Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon Schrank): Analysis of genetic diversity and differentiation
Genetic diversity was investigated in 73 accessions of emmer wheat (Triticum dicoccon Schrank) from 11 geographical regions using a set of 29 simple-sequence repeat (SSR or microsatellite) markers, representing at least two markers for each chromosome. The SSR primers amplified a total of 357 differ...
Gespeichert in:
| Veröffentlicht in: | Genetic resources and crop evolution 2007-05, Vol.54 (3), p.543-554 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 554 |
|---|---|
| container_issue | 3 |
| container_start_page | 543 |
| container_title | Genetic resources and crop evolution |
| container_volume | 54 |
| creator | Teklu, Yifru Hammer, K. Röder, M. S. |
| description | Genetic diversity was investigated in 73 accessions of emmer wheat (Triticum dicoccon Schrank) from 11 geographical regions using a set of 29 simple-sequence repeat (SSR or microsatellite) markers, representing at least two markers for each chromosome. The SSR primers amplified a total of 357 different alleles with an average of 12.31 alleles per locus. The number of fragments detected by each primer ranged between 6 (Xgwm1066) and 21 (Xgwm268). Null alleles were detected in nine of the 29 primers used. A high level of gene diversity index was observed. Across the 29 primers, gene diversity ranged from 0.60 (Xgwm46) to 0.94 (Xgwm655), with a mean of 0.82. There was a highly significant correlation (r=0.882; p |
| doi_str_mv | 10.1007/s10722-006-0011-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_19634863</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2259832314</sourcerecordid><originalsourceid>FETCH-LOGICAL-c234t-f1a7b74fc829245f0636e05175f1c4ccbcf45697bec21457e1ceda5954de81f63</originalsourceid><addsrcrecordid>eNpdkU2LFDEQhoO44Lj6A7wFBHEPral8drwti1-w4GHXc8hkKk52u5M26XGZn-C_3izjyUNRVPFQ8NZDyBtgH4Ax87EBM5wPjOleAIN5RjagjBgUMPucbJjlarCjli_Iy9buGGPW6HFD_t6keZmQNvx9wByQVlzQr43Ovt5jpUuZjnOpyz61maZMcZ779mHfGfr-tqY1hcNMdymUEEqmN2Fffb6_-EQvs5-OLTVaIv2FGTvYsT9YW1qP1Oddn2LEinlNfk0lvyJn0U8NX__r5-Tnl8-3V9-G6x9fv19dXg-BC7kOEbzZGhnDyC2XKjItNDIFRkUIMoRtiFJpa7YYOEhlEALuvLJK7nCEqMU5eXe6u9TSM7fVzakFnCafsRyaA6uFHLXo4Nv_wLtyqD1Wc5wrOwouQHYKTlSopbWK0S019ecdHTD3pMad1Liuxj2pcUY8Akd7hAc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2259832314</pqid></control><display><type>article</type><title>Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon Schrank): Analysis of genetic diversity and differentiation</title><source>SpringerLink Journals - AutoHoldings</source><creator>Teklu, Yifru ; Hammer, K. ; Röder, M. S.</creator><creatorcontrib>Teklu, Yifru ; Hammer, K. ; Röder, M. S.</creatorcontrib><description>Genetic diversity was investigated in 73 accessions of emmer wheat (Triticum dicoccon Schrank) from 11 geographical regions using a set of 29 simple-sequence repeat (SSR or microsatellite) markers, representing at least two markers for each chromosome. The SSR primers amplified a total of 357 different alleles with an average of 12.31 alleles per locus. The number of fragments detected by each primer ranged between 6 (Xgwm1066) and 21 (Xgwm268). Null alleles were detected in nine of the 29 primers used. A high level of gene diversity index was observed. Across the 29 primers, gene diversity ranged from 0.60 (Xgwm46) to 0.94 (Xgwm655), with a mean of 0.82. There was a highly significant correlation (r=0.882; p<0.01) between gene diversity index and the number of loci, showing the number of loci per se is a strong indicator of diversity. Analysis of genetic diversity within and among eleven geographical regions revealed most of the genetic diversity of the total sample resided within regions. The coefficient of gene differentiation (Gst = 0.27) showed that the genetic variation within and among the 11 geographical regions was 73 and 27%, respectively. High value of mean number of alleles per locus was found in Iran (4.86) followed by Morocco (4.10) and Armenia (4.03). On the contrary, lower mean number of alleles per locus was detected in Yemen (2.83). The average gene diversity index across regions ranged from 0.52 (Slovakia) to 0.67 (Morocco) with an average of 0.60. Multivariate techniques of principal component analysis and clustering were employed to examine genetic relationship among the 73 emmer wheat accessions vis-à-vis geographical regions of collections. The genetic distance coefficients for all possible 55 pairs of regional comparisons ranged from 0.63 (between Iran and Armenia, Georgia and Azerbaijan, Georgia and Slovakia) to 0.97 (between Morocco and Yemen, Spain and Georgia, and Turkey and Iran) with a mean of 0.82. From the PCA results, a two dimensional plot of PC1 versus PC2 was constructed. The scatter plot of the first two principal components which explained altogether 27% of the total variation depicted the presence of a clear pattern of geographical differentiation except in few cases like accessions from Caucasian region. Similar pattern of genetic relationships among accessions was observed in cluster analysis. The study provided genetic information of emmer wheat in relation to geographical regions of origin. The information could be utilized in crop improvement, germplasm conservation programs, and in further investigation.</description><identifier>ISSN: 0925-9864</identifier><identifier>EISSN: 1573-5109</identifier><identifier>DOI: 10.1007/s10722-006-0011-7</identifier><language>eng</language><publisher>Dordrecht: Springer Nature B.V</publisher><subject>Alleles ; Chromosomes ; Cluster analysis ; Clustering ; Coefficient of variation ; Crop improvement ; Differentiation ; Gene polymorphism ; Genetic analysis ; Genetic distance ; Genetic diversity ; Genetic relationship ; Germplasm ; Information processing ; Loci ; Markers ; Microsatellites ; Polymorphism ; Primers ; Principal components analysis ; Simple sequence repeats ; Triticum dicoccum ; Wheat</subject><ispartof>Genetic resources and crop evolution, 2007-05, Vol.54 (3), p.543-554</ispartof><rights>Genetic Resources and Crop Evolution is a copyright of Springer, (2006). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c234t-f1a7b74fc829245f0636e05175f1c4ccbcf45697bec21457e1ceda5954de81f63</citedby><cites>FETCH-LOGICAL-c234t-f1a7b74fc829245f0636e05175f1c4ccbcf45697bec21457e1ceda5954de81f63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Teklu, Yifru</creatorcontrib><creatorcontrib>Hammer, K.</creatorcontrib><creatorcontrib>Röder, M. S.</creatorcontrib><title>Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon Schrank): Analysis of genetic diversity and differentiation</title><title>Genetic resources and crop evolution</title><description>Genetic diversity was investigated in 73 accessions of emmer wheat (Triticum dicoccon Schrank) from 11 geographical regions using a set of 29 simple-sequence repeat (SSR or microsatellite) markers, representing at least two markers for each chromosome. The SSR primers amplified a total of 357 different alleles with an average of 12.31 alleles per locus. The number of fragments detected by each primer ranged between 6 (Xgwm1066) and 21 (Xgwm268). Null alleles were detected in nine of the 29 primers used. A high level of gene diversity index was observed. Across the 29 primers, gene diversity ranged from 0.60 (Xgwm46) to 0.94 (Xgwm655), with a mean of 0.82. There was a highly significant correlation (r=0.882; p<0.01) between gene diversity index and the number of loci, showing the number of loci per se is a strong indicator of diversity. Analysis of genetic diversity within and among eleven geographical regions revealed most of the genetic diversity of the total sample resided within regions. The coefficient of gene differentiation (Gst = 0.27) showed that the genetic variation within and among the 11 geographical regions was 73 and 27%, respectively. High value of mean number of alleles per locus was found in Iran (4.86) followed by Morocco (4.10) and Armenia (4.03). On the contrary, lower mean number of alleles per locus was detected in Yemen (2.83). The average gene diversity index across regions ranged from 0.52 (Slovakia) to 0.67 (Morocco) with an average of 0.60. Multivariate techniques of principal component analysis and clustering were employed to examine genetic relationship among the 73 emmer wheat accessions vis-à-vis