Patterns of storage protein and triacylglycerol accumulation during loblolly pine somatic embryo maturation
Conifer somatic embryo germination and early seedling growth are fundamentally different than in their zygotic counterparts in that the living maternal megagametophyte tissue surrounding the embryo is absent. The megagametophyte contains the majority of the seed storage reserves in loblolly pine and...
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| Veröffentlicht in: | Plant cell, tissue and organ culture tissue and organ culture, 2007-02, Vol.88 (2), p.217-223 |
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| container_title | Plant cell, tissue and organ culture |
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| creator | Brownfield, Disa L Todd, Christopher D Stone, Sandra L Deyholos, Michael K Gifford, David J |
| description | Conifer somatic embryo germination and early seedling growth are fundamentally different than in their zygotic counterparts in that the living maternal megagametophyte tissue surrounding the embryo is absent. The megagametophyte contains the majority of the seed storage reserves in loblolly pine and the lack of the megagametophyte tissue poses a significant challenge to somatic embryo germination and growth. We investigated the differences in seed storage reserves between loblolly pine mature zygotic embryos and somatic embryos that were capable of germination and early seedling growth. Somatic embryos utilized in this study contained significantly lower levels of triacylglycerol and higher levels of storage proteins relative to zygotic embryos. A shift in the ratio of soluble to insoluble protein present was also observed. Mature zygotic embryos had roughly a 3:2 ratio of soluble to insoluble protein whereas the somatic embryos contained over 5-fold more soluble protein compared to insoluble protein. This indicates that the somatic embryos are not only producing more protein overall, but that this protein is biased more heavily towards soluble protein, indicating possible differences in metabolic activity at the time of desiccation. |
| doi_str_mv | 10.1007/s11240-006-9193-0 |
| format | Article |
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This indicates that the somatic embryos are not only producing more protein overall, but that this protein is biased more heavily towards soluble protein, indicating possible differences in metabolic activity at the time of desiccation.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>chemical constituents of plants</subject><subject>developmental stages</subject><subject>Eukaryotic cell cultures</subject><subject>forest trees</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Miscellaneous</topic><topic>Pinus taeda</topic><topic>Plant cells and fungal cells</topic><topic>plant development</topic><topic>protein metabolism</topic><topic>quantitative analysis</topic><topic>seed germination</topic><topic>seedling growth</topic><topic>seeds</topic><topic>somatic embryos</topic><topic>storage proteins</topic><topic>triacylglycerols</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brownfield, Disa L</creatorcontrib><creatorcontrib>Todd, Christopher D</creatorcontrib><creatorcontrib>Stone, Sandra L</creatorcontrib><creatorcontrib>Deyholos, Michael K</creatorcontrib><creatorcontrib>Gifford, David J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant cell, tissue and organ culture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brownfield, Disa L</au><au>Todd, Christopher D</au><au>Stone, Sandra L</au><au>Deyholos, Michael K</au><au>Gifford, David J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Patterns of storage protein and triacylglycerol accumulation during loblolly pine somatic embryo maturation</atitle><jtitle>Plant cell, tissue and organ culture</jtitle><date>2007-02-01</date><risdate>2007</risdate><volume>88</volume><issue>2</issue><spage>217</spage><epage>223</epage><pages>217-223</pages><issn>0167-6857</issn><eissn>1573-5044</eissn><coden>PTCEDJ</coden><abstract>Conifer somatic embryo germination and early seedling growth are fundamentally different than in their zygotic counterparts in that the living maternal megagametophyte tissue surrounding the embryo is absent. The megagametophyte contains the majority of the seed storage reserves in loblolly pine and the lack of the megagametophyte tissue poses a significant challenge to somatic embryo germination and growth. We investigated the differences in seed storage reserves between loblolly pine mature zygotic embryos and somatic embryos that were capable of germination and early seedling growth. Somatic embryos utilized in this study contained significantly lower levels of triacylglycerol and higher levels of storage proteins relative to zygotic embryos. A shift in the ratio of soluble to insoluble protein present was also observed. Mature zygotic embryos had roughly a 3:2 ratio of soluble to insoluble protein whereas the somatic embryos contained over 5-fold more soluble protein compared to insoluble protein. 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| ispartof | Plant cell, tissue and organ culture, 2007-02, Vol.88 (2), p.217-223 |
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| subjects | Biological and medical sciences Biotechnology chemical constituents of plants developmental stages Eukaryotic cell cultures forest trees Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Miscellaneous Pinus taeda Plant cells and fungal cells plant development protein metabolism quantitative analysis seed germination seedling growth seeds somatic embryos storage proteins triacylglycerols |
| title | Patterns of storage protein and triacylglycerol accumulation during loblolly pine somatic embryo maturation |
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