Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids
The aim of the present study was to investigate how maternal diet can influence the adipose tissue of goat kids. Omental adipose tissue proteomes of goat-kids from mothers fed with diet enriched with stearic acid (ST-kids), fish oil (FO-kids) and standard diets (CTRL) were determined by quantitative...
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| Veröffentlicht in: | Research in veterinary science 2019-08, Vol.125, p.451-458 |
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| creator | Restelli, Laura Marques, Andreia T. Savoini, Giovanni Invernizzi, Guido Carisetti, Michela Lecchi, Cristina Bendixen, Emoke Ceciliani, Fabrizio |
| description | The aim of the present study was to investigate how maternal diet can influence the adipose tissue of goat kids. Omental adipose tissue proteomes of goat-kids from mothers fed with diet enriched with stearic acid (ST-kids), fish oil (FO-kids) and standard diets (CTRL) were determined by quantitative iTRAQ 2D-LC-MS/MS analysis. Twenty proteins were found to be differentially expressed in suckling kids' omental adipose tissue. Stearic acid induces changes in a higher number of proteins when compared to fish oil. Eleven proteins, namely AARS, ECl1, PMSC2, CP, HSPA8, GPD1, RPL7, OGDH, RPL24, FGA and RPL5 were decreased in ST-kids only. Four proteins, namely DLST, EEF1G, BCAP31 and RALA were decreased in FO-kids only, and one, NUCKS1, was increased. Four proteins, namely PMSC1, PPIB, TUB5×2 and EIF5A1, were be less abundant in both ST- and FO- kids. Most of the protein whose abundance was decreased in ST kids (10 out of 15) are involved in protein metabolism and catabolism pathways. Qualitative gene expression analysis confirmed that all the proteins identified by mass spectrometry, with the exception of FGA, were produced by adipose tissue. Quantitative gene expression analysis demonstrated that two proteins, namely CP, a minor acute phase protein, and ECl1, involved in fatty acid beta oxidation, were downregulated at mRNA level as well. ECl1 gene expression was downregulated in ST-kids AT as compared to Ctrl-kids and CP was downregulated in both ST- and FO-kids. The present results demonstrate that it is possible to influence adipose goat-kid proteome by modifying the maternal diet.
•Quantitative proteomics was carried out on goat-kid omental adipose tissue after supplementing lactating mothers with different fatty acids.•Different diets modified the omental adipose tissue proteome.•Twenty proteins were found to be differentially expressed, of which only NUCKS1, was overexpressed.•The downregulation of ECl1 and Ceruloplasmin was also confirmed at gene expression level.•The results demonstrated that supplementing other diet with different PUFA may influence omental adipose tissue proteome. |
| doi_str_mv | 10.1016/j.rvsc.2017.10.009 |
| format | Article |
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•Quantitative proteomics was carried out on goat-kid omental adipose tissue after supplementing lactating mothers with different fatty acids.•Different diets modified the omental adipose tissue proteome.•Twenty proteins were found to be differentially expressed, of which only NUCKS1, was overexpressed.•The downregulation of ECl1 and Ceruloplasmin was also confirmed at gene expression level.•The results demonstrated that supplementing other diet with different PUFA may influence omental adipose tissue proteome.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2017.10.009</identifier><identifier>PMID: 29128114</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acute phase proteins ; Adipose tissue ; Animal behavior ; Body fat ; Catabolism ; Diet ; Fatty acids ; Fish oil ; Fish oils ; Gene expression ; Glycerol-3-phosphate dehydrogenase ; Goat ; Goats ; Investigations ; Mass spectrometry ; Mass spectroscopy ; Oils & fats ; Oxidation ; Peripartum ; Protein metabolism ; Protein turnover ; Proteins ; Proteomes ; Proteomics ; Qualitative analysis ; Stearic acid ; Suckling behavior ; Tissue analysis ; Two dimensional analysis ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2019-08, Vol.125, p.451-458</ispartof><rights>2017 Elsevier Ltd</rights><rights>Copyright © 2017 Elsevier Ltd. All rights reserved.</rights><rights>2017. Elsevier Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-8d01a47ffd7f38d4fcb006814007cc614ac3acda356479d070b3c353ef3915923</citedby><cites>FETCH-LOGICAL-c428t-8d01a47ffd7f38d4fcb006814007cc614ac3acda356479d070b3c353ef3915923</cites><orcidid>0000-0002-8377-9376 ; 0000-0002-6318-448X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2017.10.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29128114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Restelli, Laura</creatorcontrib><creatorcontrib>Marques, Andreia T.