Preparation and immunological evaluation of inactivated avian influenza virus vaccine encapsulated in chitosan nanoparticles

Efficacy maximization of inactivated avian influenza vaccine using safe adjuvants was investigated. Chitosan nanoparticles were prepared by ionic gelation method with average size of 150 nm and their Zeta potential was 11.5 mV. After encapsulation of avian influenza vaccine, the average size was 397...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Biologicals 2018-01, Vol.51, p.46-53
Hauptverfasser: Mohamed, Samah H., Arafa, Abdel Satar, Mady, Wesam H., Fahmy, Hanan A., Omer, Lamiaa M., Morsi, Rania E.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 53
container_issue
container_start_page 46
container_title Biologicals
container_volume 51
creator Mohamed, Samah H.
Arafa, Abdel Satar
Mady, Wesam H.
Fahmy, Hanan A.
Omer, Lamiaa M.
Morsi, Rania E.
description Efficacy maximization of inactivated avian influenza vaccine using safe adjuvants was investigated. Chitosan nanoparticles were prepared by ionic gelation method with average size of 150 nm and their Zeta potential was 11.5 mV. After encapsulation of avian influenza vaccine, the average size was 397 nm and Zeta potential was 4.29 mV. The highest HI antibody titer results were shown in chicken group vaccinated with inactivated avian influenza virus AIV-chitosan followed by the group vaccinated with inactivated AIV-chitosan nanoparticles then the group vaccinated with oil inactivated AIV vaccine, on using chicken antigen at 2 weeks post second vaccination. Upon using duck antigen, the highest HI antibody titers were shown in chicken group vaccinated with inactivated AIV oil emulsion vaccine followed by chicken group vaccinated with AIV-chitosan nanoparticles then the group vaccinated with AIV–chitosan. Chicken in the group vaccinated with AIV-chitosan nanoparticles induced the best results of lymphocyte proliferation assay. The results of phagocytic activity percentage and phagocytic index of AIV-chitosan nanoparticles and AIV–chitosan groups at 3 days post first vaccination were increased significantly in comparison with other groups, whereas at 14 days post first vaccination, group vaccinated with AIV-chitosan nanoparticles showed significant increase in phagocytic activity percentage and phagocytic index. [Display omitted]
doi_str_mv 10.1016/j.biologicals.2017.10.004
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1963270796</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1045105617301306</els_id><sourcerecordid>1963270796</sourcerecordid><originalsourceid>FETCH-LOGICAL-c447t-3a42a15466b45f245133ee994b94b74edf2b440437f31c4832ed51e9de90f8643</originalsourceid><addsrcrecordid>eNqNUE1r3DAQFaUlH9v8haDcevFGssd2fAxLmwQC6aE9i7E8TmaxpY1kGxr646vtJqHHCsEMM-_N4z0hLrRaa6Wry-26ZT_4R7Y4xHWudJ3ma6XggzjRqimzqyJXH_c9lJlWZXUsTmPcKqU11HAkjvNG51V6J-L390A7DDixdxJdJ3kcZ_d2XNKCw3xY-l6yQzvxghN1EhdGlyb9MJN7QblwmKNc0Fp2JMlZ3MV5-AtlJ-0TTz4mgkPnk97EdqD4WXzqkwM6e60r8fPb1x-b2-z-4eZuc32fWYB6ygqEHHUJVdVC2edQ6qIgahpo06-Buj5vARQUdV9oC8k8daWmpqNG9VcVFCvx5XB3F_zzTHEyI0dLw4CO_ByNbqoir1Wdyko0B6gNPsZAvdkFHjH8MlqZffhma_4J3-zD369S-Il7_ioztyN178y3tBNgcwBQMrswBRMtp6io40B2Mp3n_5D5A86anrg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1963270796</pqid></control><display><type>article</type><title>Preparation and immunological evaluation of inactivated avian influenza virus vaccine encapsulated in chitosan nanoparticles</title><source>Elsevier ScienceDirect Journals Complete</source><creator>Mohamed, Samah H. ; Arafa, Abdel Satar ; Mady, Wesam H. ; Fahmy, Hanan A. ; Omer, Lamiaa M. ; Morsi, Rania E.</creator><creatorcontrib>Mohamed, Samah H. ; Arafa, Abdel Satar ; Mady, Wesam H. ; Fahmy, Hanan A. ; Omer, Lamiaa M. ; Morsi, Rania E.</creatorcontrib><description>Efficacy maximization of inactivated avian influenza vaccine using safe adjuvants was investigated. Chitosan nanoparticles were prepared by ionic gelation method with average size of 150 nm and their Zeta potential was 11.5 mV. After encapsulation of avian influenza vaccine, the average size was 397 nm and Zeta potential was 4.29 mV. The highest HI antibody titer results were shown in chicken group vaccinated with inactivated avian influenza virus AIV-chitosan followed by the group vaccinated with inactivated AIV-chitosan nanoparticles then the group vaccinated with oil inactivated AIV vaccine, on using chicken antigen at 2 weeks post second