Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase

The possibility to sequence cytotoxic O 6 -alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which m...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2017-11, Vol.53 (94), p.1274-1277
Hauptverfasser:	Betz, K, Nilforoushan, A, Wyss, L. A, Diederichs, K, Sturla, S. J, Marx, A
Format:	Artikel
Sprache:	eng
Schlagworte:	Adducts Deoxyribonucleic acid DNA DNA polymerase Gene sequencing Structural damage
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

container_end_page	1277
container_issue	94
container_start_page	1274
container_title	Chemical communications (Cambridge, England)
container_volume	53
creator	Betz, K Nilforoushan, A Wyss, L. A Diederichs, K Sturla, S. J Marx, A
description	The possibility to sequence cytotoxic O 6 -alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing. The structural basis for selective incorporation of BenziMP opposite O 6 -MeG by KlenTaq DNA polymerase is elucidated by X-ray crystallography.
doi_str_mv	10.1039/c7cc07173f
format	Article
fullrecord	<record><control><sourceid>proquest_rsc_p</sourceid><recordid>TN_cdi_proquest_miscellaneous_1964700596</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1964700596</sourcerecordid><originalsourceid>FETCH-LOGICAL-c337t-32df45ac459751732fd96936927261aeec1d50d2c92ae5d4bb52052dc6e159f73</originalsourceid><addsrcrecordid>eNpdkU1r3DAQhkVpaT6aS-8pglxCwKk-LGt1XNwmKSzNIQnkZmRpRBS8livJgf330Wa3KVSX0TDPvMzMi9BXSi4p4eq7kcYQSSV3H9Ah5U1diXrx-HH7F6qSvBYH6CilZ1IeFYvP6ICpghHJDtHLXY6zyXPUA-518gm7EHF-ApxgAJP9C2A_mhCnEHX2YcTBYT1iHbN33vjSNs5mgJC9BRymKSSfAWv84_cSa2uLNu43-3wKw2YNUSf4gj45PSQ42cdj9HD18769qVa317_a5aoynMtccWZdLbSphZKiLMicVY3ijWKSNVQDGGoFscwopkHYuu8FI4JZ0wAVykl-jM53ulMMf2ZIuVv7ZGAY9AhhTh1VTS0JEaop6Nl_6HOY41im6xihZME5a-pCXewoE0NKEVw3Rb_WcdNR0m3d6FrZtm9uXBX4215y7tdg39G_5y_A6Q6IybxX_9nJXwHIu45v</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2010833264</pqid></control><display><type>article</type><title>Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase</title><source>Royal Society Of Chemistry Journals 2008-</source><source>Alma/SFX Local Collection</source><creator>Betz, K ; Nilforoushan, A ; Wyss, L. A ; Diederichs, K ; Sturla, S. J ; Marx, A</creator><creatorcontrib>Betz, K ; Nilforoushan, A ; Wyss, L. A ; Diederichs, K ; Sturla, S. J ; Marx, A</creatorcontrib><description>The possibility to sequence cytotoxic O 6 -alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing. The structural basis for selective incorporation of BenziMP opposite O 6 -MeG by KlenTaq DNA polymerase is elucidated by X-ray crystallography.</description><identifier>ISSN: 1359-7345</identifier><identifier>EISSN: 1364-548X</identifier><identifier>DOI: 10.1039/c7cc07173f</identifier><identifier>PMID: 29136072</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Adducts ; Deoxyribonucleic acid ; DNA ; DNA polymerase ; Gene sequencing ; Structural damage</subject><ispartof>Chemical communications (Cambridge, England), 2017-11, Vol.53 (94), p.1274-1277</ispartof><rights>Copyright Royal Society of Chemistry 2017</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c337t-32df45ac459751732fd96936927261aeec1d50d2c92ae5d4bb52052dc6e159f73</citedby><cites>FETCH-LOGICAL-c337t-32df45ac459751732fd96936927261aeec1d50d2c92ae5d4bb52052dc6e159f73</cites><orcidid>0000-0002-6471-3689 ; 0000-0001-6808-5950</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29136072$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Betz, K</creatorcontrib><creatorcontrib>Nilforoushan, A</creatorcontrib><creatorcontrib>Wyss, L. A</creatorcontrib><creatorcontrib>Diederichs, K</creatorcontrib><creatorcontrib>Sturla, S. J</creatorcontrib><creatorcontrib>Marx, A</creatorcontrib><title>Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase</title><title>Chemical communications (Cambridge, England)</title><addtitle>Chem Commun (Camb)</addtitle><description>The possibility to sequence cytotoxic O 6 -alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing. The structural basis for selective incorporation of BenziMP opposite O 6 -MeG by KlenTaq DNA polymerase is elucidated by X-ray crystallography.