geographical regions of collections. The genetic distance coefficients for all possible 55 pairs of regional comparisons ranged from 0.63 (between Iran and Armenia, Georgia and Azerbaijan, Georgia and Slovakia) to 0.97 (between Morocco and Yemen, Spain and Georgia, and Turkey and Iran) with a mean of 0.82. From the PCA results, a two dimensional plot of PC1 versus PC2 was constructed. The scatter plot of the first two principal components which explained altogether 27% of the total variation depicted the presence of a clear pattern of geographical differentiation except in few cases like accessions from Caucasian region. Similar pattern of genetic relationships among accessions was observed in cluster analysis. The study provided genetic information of emmer wheat in relation to geographical regions of origin. The information could be utilized in crop improvement, germplasm conservation programs, and in further investigation.</description><subject>Alleles</subject><subject>Chromosomes</subject><subject>Cluster analysis</subject><subject>Clustering</subject><subject>Coefficient of variation</subject><subject>Crop improvement</subject><subject>Differentiation</subject><subject>Gene polymorphism</subject><subject>Genetic analysis</subject><subject>Genetic distance</subject><subject>Genetic diversity</subject><subject>Genetic relationship</subject><subject>Germplasm</subject><subject>Information processing</subject><subject>Loci</subject><subject>Markers</subject><subject>Microsatellites</subject><subject>Polymorphism</subject><subject>Primers</subject><subject>Principal components analysis</subject><subject>Simple sequence repeats</subject><subject>Triticum dicoccum</subject><subject>Wheat</subject><issn>0925-9864</issn><issn>1573-5109</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkU2LFDEQhoO44Lj6A7wFBHEPral8drwti1-w4GHXc8hkKk52u5M26XGZn-C_3izjyUNRVPFQ8NZDyBtgH4Ax87EBM5wPjOleAIN5RjagjBgUMPucbJjlarCjli_Iy9buGGPW6HFD_t6keZmQNvx9wByQVlzQr43Ovt5jpUuZjnOpyz61maZMcZ779mHfGfr-tqY1hcNMdymUEEqmN2Fffb6_-EQvs5-OLTVaIv2FGTvYsT9YW1qP1Oddn2LEinlNfk0lvyJn0U8NX__r5-Tnl8-3V9-G6x9fv19dXg-BC7kOEbzZGhnDyC2XKjItNDIFRkUIMoRtiFJpa7YYOEhlEALuvLJK7nCEqMU5eXe6u9TSM7fVzakFnCafsRyaA6uFHLXo4Nv_wLtyqD1Wc5wrOwouQHYKTlSopbWK0S019ecdHTD3pMad1Liuxj2pcUY8Akd7hAc</recordid><startdate>20070501</startdate><enddate>20070501</enddate><creator>Teklu, Yifru</creator><creator>Hammer, K.</creator><creator>Röder, M. S.</creator><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20070501</creationdate><title>Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon Schrank): Analysis of genetic diversity and differentiation</title><author>Teklu, Yifru ; Hammer, K. ; Röder, M. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c234t-f1a7b74fc829245f0636e05175f1c4ccbcf45697bec21457e1ceda5954de81f63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Alleles</topic><topic>Chromosomes</topic><topic>Cluster analysis</topic><topic>Clustering</topic><topic>Coefficient of variation</topic><topic>Crop improvement</topic><topic>Differentiation</topic><topic>Gene polymorphism</topic><topic>Genetic analysis</topic><topic>Genetic distance</topic><topic>Genetic diversity</topic><topic>Genetic relationship</topic><topic>Germplasm</topic><topic>Information processing</topic><topic>Loci</topic><topic>Markers</topic><topic>Microsatellites</topic><topic>Polymorphism</topic><topic>Primers</topic><topic>Principal components analysis</topic><topic>Simple sequence repeats</topic><topic>Triticum dicoccum</topic><topic>Wheat</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teklu, Yifru</creatorcontrib><creatorcontrib>Hammer, K.</creatorcontrib><creatorcontrib>Röder, M. S.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Genetic resources and crop evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teklu, Yifru</au><au>Hammer, K.</au><au>Röder, M. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon Schrank): Analysis of genetic diversity and differentiation</atitle><jtitle>Genetic resources and crop evolution</jtitle><date>2007-05-01</date><risdate>2007</risdate><volume>54</volume><issue>3</issue><spage>543</spage><epage>554</epage><pages>543-554</pages><issn>0925-9864</issn><eissn>1573-5109</eissn><abstract>Genetic diversity was investigated in 73 accessions of emmer wheat (Triticum dicoccon Schrank) from 11 geographical regions using a set of 29 simple-sequence repeat (SSR or microsatellite) markers, representing at least two markers for each chromosome. The SSR primers amplified a total of 357 different alleles with an average of 12.31 alleles per locus. The number of fragments detected by each primer ranged between 6 (Xgwm1066) and 21 (Xgwm268). Null alleles were detected in nine of the 29 primers used. A high level of gene diversity index was observed. Across the 29 primers, gene diversity ranged from 0.60 (Xgwm46) to 0.94 (Xgwm655), with a mean of 0.82. There was a highly significant correlation (r=0.882; p<0.01) between gene diversity index and the number of loci, showing the number of loci per se is a strong indicator of diversity. Analysis of genetic diversity within and among eleven geographical regions revealed most of the genetic diversity of the total sample resided within regions. The coefficient of gene differentiation (Gst = 0.27) showed that the genetic variation within and among the 11 geographical regions was 73 and 27%, respectively. High value of mean number of alleles per locus was found in Iran (4.86) followed by Morocco (4.10) and Armenia (4.03). On the contrary, lower mean number of alleles per locus was detected in Yemen (2.83). The average gene diversity index across regions ranged from 0.52 (Slovakia) to 0.67 (Morocco) with an average of 0.60. Multivariate techniques of principal component analysis and clustering were employed to examine genetic relationship among the 73 emmer wheat accessions vis-à-vis geographical regions of collections. The genetic distance coefficients for all possible 55 pairs of regional comparisons ranged from 0.63 (between Iran and Armenia, Georgia and Azerbaijan, Georgia and Slovakia) to 0.97 (between Morocco and Yemen, Spain and Georgia, and Turkey and Iran) with a mean of 0.82. From the PCA results, a two dimensional plot of PC1 versus PC2 was constructed. The scatter plot of the first two principal components which explained altogether 27% of the total variation depicted the presence of a clear pattern of geographical differentiation except in few cases like accessions from Caucasian region. Similar pattern of genetic relationships among accessions was observed in cluster analysis. The study provided genetic information of emmer wheat in relation to geographical regions of origin. The information could be utilized in crop improvement, germplasm conservation programs, and in further investigation.</abstract><cop>Dordrecht</cop><pub>Springer Nature B.V</pub><doi>10.1007/s10722-006-0011-7</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0925-9864 |
| ispartof | Genetic resources and crop evolution, 2007-05, Vol.54 (3), p.543-554 |
| issn | 0925-9864 1573-5109 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_19634863 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Alleles Chromosomes Cluster analysis Clustering Coefficient of variation Crop improvement Differentiation Gene polymorphism Genetic analysis Genetic distance Genetic diversity Genetic relationship Germplasm Information processing Loci Markers Microsatellites Polymorphism Primers Principal components analysis Simple sequence repeats Triticum dicoccum Wheat |
| title | Simple sequence repeats marker polymorphism in emmer wheat (Triticum dicoccon Schrank): Analysis of genetic diversity and differentiation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T14%3A15%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Simple%20sequence%20repeats%20marker%20polymorphism%20in%20emmer%20wheat%20(Triticum%20dicoccon%20Schrank):%20Analysis%20of%20genetic%20diversity%20and%20differentiation&rft.jtitle=Genetic%20resources%20and%20crop%20evolution&rft.au=Teklu,%20Yifru&rft.date=2007-05-01&rft.volume=54&rft.issue=3&rft.spage=543&rft.epage=554&rft.pages=543-554&rft.issn=0925-9864&rft.eissn=1573-5109&rft_id=info:doi/10.1007/s10722-006-0011-7&rft_dat=%3Cproquest_cross%3E2259832314%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2259832314&rft_id=info:pmid/&rfr_iscdi=true |