Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus
Cynara cardunculus : L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of...
Gespeichert in:
| Veröffentlicht in: | Molecular genetics and genomics : MGG 2018-04, Vol.293 (2), p.417-433 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 433 |
|---|---|
| container_issue | 2 |
| container_start_page | 417 |
| container_title | Molecular genetics and genomics : MGG |
| container_volume | 293 |
| creator | Ferro, Ana Margarida Ramos, Patrícia Guerra, Ângela Parreira, Paula Brás, Teresa Guerreiro, Olinda Jerónimo, Eliana Capel, Carmen Capel, Juan Yuste-Lisbona, Fernando J. Duarte, Maria F. Lozano, Rafael Oliveira, M. Margarida Gonçalves, Sónia |
| description | Cynara cardunculus
: L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of this work was to screen a collection of
C. cardunculus
, from different origins, for new allelic variants in
germacrene A synthase
(
GAS
) gene involved in the cynaropicrin biosynthesis and correlate them with improved cynaropicrin content and biological activities. Using high-resolution melting, nine haplotypes were identified. The putative impact of the identified allelic variants in GAS protein was evaluated by bioinformatic tools and polymorphisms that putatively lead to protein conformational changes were described. Additionally, cynaropicrin and main pentacyclic triterpenes contents, and antithrombin, antimicrobial and antiproliferative activities were also determined in
C. cardunculus
leaf lipophilic-derived extracts. In this work we identified allelic variants with putative impact on GAS protein, which are significantly associated with cynaropicrin content and antiproliferative activity. The results obtained suggest that the identified polymorphisms should be explored as putative genetic markers correlated with biological properties in
Cynara cardunculus
. |
| doi_str_mv | 10.1007/s00438-017-1388-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1965248902</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1964910956</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-13443e96491fc7d6774c75871d565babcb66b3f524c6ba3577dda3df2690ee6a3</originalsourceid><addsrcrecordid>eNp1kctu3CAUhlGVqrm0D9BNhdRNN07B2GAvo1HaRIqUTbtGxxhniDzgcnArz0P0mYMzaRRVyorb958DfIR85OycM6a-ImOVaArGVcFF0xT7N-SEy7yqZCmOnue8PianiPcsg7JU78hx2fIclPKE_L2CaQxpmSwFD-OCDmkYaNpaemfjDky03tILiotPW8B1169oTwExGAfJBU__uLSlZvEQw-RMdJ6a4JP16ZHsXBjDnTMwUjDJ_XbJWaQZ2qwJoAZiP3szjzO-J28HGNF-eBrPyM9vlz82V8XN7ffrzcVNYYQqU35tVQnbyqrlg1G9VKoyqm4U72tZd9CZTspODHVZGdmBqJXqexD9UMqWWStBnJEvh7pTDL9mi0nvHBo7juBtmFHzVuZw07Iyo5__Q-_DHPNfPVL5BqytZab4gTIxIEY76Cm6HcRFc6ZXWfogS2cHepWl9znz6any3O1s_5z4ZycD5QHAfOSzjxetX636AFPxoqY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1964910956</pqid></control><display><type>article</type><title>Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus</title><source>SpringerLink Journals - AutoHoldings</source><creator>Ferro, Ana Margarida ; Ramos, Patrícia ; Guerra, Ângela ; Parreira, Paula ; Brás, Teresa ; Guerreiro, Olinda ; Jerónimo, Eliana ; Capel, Carmen ; Capel, Juan ; Yuste-Lisbona, Fernando J. ; Duarte, Maria F. ; Lozano, Rafael ; Oliveira, M. Margarida ; Gonçalves, Sónia</creator><creatorcontrib>Ferro, Ana Margarida ; Ramos, Patrícia ; Guerra, Ângela ; Parreira, Paula ; Brás, Teresa ; Guerreiro, Olinda ; Jerónimo, Eliana ; Capel, Carmen ; Capel, Juan ; Yuste-Lisbona, Fernando J. ; Duarte, Maria F. ; Lozano, Rafael ; Oliveira, M. Margarida ; Gonçalves, Sónia</creatorcontrib><description>Cynara cardunculus
: L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of this work was to screen a collection of
C. cardunculus
, from different origins, for new allelic variants in
germacrene A synthase
(
GAS
