Enhanced oxygen permeability in membrane-bottomed concave microwells for the formation of pancreatic islet spheroids
[Display omitted] Oxygen availability is a critical factor in regulating cell viability that ultimately contributes to the normal morphogenesis and functionality of human tissues. Among various cell culture platforms, construction of 3D multicellular spheroids based on microwell arrays has been exte...
Gespeichert in:
| Veröffentlicht in: | Acta biomaterialia 2018-01, Vol.65, p.185-196 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 196 |
|---|---|
| container_issue | |
| container_start_page | 185 |
| container_title | Acta biomaterialia |
| container_volume | 65 |
| creator | Lee, GeonHui Jun, Yesl Jang, HeeYeong Yoon, Junghyo Lee, JaeSeo Hong, MinHyung Chung, Seok Kim, Dong-Hwee Lee, SangHoon |
| description | [Display omitted]
Oxygen availability is a critical factor in regulating cell viability that ultimately contributes to the normal morphogenesis and functionality of human tissues. Among various cell culture platforms, construction of 3D multicellular spheroids based on microwell arrays has been extensively applied to reconstitute in vitro human tissue models due to its precise control of tissue culture conditions as well as simple fabrication processes. However, an adequate supply of oxygen into the spheroidal cellular aggregation still remains one of the main challenges to producing healthy in vitro spheroidal tissue models. Here, we present a novel design for controlling the oxygen distribution in concave microwell arrays. We show that oxygen permeability into the microwell is tightly regulated by varying the poly-dimethylsiloxane (PDMS) bottom thickness of the concave microwells. Moreover, we validate the enhanced performance of the engineered microwell arrays by culturing non-proliferated primary rat pancreatic islet spheroids on varying bottom thickness from 10 μm to 1050 μm. Morphological and functional analyses performed on the pancreatic islet spheroids grown for 14 days prove the long-term stability, enhanced viability, and increased hormone secretion under the sufficient oxygen delivery conditions. We expect our results could provide knowledge on oxygen distribution in 3-dimensional spheroidal cell structures and critical design concept for tissue engineering applications.
In this study, we present a noble design to control the oxygen distribution in concave microwell arrays for the formation of highly functional pancreatic islet spheroids by engineering the bottom of the microwells. Our new platform significantly enhanced oxygen permeability that turned out to improve cell viability and spheroidal functionality compared to the conventional thick-bottomed 3-D culture system. Therefore, we believe that this could be a promising medical biotechnology platform to further develop high-throughput tissue screening system as well as in vivo-mimicking customised 3-D tissue culture systems. |
| doi_str_mv | 10.1016/j.actbio.2017.10.045 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1960929174</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1742706117306736</els_id><sourcerecordid>1988888822</sourcerecordid><originalsourceid>FETCH-LOGICAL-c427t-282131143a4f73e2a2bd0d7ec2662db91f851aa3f132b10924573049166573b23</originalsourceid><addsrcrecordid>eNp9UU1P3DAQtSpQobT_oEKWuPSSxeNk4-RSqVrRDwmpFzhbtjPpepXEwfZS9t930gUOPdSXGY_ee_PxGPsIYgUC6uvdyrhsfVhJAYpKK1Gt37BzaFRTqHXdnFCuKlkoUcMZe5fSToiyAdm8ZWeyJQminbN8M23N5LDj4enwCyc-YxzRWD_4fOB-4iOONpoJCxtyDiMBXZiceUQ-ehfDbxyGxPsQed7iEkeTfZh46PlMuhHp67hPA2ae5i3G4Lv0np32Zkj44TlesPuvN3eb78Xtz28_Nl9uC1dJlQvZSCgBqtJUvSpRGmk70Sl0sq5lZ1vomzUYU_ZQSguildValaJqoa4psbK8YJ-OunMMD3tMWY8-OZqY9gn7pKGtidXSmQh69Q90F_ZxoukI1fx9chGsjijaPKWIvZ6jH008aBB6cUXv9NEVvbiyVMkVol0-i-8tXfCV9GIDAT4fAUjXePQYdXIeF1t8RJd1F_z_O_wBT6Of4g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1988888822</pqid></control><display><type>article</type><title>Enhanced oxygen permeability in membrane-bottomed concave microwells for the formation of pancreatic islet spheroids</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Lee, GeonHui ; Jun, Yesl ; Jang, HeeYeong ; Yoon, Junghyo ; Lee, JaeSeo ; Hong, MinHyung ; Chung, Seok ; Kim, Dong-Hwee ; Lee, SangHoon</creator><creatorcontrib>Lee, GeonHui ; Jun, Yesl ; Jang, HeeYeong ; Yoon, Junghyo ; Lee, JaeSeo ; Hong, MinHyung ; Chung, Seok ; Kim, Dong-Hwee ; Lee, SangHoon</creatorcontrib><description>[Display omitted]
