Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA
We showcase the high potential of the 2'-cyanoethoxymethyl (CEM) methodology to synthesize RNAs with naturally occurring modified residues carrying stable isotope (SI) labels for NMR spectroscopic applications. The method was applied to synthesize RNAs with sizes ranging between 60 to 80 nucleo...
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| Veröffentlicht in: | Chemical communications (Cambridge, England) England), 2017, Vol.53 (96), p.12938-12941 |
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| container_title | Chemical communications (Cambridge, England) |
| container_volume | 53 |
| creator | Kremser, J Strebitzer, E Plangger, R Juen, M A Nußbaumer, F Glasner, H Breuker, K Kreutz, C |
| description | We showcase the high potential of the 2'-cyanoethoxymethyl (CEM) methodology to synthesize RNAs with naturally occurring modified residues carrying stable isotope (SI) labels for NMR spectroscopic applications. The method was applied to synthesize RNAs with sizes ranging between 60 to 80 nucleotides. The presented approach gives the possibility to selectively modify larger RNAs (>60 nucleotides) with atom-specifically
C/
N-labelled building blocks. The method harbors the unique potential to address structural as well as dynamic features of these RNAs with NMR spectroscopy but also using other biophysical methods, such as mass spectrometry (MS), or small angle neutron/X-ray scattering (SANS, SAXS). |
| doi_str_mv | 10.1039/c7cc06747j |
| format | Article |
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C/
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C/
N-labelled building blocks. 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C/
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| ispartof | Chemical communications (Cambridge, England), 2017, Vol.53 (96), p.12938-12941 |
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| language | eng |
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| source | MEDLINE; Royal Society Of Chemistry Journals; Alma/SFX Local Collection |
| subjects | Carbon Isotopes Chemical synthesis Magnetic Resonance Spectroscopy Mass spectrometry Nitrogen Isotopes NMR NMR spectroscopy Nuclear magnetic resonance Nucleotides Ribonucleic acid RNA RNA - chemical synthesis RNA - chemistry Scattering, Small Angle Small angle X ray scattering X-Ray Diffraction |
| title | Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA |
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