Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora
Whey permeate from dairy industry was hydrolyzed enzymatically to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Pseudomonas hydrogenovora. In shaking flask experiments,...
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| Veröffentlicht in: | Bioresource technology 2008-07, Vol.99 (11), p.4854-4863 |
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| creator | Koller, Martin Bona, Rodolfo Chiellini, Emo Fernandes, Elizabeth Grillo Horvat, Predrag Kutschera, Christoph Hesse, Paula Braunegg, Gerhart |
| description | Whey permeate from dairy industry was hydrolyzed enzymatically to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by
Pseudomonas hydrogenovora. In shaking flask experiments, the utilization of whey permeate as a cheap substrate was compared to the utilization of pure glucose and galactose for bacterial growth under balanced conditions as well as for the production of PHB under nitrogen limitation. After determination of the inhibition constant
K
i
for sodium valerate on biomass production (
K
i
=
1.84
g/l), the biosynthesis of PHA co-polyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate was investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions in a bioreactor was performed with hydrolyzed whey permeate to obtain detailed kinetic data (maximum specific growth rate,
μ
max
=
0.29
1/h, maximum polymer concentration, 1.27
g/l PHB), values for molecular mass distribution (weight average molecular weight
M
w
=
353.5
kDa, polydispersity index PDI
=
3.8) and thermo analytical data. The fermentation was repeated with co-feeding of valerate (maximum specific growth rate,
μ
max
=
0.20
1/h, maximum polymer concentration, 1.44
g/l poly-(3HB-
co-21%-3HV), weight average molecular weight
M
w
=
299.2
kDa, polydispersity index PDI
=
4.3). |
| doi_str_mv | 10.1016/j.biortech.2007.09.049 |
| format | Article |
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Pseudomonas hydrogenovora. In shaking flask experiments, the utilization of whey permeate as a cheap substrate was compared to the utilization of pure glucose and galactose for bacterial growth under balanced conditions as well as for the production of PHB under nitrogen limitation. After determination of the inhibition constant
K
i
for sodium valerate on biomass production (
K
i
=
1.84
g/l), the biosynthesis of PHA co-polyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate was investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions in a bioreactor was performed with hydrolyzed whey permeate to obtain detailed kinetic data (maximum specific growth rate,
μ
max
=
0.29
1/h, maximum polymer concentration, 1.27
g/l PHB), values for molecular mass distribution (weight average molecular weight
M
w
=
353.5
kDa, polydispersity index PDI
=
3.8) and thermo analytical data. The fermentation was repeated with co-feeding of valerate (maximum specific growth rate,
μ
max
=
0.20
1/h, maximum polymer concentration, 1.44
g/l poly-(3HB-
co-21%-3HV), weight average molecular weight
M
w
=
299.2
kDa, polydispersity index PDI
=
4.3).</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2007.09.049</identifier><identifier>PMID: 18053709</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>3-Hydroxybutyric Acid - metabolism ; Biological and medical sciences ; Bioreactors ; Fermentation - drug effects ; Fundamental and applied biological sciences. Psychology ; Galactose - pharmacology ; Glucose - pharmacology ; Hydrolysis - drug effects ; Industrial Waste ; Ketoglutaric Acids - metabolism ; Polyesters - isolation & purification ; Polyesters - metabolism ; Polyhydroxyalkanoates ; Polyhydroxyalkanoates - metabolism ; Polymer characterization ; Pseudomonas ; Pseudomonas - cytology ; Pseudomonas - drug effects ; Pseudomonas - growth & development ; Pseudomonas - metabolism ; Pseudomonas hydrogenovora ; Valerates - metabolism ; Valeric acid ; Whey permeate</subject><ispartof>Bioresource technology, 2008-07, Vol.99 (11), p.4854-4863</ispartof><rights>2007 Elsevier