Oxygen availability is a critical factor in regulating cell viability that ultimately contributes to the normal morphogenesis and functionality of human tissues. Among various cell culture platforms, construction of 3D multicellular spheroids based on microwell arrays has been extensively applied to reconstitute in vitro human tissue models due to its precise control of tissue culture conditions as well as simple fabrication processes. However, an adequate supply of oxygen into the spheroidal cellular aggregation still remains one of the main challenges to producing healthy in vitro spheroidal tissue models. Here, we present a novel design for controlling the oxygen distribution in concave microwell arrays. We show that oxygen permeability into the microwell is tightly regulated by varying the poly-dimethylsiloxane (PDMS) bottom thickness of the concave microwells. Moreover, we validate the enhanced performance of the engineered microwell arrays by culturing non-proliferated primary rat pancreatic islet spheroids on varying bottom thickness from 10 μm to 1050 μm. Morphological and functional analyses performed on the pancreatic islet spheroids grown for 14 days prove the long-term stability, enhanced viability, and increased hormone secretion under the sufficient oxygen delivery conditions. We expect our results could provide knowledge on oxygen distribution in 3-dimensional spheroidal cell structures and critical design concept for tissue engineering applications.
In this study, we present a noble design to control the oxygen distribution in concave microwell arrays for the formation of highly functional pancreatic islet spheroids by engineering the bottom of the microwells. Our new platform significantly enhanced oxygen permeability that turned out to improve cell viability and spheroidal functionality compared to the conventional thick-bottomed 3-D culture system. Therefore, we believe that this could be a promising medical biotechnology platform to further develop high-throughput tissue screening system as well as in vivo-mimicking customised 3-D tissue culture systems.</description><identifier>ISSN: 1742-7061</identifier><identifier>EISSN: 1878-7568</identifier><identifier>DOI: 10.1016/j.actbio.2017.10.045</identifier><identifier>PMID: 29101017</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>3D spheroid ; Animal models ; Animals ; Arrays ; Cell culture ; Cells, Cultured ; Concave microwell ; Design engineering ; Dimethylpolysiloxanes - chemistry ; Equipment Design ; Fabrication ; Human tissues ; Humans ; Islets of Langerhans - cytology ; Male ; Membrane permeability ; Membranes, Artificial ; Microscopy, Electron, Scanning ; Models, Biological ; Morphogenesis ; Oxygen ; Oxygen - metabolism ; Oxygen availability ; Pancreas ; Pancreatic islets ; PDMS thickness ; Permeability ; Polydimethylsiloxane ; Rats, Sprague-Dawley ; Reproducibility of Results ; Secretion ; Silicone resins ; Spheroids ; Spheroids, Cellular ; Thickness ; Tissue culture ; Tissue engineering ; Tissue Engineering - instrumentation ; Tissue Engineering - methods ; Tissues</subject><ispartof>Acta biomaterialia, 2018-01, Vol.65, p.185-196</ispartof><rights>2017 Acta Materialia Inc.