Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis
Objective To evaluate the value of serum microRNA‐122 (miR‐122) and miR‐199a as reliable noninvasive biomarkers in the diagnosis of endometriosis. Methods During 2015–2016, at a teaching hospital in Egypt, a prospective cohort study was conducted on 45 women with pelvic endometriosis and 35 women wh...
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| Veröffentlicht in: | International journal of gynecology and obstetrics 2018-04, Vol.141 (1), p.14-19 |
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| container_title | International journal of gynecology and obstetrics |
| container_volume | 141 |
| creator | Maged, Ahmed M. Deeb, Wesam S. El Amir, Azza Zaki, Sherif S. El Sawah, Heba Al Mohamady, Maged Metwally, Ahmed A. Katta, Maha A. |
| description | Objective
To evaluate the value of serum microRNA‐122 (miR‐122) and miR‐199a as reliable noninvasive biomarkers in the diagnosis of endometriosis.
Methods
During 2015–2016, at a teaching hospital in Egypt, a prospective cohort study was conducted on 45 women with pelvic endometriosis and 35 women who underwent laparoscopy for pelvic pain but were not diagnosed with endometriosis. Blood and peritoneal fluid (PF) samples were collected; interleukin‐6 (IL‐6) was detected by enzyme‐linked immunosorbent assay and miR‐122 and miR‐199a expression was measured by quantitative real‐time polymerase chain reaction.
Results
The serum and PF levels of IL‐6, miR‐122, and miR‐199a were significantly higher in women with endometriosis than in controls (P |
| doi_str_mv | 10.1002/ijgo.12392 |
| format | Article |
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To evaluate the value of serum microRNA‐122 (miR‐122) and miR‐199a as reliable noninvasive biomarkers in the diagnosis of endometriosis.
Methods
During 2015–2016, at a teaching hospital in Egypt, a prospective cohort study was conducted on 45 women with pelvic endometriosis and 35 women who underwent laparoscopy for pelvic pain but were not diagnosed with endometriosis. Blood and peritoneal fluid (PF) samples were collected; interleukin‐6 (IL‐6) was detected by enzyme‐linked immunosorbent assay and miR‐122 and miR‐199a expression was measured by quantitative real‐time polymerase chain reaction.
Results
The serum and PF levels of IL‐6, miR‐122, and miR‐199a were significantly higher in women with endometriosis than in controls (P<0.001 for all comparisons). Serum miR‐122 expression was positively correlated with serum IL‐6 (r=0.597), PF IL‐6 (r=0.603), PF miR‐122 (r=0.934), serum miR‐199a (r=0.727), and PF miR‐199a (r=0.653). Serum miR‐199a expression was positively correlated with serum IL‐6 (r=0.677), PF IL‐6 (r=0.678), PF miR‐122 (r=0.744), and PF miR‐199a (r=0.932). Serum miR‐122 and miR‐199a had a sensitivity of 95.6% and 100.0%, and a specificity of 91.4% and 100%, respectively, for the detection of endometriosis.
Conclusion
Serum miR‐122 and miR‐199a were significantly increased in endometriosis, indicating that these microRNAs might serve as biomarkers for the diagnosis of endometriosis.
