Region-specific glial hyperplasia and neuronal stability of rat lateral geniculate nucleus during aging
The normal aging process is accompanied by functional declines in image-forming and non-image forming visual systems. Among the components of these systems, the thalamic lateral geniculate nucleus (LGN) offers a good model for aging studies since its three anatomical subdivisions, namely dorsal late...
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| Veröffentlicht in: | Experimental gerontology 2017-12, Vol.100, p.91-99 |
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| creator | Fiuza, Felipe P. Aquino, Antônio Carlos Q. Câmara, Diego A. Cavalcanti, José Rodolfo L.P. Nascimento Júnior, Expedito S. Lima, Ramon H. Engelberth, Rovena Clara G.J. Cavalcante, Jeferson S. |
| description | The normal aging process is accompanied by functional declines in image-forming and non-image forming visual systems. Among the components of these systems, the thalamic lateral geniculate nucleus (LGN) offers a good model for aging studies since its three anatomical subdivisions, namely dorsal lateral geniculate nucleus (dLGN), intergeniculate leaflet (IGL) and ventral lateral geniculate nucleus (vLGN), receives light information from retina and projects to different brain areas involved in visual-related functions. Nevertheless, there is very little data available about quantitative morphological aspects in LGN across lifespan. In this study, we used design-based stereology to estimate the number of neurons, glial cells, the glia/neuron ratio and the volume of the LGN of Wistar rats from 3, 13 or 23months of age. We examined each LGN subdivision processed by immunohistochemistry for NeuN and Nissl counterstain. We observed no significant age-related neuronal loss in any nuclei and a 21% and 33% significant increase in dLGN and IGL glial cells of 23month-old rats. We also observed the glia/neuron relation increases in dLGN of 13month-old rats and in dLGN, IGL and vLGN internal portion of 23month-old ones. Moreover, we report an age-related increase in IGL volume. These results show region-specific glial hyperplasia during aging within LGN nuclei, perhaps due to compensatory responses to inflammation. In addition, we observed the glia/neuron ratio as a more sensitive parameter to quantify age-related alterations. Hence, we provide an updated and expanded quantitative characterization of these visual-related thalamic nuclei and its variability across lifespan.
•Quantitative morphological parameters in LGN are described across the rat lifespan.•There are no age-related alterations in number of neurons of rat LGN.•In dLGN and IGL the glial cell number increases in 23months-old rats.•The IGL volume increases in 23months-old rats.•The glia/neuron ratio is a more sensitive parameter for aging changes in rat LGN. |
| doi_str_mv | 10.1016/j.exger.2017.11.001 |
| format | Article |
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•Quantitative morphological parameters in LGN are described across the rat lifespan.•There are no age-related alterations in number of neurons of rat LGN.•In dLGN and IGL the glial cell number increases in 23months-old rats.•The IGL volume increases in 23months-old rats.•The glia/neuron ratio is a more sensitive parameter for aging changes in rat LGN.</description><identifier>ISSN: 0531-5565</identifier><identifier>EISSN: 1873-6815</identifier><identifier>DOI: 10.1016/j.exger.2017.11.001</identifier><identifier>PMID: 29113752</identifier><language>eng</language><publisher>England: Elsevier Inc</publisher><subject>Aging ; Animals ; Geniculate Bodies - pathology ; Glia/neuron ratio ; Hyperplasia - pathology ; Immunohistochemistry ; Lateral geniculate nucleus ; Male ; NeuN ; Neuroglia - cytology ; Neurons - cytology ; Rats ; Rats, Wistar ; Stereology</subject><ispartof>Experimental gerontology, 2017-12, Vol.100, p.91-99</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-2eb3bb826a25e4a9f6fc6cf959bde910305d8dead74e64667c47bb14391e3dc03</citedby><cites>FETCH-LOGICAL-c359t-2eb3bb826a25e4a9f6fc6cf959bde910305d8dead74e64667c47bb14391e3dc03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S053155651730551X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29113752$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fiuza, Felipe P.</creatorcontrib><creatorcontrib>Aquino, Antônio Carlos Q.</creatorcontrib><creatorcontrib>Câmara, Diego A.