</rights><rights>Copyright © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier BV Jan 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-282131143a4f73e2a2bd0d7ec2662db91f851aa3f132b10924573049166573b23</citedby><cites>FETCH-LOGICAL-c427t-282131143a4f73e2a2bd0d7ec2662db91f851aa3f132b10924573049166573b23</cites><orcidid>0000-0003-0625-0660</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.actbio.2017.10.045$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29101017$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, GeonHui</creatorcontrib><creatorcontrib>Jun, Yesl</creatorcontrib><creatorcontrib>Jang, HeeYeong</creatorcontrib><creatorcontrib>Yoon, Junghyo</creatorcontrib><creatorcontrib>Lee, JaeSeo</creatorcontrib><creatorcontrib>Hong, MinHyung</creatorcontrib><creatorcontrib>Chung, Seok</creatorcontrib><creatorcontrib>Kim, Dong-Hwee</creatorcontrib><creatorcontrib>Lee, SangHoon</creatorcontrib><title>Enhanced oxygen permeability in membrane-bottomed concave microwells for the formation of pancreatic islet spheroids</title><title>Acta biomaterialia</title><addtitle>Acta Biomater</addtitle><description>[Display omitted]
Oxygen availability is a critical factor in regulating cell viability that ultimately contributes to the normal morphogenesis and functionality of human tissues. Among various cell culture platforms, construction of 3D multicellular spheroids based on microwell arrays has been extensively applied to reconstitute in vitro human tissue models due to its precise control of tissue culture conditions as well as simple fabrication processes. However, an adequate supply of oxygen into the spheroidal cellular aggregation still remains one of the main challenges to producing healthy in vitro spheroidal tissue models. Here, we present a novel design for controlling the oxygen distribution in concave microwell arrays. We show that oxygen permeability into the microwell is tightly regulated by varying the poly-dimethylsiloxane (PDMS) bottom thickness of the concave microwells. Moreover, we validate the enhanced performance of the engineered microwell arrays by culturing non-proliferated primary rat pancreatic islet spheroids on varying bottom thickness from 10 μm to 1050 μm. Morphological and functional analyses performed on the pancreatic islet spheroids grown for 14 days prove the long-term stability, enhanced viability, and increased hormone secretion under the sufficient oxygen delivery conditions. We expect our results could provide knowledge on oxygen distribution in 3-dimensional spheroidal cell structures and critical design concept for tissue engineering applications.
In this study, we present a noble design to control the oxygen distribution in concave microwell arrays for the formation of highly functional pancreatic islet spheroids by engineering the bottom of the microwells. Our new platform significantly enhanced oxygen permeability that turned out to improve cell viability and spheroidal functionality compared to the conventional thick-bottomed 3-D culture system. Therefore, we believe that this could be a promising medical biotechnology platform to further develop high-throughput tissue screening system as well as in vivo-mimicking customised 3-D tissue culture systems.</description><subject>3D spheroid</subject><subject>Animal models</subject><subject>Animals</subject><subject>Arrays</subject><subject>Cell culture</subject><subject>Cells, Cultured</subject><subject>Concave microwell</subject><subject>Design engineering</subject><subject>Dimethylpolysiloxanes - chemistry</subject><subject>Equipment Design</subject><subject>Fabrication</subject><subject>Human tissues</subject><subject>Humans</subject><subject>Islets of Langerhans - cytology</subject><subject>Male</subject><subject>Membrane permeability</subject><subject>Membranes, Artificial</subject><subject>Microscopy, Electron, Scanning</subject><subject>Models, Biological</subject><subject>Morphogenesis</subject><subject>Oxygen</subject><subject>Oxygen - metabolism</subject><subject>Oxygen availability</subject><subject>Pancreas</subject><subject>Pancreatic islets</subject><subject>PDMS thickness</subject><subject>Permeability</subject><subject>Polydimethylsiloxane</subject><subject>Rats, Sprague-Dawley</subject><subject>Reproducibility of Results</subject><subject>Secretion</subject><subject>Silicone resins</subject><subject>Spheroids</subject><subject>Spheroids, Cellular</subject><subject>Thickness</subject><subject>Tissue