</creatorcontrib><creatorcontrib>Savoini, Giovanni</creatorcontrib><creatorcontrib>Invernizzi, Guido</creatorcontrib><creatorcontrib>Carisetti, Michela</creatorcontrib><creatorcontrib>Lecchi, Cristina</creatorcontrib><creatorcontrib>Bendixen, Emoke</creatorcontrib><creatorcontrib>Ceciliani, Fabrizio</creatorcontrib><title>Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>The aim of the present study was to investigate how maternal diet can influence the adipose tissue of goat kids. Omental adipose tissue proteomes of goat-kids from mothers fed with diet enriched with stearic acid (ST-kids), fish oil (FO-kids) and standard diets (CTRL) were determined by quantitative iTRAQ 2D-LC-MS/MS analysis. Twenty proteins were found to be differentially expressed in suckling kids' omental adipose tissue. Stearic acid induces changes in a higher number of proteins when compared to fish oil. Eleven proteins, namely AARS, ECl1, PMSC2, CP, HSPA8, GPD1, RPL7, OGDH, RPL24, FGA and RPL5 were decreased in ST-kids only. Four proteins, namely DLST, EEF1G, BCAP31 and RALA were decreased in FO-kids only, and one, NUCKS1, was increased. Four proteins, namely PMSC1, PPIB, TUB5×2 and EIF5A1, were be less abundant in both ST- and FO- kids. Most of the protein whose abundance was decreased in ST kids (10 out of 15) are involved in protein metabolism and catabolism pathways. Qualitative gene expression analysis confirmed that all the proteins identified by mass spectrometry, with the exception of FGA, were produced by adipose tissue. Quantitative gene expression analysis demonstrated that two proteins, namely CP, a minor acute phase protein, and ECl1, involved in fatty acid beta oxidation, were downregulated at mRNA level as well. ECl1 gene expression was downregulated in ST-kids AT as compared to Ctrl-kids and CP was downregulated in both ST- and FO-kids. The present results demonstrate that it is possible to influence adipose goat-kid proteome by modifying the maternal diet.
•Quantitative proteomics was carried out on goat-kid omental adipose tissue after supplementing lactating mothers with different fatty acids.•Different diets modified the omental adipose tissue proteome.•Twenty proteins were found to be differentially expressed, of which only NUCKS1, was overexpressed.•The downregulation of ECl1 and Ceruloplasmin was also confirmed at gene expression level.•The results demonstrated that supplementing other diet with different PUFA may influence omental adipose tissue proteome.</description><subject>Acute phase proteins</subject><subject>Adipose tissue</subject><subject>Animal behavior</subject><subject>Body fat</subject><subject>Catabolism</subject><subject>Diet</subject><subject>Fatty acids</subject><subject>Fish oil</subject><subject>Fish oils</subject><subject>Gene expression</subject><subject>Glycerol-3-phosphate dehydrogenase</subject><subject>Goat</subject><subject>Goats</subject><subject>Investigations</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Oils & fats</subject><subject>Oxidation</subject><subject>Peripartum</subject><subject>Protein metabolism</subject><subject>Protein turnover</subject><subject>Proteins</subject><subject>Proteomes</subject><subject>Proteomics</subject><subject>Qualitative analysis</subject><subject>Stearic acid</subject><subject>Suckling behavior</subject><subject>Tissue analysis</subject><subject>Two dimensional analysis</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kUtv1TAQhS0EopfCH2CBLLFhk9vxI3EisUEVL6kSi5a15WuPK98mcbCdiv57HN3SBQtWozn-5mg8h5C3DPYMWHdx3Kf7bPccmKrCHmB4RnasFbzhXceekx2AkE3L-_6MvMr5CACSMfWSnPGB8Z4xuSO_r01ZkynoaEx0nfNT602heV2WESecN2GqcprNSF3AkmmY_bjibJHGDai6cWGJGWkJOa9IlxQL1jcafTWyd2OYb-ltNKW5Cy6_Ji-8GTO-eazn5OeXzzeX35qrH1-_X366aqzkfWl6B8xI5b1TXvROensA6HomAZS1HZPGCmOdEW0n1eBAwUFY0Qr0YmDtwMU5-XDyrev8WjEXPYVscRzNjHHNmg2dkB1UuKLv_0GPcd1-nDXnSg28V6qvFD9RNsWcE3q9pDCZ9KAZ6C0XfdRbLnrLZdNqLnXo3aP1epjQPY38DaICH08A1lvcB0w627Bd14WEtmgXw__8_wCraaCS</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Restelli, Laura</creator><creator>Marques, Andreia T.