</creatorcontrib><creatorcontrib>Cavalcanti, José Rodolfo L.P.</creatorcontrib><creatorcontrib>Nascimento Júnior, Expedito S.</creatorcontrib><creatorcontrib>Lima, Ramon H.</creatorcontrib><creatorcontrib>Engelberth, Rovena Clara G.J.</creatorcontrib><creatorcontrib>Cavalcante, Jeferson S.</creatorcontrib><title>Region-specific glial hyperplasia and neuronal stability of rat lateral geniculate nucleus during aging</title><title>Experimental gerontology</title><addtitle>Exp Gerontol</addtitle><description>The normal aging process is accompanied by functional declines in image-forming and non-image forming visual systems. Among the components of these systems, the thalamic lateral geniculate nucleus (LGN) offers a good model for aging studies since its three anatomical subdivisions, namely dorsal lateral geniculate nucleus (dLGN), intergeniculate leaflet (IGL) and ventral lateral geniculate nucleus (vLGN), receives light information from retina and projects to different brain areas involved in visual-related functions. Nevertheless, there is very little data available about quantitative morphological aspects in LGN across lifespan. In this study, we used design-based stereology to estimate the number of neurons, glial cells, the glia/neuron ratio and the volume of the LGN of Wistar rats from 3, 13 or 23months of age. We examined each LGN subdivision processed by immunohistochemistry for NeuN and Nissl counterstain. We observed no significant age-related neuronal loss in any nuclei and a 21% and 33% significant increase in dLGN and IGL glial cells of 23month-old rats. We also observed the glia/neuron relation increases in dLGN of 13month-old rats and in dLGN, IGL and vLGN internal portion of 23month-old ones. Moreover, we report an age-related increase in IGL volume. These results show region-specific glial hyperplasia during aging within LGN nuclei, perhaps due to compensatory responses to inflammation. In addition, we observed the glia/neuron ratio as a more sensitive parameter to quantify age-related alterations. Hence, we provide an updated and expanded quantitative characterization of these visual-related thalamic nuclei and its variability across lifespan.
•Quantitative morphological parameters in LGN are described across the rat lifespan.•There are no age-related alterations in number of neurons of rat LGN.•In dLGN and IGL the glial cell number increases in 23months-old rats.•The IGL volume increases in 23months-old rats.•The glia/neuron ratio is a more sensitive parameter for aging changes in rat LGN.</description><subject>Aging</subject><subject>Animals</subject><subject>Geniculate Bodies - pathology</subject><subject>Glia/neuron ratio</subject><subject>Hyperplasia - pathology</subject><subject>Immunohistochemistry</subject><subject>Lateral geniculate nucleus</subject><subject>Male</subject><subject>NeuN</subject><subject>Neuroglia - cytology</subject><subject>Neurons - cytology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Stereology</subject><issn>0531-5565</issn><issn>1873-6815</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9r3DAQxUVpaDZ_PkGh6NiLHY1lyfYhhxLSJBAIhPYsZGnsatHKjmSX7Levtpv2mMsMw7w3j_kR8hlYCQzk1bbE1xFjWTFoSoCSMfhANtA2vJAtiI9kwwSHQggpTslZSlvGmKw4fCKnVQfAG1FtyPiMo5tCkWY0bnCGjt5pT3_tZ4yz18lpqoOlAdc4hbxIi-6dd8ueTgONeqFeLxjzYsTgzHqYaFiNxzVRu0YXRqrHXC_IyaB9wsu3fk5-fr_9cXNfPD7dPdx8eywMF91SVNjzvm8rqSuBte4GORhphk50vcUOGGfCtha1bWqUtZSNqZu-h5p3gNwaxs_J1-PdOU4vK6ZF7Vwy6L0OOK1JQSehFQxEk6X8KDVxSinioObodjruFTB1IKy26i9hdSCsAFQmnF1f3gLWfof2v-cf0iy4Pgowv_nbZXsyDoNB6yKaRdnJvRvwB0aej8A</recordid><startdate>20171215</startdate><enddate>20171215</enddate><creator>Fiuza, Felipe P.</creator><creator>Aquino, Antônio Carlos Q.</creator><creator>Câmara, Diego A.</creator><creator>Cavalcanti, José Rodolfo L.P.</creator><creator>Nascimento Júnior, Expedito S.</creator><creator>Lima, Ramon H.</creator><creator>Engelberth, Rovena Clara G.J.</creator><creator>Cavalcante, Jeferson S.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20171215</creationdate><title>Region-specific glial hyperplasia and neuronal stability of rat lateral geniculate nucleus during aging</title><author>Fiuza, Felipe P. ; Aquino, Antônio Carlos Q. ; Câmara, Diego A. ; Cavalcanti, José Rodolfo L.P. ; Nascimento Júnior, Expedito S. ; Lima, Ramon H. ; Engelberth, Rovena Clara G.J. ; Cavalcante, Jeferson S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-2eb3bb826a25e4a9f6fc6cf959bde910305d8dead74e64667c47bb14391e3dc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Aging</topic><topic>Animals</topic><topic>Geniculate Bodies - pathology</topic><topic>Glia/neuron ratio</topic><topic>Hyperplasia - pathology</topic><topic>Immunohistochemistry</topic><topic>Lateral geniculate nucleus</topic><topic>Male</topic><topic>NeuN</topic><topic>Neuroglia - cytology</topic><topic>Neurons - cytology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Stereology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fiuza, Felipe P.