culture</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - instrumentation</subject><subject>Tissue Engineering - methods</subject><subject>Tissues</subject><issn>1742-7061</issn><issn>1878-7568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9UU1P3DAQtSpQobT_oEKWuPSSxeNk4-RSqVrRDwmpFzhbtjPpepXEwfZS9t930gUOPdSXGY_ee_PxGPsIYgUC6uvdyrhsfVhJAYpKK1Gt37BzaFRTqHXdnFCuKlkoUcMZe5fSToiyAdm8ZWeyJQminbN8M23N5LDj4enwCyc-YxzRWD_4fOB-4iOONpoJCxtyDiMBXZiceUQ-ehfDbxyGxPsQed7iEkeTfZh46PlMuhHp67hPA2ae5i3G4Lv0np32Zkj44TlesPuvN3eb78Xtz28_Nl9uC1dJlQvZSCgBqtJUvSpRGmk70Sl0sq5lZ1vomzUYU_ZQSguildValaJqoa4psbK8YJ-OunMMD3tMWY8-OZqY9gn7pKGtidXSmQh69Q90F_ZxoukI1fx9chGsjijaPKWIvZ6jH008aBB6cUXv9NEVvbiyVMkVol0-i-8tXfCV9GIDAT4fAUjXePQYdXIeF1t8RJd1F_z_O_wBT6Of4g</recordid><startdate>201801</startdate><enddate>201801</enddate><creator>Lee, GeonHui</creator><creator>Jun, Yesl</creator><creator>Jang, HeeYeong</creator><creator>Yoon, Junghyo</creator><creator>Lee, JaeSeo</creator><creator>Hong, MinHyung</creator><creator>Chung, Seok</creator><creator>Kim, Dong-Hwee</creator><creator>Lee, SangHoon</creator><general>Elsevier Ltd</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0625-0660</orcidid></search><sort><creationdate>201801</creationdate><title>Enhanced oxygen permeability in membrane-bottomed concave microwells for the formation of pancreatic islet spheroids</title><author>Lee, GeonHui ; Jun, Yesl ; Jang, HeeYeong ; Yoon, Junghyo ; Lee, JaeSeo ; Hong, MinHyung ; Chung, Seok ; Kim, Dong-Hwee ; Lee, SangHoon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-282131143a4f73e2a2bd0d7ec2662db91f851aa3f132b10924573049166573b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>3D spheroid</topic><topic>Animal models</topic><topic>Animals</topic><topic>Arrays</topic><topic>Cell culture</topic><topic>Cells, Cultured</topic><topic>Concave microwell</topic><topic>Design engineering</topic><topic>Dimethylpolysiloxanes - chemistry</topic><topic>Equipment Design</topic><topic>Fabrication</topic><topic>Human tissues</topic><topic>Humans</topic><topic>Islets of Langerhans - cytology</topic><topic>Male</topic><topic>Membrane permeability</topic><topic>Membranes, Artificial</topic><topic>Microscopy, Electron, Scanning</topic><topic>Models, Biological</topic><topic>Morphogenesis</topic><topic>Oxygen</topic><topic>Oxygen - metabolism</topic><topic>Oxygen availability</topic><topic>Pancreas</topic><topic>Pancreatic islets</topic><topic>PDMS thickness</topic><topic>Permeability</topic><topic>Polydimethylsiloxane</topic><topic>Rats, Sprague-Dawley</topic><topic>Reproducibility of Results</topic><topic>Secretion</topic><topic>Silicone resins</topic><topic>Spheroids</topic><topic>Spheroids, Cellular</topic><topic>Thickness</topic><topic>Tissue culture</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - instrumentation</topic><topic>Tissue Engineering - methods</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, GeonHui</creatorcontrib><creatorcontrib>Jun, Yesl</creatorcontrib><creatorcontrib>Jang, HeeYeong</creatorcontrib><creatorcontrib>Yoon, Junghyo</creatorcontrib><creatorcontrib>Lee, JaeSeo</creatorcontrib><creatorcontrib>Hong, MinHyung</creatorcontrib><creatorcontrib>Chung, Seok</creatorcontrib><creatorcontrib>Kim, Dong-Hwee</creatorcontrib><creatorcontrib>Lee, SangHoon</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biomaterialia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, GeonHui</au><au>Jun, Yesl</au><au>Jang, HeeYeong</au><au>Yoon, Junghyo</au><au>Lee, JaeSeo</au><au>Hong, MinHyung</au><au>Chung, Seok</au><au>Kim, Dong-Hwee</au><au>Lee, SangHoon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enhanced oxygen permeability in membrane-bottomed concave microwells for the formation of pancreatic islet spheroids</atitle><jtitle>Acta biomaterialia</jtitle><addtitle>Acta Biomater</addtitle><date>2018-01</date><risdate>2018</risdate><volume>65</volume><spage>185</spage><epage>196</epage><pages>185-196</pages><issn>1742-7061</issn><eissn>1878-7568</eissn><abstract>[Display omitted]