) gene involved in the cynaropicrin biosynthesis and correlate them with improved cynaropicrin content and biological activities. Using high-resolution melting, nine haplotypes were identified. The putative impact of the identified allelic variants in GAS protein was evaluated by bioinformatic tools and polymorphisms that putatively lead to protein conformational changes were described. Additionally, cynaropicrin and main pentacyclic triterpenes contents, and antithrombin, antimicrobial and antiproliferative activities were also determined in
C. cardunculus
leaf lipophilic-derived extracts. In this work we identified allelic variants with putative impact on GAS protein, which are significantly associated with cynaropicrin content and antiproliferative activity. The results obtained suggest that the identified polymorphisms should be explored as putative genetic markers correlated with biological properties in
Cynara cardunculus
.</description><identifier>ISSN: 1617-4615</identifier><identifier>EISSN: 1617-4623</identifier><identifier>DOI: 10.1007/s00438-017-1388-z</identifier><identifier>PMID: 29143866</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animal Genetics and Genomics ; Antithrombin ; Biochemistry ; Biomarkers ; Biomedical and Life Sciences ; Cancer ; Cynara cardunculus ; Cytotoxicity ; Genetic markers ; Germacrene ; Haplotypes ; Human Genetics ; Hyperlipidemia ; Life Sciences ; Lipophilic ; Microbial Genetics and Genomics ; Muscle relaxants ; Original Article ; Parasympathetic nervous system ; Plant Genetics and Genomics ; Proteins ; Terpenic compounds ; Triterpenes</subject><ispartof>Molecular genetics and genomics : MGG, 2018-04, Vol.293 (2), p.417-433</ispartof><rights>Springer-Verlag GmbH Germany 2017</rights><rights>Molecular Genetics and Genomics is a copyright of Springer, (2017). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-13443e96491fc7d6774c75871d565babcb66b3f524c6ba3577dda3df2690ee6a3</citedby><cites>FETCH-LOGICAL-c372t-13443e96491fc7d6774c75871d565babcb66b3f524c6ba3577dda3df2690ee6a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00438-017-1388-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00438-017-1388-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27922,27923,41486,42555,51317</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29143866$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ferro, Ana Margarida</creatorcontrib><creatorcontrib>Ramos, Patrícia</creatorcontrib><creatorcontrib>Guerra, Ângela</creatorcontrib><creatorcontrib>Parreira, Paula</creatorcontrib><creatorcontrib>Brás, Teresa</creatorcontrib><creatorcontrib>Guerreiro, Olinda</creatorcontrib><creatorcontrib>Jerónimo, Eliana</creatorcontrib><creatorcontrib>Capel, Carmen</creatorcontrib><creatorcontrib>Capel, Juan</creatorcontrib><creatorcontrib>Yuste-Lisbona, Fernando J.</creatorcontrib><creatorcontrib>Duarte, Maria F.</creatorcontrib><creatorcontrib>Lozano, Rafael</creatorcontrib><creatorcontrib>Oliveira, M. Margarida</creatorcontrib><creatorcontrib>Gonçalves, Sónia</creatorcontrib><title>Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus</title><title>Molecular genetics and genomics : MGG</title><addtitle>Mol Genet Genomics</addtitle><addtitle>Mol Genet Genomics</addtitle><description>Cynara cardunculus
: L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of this work was to screen a collection of
C. cardunculus
, from different origins, for new allelic variants in
germacrene A synthase
(
GAS
) gene involved in the cynaropicrin biosynthesis and correlate them with improved cynaropicrin content and biological activities. Using high-resolution melting, nine haplotypes were identified. The putative impact of the identified allelic variants in GAS protein was evaluated by bioinformatic tools and polymorphisms that putatively lead to protein conformational changes were described. Additionally, cynaropicrin and main pentacyclic triterpenes contents, and antithrombin, antimicrobial and antiproliferative activities were also determined in
C. cardunculus
leaf lipophilic-derived extracts. In this work we identified allelic variants with putative impact on GAS protein, which are significantly associated with cynaropicrin content and antiproliferative activity. The results obtained suggest that the identified polymorphisms should be explored as putative genetic markers correlated with biological properties in