Ltd</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-e4244380a0759ec53220f9f11b4d507595aa4a32f8b9b6b3760f43cedf3dc3363</citedby><cites>FETCH-LOGICAL-c451t-e4244380a0759ec53220f9f11b4d507595aa4a32f8b9b6b3760f43cedf3dc3363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biortech.2007.09.049$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20296673$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18053709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koller, Martin</creatorcontrib><creatorcontrib>Bona, Rodolfo</creatorcontrib><creatorcontrib>Chiellini, Emo</creatorcontrib><creatorcontrib>Fernandes, Elizabeth Grillo</creatorcontrib><creatorcontrib>Horvat, Predrag</creatorcontrib><creatorcontrib>Kutschera, Christoph</creatorcontrib><creatorcontrib>Hesse, Paula</creatorcontrib><creatorcontrib>Braunegg, Gerhart</creatorcontrib><title>Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>Whey permeate from dairy industry was hydrolyzed enzymatically to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by
Pseudomonas hydrogenovora. In shaking flask experiments, the utilization of whey permeate as a cheap substrate was compared to the utilization of pure glucose and galactose for bacterial growth under balanced conditions as well as for the production of PHB under nitrogen limitation. After determination of the inhibition constant
K
i
for sodium valerate on biomass production (
K
i
=
1.84
g/l), the biosynthesis of PHA co-polyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate was investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions in a bioreactor was performed with hydrolyzed whey permeate to obtain detailed kinetic data (maximum specific growth rate,
μ
max
=
0.29
1/h, maximum polymer concentration, 1.27
g/l PHB), values for molecular mass distribution (weight average molecular weight
M
w
=
353.5
kDa, polydispersity index PDI
=
3.8) and thermo analytical data. The fermentation was repeated with co-feeding of valerate (maximum specific growth rate,
μ
max
=
0.20
1/h, maximum polymer concentration, 1.44
g/l poly-(3HB-
co-21%-3HV), weight average molecular weight
M
w
=
299.2
kDa, polydispersity index PDI
=
4.3).</description><subject>3-Hydroxybutyric Acid - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bioreactors</subject><subject>Fermentation - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactose - pharmacology</subject><subject>Glucose - pharmacology</subject><subject>Hydrolysis - drug effects</subject><subject>Industrial Waste</subject><subject>Ketoglutaric Acids - metabolism</subject><subject>Polyesters - isolation & purification</subject><subject>Polyesters - metabolism</subject><subject>Polyhydroxyalkanoates</subject><subject>Polyhydroxyalkanoates - metabolism</subject><subject>Polymer characterization</subject><subject>Pseudomonas</subject><subject>Pseudomonas - cytology</subject><subject>Pseudomonas - drug effects</subject><subject>Pseudomonas - growth & development</subject><subject>Pseudomonas - metabolism</subject><subject>Pseudomonas hydrogenovora</subject><subject>Valerates - metabolism</subject><subject>Valeric acid</subject><subject>Whey permeate</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtP3TAQRq2KqtzS_gXIhu6Sjh9x4h0IlbYSUpFa1pbjjLm-TWKwE2j-fXMflGVXI43ON49DyCmFggKVnzdF40Mc0a4LBlAVoAoQ6g1Z0briOVOVPCIrUBLyumTimLxPaQMAnFbsHTmmNZS8ArUi17ehm9dzG8Of2XS_zRDMiNlDDO1kRx-GzMXQZ89rnLNmzm4TTm3ow2BStgvd4xCeQjQfyFtnuoQfD_WE3F1_-XX1Lb_58fX71eVNbkVJxxwFE4LXYKAqFdqSMwZOOUob0ZbbXmmMMJy5ulGNbHglwQlusXW8tZxLfkI-7ecuFz5OmEbd-2Sx68yAYUqaKimoEGoB5R60MaQU0emH6HsTZ01Bbw3qjX4xqLcGNSgNu-DpYcPU9Ni-xg7KFuD8AJhkTeeiGaxP_zgGTElZ8YU723POBG3u48Lc_WRAOUBdC7l75mJP4GLsyWPUyXoclnd9RDvqNvj_XfsXPNCcmg</recordid><startdate>20080701</startdate><enddate>20080701</enddate><creator>Koller, Martin</creator><creator>Bona, Rodolfo</creator><creator>Chiellini, Emo</creator><creator>Fernandes, Elizabeth Grillo</creator><creator>Horvat, Predrag</creator><creator>Kutschera, Christoph</creator><creator>Hesse, Paula</creator><creator>Braunegg, Gerhart</creator><general>Elsevier Ltd</general><general>[New York, NY]: Elsevier Ltd</general><general>Elsevier Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20080701</creationdate><title>Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora</title><author>Koller, Martin ; Bona, Rodolfo ; Chiellini, Emo ; Fernandes, Elizabeth Grillo ; Horvat, Predrag ; Kutschera, Christoph ; Hesse, Paula ; Braunegg, Gerhart</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-e4244380a0759ec53220f9f11b4d507595aa4a32f8b9b6b3760f43cedf3dc3363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>3-Hydroxybutyric Acid - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bioreactors</topic><topic>Fermentation - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galactose - pharmacology</topic><topic>Glucose - pharmacology</topic><topic>Hydrolysis - drug effects</topic><topic>Industrial Waste</topic><topic>Ketoglutaric Acids - metabolism</topic><topic>Polyesters - isolation & purification</topic><topic>Polyesters - metabolism</topic><topic>Polyhydroxyalkanoates</topic><topic>Polyhydroxyalkanoates - metabolism</topic><topic>Polymer characterization</topic><topic>Pseudomonas</topic><topic>Pseudomonas - cytology</topic><topic>Pseudomonas - drug effects</topic><topic>Pseudomonas - growth & development</topic><topic>Pseudomonas - metabolism</topic><topic>Pseudomonas hydrogenovora</topic><topic>Valerates - metabolism</topic><topic>Valeric acid</topic><topic>Whey permeate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koller, Martin</creatorcontrib><creatorcontrib>Bona, Rodolfo</creatorcontrib><creatorcontrib>Chiellini, Emo</creatorcontrib><creatorcontrib>Fernandes, Elizabeth Grillo</creatorcontrib><creatorcontrib>Horvat, Predrag</creatorcontrib><creatorcontrib>Kutschera, Christoph</creatorcontrib><creatorcontrib>Hesse, Paula</creatorcontrib><creatorcontrib>Braunegg, Gerhart</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koller, Martin</au><au>Bona, Rodolfo</au><au>Chiellini, Emo</au><au>Fernandes, Elizabeth Grillo</au><au>Horvat, Predrag</au><au>Kutschera, Christoph</au><au>Hesse, Paula</au><au>Braunegg, Gerhart</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2008-07-01</date><risdate>2008</risdate><volume>99</volume><issue>11</issue><spage>4854</spage><epage>4863</epage><pages>4854-4863</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>Whey permeate from dairy industry was hydrolyzed enzymatically to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by
Pseudomonas hydrogenovora. In shaking flask experiments, the utilization of whey permeate as a cheap substrate was compared to the utilization of pure glucose and galactose for bacterial growth under balanced conditions as well as for the production of PHB under nitrogen limitation. After determination of the inhibition constant
K
i
for sodium valerate on biomass production (
K
i
=
1.84
g/l), the biosynthesis of PHA co-polyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate was investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions in a bioreactor was performed with hydrolyzed whey permeate to obtain detailed kinetic data (maximum specific growth rate,
μ
max
=
0.29
1/h, maximum polymer concentration, 1.27
g/l PHB), values for molecular mass distribution (weight average molecular weight
M
w
=
353.5
kDa, polydispersity index PDI
=
3.8) and thermo analytical data. The fermentation was repeated with co-feeding of valerate (maximum specific growth rate,
μ
max
=
0.20
1/h, maximum polymer concentration, 1.44
g/l poly-(3HB-
co-21%-3HV), weight average molecular weight
M
w
=
299.2
kDa, polydispersity index PDI
=
4.3).</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>18053709</pmid><doi>10.1016/j.biortech.2007.09.049</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Bioresource technology, 2008-07, Vol.99 (11), p.4854-4863 |
| issn | 0960-8524 1873-2976 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 3-Hydroxybutyric Acid - metabolism Biological and medical sciences Bioreactors Fermentation - drug effects Fundamental and applied biological sciences. Psychology Galactose - pharmacology Glucose - pharmacology Hydrolysis - drug effects Industrial Waste Ketoglutaric Acids - metabolism Polyesters - isolation & purification Polyesters - metabolism Polyhydroxyalkanoates Polyhydroxyalkanoates - metabolism Polymer characterization Pseudomonas Pseudomonas - cytology Pseudomonas - drug effects Pseudomonas - growth & development Pseudomonas - metabolism Pseudomonas hydrogenovora Valerates - metabolism Valeric acid Whey permeate |
| title | Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora |
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