Oxygen availability is a critical factor in regulating cell viability that ultimately contributes to the normal morphogenesis and functionality of human tissues. Among various cell culture platforms, construction of 3D multicellular spheroids based on microwell arrays has been extensively applied to reconstitute in vitro human tissue models due to its precise control of tissue culture conditions as well as simple fabrication processes. However, an adequate supply of oxygen into the spheroidal cellular aggregation still remains one of the main challenges to producing healthy in vitro spheroidal tissue models. Here, we present a novel design for controlling the oxygen distribution in concave microwell arrays. We show that oxygen permeability into the microwell is tightly regulated by varying the poly-dimethylsiloxane (PDMS) bottom thickness of the concave microwells. Moreover, we validate the enhanced performance of the engineered microwell arrays by culturing non-proliferated primary rat pancreatic islet spheroids on varying bottom thickness from 10 μm to 1050 μm. Morphological and functional analyses performed on the pancreatic islet spheroids grown for 14 days prove the long-term stability, enhanced viability, and increased hormone secretion under the sufficient oxygen delivery conditions. We expect our results could provide knowledge on oxygen distribution in 3-dimensional spheroidal cell structures and critical design concept for tissue engineering applications.
In this study, we present a noble design to control the oxygen distribution in concave microwell arrays for the formation of highly functional pancreatic islet spheroids by engineering the bottom of the microwells. Our new platform significantly enhanced oxygen permeability that turned out to improve cell viability and spheroidal functionality compared to the conventional thick-bottomed 3-D culture system. Therefore, we believe that this could be a promising medical biotechnology platform to further develop high-throughput tissue screening system as well as in vivo-mimicking customised 3-D tissue culture systems.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29101017</pmid><doi>10.1016/j.actbio.2017.10.045</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-0625-0660</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1742-7061 |
| ispartof | Acta biomaterialia, 2018-01, Vol.65, p.185-196 |
| issn | 1742-7061 1878-7568 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1960929174 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 3D spheroid Animal models Animals Arrays Cell culture Cells, Cultured Concave microwell Design engineering Dimethylpolysiloxanes - chemistry Equipment Design Fabrication Human tissues Humans Islets of Langerhans - cytology Male Membrane permeability Membranes, Artificial Microscopy, Electron, Scanning Models, Biological Morphogenesis Oxygen Oxygen - metabolism Oxygen availability Pancreas Pancreatic islets PDMS thickness Permeability Polydimethylsiloxane Rats, Sprague-Dawley Reproducibility of Results Secretion Silicone resins Spheroids Spheroids, Cellular Thickness Tissue culture Tissue engineering Tissue Engineering - instrumentation Tissue Engineering - methods Tissues |
| title | Enhanced oxygen permeability in membrane-bottomed concave microwells for the formation of pancreatic islet spheroids |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T04%3A31%3A28IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enhanced%20oxygen%20permeability%20in%20membrane-bottomed%20concave%20microwells%20for%20the%20formation%20of%20pancreatic%20islet%20spheroids&rft.jtitle=Acta%20biomaterialia&rft.au=Lee,%20GeonHui&rft.date=2018-01&rft.volume=65&rft.spage=185&rft.epage=196&rft.pages=185-196&rft.issn=1742-7061&rft.eissn=1878-7568&rft_id=info:doi/10.1016/j.actbio.2017.10.045&rft_dat=%3Cproquest_cross%3E1988888822%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1988888822&rft_id=info:pmid/29101017&rft_els_id=S1742706117306736&rfr_iscdi=true |