vaccination. Upon using duck antigen, the highest HI antibody titers were shown in chicken group vaccinated with inactivated AIV oil emulsion vaccine followed by chicken group vaccinated with AIV-chitosan nanoparticles then the group vaccinated with AIV–chitosan. Chicken in the group vaccinated with AIV-chitosan nanoparticles induced the best results of lymphocyte proliferation assay. The results of phagocytic activity percentage and phagocytic index of AIV-chitosan nanoparticles and AIV–chitosan groups at 3 days post first vaccination were increased significantly in comparison with other groups, whereas at 14 days post first vaccination, group vaccinated with AIV-chitosan nanoparticles showed significant increase in phagocytic activity percentage and phagocytic index. [Display omitted]</description><identifier>ISSN: 1045-1056</identifier><identifier>EISSN: 1095-8320</identifier><identifier>DOI: 10.1016/j.biologicals.2017.10.004</identifier><identifier>PMID: 29126666</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Avian influenza virus ; Chitosan nanoparticles ; Humoral and cell mediated immunity</subject><ispartof>Biologicals, 2018-01, Vol.51, p.46-53</ispartof><rights>2017 International Alliance for Biological Standardization</rights><rights>Copyright © 2017 International Alliance for Biological Standardization. Published by Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-3a42a15466b45f245133ee994b94b74edf2b440437f31c4832ed51e9de90f8643</citedby><cites>FETCH-LOGICAL-c447t-3a42a15466b45f245133ee994b94b74edf2b440437f31c4832ed51e9de90f8643</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biologicals.2017.10.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29126666$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mohamed, Samah H.</creatorcontrib><creatorcontrib>Arafa, Abdel Satar</creatorcontrib><creatorcontrib>Mady, Wesam H.</creatorcontrib><creatorcontrib>Fahmy, Hanan A.</creatorcontrib><creatorcontrib>Omer, Lamiaa M.</creatorcontrib><creatorcontrib>Morsi, Rania E.</creatorcontrib><title>Preparation and immunological evaluation of inactivated avian influenza virus vaccine encapsulated in chitosan nanoparticles</title><title>Biologicals</title><addtitle>Biologicals</addtitle><description>Efficacy maximization of inactivated avian influenza vaccine using safe adjuvants was investigated. Chitosan nanoparticles were prepared by ionic gelation method with average size of 150 nm and their Zeta potential was 11.5 mV. After encapsulation of avian influenza vaccine, the average size was 397 nm and Zeta potential was 4.29 mV. The highest HI antibody titer results were shown in chicken group vaccinated with inactivated avian influenza virus AIV-chitosan followed by the group vaccinated with inactivated AIV-chitosan nanoparticles then the group vaccinated with oil inactivated AIV vaccine, on using chicken antigen at 2 weeks post second vaccination. Upon using duck antigen, the highest HI antibody titers were shown in chicken group vaccinated with inactivated AIV oil emulsion vaccine followed by chicken group vaccinated with AIV-chitosan nanoparticles then the group vaccinated with AIV–chitosan. Chicken in the group vaccinated with AIV-chitosan nanoparticles induced the best results of lymphocyte proliferation assay. The results of phagocytic activity percentage and phagocytic index of AIV-chitosan nanoparticles and AIV–chitosan groups at 3 days post first vaccination were increased significantly in comparison with other groups, whereas at 14 days post first vaccination, group vaccinated with AIV-chitosan nanoparticles showed significant increase in phagocytic activity percentage and phagocytic index. [Display omitted]</description><subject>Avian influenza virus</subject><subject>Chitosan nanoparticles</subject><subject>Humoral and cell mediated immunity</subject><issn>1045-1056</issn><issn>1095-8320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNqNUE1r3DAQFaUlH9v8haDcevFGssd2fAxLmwQC6aE9i7E8TmaxpY1kGxr646vtJqHHCsEMM-_N4z0hLrRaa6Wry-26ZT_4R7Y4xHWudJ3ma6XggzjRqimzqyJXH_c9lJlWZXUsTmPcKqU11HAkjvNG51V6J-L390A7DDixdxJdJ3kcZ_d2XNKCw3xY-l6yQzvxghN1EhdGlyb9MJN7QblwmKNc0Fp2JMlZ3MV5-AtlJ-0TTz4mgkPnk97EdqD4WXzqkwM6e60r8fPb1x-b2-z-4eZuc32fWYB6ygqEHHUJVdVC2edQ6qIgahpo06-Buj5vARQUdV9oC8k8daWmpqNG9VcVFCvx5XB3F_zzTHEyI0dLw4CO_ByNbqoir1Wdyko0B6gNPsZAvdkFHjH8MlqZffhma_4J3-zD369S-Il7_ioztyN178y3tBNgcwBQMrswBRMtp6io40B2Mp3n_5D5A86anrg</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Mohamed, Samah H.