Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District - South Africa
The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea....
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| Veröffentlicht in: | Journal of applied microbiology 2008-08, Vol.105 (2), p.424-431 |
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| description | The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. Culture-based and polymerase chain reaction techniques were used to identify E. coli O157:H7. Thirty-five per cent of meat products, 25·5% of water, 21·7% of vegetables as well as 56·5% and 43·5% of stools of confirmed and non-confirmed HIV/AIDS patients, respectively, were presumptively positive with E. coli O157. Molecular results indicated that 10·3%, 8·6% and 7·8% of the vegetables, water and meat products examined carried E. coli O157:H7, which had homologous fliCH₇, rfbEO₁₅₇ and eaeA genetic loci to the genes of some E. coli O157:H7 isolated from 12·2% and 8·8% of the stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Water, meat and meat products and vegetables are potential sources of E. coli O157:H7 that are potentially capable of causing diarrhoea in humans especially HIV/AIDS patients. Great care should be exercised to ensure that water and foods consumed by HIV/AIDS patients are safe, as contaminated water and foods can cause secondary infections in these patients. |
| doi_str_mv | 10.1111/j.1365-2672.2008.03756.x |
| format | Article |
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Culture-based and polymerase chain reaction techniques were used to identify E. coli O157:H7. Thirty-five per cent of meat products, 25·5% of water, 21·7% of vegetables as well as 56·5% and 43·5% of stools of confirmed and non-confirmed HIV/AIDS patients, respectively, were presumptively positive with E. coli O157. Molecular results indicated that 10·3%, 8·6% and 7·8% of the vegetables, water and meat products examined carried E. coli O157:H7, which had homologous fliCH₇, rfbEO₁₅₇ and eaeA genetic loci to the genes of some E. coli O157:H7 isolated from 12·2% and 8·8% of the stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Water, meat and meat products and vegetables are potential sources of E. coli O157:H7 that are potentially capable of causing diarrhoea in humans especially HIV/AIDS patients. Great care should be exercised to ensure that water and foods consumed by HIV/AIDS patients are safe, as contaminated water and foods can cause secondary infections in these patients.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2008.03756.x</identifier><identifier>PMID: 18298530</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Animals ; Colony Count, Microbial ; Diarrhea - microbiology ; Diarrhea - virology ; drinking water ; E. coli O157:H7 ; Environmental Microbiology ; Escherichia coli Infections - epidemiology ; Escherichia coli Infections - microbiology ; Escherichia coli O157 - genetics ; Escherichia coli O157 - isolation & purification ; Escherichia coli O157:H7 ; Feces - microbiology ; Food Microbiology ; Genes, Bacterial ; HIV Infections - complications ; HIV/AIDS ; Human immunodeficiency virus ; Humans ; Meat ; meat and vegetables ; PCR ; Polymerase Chain Reaction - methods ; potential link ; Prevalence ; South Africa ; stool specimens ; Vegetables ; Water Microbiology ; Water Supply</subject><ispartof>Journal of applied microbiology, 2008-08, Vol.105 (2), p.424-431</ispartof><rights>2008 The Authors. Journal compilation © 2008 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2008.03756.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2008.03756.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18298530$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abong'o, B.O</creatorcontrib><creatorcontrib>Momba, M.N.B</creatorcontrib><title>Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District - South Africa</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. Culture-based and polymerase chain reaction techniques were used to identify E. coli O157:H7. Thirty-five per cent of meat products, 25·5% of water, 21·7% of vegetables as well as 56·5% and 43·5% of stools of confirmed and non-confirmed HIV/AIDS patients, respectively, were presumptively positive with E. coli O157. Molecular results indicated that 10·3%, 8·6% and 7·8% of the vegetables, water and meat products examined carried E. coli O157:H7, which had homologous fliCH₇, rfbEO₁₅₇ and eaeA genetic loci to the genes of some E. coli O157:H7 isolated from 12·2% and 8·8% of the stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Water, meat and meat products and vegetables are potential sources of E. coli O157:H7 that are potentially capable of causing diarrhoea in humans especially HIV/AIDS patients. Great care should be exercised to ensure that water and foods consumed by HIV/AIDS patients are safe, as contaminated water and foods can cause secondary infections in these patients.