Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm
Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm ( Helicoverpa armigera ) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by H. armigera remains a challenge. Toward developing a new generation of insect-resistant tr...
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| Veröffentlicht in: | Transgenic research 2017-12, Vol.26 (6), p.763-774 |
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| creator | Chen, Wen-bo Lu, Guo-qing Cheng, Hong-mei Liu, Chen-xi Xiao, Yu-tao Xu, Chao Shen, Zhi-cheng Soberón, Mario Bravo, Alejandra Wu, Kong-ming |
| description | Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (
Helicoverpa armigera
) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by
H. armigera
remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three
H. armigera
strains, one that is susceptible and two that are Cry1Ac-resistant, to determine if Vip3AcAa is a good candidate for development the pyramid cotton with Cry1Ac toxin. Our results showed that evolution of insect resistance to Cry1Ac toxin did not influence the sensitivity of Cry1Ac-resistant strains to Vip3AcAa. For the strains examined, observed mortality was equivalent to the expected mortality for all the combinations of Vip3AcAa and Cry1Ac tested, reflecting independent activity between these two toxins. When this chimeric
vip3AcAa
gene and the
cry1Ac
gene were introduced into cotton, mortality rates of Cry1Ac resistant
H. armigera
larvae strains that fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and cotton producing only Cry1Ac. These results suggest that the Vip3AcAa protein is an excellent option for a “pyramid” strategy for pest resistance management in China. |
| doi_str_mv | 10.1007/s11248-017-0048-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1965264210</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1965264210</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-e110a6c6e1360bcdafa3f645ce2e91db7d4f5d7a7e8754a9e903dc2443970dd63</originalsourceid><addsrcrecordid>eNp1kUtrHDEQhEVIiNd2fkAuYSCXXBSrpRk9jsuSFxh8cXwVWqlnIzOj2UiziX3NL4-GXYcQyKkb-qvqgiLkNbD3wJi6KgC81ZSBoozVRT8jK-iUoEZI_ZysmJGcag3mjJyXcs9YVWnxkpxxA60ApVfk1212qewwRd_4aZ6nVAfFh33GUmLaNf5bHDHX613ci7Vfu8al0GzyI6wXReoxlwb7Hv0cf2Czz9O8rNXH7VxMZT6xtBrGMrs0P_3ZTsPwc8rjJXnRu6Hgq9O8IF8_frjdfKbXN5--bNbX1AvFZ4oAzEkvEYRkWx9c70Qv284jRwNhq0Lbd0E5hVp1rTNomAiet60wioUgxQV5d_StGb8fsMx2jMXjMLiE06FYMLLjsuXAKvr2H_R-OuRU0y1UdezA8ErBkfJ5KiVjb_c5ji4_WmB2KcgeC7K1ILsUZHXVvDk5H7Yjhj-Kp0YqwI9Aqae0w_zX6_-6_gZhfZz8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1964395192</pqid></control><display><type>article</type><title>Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm</title><source>SpringerLink Journals</source><creator>Chen, Wen-bo ; Lu, Guo-qing ; Cheng, Hong-mei ; Liu, Chen-xi ; Xiao, Yu-tao ; Xu, Chao ; Shen, Zhi-cheng ; Soberón, Mario ; Bravo, Alejandra ; Wu, Kong-ming</creator><creatorcontrib>Chen, Wen-bo ; Lu, Guo-qing ; Cheng, Hong-mei ; Liu, Chen-xi ; Xiao, Yu-tao ; Xu, Chao ; Shen, Zhi-cheng ; Soberón, Mario ; Bravo, Alejandra ; Wu, Kong-ming</creatorcontrib><description>Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (
Helicoverpa armigera
) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by
H. armigera
remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three
H. armigera
strains, one that is susceptible and two that are Cry1Ac-resistant, to determine if Vip3AcAa is a good candidate for development the pyramid cotton with Cry1Ac toxin. Our results showed that evolution of insect resistance to Cry1Ac toxin did not influence the sensitivity of Cry1Ac-resistant strains to Vip3AcAa. For the strains examined, observed mortality was equivalent to the expected mortality for all the combinations of Vip3AcAa and Cry1Ac tested, reflecting independent activity between these two toxins. When this chimeric
vip3AcAa
gene and the
cry1Ac
gene were introduced into cotton, mortality rates of Cry1Ac resistant
H. armigera