</creator><creator>Arafa, Abdel Satar</creator><creator>Mady, Wesam H.</creator><creator>Fahmy, Hanan A.</creator><creator>Omer, Lamiaa M.</creator><creator>Morsi, Rania E.</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201801</creationdate><title>Preparation and immunological evaluation of inactivated avian influenza virus vaccine encapsulated in chitosan nanoparticles</title><author>Mohamed, Samah H. ; Arafa, Abdel Satar ; Mady, Wesam H. ; Fahmy, Hanan A. ; Omer, Lamiaa M. ; Morsi, Rania E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-3a42a15466b45f245133ee994b94b74edf2b440437f31c4832ed51e9de90f8643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Avian influenza virus</topic><topic>Chitosan nanoparticles</topic><topic>Humoral and cell mediated immunity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mohamed, Samah H.</creatorcontrib><creatorcontrib>Arafa, Abdel Satar</creatorcontrib><creatorcontrib>Mady, Wesam H.</creatorcontrib><creatorcontrib>Fahmy, Hanan A.</creatorcontrib><creatorcontrib>Omer, Lamiaa M.</creatorcontrib><creatorcontrib>Morsi, Rania E.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biologicals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mohamed, Samah H.</au><au>Arafa, Abdel Satar</au><au>Mady, Wesam H.</au><au>Fahmy, Hanan A.</au><au>Omer, Lamiaa M.</au><au>Morsi, Rania E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and immunological evaluation of inactivated avian influenza virus vaccine encapsulated in chitosan nanoparticles</atitle><jtitle>Biologicals</jtitle><addtitle>Biologicals</addtitle><date>2018-01</date><risdate>2018</risdate><volume>51</volume><spage>46</spage><epage>53</epage><pages>46-53</pages><issn>1045-1056</issn><eissn>1095-8320</eissn><abstract>Efficacy maximization of inactivated avian influenza vaccine using safe adjuvants was investigated. Chitosan nanoparticles were prepared by ionic gelation method with average size of 150 nm and their Zeta potential was 11.5 mV. After encapsulation of avian influenza vaccine, the average size was 397 nm and Zeta potential was 4.29 mV. The highest HI antibody titer results were shown in chicken group vaccinated with inactivated avian influenza virus AIV-chitosan followed by the group vaccinated with inactivated AIV-chitosan nanoparticles then the group vaccinated with oil inactivated AIV vaccine, on using chicken antigen at 2 weeks post second vaccination. Upon using duck antigen, the highest HI antibody titers were shown in chicken group vaccinated with inactivated AIV oil emulsion vaccine followed by chicken group vaccinated with AIV-chitosan nanoparticles then the group vaccinated with AIV–chitosan. Chicken in the group vaccinated with AIV-chitosan nanoparticles induced the best results of lymphocyte proliferation assay. The results of phagocytic activity percentage and phagocytic index of AIV-chitosan nanoparticles and AIV–chitosan groups at 3 days post first vaccination were increased significantly in comparison with other groups, whereas at 14 days post first vaccination, group vaccinated with AIV-chitosan nanoparticles showed significant increase in phagocytic activity percentage and phagocytic index. [Display omitted]</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29126666</pmid><doi>10.1016/j.biologicals.2017.10.004</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1045-1056
ispartof Biologicals, 2018-01, Vol.51, p.46-53
issn 1045-1056
1095-8320
language eng
recordid cdi_proquest_miscellaneous_1963270796
source Elsevier ScienceDirect Journals Complete
subjects Avian influenza virus
Chitosan nanoparticles
Humoral and cell mediated immunity
title Preparation and immunological evaluation of inactivated avian influenza virus vaccine encapsulated in chitosan nanoparticles
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T09%3A19%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20immunological%20evaluation%20of%20inactivated%20avian%20influenza%20virus%20vaccine%20encapsulated%20in%20chitosan%20nanoparticles&rft.jtitle=Biologicals&rft.au=Mohamed,%20Samah%20H.&rft.date=2018-01&rft.volume=51&rft.spage=46&rft.epage=53&rft.pages=46-53&rft.issn=1045-1056&rft.eissn=1095-8320&rft_id=info:doi/10.1016/j.biologicals.2017.10.004&rft_dat=%3Cproquest_cross%3E1963270796%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1963270796&rft_id=info:pmid/29126666&rft_els_id=S1045105617301306&rfr_iscdi=true