the Pichia burtonii and Candida fennica. Two isolates were examined by a polyphasic taxonomic approach, including molecular phylogenetic analysis, morphological, physiological and biochemical tests, DNA hybridization and MSP-PCR fingerprinting, in comparison with P. burtonii and C. fennica. The two isolates were found to grow by multilateral budding with true and pseudo-mycelium, to not produce ascospores, and to contain ubiquinone Q-8 similar to that of P. burtonii and C. fennica. The two isolates were not differentiated from the two closest species, P. burtonii and C. fennica, by the phenotypic character examined, except for the cation (Li+)-tolerance. From DNA-DNA reassociation studies, however, the two isolates showed low similarities to the closest two species. Based on D1/D2 sequences of 26S rDNA and DNA-DNA reassociation data, they were shown to be a new distinct species from P. burtonii and C. fennica. Therefore, a novel species is proposed, Candida khmerensis sp. nov., represented by strain K28-3-2T (=JCM 13262T=CBS 9784T). The novel species, Candida khmerensis sp. nov. can be clearly distinguished from P. burtonii and C. fennica by either the 26S rDNA D1/D2 or ITS region with 5.8S rDNA sequencing, or by the MSP-PCR fingerprinting pattern. |
| doi_str_mv | 10.2323/jgam.51.235 |
| format | Article |
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The D1/D2 sequences of the 26S rDNA data showed that the two isolates were conspecific and related to the Pichia burtonii and Candida fennica. Two isolates were examined by a polyphasic taxonomic approach, including molecular phylogenetic analysis, morphological, physiological and biochemical tests, DNA hybridization and MSP-PCR fingerprinting, in comparison with P. burtonii and C. fennica. The two isolates were found to grow by multilateral budding with true and pseudo-mycelium, to not produce ascospores, and to contain ubiquinone Q-8 similar to that of P. burtonii and C. fennica. The two isolates were not differentiated from the two closest species, P. burtonii and C. fennica, by the phenotypic character examined, except for the cation (Li+)-tolerance. From DNA-DNA reassociation studies, however, the two isolates showed low similarities to the closest two species. Based on D1/D2 sequences of 26S rDNA and DNA-DNA reassociation data, they were shown to be a new distinct species from P. burtonii and C. fennica. Therefore, a novel species is proposed, Candida khmerensis sp. nov., represented by strain K28-3-2T (=JCM 13262T=CBS 9784T). The novel species, Candida khmerensis sp. nov. can be clearly distinguished from P. burtonii and C. fennica by either the 26S rDNA D1/D2 or ITS region with 5.8S rDNA sequencing, or by the MSP-PCR fingerprinting pattern.</description><identifier>ISSN: 0022-1260</identifier><identifier>EISSN: 1349-8037</identifier><identifier>DOI: 10.2323/jgam.51.235</identifier><identifier>PMID: 16205031</identifier><identifier>CODEN: JGAMA9</identifier><language>eng</language><publisher>Tokyo: Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</publisher><subject>Base Sequence ; Biological and medical sciences ; Cambodia ; Candida ; Candida - classification ; Candida - isolation & purification ; Candida - physiology ; Candida fennica ; Candida khmerensis ; Candida khmerensis sp. nov ; cation-tolerance ; Cations - pharmacology ; DNA, Fungal - genetics ; DNA, Ribosomal Spacer - genetics ; Food Microbiology ; Fundamental and applied biological sciences. Psychology ; halo-tolerance ; Lithium - pharmacology ; Microbiology ; Molecular Sequence Data ; MSP-PCR ; osmo-tolerance ; Penaeidae - microbiology ; Phylogeny ; Pichia ; Pichia burtonii ; RNA, Fungal - genetics ; RNA, Ribosomal - genetics ; Sequence Alignment ; Sewage - microbiology ; Shellfish - microbiology ; Sodium Chloride, Dietary ; Species Specificity</subject><ispartof>The Journal of General and Applied Microbiology, 2005, Vol.51(4), pp.235-243</ispartof><rights>2005 by The Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c593t-b98a482cf07e720f40a151152c499d60955ff478b96b4c38edf33d75f8eb9c073</citedby><cites>FETCH-LOGICAL-c593t-b98a482cf07e720f40a151152c499d60955ff478b96b4c38edf33d75f8eb9c073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17225570$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16205031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagatsuka, Yuka</creatorcontrib><creatorcontrib>Kawasaki, Hiroko</creatorcontrib><creatorcontrib>Mikata, Kozaburo</creatorcontrib><creatorcontrib>Seki, Tatsuji</creatorcontrib><title>Candida khmerensis sp. nov., a novel cation-tolerant yeast isolated from dry salted shrimp and sewage in Cambodia</title><title>Journal of general and applied microbiology</title><addtitle>J. Gen. Appl. Microbiol.