Glycan profile of CHO derived IgM purified by highly efficient single step affinity chromatography

Immunoglobulin M (IgM) antibodies are reckoned as promising tools for therapy and diagnostic approaches. Nevertheless, the commercial success of IgMs is hampered due to bottlenecks in recombinant production and downstream processing. IgMs are large, complex and highly glycosylated proteins that are...

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Veröffentlicht in:	Analytical biochemistry 2017-12, Vol.539, p.162-166
Hauptverfasser:	Hennicke, Julia, Lastin, Anna Maria, Reinhart, David, Grünwald-Gruber, Clemens, Altmann, Friedrich, Kunert, Renate
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity purification Animals Chinese hamster ovary (CHO) CHO Cells Chromatography, Affinity Cricetinae Cricetulus Downstream processing Electrophoresis, Polyacrylamide Gel Glycosylation Hydrogen-Ion Concentration IgM Immunoglobulin M - biosynthesis Immunoglobulin M - chemistry Immunoglobulin M - isolation & purification Oligosaccharides - chemistry pH stability Polysaccharides - chemistry Protein Stability Site-specific glycosylation
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