Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis
The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revea...
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| Veröffentlicht in: | Chemistry & biology 2006-06, Vol.13 (6), p.575-585 |
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| creator | Jia, Xin-Ying Tian, Zhen-Hua Shao, Lei Qu, Xu-Dong Zhao, Qun-Fei Tang, Jian Tang, Gong-Li Liu, Wen |
| description | The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from
Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5-chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit. |
| doi_str_mv | 10.1016/j.chembiol.2006.03.008 |
| format | Article |
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Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5-chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit.</description><identifier>ISSN: 1074-5521</identifier><identifier>EISSN: 1879-1301</identifier><identifier>DOI: 10.1016/j.chembiol.2006.03.008</identifier><identifier>PMID: 16793515</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; Aminoglycosides - chemistry ; Aminoglycosides - genetics ; Aminoglycosides - metabolism ; Anti-Bacterial Agents - biosynthesis ; Anti-Bacterial Agents - chemistry ; Carbohydrate Metabolism ; CHEMBIO ; Chlorine - chemistry ; Chromatography, High Pressure Liquid ; Cloning, Molecular ; Conserved Sequence ; Drug Resistance, Bacterial - drug effects ; Gene Expression Regulation, Bacterial ; MICROBIO ; Models, Genetic ; Molecular Sequence Data ; Molecular Structure ; Multigene Family - genetics ; Oxidation-Reduction ; Salicylates - chemistry ; Salicylates - metabolism ; Sequence Alignment ; Streptomyces antibioticus ; Streptomyces antibioticus - genetics ; Streptomyces antibioticus - metabolism</subject><ispartof>Chemistry & biology, 2006-06, Vol.13 (6), p.575-585</ispartof><rights>2006 Elsevier Ltd</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c511t-67dca6a8a72a983c1b10e230329168f9a590c1af860dc50bed43b5cd887739603</citedby><cites>FETCH-LOGICAL-c511t-67dca6a8a72a983c1b10e230329168f9a590c1af860dc50bed43b5cd887739603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1074552106001219$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16793515$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jia, Xin-Ying</creatorcontrib><creatorcontrib>Tian, Zhen-Hua</creatorcontrib><creatorcontrib>Shao, Lei</creatorcontrib><creatorcontrib>Qu, Xu-Dong</creatorcontrib><creatorcontrib>Zhao, Qun-Fei</creatorcontrib><creatorcontrib>Tang, Jian</creatorcontrib><creatorcontrib>Tang, Gong-Li</creatorcontrib><creatorcontrib>Liu, Wen</creatorcontrib><title>Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis</title><title>Chemistry & biology</title><addtitle>Chem Biol</addtitle><description>The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from
Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5-chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit.</description><subject>Amino Acid Sequence</subject><subject>Aminoglycosides - chemistry</subject><subject>Aminoglycosides - genetics</subject><subject>Aminoglycosides - metabolism</subject><subject>Anti-Bacterial Agents - biosynthesis</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Carbohydrate Metabolism</subject><subject>CHEMBIO</subject><subject>Chlorine - chemistry</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>Drug Resistance, Bacterial - drug effects</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>MICROBIO</subject><subject>Models, Genetic</subject><subject>Molecular Sequence Data</subject><subject>Molecular Structure</subject><subject>Multigene Family - genetics</subject><subject>Oxidation-Reduction</subject><subject>Salicylates - chemistry</subject><subject>Salicylates - metabolism</subject><subject>Sequence Alignment</subject><subject>Streptomyces antibioticus</subject><subject>Streptomyces antibioticus - genetics</subject><subject>Streptomyces antibioticus - metabolism</subject><issn>1074-5521</issn><issn>1879-1301</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu1DAQhi0EoqXwCpVP3BJm4tixb5RVKUhFHICz5TgTrVfZeLG9SOXp8WoXceQ01uibf8YfY7cILQKqd7vWb2k_hri0HYBqQbQA-hm7Rj2YBgXg8_qGoW-k7PCKvcp5BwCojXrJrlANRkiU1-zwQCuV4Plm65LzhVL47UqIK48zL1uq_SWmWLYp-LDyE803yzFXkLvMHf8SJ1r4HBP_dggVpJLi6grxu7WEet8p-0OI-WmtaTnk1-zF7JZMby71hv34eP9986l5_PrweXP32HiJWBo1TN4pp93QOaOFxxGBOgGiM6j0bJw04NHNWsHkJYw09WKUftJ6GIRRIG7Y23PuIcWfR8rF7kP2tCxupXjMFo3qe-hlBdUZ9CnmnGi2hxT2Lj1ZBHtybXf2r2t7cm1B2Oq6Dt5eNhzHPU3_xi5yK_D-DFD9569AyWYfaPU0hUS-2CmG_-34A4MTlO4</recordid><startdate>20060601</startdate><enddate>20060601</enddate><creator>Jia, Xin-Ying</creator><creator>Tian, Zhen-Hua</creator><creator>Shao, Lei</creator><creator>Qu, Xu-Dong</creator><creator>Zhao, Qun-Fei</creator><creator>Tang, Jian</creator><creator>Tang, Gong-Li</creator><creator>Liu, Wen</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20060601</creationdate><title>Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis</title><author>Jia, Xin-Ying ; 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| source | MEDLINE; ScienceDirect Journals (5 years ago - present); Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Amino Acid Sequence Aminoglycosides - chemistry Aminoglycosides - genetics Aminoglycosides - metabolism Anti-Bacterial Agents - biosynthesis Anti-Bacterial Agents - chemistry Carbohydrate Metabolism CHEMBIO Chlorine - chemistry Chromatography, High Pressure Liquid Cloning, Molecular Conserved Sequence Drug Resistance, Bacterial - drug effects Gene Expression Regulation, Bacterial MICROBIO Models, Genetic Molecular Sequence Data Molecular Structure Multigene Family - genetics Oxidation-Reduction Salicylates - chemistry Salicylates - metabolism Sequence Alignment Streptomyces antibioticus Streptomyces antibioticus - genetics Streptomyces antibioticus - metabolism |
| title | Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis |
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