Novel polyubiquitin imaging system, PolyUb-FC, reveals that K33-linked polyubiquitin is recruited by SQSTM1/p62

Ubiquitin chains are formed with 8 structurally and functionally distinct polymers. However, the functions of each polyubiquitin remain poorly understood. We developed a polyubiquitin-mediated fluorescence complementation (PolyUb-FC) assay using Kusabira Green (KG) as a split fluorescent protein. Th...

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Veröffentlicht in:	Autophagy 2018-01, Vol.14 (2), p.347-358
Hauptverfasser:	Nibe, Yoichi, Oshima, Shigeru, Kobayashi, Masanori, Maeyashiki, Chiaki, Matsuzawa, Yu, Otsubo, Kana, Matsuda, Hiroki, Aonuma, Emi, Nemoto, Yasuhiro, Nagaishi, Takashi, Okamoto, Ryuichi, Tsuchiya, Kiichiro, Nakamura, Tetsuya, Nakada, Shinichiro, Watanabe, Mamoru
Format:	Artikel
Sprache:	eng
Schlagworte:	autophagy BiFC SQSTM1/p62 Toolbox ubiquitin
Online-Zugang:	Volltext
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