Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage
The World Health Organization acknowledges tuberculosis as a global threat. Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tu...
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| Veröffentlicht in: | Journal of basic microbiology 2018-01, Vol.58 (1), p.41-51 |
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| creator | Jafari, Alireza Jafari Nodooshan, Saeedeh Safarkar, Roya Movahedzadeh, Farahnaz Mosavari, Nader Novin Kashani, Aren Dehghanpour, Mahdi Kamalzadeh, Morteza Rasouli Koohi, Samira Fathizadeh, Sara Majidpour, Ali |
| description | The World Health Organization acknowledges tuberculosis as a global threat. Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano‐crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared. The MTT assay was employed to study the viability of human macrophage cell lines (THP‐1) exposed to AgZnONCs. The bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were studied by Minimum Bactericidal Concentration (MBC) test. Subsequently, THP‐1 were infected by H37Rv strain of M. tuberculosis (H37RvMtb). Also, bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were compared with ex‐vivo conditions. The MBC of AgZnONCs and AgZnONCs+Rifampicin were ratios of 1:4 and 1:32 respectively (p‐value |
| doi_str_mv | 10.1002/jobm.201700289 |
| format | Article |
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Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano‐crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared. The MTT assay was employed to study the viability of human macrophage cell lines (THP‐1) exposed to AgZnONCs. The bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were studied by Minimum Bactericidal Concentration (MBC) test. Subsequently, THP‐1 were infected by H37Rv strain of M. tuberculosis (H37RvMtb). Also, bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were compared with ex‐vivo conditions. The MBC of AgZnONCs and AgZnONCs+Rifampicin were ratios of 1:4 and 1:32 respectively (p‐value <0.05). Also, more than 50% and 80% of THP‐1 were alive in ratios of 1:4 and 1:32 in the presence of AgZnONCs, respectively. All phagocytic H37RvMtb were killed in the presence of AgZnONCs+Rifampicin (p‐value <0.05), while AgZnONCs were not able to kill all the H37RvMtb (p‐value >0.05). This study showed that, AgZnONCs+Rifampicin has the most anti‐tubercular behavior with respect to the macrophages.</description><identifier>ISSN: 0233-111X</identifier><identifier>EISSN: 1521-4028</identifier><identifier>DOI: 10.1002/jobm.201700289</identifier><identifier>PMID: 29105782</identifier><language>eng</language><publisher>Germany</publisher><subject>AgZnO nano‐crystals ; Antitubercular Agents - toxicity ; bactericidal ; Cell Line ; Cell Survival - drug effects ; Humans ; macrophage ; Macrophages - drug effects ; Macrophages - microbiology ; Microbial Sensitivity Tests ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - drug effects ; Nanoparticles - chemistry ; Phagocytosis ; rifampicin ; Rifampin - toxicity ; Silver - toxicity ; Zinc Oxide - toxicity</subject><ispartof>Journal of basic microbiology, 2018-01, Vol.58 (1), p.41-51</ispartof><rights>2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3459-848db60a34a1350964ad729b0dd0d082085820288d6add7594b39665ada831763</citedby><cites>FETCH-LOGICAL-c3459-848db60a34a1350964ad729b0dd0d082085820288d6add7594b39665ada831763</cites><orcidid>0000-0003-1523-2691</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjobm.201700289$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjobm.201700289$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29105782$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jafari, Alireza</creatorcontrib><creatorcontrib>Jafari Nodooshan, Saeedeh</creatorcontrib><creatorcontrib>Safarkar, Roya</creatorcontrib><creatorcontrib>Movahedzadeh, Farahnaz</creatorcontrib><creatorcontrib>Mosavari, Nader</creatorcontrib><creatorcontrib>Novin Kashani, Aren</creatorcontrib><creatorcontrib>Dehghanpour, Mahdi</creatorcontrib><creatorcontrib>Kamalzadeh, Morteza</creatorcontrib><creatorcontrib>Rasouli Koohi, Samira</creatorcontrib><creatorcontrib>Fathizadeh, Sara</creatorcontrib><creatorcontrib>Majidpour, Ali</creatorcontrib><title>Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage</title><title>Journal of basic microbiology</title><addtitle>J Basic Microbiol</addtitle><description>The World Health Organization acknowledges tuberculosis as a global threat. Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano‐crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared. The MTT assay was employed to study the viability of human macrophage cell lines (THP‐1) exposed to AgZnONCs. The bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were studied by Minimum Bactericidal Concentration (MBC) test. Subsequently, THP‐1 were infected by H37Rv strain of M. tuberculosis (H37RvMtb). Also, bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were compared with ex‐vivo conditions. The MBC of AgZnONCs and AgZnONCs+Rifampicin were ratios of 1:4 and 1:32 respectively (p‐value <0.05). Also, more than 50% and 80% of THP‐1 were alive in ratios of 1:4 and 1:32 in the presence of AgZnONCs, respectively. All phagocytic H37RvMtb were killed in the presence of AgZnONCs+Rifampicin (p‐value <0.05), while AgZnONCs were not able to kill all the H37RvMtb (p‐value >0.05). This study showed that, AgZnONCs+Rifampicin has the most anti‐tubercular behavior with respect to the macrophages.</description><subject>AgZnO nano‐crystals</subject><subject>Antitubercular Agents - toxicity</subject><subject>bactericidal</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Humans</subject><subject>macrophage</subject><subject>Macrophages - drug effects</subject><subject>Macrophages - microbiology</subject><subject>Microbial Sensitivity Tests</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - drug effects</subject><subject>Nanoparticles - chemistry</subject><subject>Phagocytosis</subject><subject>rifampicin</subject><subject>Rifampin - toxicity</subject><subject>Silver - toxicity</subject><subject>Zinc Oxide - toxicity</subject><issn>0233-111X</issn><issn>1521-4028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EoqWwMiKPLClnOx_2WCo-VdQFJMQSObZDXSVxsBNB_z2pCmVkudMrPffq9CB0TmBKAOjV2hX1lALJhsDFARqThJIoHsIhGgNlLCKEvI7QSQhrABCCimM0ooJAknE6Rqtn92WV7TbYlKVRXcCuxLP3t2aJG9m4VvrOqsoELBuNvS1l3Q54g12DnzbKFVJ1xtu-xl1fGK_6ygUbsG06h7uVwbVU3rUr-W5O0VEpq2DOfvYEvdzePM_vo8Xy7mE-W0SKxYmIeMx1kYJksSQsAZHGUmdUFKA1aOAUeDIMyrlOpdZZIuKCiTRNpJackSxlE3S56229--hN6PLaBmWqSjbG9SEnIiXAspgmAzrdocOPIXhT5q23tfSbnEC-tZtv7eZ7u8PBxU93X9RG7_FfnQMgdsCnrczmn7r8cXn99Ff-DU-ahzE</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Jafari, Alireza</creator><creator>Jafari Nodooshan, Saeedeh</creator><creator>Safarkar, Roya</creator><creator>Movahedzadeh, Farahnaz</creator><creator>Mosavari, Nader</creator><creator>Novin Kashani, Aren</creator><creator>Dehghanpour, Mahdi</creator><creator>Kamalzadeh, Morteza</creator><creator>Rasouli Koohi, Samira</creator><creator>Fathizadeh, Sara</creator><creator>Majidpour, Ali</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1523-2691</orcidid></search><sort><creationdate>201801</creationdate><title>Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage</title><author>Jafari, Alireza ; Jafari Nodooshan, Saeedeh ; Safarkar, Roya ; Movahedzadeh, Farahnaz ; Mosavari, Nader ; Novin Kashani, Aren ; Dehghanpour, Mahdi ; Kamalzadeh, Morteza ; Rasouli Koohi, Samira ; Fathizadeh, Sara ; Majidpour, Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3459-848db60a34a1350964ad729b0dd0d082085820288d6add7594b39665ada831763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>AgZnO nano‐crystals</topic><topic>Antitubercular Agents - toxicity</topic><topic>bactericidal</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Humans</topic><topic>macrophage</topic><topic>Macrophages - drug effects</topic><topic>Macrophages - microbiology</topic><topic>Microbial Sensitivity Tests</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - drug effects</topic><topic>Nanoparticles - chemistry</topic><topic>Phagocytosis</topic><topic>rifampicin</topic><topic>Rifampin - toxicity</topic><topic>Silver - toxicity</topic><topic>Zinc Oxide - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jafari, Alireza</creatorcontrib><creatorcontrib>Jafari Nodooshan, Saeedeh</creatorcontrib><creatorcontrib>Safarkar, Roya</creatorcontrib><creatorcontrib>Movahedzadeh, Farahnaz</creatorcontrib><creatorcontrib>Mosavari, Nader</creatorcontrib><creatorcontrib>Novin Kashani, Aren</creatorcontrib><creatorcontrib>Dehghanpour, Mahdi</creatorcontrib><creatorcontrib>Kamalzadeh, Morteza</creatorcontrib><creatorcontrib>Rasouli Koohi, Samira</creatorcontrib><creatorcontrib>Fathizadeh, Sara</creatorcontrib><creatorcontrib>Majidpour, Ali</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of basic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jafari, Alireza</au><au>Jafari Nodooshan, Saeedeh</au><au>Safarkar, Roya</au><au>Movahedzadeh, Farahnaz</au><au>Mosavari, Nader</au><au>Novin Kashani, Aren</au><au>Dehghanpour, Mahdi</au><au>Kamalzadeh, Morteza</au><au>Rasouli Koohi, Samira</au><au>Fathizadeh, Sara</au><au>Majidpour, Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage</atitle><jtitle>Journal of basic microbiology</jtitle><addtitle>J Basic Microbiol</addtitle><date>2018-01</date><risdate>2018</risdate><volume>58</volume><issue>1</issue><spage>41</spage><epage>51</epage><pages>41-51</pages><issn>0233-111X</issn><eissn>1521-4028</eissn><abstract>The World Health Organization acknowledges tuberculosis as a global threat. Tuberculosis infection is one of the top 10 causes of death worldwide. Nanotechnology and microbiology researchers are looking for new and safe nano drugs for eliminating Mycobacterium tuberculosis, the causative agent of tuberculosis. In this study, AgZnO nano‐crystals (AgZnONCs) is synthesized via the decomposition of the precursor of oxalate method. Characterization of AgZnONCs were evaluated. Next, various concentrations of AgZnONCs, as well AgZnONCs+Rifampicin, were prepared. The MTT assay was employed to study the viability of human macrophage cell lines (THP‐1) exposed to AgZnONCs. The bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were studied by Minimum Bactericidal Concentration (MBC) test. Subsequently, THP‐1 were infected by H37Rv strain of M. tuberculosis (H37RvMtb). Also, bactericidal effects of AgZnONCs and AgZnONCs+Rifampicin were compared with ex‐vivo conditions. The MBC of AgZnONCs and AgZnONCs+Rifampicin were ratios of 1:4 and 1:32 respectively (p‐value <0.05). Also, more than 50% and 80% of THP‐1 were alive in ratios of 1:4 and 1:32 in the presence of AgZnONCs, respectively. All phagocytic H37RvMtb were killed in the presence of AgZnONCs+Rifampicin (p‐value <0.05), while AgZnONCs were not able to kill all the H37RvMtb (p‐value >0.05). This study showed that, AgZnONCs+Rifampicin has the most anti‐tubercular behavior with respect to the macrophages.</abstract><cop>Germany</cop><pmid>29105782</pmid><doi>10.1002/jobm.201700289</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-1523-2691</orcidid></addata></record> |
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| subjects | AgZnO nano‐crystals Antitubercular Agents - toxicity bactericidal Cell Line Cell Survival - drug effects Humans macrophage Macrophages - drug effects Macrophages - microbiology Microbial Sensitivity Tests Mycobacterium tuberculosis Mycobacterium tuberculosis - drug effects Nanoparticles - chemistry Phagocytosis rifampicin Rifampin - toxicity Silver - toxicity Zinc Oxide - toxicity |
| title | Toxicity effects of AgZnO nanoparticles and rifampicin on Mycobacterium tuberculosis into the macrophage |
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