Serum miR‐122 and miR‐199a are significantly increased in women with endometriosis and these microRNAs have a strong potential to act as biomarkers to diagnose endometriosis.</description><identifier>ISSN: 0020-7292</identifier><identifier>EISSN: 1879-3479</identifier><identifier>DOI: 10.1002/ijgo.12392</identifier><identifier>PMID: 29149541</identifier><language>eng</language><publisher>United States</publisher><subject>Endometriosis ; MicroRNA ; miR‐122 ; miR‐199a ; Pelvic pain</subject><ispartof>International journal of gynecology and obstetrics, 2018-04, Vol.141 (1), p.14-19</ispartof><rights>2017 International Federation of Gynecology and Obstetrics</rights><rights>2017 International Federation of Gynecology and Obstetrics.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3552-1079ba878361843519d1066a1a4261f90d99756f5e02b458a86ff6a09fceeb6c3</citedby><cites>FETCH-LOGICAL-c3552-1079ba878361843519d1066a1a4261f90d99756f5e02b458a86ff6a09fceeb6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijgo.12392$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijgo.12392$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29149541$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maged, Ahmed M.</creatorcontrib><creatorcontrib>Deeb, Wesam S.</creatorcontrib><creatorcontrib>El Amir, Azza</creatorcontrib><creatorcontrib>Zaki, Sherif S.</creatorcontrib><creatorcontrib>El Sawah, Heba</creatorcontrib><creatorcontrib>Al Mohamady, Maged</creatorcontrib><creatorcontrib>Metwally, Ahmed A.</creatorcontrib><creatorcontrib>Katta, Maha A.</creatorcontrib><title>Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis</title><title>International journal of gynecology and obstetrics</title><addtitle>Int J Gynaecol Obstet</addtitle><description>Objective
To evaluate the value of serum microRNA‐122 (miR‐122) and miR‐199a as reliable noninvasive biomarkers in the diagnosis of endometriosis.
Methods
During 2015–2016, at a teaching hospital in Egypt, a prospective cohort study was conducted on 45 women with pelvic endometriosis and 35 women who underwent laparoscopy for pelvic pain but were not diagnosed with endometriosis. Blood and peritoneal fluid (PF) samples were collected; interleukin‐6 (IL‐6) was detected by enzyme‐linked immunosorbent assay and miR‐122 and miR‐199a expression was measured by quantitative real‐time polymerase chain reaction.
Results
The serum and PF levels of IL‐6, miR‐122, and miR‐199a were significantly higher in women with endometriosis than in controls (P<0.001 for all comparisons). Serum miR‐122 expression was positively correlated with serum IL‐6 (r=0.597), PF IL‐6 (r=0.603), PF miR‐122 (r=0.934), serum miR‐199a (r=0.727), and PF miR‐199a (r=0.653). Serum miR‐199a expression was positively correlated with serum IL‐6 (r=0.677), PF IL‐6 (r=0.678), PF miR‐122 (r=0.744), and PF miR‐199a (r=0.932). Serum miR‐122 and miR‐199a had a sensitivity of 95.6% and 100.0%, and a specificity of 91.4% and 100%, respectively, for the detection of endometriosis.
Conclusion
Serum miR‐122 and miR‐199a were significantly increased in endometriosis, indicating that these microRNAs might serve as biomarkers for the diagnosis of endometriosis.
Serum miR‐122 and miR‐199a are significantly increased in women with endometriosis and these microRNAs have a strong potential to act as biomarkers to diagnose endometriosis.</description><subject>Endometriosis</subject><subject>MicroRNA</subject><subject>miR‐122</subject><subject>miR‐199a</subject><subject>Pelvic pain</subject><issn>0020-7292</issn><issn>1879-3479</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kLFOwzAQQC0EoqWw8AHII0JK8TmJHY-oQClCqkAwW47jFFdJXOJEVTc-gW_kS0hJy8hyp9M9veEhdA5kDITQa7tcuDHQUNADNISEiyCMuDhEw-5JAk4FHaAT75eEEOAAx2hABUQijmCInm-tWlTON1ZjpXVbK73BLsfe1G2JS_vy_fkFlGJVZftLCIVthdeuNN20zTs2VdYdTW2dt_4UHeWq8OZst0fo7f7udfIQPM2ns8nNU6DDOKYBEC5SlfAkZJBEYQwiA8KYAhVRBrkgmRA8ZnlsCE2jOFEJy3OmiMi1MSnT4Qhd9t5V7T5a4xtZWq9NUajKuNZLEIzRkHJGOvSqR3XtvK9NLle1LVW9kUDkNqHcJpS_CTv4Yudt09Jkf-i-WQdAD6xtYTb_qOTscTrvpT_zDXuI</recordid><startdate>201804</startdate><enddate>201804</enddate><creator>Maged, Ahmed M.