</addtitle><description>Two cation-tolerant yeasts with powdered colonies, K28-3-2T and K26-1-4, were isolated from dry salted shrimp and sewage, respectively, in Siem Reap province, Cambodia. The D1/D2 sequences of the 26S rDNA data showed that the two isolates were conspecific and related to the Pichia burtonii and Candida fennica. Two isolates were examined by a polyphasic taxonomic approach, including molecular phylogenetic analysis, morphological, physiological and biochemical tests, DNA hybridization and MSP-PCR fingerprinting, in comparison with P. burtonii and C. fennica. The two isolates were found to grow by multilateral budding with true and pseudo-mycelium, to not produce ascospores, and to contain ubiquinone Q-8 similar to that of P. burtonii and C. fennica. The two isolates were not differentiated from the two closest species, P. burtonii and C. fennica, by the phenotypic character examined, except for the cation (Li+)-tolerance. From DNA-DNA reassociation studies, however, the two isolates showed low similarities to the closest two species. Based on D1/D2 sequences of 26S rDNA and DNA-DNA reassociation data, they were shown to be a new distinct species from P. burtonii and C. fennica. Therefore, a novel species is proposed, Candida khmerensis sp. nov., represented by strain K28-3-2T (=JCM 13262T=CBS 9784T). The novel species, Candida khmerensis sp. nov. can be clearly distinguished from P. burtonii and C. fennica by either the 26S rDNA D1/D2 or ITS region with 5.8S rDNA sequencing, or by the MSP-PCR fingerprinting pattern.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cambodia</subject><subject>Candida</subject><subject>Candida - classification</subject><subject>Candida - isolation & purification</subject><subject>Candida - physiology</subject><subject>Candida fennica</subject><subject>Candida khmerensis</subject><subject>Candida khmerensis sp. nov</subject><subject>cation-tolerance</subject><subject>Cations - pharmacology</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Food Microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>halo-tolerance</subject><subject>Lithium - pharmacology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>MSP-PCR</subject><subject>osmo-tolerance</subject><subject>Penaeidae - microbiology</subject><subject>Phylogeny</subject><subject>Pichia</subject><subject>Pichia burtonii</subject><subject>RNA, Fungal - genetics</subject><subject>RNA, Ribosomal - genetics</subject><subject>Sequence Alignment</subject><subject>Sewage - microbiology</subject><subject>Shellfish - microbiology</subject><subject>Sodium Chloride, Dietary</subject><subject>Species Specificity</subject><issn>0022-1260</issn><issn>1349-8037</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc2P0zAQxS0EYsvCiTuyhOACKf6I4_gGqviSVuIC52jijLcuSdz1pKD-97hqtStxmdHT_PTGfsPYSynWSiv9YXcL09rIIswjtpK6dlUrtH3MVkIoVUnViCv2jGgnhG5UWz9lV7JRwggtV-xuA_MQB-C_txNmnCkSp_2az-nP-j2HU8eRe1himqsljZhhXvgRgRYeKY2w4MBDThMf8pETjCdN2xynPS_OnPAv3CKPM9_A1KchwnP2JMBI-OLSr9mvL59_br5VNz--ft98uqm8cXqpetdC3SofhEWrRKgFSCOlUb52bmiEMyaE2ra9a_ra6xaHoPVgTWixd15Yfc3enn33Od0dkJZuiuRxHGHGdKBOusa21qkCvv4P3KVDnsvbOllrVxITsi3UuzPlcyLKGLp9-STkYydFd7pDd7pDZ2QRptCvLp6HfsLhgb0EX4A3FwDIwxhKrD7SA2eVMsaKwn08cztaSpD3AOQl-hHvl9bnUnbfj_wWcoez_gfH2acu</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>Nagatsuka, Yuka</creator><creator>Kawasaki, Hiroko</creator><creator>Mikata, Kozaburo</creator><creator>Seki, Tatsuji</creator><general>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</general><general>Microbiology Research Foundation</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>F1W</scope><scope>H95</scope><scope>L.G</scope></search><sort><creationdate>20050101</creationdate><title>Candida khmerensis sp. nov., a novel cation-tolerant yeast isolated from dry salted shrimp and sewage in Cambodia</title><author>Nagatsuka, Yuka ; Kawasaki, Hiroko ; Mikata, Kozaburo ; Seki, Tatsuji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c593t-b98a482cf07e720f40a151152c499d60955ff478b96b4c38edf33d75f8eb9c073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cambodia</topic><topic>Candida</topic><topic>Candida - classification</topic><topic>Candida - isolation & purification</topic><topic>Candida - physiology</topic><topic>Candida fennica</topic><topic>Candida khmerensis</topic><topic>Candida khmerensis sp. nov</topic><topic>cation-tolerance</topic><topic>Cations - pharmacology</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Food Microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>halo-tolerance</topic><topic>Lithium - pharmacology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>MSP-PCR</topic><topic>osmo-tolerance</topic><topic>Penaeidae - microbiology</topic><topic>Phylogeny</topic><topic>Pichia</topic><topic>Pichia burtonii</topic><topic>RNA, Fungal - genetics</topic><topic>RNA, Ribosomal - genetics</topic><topic>Sequence Alignment</topic><topic>Sewage - microbiology</topic><topic>Shellfish - microbiology</topic><topic>Sodium Chloride, Dietary</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nagatsuka, Yuka</creatorcontrib><creatorcontrib>Kawasaki, Hiroko</creatorcontrib><creatorcontrib>Mikata, Kozaburo</creatorcontrib><creatorcontrib>Seki, Tatsuji</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><jtitle>Journal of general and applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nagatsuka, Yuka</au><au>Kawasaki, Hiroko</au><au>Mikata, Kozaburo</au><au>Seki, Tatsuji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Candida khmerensis sp. nov., a novel cation-tolerant yeast isolated from dry salted shrimp and sewage in Cambodia</atitle><jtitle>Journal of general and applied microbiology</jtitle><addtitle>J. Gen. Appl. Microbiol.</addtitle><date>2005-01-01</date><risdate>2005</risdate><volume>51</volume><issue>4</issue><spage>235</spage><epage>243</epage><pages>235-243</pages><issn>0022-1260</issn><eissn>1349-8037</eissn><coden>JGAMA9</coden><abstract>Two cation-tolerant yeasts with powdered colonies, K28-3-2T and K26-1-4, were isolated from dry salted shrimp and sewage, respectively, in Siem Reap province, Cambodia. The D1/D2 sequences of the 26S rDNA data showed that the two isolates were conspecific and related to the Pichia burtonii and Candida fennica. Two isolates were examined by a polyphasic taxonomic approach, including molecular phylogenetic analysis, morphological, physiological and biochemical tests, DNA hybridization and MSP-PCR fingerprinting, in comparison with P. burtonii and C. fennica. The two isolates were found to grow by multilateral budding with true and pseudo-mycelium, to not produce ascospores, and to contain ubiquinone Q-8 similar to that of P. burtonii and C. fennica. The two isolates were not differentiated from the two closest species, P. burtonii and C. fennica, by the phenotypic character examined, except for the cation (Li+)-tolerance. From DNA-DNA reassociation studies, however, the two isolates showed low similarities to the closest two species. Based on D1/D2 sequences of 26S rDNA and DNA-DNA reassociation data, they were shown to be a new distinct species from P. burtonii and C. fennica. Therefore, a novel species is proposed, Candida khmerensis sp. nov., represented by strain K28-3-2T (=JCM 13262T=CBS 9784T). The novel species, Candida khmerensis sp. nov. can be clearly distinguished from P. burtonii and C. fennica by either the 26S rDNA D1/D2 or ITS region with 5.8S rDNA sequencing, or by the MSP-PCR fingerprinting pattern.</abstract><cop>Tokyo</cop><pub>Applied Microbiology, Molecular and Cellular Biosciences Research Foundation</pub><pmid>16205031</pmid><doi>10.2323/jgam.51.235</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese |
| subjects | Base Sequence Biological and medical sciences Cambodia Candida Candida - classification Candida - isolation & purification Candida - physiology Candida fennica Candida khmerensis Candida khmerensis sp. nov cation-tolerance Cations - pharmacology DNA, Fungal - genetics DNA, Ribosomal Spacer - genetics Food Microbiology Fundamental and applied biological sciences. Psychology halo-tolerance Lithium - pharmacology Microbiology Molecular Sequence Data MSP-PCR osmo-tolerance Penaeidae - microbiology Phylogeny Pichia Pichia burtonii RNA, Fungal - genetics RNA, Ribosomal - genetics Sequence Alignment Sewage - microbiology Shellfish - microbiology Sodium Chloride, Dietary Species Specificity |
| title | Candida khmerensis sp. nov., a novel cation-tolerant yeast isolated from dry salted shrimp and sewage in Cambodia |
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