Cynara cardunculus
.</description><subject>Animal Genetics and Genomics</subject><subject>Antithrombin</subject><subject>Biochemistry</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer</subject><subject>Cynara cardunculus</subject><subject>Cytotoxicity</subject><subject>Genetic markers</subject><subject>Germacrene</subject><subject>Haplotypes</subject><subject>Human Genetics</subject><subject>Hyperlipidemia</subject><subject>Life Sciences</subject><subject>Lipophilic</subject><subject>Microbial Genetics and Genomics</subject><subject>Muscle relaxants</subject><subject>Original Article</subject><subject>Parasympathetic nervous system</subject><subject>Plant Genetics and Genomics</subject><subject>Proteins</subject><subject>Terpenic compounds</subject><subject>Triterpenes</subject><issn>1617-4615</issn><issn>1617-4623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kctu3CAUhlGVqrm0D9BNhdRNN07B2GAvo1HaRIqUTbtGxxhniDzgcnArz0P0mYMzaRRVyorb958DfIR85OycM6a-ImOVaArGVcFF0xT7N-SEy7yqZCmOnue8PianiPcsg7JU78hx2fIclPKE_L2CaQxpmSwFD-OCDmkYaNpaemfjDky03tILiotPW8B1169oTwExGAfJBU__uLSlZvEQw-RMdJ6a4JP16ZHsXBjDnTMwUjDJ_XbJWaQZ2qwJoAZiP3szjzO-J28HGNF-eBrPyM9vlz82V8XN7ffrzcVNYYQqU35tVQnbyqrlg1G9VKoyqm4U72tZd9CZTspODHVZGdmBqJXqexD9UMqWWStBnJEvh7pTDL9mi0nvHBo7juBtmFHzVuZw07Iyo5__Q-_DHPNfPVL5BqytZab4gTIxIEY76Cm6HcRFc6ZXWfogS2cHepWl9znz6any3O1s_5z4ZycD5QHAfOSzjxetX636AFPxoqY</recordid><startdate>20180401</startdate><enddate>20180401</enddate><creator>Ferro, Ana Margarida</creator><creator>Ramos, Patrícia</creator><creator>Guerra, Ângela</creator><creator>Parreira, Paula</creator><creator>Brás, Teresa</creator><creator>Guerreiro, Olinda</creator><creator>Jerónimo, Eliana</creator><creator>Capel, Carmen</creator><creator>Capel, Juan</creator><creator>Yuste-Lisbona, Fernando J.</creator><creator>Duarte, Maria F.</creator><creator>Lozano, Rafael</creator><creator>Oliveira, M. Margarida</creator><creator>Gonçalves, Sónia</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20180401</creationdate><title>Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus</title><author>Ferro, Ana Margarida ; Ramos, Patrícia ; Guerra, Ângela ; Parreira, Paula ; Brás, Teresa ; Guerreiro, Olinda ; Jerónimo, Eliana ; Capel, Carmen ; Capel, Juan ; Yuste-Lisbona, Fernando J. ; Duarte, Maria F. ; Lozano, Rafael ; Oliveira, M. Margarida ; Gonçalves, Sónia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-13443e96491fc7d6774c75871d565babcb66b3f524c6ba3577dda3df2690ee6a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Genetics and Genomics</topic><topic>Antithrombin</topic><topic>Biochemistry</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Cancer</topic><topic>Cynara cardunculus</topic><topic>Cytotoxicity</topic><topic>Genetic markers</topic><topic>Germacrene</topic><topic>Haplotypes</topic><topic>Human Genetics</topic><topic>Hyperlipidemia</topic><topic>Life Sciences</topic><topic>Lipophilic</topic><topic>Microbial Genetics and Genomics</topic><topic>Muscle relaxants</topic><topic>Original Article</topic><topic>Parasympathetic nervous system</topic><topic>Plant Genetics and Genomics</topic><topic>Proteins</topic><topic>Terpenic compounds</topic><topic>Triterpenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferro, Ana Margarida</creatorcontrib><creatorcontrib>Ramos, Patrícia</creatorcontrib><creatorcontrib>Guerra, Ângela</creatorcontrib><creatorcontrib>Parreira, Paula</creatorcontrib><creatorcontrib>Brás, Teresa</creatorcontrib><creatorcontrib>Guerreiro, Olinda</creatorcontrib><creatorcontrib>Jerónimo, Eliana</creatorcontrib><creatorcontrib>Capel, Carmen</creatorcontrib><creatorcontrib>Capel, Juan</creatorcontrib><creatorcontrib>Yuste-Lisbona, Fernando J.</creatorcontrib><creatorcontrib>Duarte, Maria F.