</creatorcontrib><creatorcontrib>Aquino, Antônio Carlos Q.</creatorcontrib><creatorcontrib>Câmara, Diego A.</creatorcontrib><creatorcontrib>Cavalcanti, José Rodolfo L.P.</creatorcontrib><creatorcontrib>Nascimento Júnior, Expedito S.</creatorcontrib><creatorcontrib>Lima, Ramon H.</creatorcontrib><creatorcontrib>Engelberth, Rovena Clara G.J.</creatorcontrib><creatorcontrib>Cavalcante, Jeferson S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental gerontology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fiuza, Felipe P.</au><au>Aquino, Antônio Carlos Q.</au><au>Câmara, Diego A.</au><au>Cavalcanti, José Rodolfo L.P.</au><au>Nascimento Júnior, Expedito S.</au><au>Lima, Ramon H.</au><au>Engelberth, Rovena Clara G.J.</au><au>Cavalcante, Jeferson S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Region-specific glial hyperplasia and neuronal stability of rat lateral geniculate nucleus during aging</atitle><jtitle>Experimental gerontology</jtitle><addtitle>Exp Gerontol</addtitle><date>2017-12-15</date><risdate>2017</risdate><volume>100</volume><spage>91</spage><epage>99</epage><pages>91-99</pages><issn>0531-5565</issn><eissn>1873-6815</eissn><abstract>The normal aging process is accompanied by functional declines in image-forming and non-image forming visual systems. Among the components of these systems, the thalamic lateral geniculate nucleus (LGN) offers a good model for aging studies since its three anatomical subdivisions, namely dorsal lateral geniculate nucleus (dLGN), intergeniculate leaflet (IGL) and ventral lateral geniculate nucleus (vLGN), receives light information from retina and projects to different brain areas involved in visual-related functions. Nevertheless, there is very little data available about quantitative morphological aspects in LGN across lifespan. In this study, we used design-based stereology to estimate the number of neurons, glial cells, the glia/neuron ratio and the volume of the LGN of Wistar rats from 3, 13 or 23months of age. We examined each LGN subdivision processed by immunohistochemistry for NeuN and Nissl counterstain. We observed no significant age-related neuronal loss in any nuclei and a 21% and 33% significant increase in dLGN and IGL glial cells of 23month-old rats. We also observed the glia/neuron relation increases in dLGN of 13month-old rats and in dLGN, IGL and vLGN internal portion of 23month-old ones. Moreover, we report an age-related increase in IGL volume. These results show region-specific glial hyperplasia during aging within LGN nuclei, perhaps due to compensatory responses to inflammation. In addition, we observed the glia/neuron ratio as a more sensitive parameter to quantify age-related alterations. Hence, we provide an updated and expanded quantitative characterization of these visual-related thalamic nuclei and its variability across lifespan.
•Quantitative morphological parameters in LGN are described across the rat lifespan.•There are no age-related alterations in number of neurons of rat LGN.•In dLGN and IGL the glial cell number increases in 23months-old rats.•The IGL volume increases in 23months-old rats.•The glia/neuron ratio is a more sensitive parameter for aging changes in rat LGN.</abstract><cop>England</cop><pub>Elsevier Inc</pub><pmid>29113752</pmid><doi>10.1016/j.exger.2017.11.001</doi><tpages>9</tpages></addata></record> |
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| ispartof | Experimental gerontology, 2017-12, Vol.100, p.91-99 |
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| subjects | Aging Animals Geniculate Bodies - pathology Glia/neuron ratio Hyperplasia - pathology Immunohistochemistry Lateral geniculate nucleus Male NeuN Neuroglia - cytology Neurons - cytology Rats Rats, Wistar Stereology |
| title | Region-specific glial hyperplasia and neuronal stability of rat lateral geniculate nucleus during aging |
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