Characterization of oxacillin-susceptible mecA -positive Staphylococcus aureus : a new type of MRSA

Abstract Methicillin-resistant Staphylococcus aureus (MRSA) has been defined as S. aureus having the mecA gene or showing a minimum inhibitory concentration (MIC) of oxacillin higher than 4 mg/l. However, some clinical isolates are mecA- positive and oxacillin-susceptible. Therefore, we surveyed the...

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Veröffentlicht in:Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 2007-04, Vol.13 (2), p.79-86
Hauptverfasser: Hososaka, Yasuko, Hanaki, Hideaki, Endo, Harumi, Suzuki, Yumiko, Nakae, Taiji, Nagasawa, Zenzo, Otsuka, Yoshihito, Sunakawa, Keisuke
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container_title Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy
container_volume 13
creator Hososaka, Yasuko
Hanaki, Hideaki
Endo, Harumi
Suzuki, Yumiko
Nakae, Taiji
Nagasawa, Zenzo
Otsuka, Yoshihito
Sunakawa, Keisuke
description Abstract Methicillin-resistant Staphylococcus aureus (MRSA) has been defined as S. aureus having the mecA gene or showing a minimum inhibitory concentration (MIC) of oxacillin higher than 4 mg/l. However, some clinical isolates are mecA- positive and oxacillin-susceptible. Therefore, we surveyed the occurrence of S. aureus having the mecA gene and an MIC of oxacillin of less than 2 mg/l (oxacillin-susceptible MRSA; OS-MRSA) in a total of 480 strains of S. aureus collected from 11 hospitals in different location in Japan isolated from 2003 through 2005. We found 6 strains matching the criteria for OS-MRSA. All 6 strains were staphylococcal cassette chromosome (SCC) mec -positive, without exception, and 4 strains showed the SCC mec type III-variant, which is unique in Japan. These OS-MRSAs were least resistant to oxacillin among the MRSAs tested and they were within the susceptible range to seven other β-lactam antibiotics tested. Thus, OS-MRSA may become a high-resistant MRSA upon the treatment of patients with β-lactam antibiotics. To characterize whether these OS-MRSAs were hospital-acquired or community-acquired MRSAs, we tested for the presence of the genes encoding toxins. Genes encoding hemolysin, exfoliative toxin, enterotoxin, toxic shock syndrome toxin-1, and Panton-Valentine leukocidin were found in 6, 4, 0, 0, and 0 strains, respectively. These results revealed that OS-MRSAs could be classified as a new type of MRSA that exhibits properties distinguishable from either hospital- or community-acquired MRSA. Coagulase typing of the OS-MRSAs supported the above conclusion. In this study, the occurrence of OS-MRSA at a certain frequency was noted; precautions are called for in the classification of oxacillin-resistant S. aureus and in the treatment of OS-MRSA infection.
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However, some clinical isolates are mecA- positive and oxacillin-susceptible. Therefore, we surveyed the occurrence of S. aureus having the mecA gene and an MIC of oxacillin of less than 2 mg/l (oxacillin-susceptible MRSA; OS-MRSA) in a total of 480 strains of S. aureus collected from 11 hospitals in different location in Japan isolated from 2003 through 2005. We found 6 strains matching the criteria for OS-MRSA. All 6 strains were staphylococcal cassette chromosome (SCC) mec -positive, without exception, and 4 strains showed the SCC mec type III-variant, which is unique in Japan. These OS-MRSAs were least resistant to oxacillin among the MRSAs tested and they were within the susceptible range to seven other β-lactam antibiotics tested. Thus, OS-MRSA may become a high-resistant MRSA upon the treatment of patients with β-lactam antibiotics. To characterize whether these OS-MRSAs were hospital-acquired or community-acquired MRSAs, we tested for the presence of the genes encoding toxins. Genes encoding hemolysin, exfoliative toxin, enterotoxin, toxic shock syndrome toxin-1, and Panton-Valentine leukocidin were found in 6, 4, 0, 0, and 0 strains, respectively. These results revealed that OS-MRSAs could be classified as a new type of MRSA that exhibits properties distinguishable from either hospital- or community-acquired MRSA. Coagulase typing of the OS-MRSAs supported the above conclusion. 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Genes encoding hemolysin, exfoliative toxin, enterotoxin, toxic shock syndrome toxin-1, and Panton-Valentine leukocidin were found in 6, 4, 0, 0, and 0 strains, respectively. These results revealed that OS-MRSAs could be classified as a new type of MRSA that exhibits properties distinguishable from either hospital- or community-acquired MRSA. Coagulase typing of the OS-MRSAs supported the above conclusion. 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source MEDLINE; Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings
subjects Anti-Bacterial Agents - pharmacology
Bacterial Toxins - genetics
beta-Lactamases - metabolism
Community-Acquired Infections - genetics
Community-Acquired Infections - microbiology
Cross Infection - genetics
Cross Infection - microbiology
Hematology, Oncology and Palliative Medicine
Humans
Japan
Methicillin Resistance - drug effects
Methicillin Resistance - genetics
Microbial Sensitivity Tests
Oxacillin - pharmacology
Staphylococcus aureus
Staphylococcus aureus - classification
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Staphylococcus aureus - isolation & purification
title Characterization of oxacillin-susceptible mecA -positive Staphylococcus aureus : a new type of MRSA
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