</description><subject>Adducts</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA polymerase</subject><subject>Gene sequencing</subject><subject>Structural damage</subject><issn>1359-7345</issn><issn>1364-548X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNpdkU1r3DAQhkVpaT6aS-8pglxCwKk-LGt1XNwmKSzNIQnkZmRpRBS8livJgf330Wa3KVSX0TDPvMzMi9BXSi4p4eq7kcYQSSV3H9Ah5U1diXrx-HH7F6qSvBYH6CilZ1IeFYvP6ICpghHJDtHLXY6zyXPUA-518gm7EHF-ApxgAJP9C2A_mhCnEHX2YcTBYT1iHbN33vjSNs5mgJC9BRymKSSfAWv84_cSa2uLNu43-3wKw2YNUSf4gj45PSQ42cdj9HD18769qVa317_a5aoynMtccWZdLbSphZKiLMicVY3ijWKSNVQDGGoFscwopkHYuu8FI4JZ0wAVykl-jM53ulMMf2ZIuVv7ZGAY9AhhTh1VTS0JEaop6Nl_6HOY41im6xihZME5a-pCXewoE0NKEVw3Rb_WcdNR0m3d6FrZtm9uXBX4215y7tdg39G_5y_A6Q6IybxX_9nJXwHIu45v</recordid><startdate>20171123</startdate><enddate>20171123</enddate><creator>Betz, K</creator><creator>Nilforoushan, A</creator><creator>Wyss, L. A</creator><creator>Diederichs, K</creator><creator>Sturla, S. J</creator><creator>Marx, A</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-6471-3689</orcidid><orcidid>https://orcid.org/0000-0001-6808-5950</orcidid></search><sort><creationdate>20171123</creationdate><title>Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase</title><author>Betz, K ; Nilforoushan, A ; Wyss, L. A ; Diederichs, K ; Sturla, S. J ; Marx, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c337t-32df45ac459751732fd96936927261aeec1d50d2c92ae5d4bb52052dc6e159f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adducts</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA polymerase</topic><topic>Gene sequencing</topic><topic>Structural damage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Betz, K</creatorcontrib><creatorcontrib>Nilforoushan, A</creatorcontrib><creatorcontrib>Wyss, L. A</creatorcontrib><creatorcontrib>Diederichs, K</creatorcontrib><creatorcontrib>Sturla, S. J</creatorcontrib><creatorcontrib>Marx, A</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Chemical communications (Cambridge, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Betz, K</au><au>Nilforoushan, A</au><au>Wyss, L. A</au><au>Diederichs, K</au><au>Sturla, S. J</au><au>Marx, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase</atitle><jtitle>Chemical communications (Cambridge, England)</jtitle><addtitle>Chem Commun (Camb)</addtitle><date>2017-11-23</date><risdate>2017</risdate><volume>53</volume><issue>94</issue><spage>1274</spage><epage>1277</epage><pages>1274-1277</pages><issn>1359-7345</issn><eissn>1364-548X</eissn><abstract>The possibility to sequence cytotoxic O 6 -alkylG DNA adducts would greatly benefit research. Recently we reported a benzimidazole-derived nucleotide that is selectively incorporated opposite the damaged site by a mutated DNA polymerase. Here we provide the structural basis for this reaction which may spur future developments in DNA damage sequencing. The structural basis for selective incorporation of BenziMP opposite O 6 -MeG by KlenTaq DNA polymerase is elucidated by X-ray crystallography.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>29136072</pmid><doi>10.1039/c7cc07173f</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0002-6471-3689</orcidid><orcidid>https://orcid.org/0000-0001-6808-5950</orcidid></addata></record>
fulltext	fulltext
identifier	ISSN: 1359-7345
ispartof	Chemical communications (Cambridge, England), 2017-11, Vol.53 (94), p.1274-1277
issn	1359-7345 1364-548X
language	eng
recordid	cdi_proquest_miscellaneous_1964700596
source	Royal Society Of Chemistry Journals 2008-; Alma/SFX Local Collection
subjects	Adducts Deoxyribonucleic acid DNA DNA polymerase Gene sequencing Structural damage
title	Structural basis for the selective incorporation of an artificial nucleotide opposite a DNA adduct by a DNA polymerase
url	https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T18%3A02%3A40IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_rsc_p&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Structural%20basis%20for%20the%20selective%20incorporation%20of%20an%20artificial%20nucleotide%20opposite%20a%20DNA%20adduct%20by%20a%20DNA%20polymerase&rft.jtitle=Chemical%20communications%20(Cambridge,%20England)&rft.au=Betz,%20K&rft.date=2017-11-23&rft.volume=53&rft.issue=94&rft.spage=1274&rft.epage=1277&rft.pages=1274-1277&rft.issn=1359-7345&rft.eissn=1364-548X&rft_id=info:doi/10.1039/c7cc07173f&rft_dat=%3Cproquest_rsc_p%3E1964700596%3C/proquest_rsc_p%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2010833264&rft_id=info:pmid/29136072&rfr_iscdi=true