</description><subject>Animals</subject><subject>Colony Count, Microbial</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhea - virology</subject><subject>drinking water</subject><subject>E. coli O157:H7</subject><subject>Environmental Microbiology</subject><subject>Escherichia coli Infections - epidemiology</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli O157 - genetics</subject><subject>Escherichia coli O157 - isolation & purification</subject><subject>Escherichia coli O157:H7</subject><subject>Feces - microbiology</subject><subject>Food Microbiology</subject><subject>Genes, Bacterial</subject><subject>HIV Infections - complications</subject><subject>HIV/AIDS</subject><subject>Human immunodeficiency virus</subject><subject>Humans</subject><subject>Meat</subject><subject>meat and vegetables</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>potential link</subject><subject>Prevalence</subject><subject>South Africa</subject><subject>stool specimens</subject><subject>Vegetables</subject><subject>Water Microbiology</subject><subject>Water Supply</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkV1v0zAUhiMEYmPwF-BccUUy2_lwgsRFtQ1aNDSkMm4tJzlpXRy72O66_VF-D047mG98Pt7z6thPkgAlGY3nfJPRvCpTVnGWMULqjOS8rLL7Z8np_8bzQ1ykJeHsJHnl_YYQmpOyepmc0Jo1dZmT0-TPd4d3UqPpEKTpYWsDmqCkBq3ML2gx7BENXGXQWa3ghpb845yD8lbLgD0Mzo7Qu6hVZgX7WHMfYEQZDm53uMIgW43-kPpgrfZgB-iVdG5tUXXR1wzKjdFrkhhr0qfKfPHzfLa4XMJWBhX38qAMhDXCbJRhbTXCpfLBqS5ACku7C2uYDTGVr5MXg9Qe3zzeZ8nt56sfF_P0-ubL4mJ2nQ45rauUtzmPX9dL3g2FbCgyRru2qftC0lri0PKC9Vw2JZJmaFrKKlI1eUFbWXHCyjw_S94ffbfO_t6hD2JUvkOtpUG784I2VVE0DY_Ct4_CXRtfJrZOjdI9iH8kouDTUbBXGh-e-kRMxMVGTGDFBFZMxMWBuLgXX2ffpijOvzvOD9IKuXLKi9slm4iTJo5WJP8L7w6plg</recordid><startdate>200808</startdate><enddate>200808</enddate><creator>Abong'o, B.O</creator><creator>Momba, M.N.B</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7T7</scope><scope>7U9</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>200808</creationdate><title>Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District - South Africa</title><author>Abong'o, B.O ; Momba, M.N.B</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-f3186-7b37037da7cf4a91e221cb98d4a18aefb742d7a95e09f9b126069341ba6702533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Colony Count, Microbial</topic><topic>Diarrhea - microbiology</topic><topic>Diarrhea - virology</topic><topic>drinking water</topic><topic>E. coli O157:H7</topic><topic>Environmental Microbiology</topic><topic>Escherichia coli Infections - epidemiology</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli O157 - genetics</topic><topic>Escherichia coli O157 - isolation & purification</topic><topic>Escherichia coli O157:H7</topic><topic>Feces - microbiology</topic><topic>Food Microbiology</topic><topic>Genes, Bacterial</topic><topic>HIV Infections - complications</topic><topic>HIV/AIDS</topic><topic>Human immunodeficiency virus</topic><topic>Humans</topic><topic>Meat</topic><topic>meat and vegetables</topic><topic>PCR</topic><topic>Polymerase Chain Reaction - methods</topic><topic>potential link</topic><topic>Prevalence</topic><topic>South Africa</topic><topic>stool specimens</topic><topic>Vegetables</topic><topic>Water Microbiology</topic><topic>Water Supply</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abong'o, B.O</creatorcontrib><creatorcontrib>Momba, M.N.B</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abong'o, B.O</au><au>Momba, M.N.B</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District - South Africa</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2008-08</date><risdate>2008</risdate><volume>105</volume><issue>2</issue><spage>424</spage><epage>431</epage><pages>424-431</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>The current study investigated the prevalence and molecular relatedness between Escherichia coli O157:H7 isolated from water, meat and meat products and vegetables and from stools of confirmed and non-confirmed Human Immune Virus/Acquired Immunodeficiency Syndrome (HIV/AIDS) patients with diarrhoea. Culture-based and polymerase chain reaction techniques were used to identify E. coli O157:H7. Thirty-five per cent of meat products, 25·5% of water, 21·7% of vegetables as well as 56·5% and 43·5% of stools of confirmed and non-confirmed HIV/AIDS patients, respectively, were presumptively positive with E. coli O157. Molecular results indicated that 10·3%, 8·6% and 7·8% of the vegetables, water and meat products examined carried E. coli O157:H7, which had homologous fliCH₇, rfbEO₁₅₇ and eaeA genetic loci to the genes of some E. coli O157:H7 isolated from 12·2% and 8·8% of the stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Water, meat and meat products and vegetables are potential sources of E. coli O157:H7 that are potentially capable of causing diarrhoea in humans especially HIV/AIDS patients. Great care should be exercised to ensure that water and foods consumed by HIV/AIDS patients are safe, as contaminated water and foods can cause secondary infections in these patients.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18298530</pmid><doi>10.1111/j.1365-2672.2008.03756.x</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Colony Count, Microbial Diarrhea - microbiology Diarrhea - virology drinking water E. coli O157:H7 Environmental Microbiology Escherichia coli Infections - epidemiology Escherichia coli Infections - microbiology Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Escherichia coli O157:H7 Feces - microbiology Food Microbiology Genes, Bacterial HIV Infections - complications HIV/AIDS Human immunodeficiency virus Humans Meat meat and vegetables PCR Polymerase Chain Reaction - methods potential link Prevalence South Africa stool specimens Vegetables Water Microbiology Water Supply |
| title | Prevalence and potential link between E. coli O157:H7 isolated from drinking water, meat and vegetables and stools of diarrhoeic confirmed and non-confirmed HIV/AIDS patients in the Amathole District - South Africa |
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