</creator><creator>Savoini, Giovanni</creator><creator>Invernizzi, Guido</creator><creator>Carisetti, Michela</creator><creator>Lecchi, Cristina</creator><creator>Bendixen, Emoke</creator><creator>Ceciliani, Fabrizio</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8377-9376</orcidid><orcidid>https://orcid.org/0000-0002-6318-448X</orcidid></search><sort><creationdate>201908</creationdate><title>Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids</title><author>Restelli, Laura ; Marques, Andreia T. ; Savoini, Giovanni ; Invernizzi, Guido ; Carisetti, Michela ; Lecchi, Cristina ; Bendixen, Emoke ; Ceciliani, Fabrizio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-8d01a47ffd7f38d4fcb006814007cc614ac3acda356479d070b3c353ef3915923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acute phase proteins</topic><topic>Adipose tissue</topic><topic>Animal behavior</topic><topic>Body fat</topic><topic>Catabolism</topic><topic>Diet</topic><topic>Fatty acids</topic><topic>Fish oil</topic><topic>Fish oils</topic><topic>Gene expression</topic><topic>Glycerol-3-phosphate dehydrogenase</topic><topic>Goat</topic><topic>Goats</topic><topic>Investigations</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Oils & fats</topic><topic>Oxidation</topic><topic>Peripartum</topic><topic>Protein metabolism</topic><topic>Protein turnover</topic><topic>Proteins</topic><topic>Proteomes</topic><topic>Proteomics</topic><topic>Qualitative analysis</topic><topic>Stearic acid</topic><topic>Suckling behavior</topic><topic>Tissue analysis</topic><topic>Two dimensional analysis</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Restelli, Laura</creatorcontrib><creatorcontrib>Marques, Andreia T.</creatorcontrib><creatorcontrib>Savoini, Giovanni</creatorcontrib><creatorcontrib>Invernizzi, Guido</creatorcontrib><creatorcontrib>Carisetti, Michela</creatorcontrib><creatorcontrib>Lecchi, Cristina</creatorcontrib><creatorcontrib>Bendixen, Emoke</creatorcontrib><creatorcontrib>Ceciliani, Fabrizio</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Restelli, Laura</au><au>Marques, Andreia T.</au><au>Savoini, Giovanni</au><au>Invernizzi, Guido</au><au>Carisetti, Michela</au><au>Lecchi, Cristina</au><au>Bendixen, Emoke</au><au>Ceciliani, Fabrizio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2019-08</date><risdate>2019</risdate><volume>125</volume><spage>451</spage><epage>458</epage><pages>451-458</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>The aim of the present study was to investigate how maternal diet can influence the adipose tissue of goat kids. Omental adipose tissue proteomes of goat-kids from mothers fed with diet enriched with stearic acid (ST-kids), fish oil (FO-kids) and standard diets (CTRL) were determined by quantitative iTRAQ 2D-LC-MS/MS analysis. Twenty proteins were found to be differentially expressed in suckling kids' omental adipose tissue. Stearic acid induces changes in a higher number of proteins when compared to fish oil. Eleven proteins, namely AARS, ECl1, PMSC2, CP, HSPA8, GPD1, RPL7, OGDH, RPL24, FGA and RPL5 were decreased in ST-kids only. Four proteins, namely DLST, EEF1G, BCAP31 and RALA were decreased in FO-kids only, and one, NUCKS1, was increased. Four proteins, namely PMSC1, PPIB, TUB5×2 and EIF5A1, were be less abundant in both ST- and FO- kids. Most of the protein whose abundance was decreased in ST kids (10 out of 15) are involved in protein metabolism and catabolism pathways. Qualitative gene expression analysis confirmed that all the proteins identified by mass spectrometry, with the exception of FGA, were produced by adipose tissue. Quantitative gene expression analysis demonstrated that two proteins, namely CP, a minor acute phase protein, and ECl1, involved in fatty acid beta oxidation, were downregulated at mRNA level as well. ECl1 gene expression was downregulated in ST-kids AT as compared to Ctrl-kids and CP was downregulated in both ST- and FO-kids. The present results demonstrate that it is possible to influence adipose goat-kid proteome by modifying the maternal diet.
•Quantitative proteomics was carried out on goat-kid omental adipose tissue after supplementing lactating mothers with different fatty acids.•Different diets modified the omental adipose tissue proteome.•Twenty proteins were found to be differentially expressed, of which only NUCKS1, was overexpressed.•The downregulation of ECl1 and Ceruloplasmin was also confirmed at gene expression level.•The results demonstrated that supplementing other diet with different PUFA may influence omental adipose tissue proteome.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29128114</pmid><doi>10.1016/j.rvsc.2017.10.009</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-8377-9376</orcidid><orcidid>https://orcid.org/0000-0002-6318-448X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Acute phase proteins Adipose tissue Animal behavior Body fat Catabolism Diet Fatty acids Fish oil Fish oils Gene expression Glycerol-3-phosphate dehydrogenase Goat Goats Investigations Mass spectrometry Mass spectroscopy Oils & fats Oxidation Peripartum Protein metabolism Protein turnover Proteins Proteomes Proteomics Qualitative analysis Stearic acid Suckling behavior Tissue analysis Two dimensional analysis Veterinary medicine |
| title | Saturated or unsaturated fat supplemented maternal diets influence omental adipose tissue proteome of suckling goat-kids |
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