</creator><creator>Deeb, Wesam S.</creator><creator>El Amir, Azza</creator><creator>Zaki, Sherif S.</creator><creator>El Sawah, Heba</creator><creator>Al Mohamady, Maged</creator><creator>Metwally, Ahmed A.</creator><creator>Katta, Maha A.</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201804</creationdate><title>Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis</title><author>Maged, Ahmed M. ; Deeb, Wesam S. ; El Amir, Azza ; Zaki, Sherif S. ; El Sawah, Heba ; Al Mohamady, Maged ; Metwally, Ahmed A. ; Katta, Maha A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3552-1079ba878361843519d1066a1a4261f90d99756f5e02b458a86ff6a09fceeb6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Endometriosis</topic><topic>MicroRNA</topic><topic>miR‐122</topic><topic>miR‐199a</topic><topic>Pelvic pain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maged, Ahmed M.</creatorcontrib><creatorcontrib>Deeb, Wesam S.</creatorcontrib><creatorcontrib>El Amir, Azza</creatorcontrib><creatorcontrib>Zaki, Sherif S.</creatorcontrib><creatorcontrib>El Sawah, Heba</creatorcontrib><creatorcontrib>Al Mohamady, Maged</creatorcontrib><creatorcontrib>Metwally, Ahmed A.</creatorcontrib><creatorcontrib>Katta, Maha A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of gynecology and obstetrics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maged, Ahmed M.</au><au>Deeb, Wesam S.</au><au>El Amir, Azza</au><au>Zaki, Sherif S.</au><au>El Sawah, Heba</au><au>Al Mohamady, Maged</au><au>Metwally, Ahmed A.</au><au>Katta, Maha A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis</atitle><jtitle>International journal of gynecology and obstetrics</jtitle><addtitle>Int J Gynaecol Obstet</addtitle><date>2018-04</date><risdate>2018</risdate><volume>141</volume><issue>1</issue><spage>14</spage><epage>19</epage><pages>14-19</pages><issn>0020-7292</issn><eissn>1879-3479</eissn><abstract>Objective
To evaluate the value of serum microRNA‐122 (miR‐122) and miR‐199a as reliable noninvasive biomarkers in the diagnosis of endometriosis.
Methods
During 2015–2016, at a teaching hospital in Egypt, a prospective cohort study was conducted on 45 women with pelvic endometriosis and 35 women who underwent laparoscopy for pelvic pain but were not diagnosed with endometriosis. Blood and peritoneal fluid (PF) samples were collected; interleukin‐6 (IL‐6) was detected by enzyme‐linked immunosorbent assay and miR‐122 and miR‐199a expression was measured by quantitative real‐time polymerase chain reaction.
Results
The serum and PF levels of IL‐6, miR‐122, and miR‐199a were significantly higher in women with endometriosis than in controls (P<0.001 for all comparisons). Serum miR‐122 expression was positively correlated with serum IL‐6 (r=0.597), PF IL‐6 (r=0.603), PF miR‐122 (r=0.934), serum miR‐199a (r=0.727), and PF miR‐199a (r=0.653). Serum miR‐199a expression was positively correlated with serum IL‐6 (r=0.677), PF IL‐6 (r=0.678), PF miR‐122 (r=0.744), and PF miR‐199a (r=0.932). Serum miR‐122 and miR‐199a had a sensitivity of 95.6% and 100.0%, and a specificity of 91.4% and 100%, respectively, for the detection of endometriosis.
Conclusion
Serum miR‐122 and miR‐199a were significantly increased in endometriosis, indicating that these microRNAs might serve as biomarkers for the diagnosis of endometriosis.
Serum miR‐122 and miR‐199a are significantly increased in women with endometriosis and these microRNAs have a strong potential to act as biomarkers to diagnose endometriosis.</abstract><cop>United States</cop><pmid>29149541</pmid><doi>10.1002/ijgo.12392</doi><tpages>6</tpages></addata></record> |
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| language | eng |
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| source | Wiley Journals |
| subjects | Endometriosis MicroRNA miR‐122 miR‐199a Pelvic pain |
| title | Diagnostic accuracy of serum miR‐122 and miR‐199a in women with endometriosis |
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