</creatorcontrib><creatorcontrib>Lozano, Rafael</creatorcontrib><creatorcontrib>Oliveira, M. Margarida</creatorcontrib><creatorcontrib>Gonçalves, Sónia</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular genetics and genomics : MGG</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferro, Ana Margarida</au><au>Ramos, Patrícia</au><au>Guerra, Ângela</au><au>Parreira, Paula</au><au>Brás, Teresa</au><au>Guerreiro, Olinda</au><au>Jerónimo, Eliana</au><au>Capel, Carmen</au><au>Capel, Juan</au><au>Yuste-Lisbona, Fernando J.</au><au>Duarte, Maria F.</au><au>Lozano, Rafael</au><au>Oliveira, M. Margarida</au><au>Gonçalves, Sónia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus</atitle><jtitle>Molecular genetics and genomics : MGG</jtitle><stitle>Mol Genet Genomics</stitle><addtitle>Mol Genet Genomics</addtitle><date>2018-04-01</date><risdate>2018</risdate><volume>293</volume><issue>2</issue><spage>417</spage><epage>433</epage><pages>417-433</pages><issn>1617-4615</issn><eissn>1617-4623</eissn><abstract>Cynara cardunculus
: L. represents a natural source of terpenic compounds, with the predominant molecule being cynaropicrin. Cynaropicrin is gaining interest since it has been correlated to anti-hyperlipidaemia, antispasmodic and cytotoxicity activity against leukocyte cancer cells. The objective of this work was to screen a collection of
C. cardunculus
, from different origins, for new allelic variants in
germacrene A synthase
(
GAS
) gene involved in the cynaropicrin biosynthesis and correlate them with improved cynaropicrin content and biological activities. Using high-resolution melting, nine haplotypes were identified. The putative impact of the identified allelic variants in GAS protein was evaluated by bioinformatic tools and polymorphisms that putatively lead to protein conformational changes were described. Additionally, cynaropicrin and main pentacyclic triterpenes contents, and antithrombin, antimicrobial and antiproliferative activities were also determined in
C. cardunculus
leaf lipophilic-derived extracts. In this work we identified allelic variants with putative impact on GAS protein, which are significantly associated with cynaropicrin content and antiproliferative activity. The results obtained suggest that the identified polymorphisms should be explored as putative genetic markers correlated with biological properties in
Cynara cardunculus
.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>29143866</pmid><doi>10.1007/s00438-017-1388-z</doi><tpages>17</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1617-4615 |
| ispartof | Molecular genetics and genomics : MGG, 2018-04, Vol.293 (2), p.417-433 |
| issn | 1617-4615 1617-4623 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1965248902 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Animal Genetics and Genomics Antithrombin Biochemistry Biomarkers Biomedical and Life Sciences Cancer Cynara cardunculus Cytotoxicity Genetic markers Germacrene Haplotypes Human Genetics Hyperlipidemia Life Sciences Lipophilic Microbial Genetics and Genomics Muscle relaxants Original Article Parasympathetic nervous system Plant Genetics and Genomics Proteins Terpenic compounds Triterpenes |
| title | Haplotype analysis of the germacrene A synthase gene and association with cynaropicrin content and biological activities in Cynara cardunculus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T07%3A07%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Haplotype%20analysis%20of%20the%20germacrene%20A%20synthase%20gene%20and%20association%20with%20cynaropicrin%20content%20and%20biological%20activities%20in%20Cynara%20cardunculus&rft.jtitle=Molecular%20genetics%20and%20genomics%20:%20MGG&rft.au=Ferro,%20Ana%20Margarida&rft.date=2018-04-01&rft.volume=293&rft.issue=2&rft.spage=417&rft.epage=433&rft.pages=417-433&rft.issn=1617-4615&rft.eissn=1617-4623&rft_id=info:doi/10.1007/s00438-017-1388-z&rft_dat=%3Cproquest_cross%3E1964910956%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1964910956&rft_id=info:pmid/29143866&rfr_iscdi=true |