larvae strains that fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and cotton producing only Cry1Ac. These results suggest that the Vip3AcAa protein is an excellent option for a “pyramid” strategy for pest resistance management in China.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-017-0048-8</identifier><identifier>PMID: 29143178</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Animal Genetics and Genomics ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Cotton ; Crop yield ; Cry1Ac gene ; Cry1Ac toxin ; Genetic Engineering ; Insecticide resistance ; Larvae ; Life Sciences ; Molecular Medicine ; Mortality ; Original Paper ; Pest resistance ; Plant Genetics and Genomics ; Plant protection ; Planting ; Transgenic plants ; Transgenics</subject><ispartof>Transgenic research, 2017-12, Vol.26 (6), p.763-774</ispartof><rights>Springer International Publishing AG 2017</rights><rights>Transgenic Research is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-e110a6c6e1360bcdafa3f645ce2e91db7d4f5d7a7e8754a9e903dc2443970dd63</citedby><cites>FETCH-LOGICAL-c372t-e110a6c6e1360bcdafa3f645ce2e91db7d4f5d7a7e8754a9e903dc2443970dd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11248-017-0048-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11248-017-0048-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29143178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Wen-bo</creatorcontrib><creatorcontrib>Lu, Guo-qing</creatorcontrib><creatorcontrib>Cheng, Hong-mei</creatorcontrib><creatorcontrib>Liu, Chen-xi</creatorcontrib><creatorcontrib>Xiao, Yu-tao</creatorcontrib><creatorcontrib>Xu, Chao</creatorcontrib><creatorcontrib>Shen, Zhi-cheng</creatorcontrib><creatorcontrib>Soberón, Mario</creatorcontrib><creatorcontrib>Bravo, Alejandra</creatorcontrib><creatorcontrib>Wu, Kong-ming</creatorcontrib><title>Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><addtitle>Transgenic Res</addtitle><description>Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (
Helicoverpa armigera
) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by
H. armigera
remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three
H. armigera
strains, one that is susceptible and two that are Cry1Ac-resistant, to determine if Vip3AcAa is a good candidate for development the pyramid cotton with Cry1Ac toxin. Our results showed that evolution of insect resistance to Cry1Ac toxin did not influence the sensitivity of Cry1Ac-resistant strains to Vip3AcAa. For the strains examined, observed mortality was equivalent to the expected mortality for all the combinations of Vip3AcAa and Cry1Ac tested, reflecting independent activity between these two toxins. When this chimeric
vip3AcAa
gene and the
cry1Ac
gene were introduced into cotton, mortality rates of Cry1Ac resistant
H. armigera
larvae strains that fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and cotton producing only Cry1Ac. These results suggest that the Vip3AcAa protein is an excellent option for a “pyramid” strategy for pest resistance management in China.</description><subject>Animal Genetics and Genomics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Cotton</subject><subject>Crop yield</subject><subject>Cry1Ac gene</subject><subject>Cry1Ac toxin</subject><subject>Genetic Engineering</subject><subject>Insecticide resistance</subject><subject>Larvae</subject><subject>Life Sciences</subject><subject>Molecular Medicine</subject><subject>Mortality</subject><subject>Original Paper</subject><subject>Pest resistance</subject><subject>Plant Genetics and Genomics</subject><subject>Plant protection</subject><subject>Planting</subject><subject>Transgenic plants</subject><subject>Transgenics</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUtrHDEQhEVIiNd2fkAuYSCXXBSrpRk9jsuSFxh8cXwVWqlnIzOj2UiziX3NL4-GXYcQyKkb-qvqgiLkNbD3wJi6KgC81ZSBoozVRT8jK-iUoEZI_ZysmJGcag3mjJyXcs9YVWnxkpxxA60ApVfk1212qewwRd_4aZ6nVAfFh33GUmLaNf5bHDHX613ci7Vfu8al0GzyI6wXReoxlwb7Hv0cf2Czz9O8rNXH7VxMZT6xtBrGMrs0P_3ZTsPwc8rjJXnRu6Hgq9O8IF8_frjdfKbXN5--bNbX1AvFZ4oAzEkvEYRkWx9c70Qv284jRwNhq0Lbd0E5hVp1rTNomAiet60wioUgxQV5d_StGb8fsMx2jMXjMLiE06FYMLLjsuXAKvr2H_R-OuRU0y1UdezA8ErBkfJ5KiVjb_c5ji4_WmB2KcgeC7K1ILsUZHXVvDk5H7Yjhj-Kp0YqwI9Aqae0w_zX6_-6_gZhfZz8</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Chen, Wen-bo</creator><creator>Lu, Guo-qing</creator><creator>Cheng, Hong-mei</creator><creator>Liu, Chen-xi</creator><creator>Xiao, Yu-tao</creator><creator>Xu, Chao</creator><creator>Shen, Zhi-cheng</creator><creator>Soberón, Mario</creator><creator>Bravo, Alejandra</creator><creator>Wu, Kong-ming</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm</title><author>Chen, Wen-bo ; Lu, Guo-qing ; Cheng, Hong-mei ; Liu, Chen-xi ; Xiao, Yu-tao ; Xu, Chao ; Shen, Zhi-cheng ; Soberón, Mario ; Bravo, Alejandra ; Wu, Kong-ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-e110a6c6e1360bcdafa3f645ce2e91db7d4f5d7a7e8754a9e903dc2443970dd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Animal Genetics and Genomics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Cotton</topic><topic>Crop yield</topic><topic>Cry1Ac gene</topic><topic>Cry1Ac toxin</topic><topic>Genetic Engineering</topic><topic>Insecticide resistance</topic><topic>Larvae</topic><topic>Life Sciences</topic><topic>Molecular Medicine</topic><topic>Mortality</topic><topic>Original Paper</topic><topic>Pest resistance</topic><topic>Plant Genetics and Genomics</topic><topic>Plant protection</topic><topic>Planting</topic><topic>Transgenic plants</topic><topic>Transgenics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Wen-bo</creatorcontrib><creatorcontrib>Lu, Guo-qing</creatorcontrib><creatorcontrib>Cheng, Hong-mei</creatorcontrib><creatorcontrib>Liu, Chen-xi</creatorcontrib><creatorcontrib>Xiao, Yu-tao</creatorcontrib><creatorcontrib>Xu, Chao</creatorcontrib><creatorcontrib>Shen, Zhi-cheng</creatorcontrib><creatorcontrib>Soberón, Mario</creatorcontrib><creatorcontrib>Bravo, Alejandra</creatorcontrib><creatorcontrib>Wu, Kong-ming</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Wen-bo</au><au>Lu, Guo-qing</au><au>Cheng, Hong-mei</au><au>Liu, Chen-xi</au><au>Xiao, Yu-tao</au><au>Xu, Chao</au><au>Shen, Zhi-cheng</au><au>Soberón, Mario</au><au>Bravo, Alejandra</au><au>Wu, Kong-ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm</atitle><jtitle>Transgenic research</jtitle><stitle>Transgenic Res</stitle><addtitle>Transgenic Res</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>26</volume><issue>6</issue><spage>763</spage><epage>774</epage><pages>763-774</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Wide planting of transgenic Bt cotton in China since 1997 to control cotton bollworm (
Helicoverpa armigera
) has increased yields and decreased insecticide use, but the evolution of resistance to Bt cotton by
H. armigera
remains a challenge. Toward developing a new generation of insect-resistant transgenic crops, a chimeric protein of Vip3Aa1 and Vip3Ac1, named Vip3AcAa, having a broader insecticidal spectrum, was specifically created previously in our laboratory. In this study, we investigated cross resistance and interactions between Vip3AcAa and Cry1Ac with three
H. armigera
strains, one that is susceptible and two that are Cry1Ac-resistant, to determine if Vip3AcAa is a good candidate for development the pyramid cotton with Cry1Ac toxin. Our results showed that evolution of insect resistance to Cry1Ac toxin did not influence the sensitivity of Cry1Ac-resistant strains to Vip3AcAa. For the strains examined, observed mortality was equivalent to the expected mortality for all the combinations of Vip3AcAa and Cry1Ac tested, reflecting independent activity between these two toxins. When this chimeric
vip3AcAa
gene and the
cry1Ac
gene were introduced into cotton, mortality rates of Cry1Ac resistant
H. armigera
larvae strains that fed on this new cotton increased significantly compared with larvae fed on non-Bt cotton and cotton producing only Cry1Ac. These results suggest that the Vip3AcAa protein is an excellent option for a “pyramid” strategy for pest resistance management in China.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>29143178</pmid><doi>10.1007/s11248-017-0048-8</doi><tpages>12</tpages></addata></record> |
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| ispartof | Transgenic research, 2017-12, Vol.26 (6), p.763-774 |
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| subjects | Animal Genetics and Genomics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Cotton Crop yield Cry1Ac gene Cry1Ac toxin Genetic Engineering Insecticide resistance Larvae Life Sciences Molecular Medicine Mortality Original Paper Pest resistance Plant Genetics and Genomics Plant protection Planting Transgenic plants Transgenics |
| title | Transgenic cotton co-expressing chimeric Vip3AcAa and Cry1Ac confers effective protection against Cry1Ac-resistant cotton bollworm |
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