A hexahistidine-Zn super(2+)-dye label reveals STIM1 surface exposure

A hexahistidine-Zn super(2+)-dye label reveals STIM1 surface exposure

Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large t...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 2007-03, Vol.104 (10), p.3693-3697
Hauptverfasser: Hauser, Christina T, Tsien, Roger Y
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Tsien, Roger Y
description Site-specific fluorescent labeling of proteins in vivo remains one of the most powerful techniques for imaging complex processes in live cells. Although fluorescent proteins in many colors are useful tools for tracking expression and localization of fusion proteins in cells, these relatively large tags (>220 aa) can perturb protein folding, trafficking and function. Much smaller genetically encodable domains (
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title A hexahistidine-Zn super(2+)-dye label reveals STIM1 surface exposure
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