Inhibition of 3,5,2′,4′-Tetrahydroxychalcone on Production of Uric Acid in Hypoxanthine-Induced Hyperuricemic Mice
The mechanism of 3,5,2′,4′-tetrahydroxychalcone on lowing urate level is still unknown. Here we investigated the effects of 3,5,2′,4′-tetrahydroxychalcone on urate levels, xanthine oxidase/xanthine dehydrogenase (XOD/XDH) activities in hypoxanthine-induced hyperuricemic mice, as well as the effects...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2018/01/01, Vol.41(1), pp.99-105 |
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| creator | Niu, Yanfen Zhou, Yuanfang Lin, Hua Gao, Li-Hui Xiong, Wenyong Zhu, Huajie Zou, Cheng-Gang Li, Ling |
| description | The mechanism of 3,5,2′,4′-tetrahydroxychalcone on lowing urate level is still unknown. Here we investigated the effects of 3,5,2′,4′-tetrahydroxychalcone on urate levels, xanthine oxidase/xanthine dehydrogenase (XOD/XDH) activities in hypoxanthine-induced hyperuricemic mice, as well as the effects of 3,5,2′,4′-tetrahydroxychalcone on the mRNA expression levels and content of phosphoribosyl pyrophosphate synthetase (PRPS), phosphoribosyl pyrophosphate amidotransferase (PRPPAT) and hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Our results demonstrated that 3,5,2′,4′-tetrahydroxychalcone (1.0, 2.0, and 4.0 mg/kg) reduced the uric acid levels in serum of the hyperuricemic mice in dose- and time-dependent manners. The activities of XOD/XDH in serum and liver were also significantly inhibited by 3,5,2′,4′-tetrahydroxychalcone; In addition, 3,5,2′,4′-tetrahydroxychalcone decreased the mRNA expression of HGPRT in brain and content of PRPS and PRPPAT in liver. These findings demonstrated that 3,5,2′,4′-tetrahydroxychalcone suppresses uric acid production by affecting the critical enzymes, XOD/XDH, PRPS, PRPPAT and HGPRT in purine nucleotide metabolism. |
| doi_str_mv | 10.1248/bpb.b17-00655 |
| format | Article |
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Here we investigated the effects of 3,5,2′,4′-tetrahydroxychalcone on urate levels, xanthine oxidase/xanthine dehydrogenase (XOD/XDH) activities in hypoxanthine-induced hyperuricemic mice, as well as the effects of 3,5,2′,4′-tetrahydroxychalcone on the mRNA expression levels and content of phosphoribosyl pyrophosphate synthetase (PRPS), phosphoribosyl pyrophosphate amidotransferase (PRPPAT) and hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Our results demonstrated that 3,5,2′,4′-tetrahydroxychalcone (1.0, 2.0, and 4.0 mg/kg) reduced the uric acid levels in serum of the hyperuricemic mice in dose- and time-dependent manners. The activities of XOD/XDH in serum and liver were also significantly inhibited by 3,5,2′,4′-tetrahydroxychalcone; In addition, 3,5,2′,4′-tetrahydroxychalcone decreased the mRNA expression of HGPRT in brain and content of PRPS and PRPPAT in liver. These findings demonstrated that 3,5,2′,4′-tetrahydroxychalcone suppresses uric acid production by affecting the critical enzymes, XOD/XDH, PRPS, PRPPAT and HGPRT in purine nucleotide metabolism.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b17-00655</identifier><identifier>PMID: 29093325</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>3,5,2′,4′-tetrahydroxychalcone ; Animals ; Chalcones - therapeutic use ; Disease Models, Animal ; Dose-Response Relationship, Drug ; hyperuricemia ; Hyperuricemia - chemically induced ; Hyperuricemia - drug therapy ; Hyperuricemia - metabolism ; Hypoxanthine - pharmacology ; Liver - drug effects ; Liver - metabolism ; Male ; Mice, Inbred Strains ; purine metabolism enzyme ; Purines - metabolism ; Uric Acid - blood ; Xanthine Dehydrogenase - genetics ; Xanthine Dehydrogenase - metabolism ; Xanthine Oxidase - genetics ; Xanthine Oxidase - metabolism</subject><ispartof>Biological and Pharmaceutical Bulletin, 2018/01/01, Vol.41(1), pp.99-105</ispartof><rights>2018 The Pharmaceutical Society of Japan</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c674t-3ee6491d6f89d363421e0dd23e8695a6469d683000a4bddced5295e42f54e5fc3</citedby><cites>FETCH-LOGICAL-c674t-3ee6491d6f89d363421e0dd23e8695a6469d683000a4bddced5295e42f54e5fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,4025,27925,27926,27927</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29093325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Niu, Yanfen</creatorcontrib><creatorcontrib>Zhou, Yuanfang</creatorcontrib><creatorcontrib>Lin, Hua</creatorcontrib><creatorcontrib>Gao, Li-Hui</creatorcontrib><creatorcontrib>Xiong, Wenyong</creatorcontrib><creatorcontrib>Zhu, Huajie</creatorcontrib><creatorcontrib>Zou, Cheng-Gang</creatorcontrib><creatorcontrib>Li, Ling</creatorcontrib><creatorcontrib>dUniversity of Chinese Academy of Sciences</creatorcontrib><creatorcontrib>Yunnan University</creatorcontrib><creatorcontrib>aState Laboratory for Conservation and Utilization of Bio-Resources in Yunnan</creatorcontrib><creatorcontrib>Chinese Academy of Sciences</creatorcontrib><creatorcontrib>HeiBei University</creatorcontrib><creatorcontrib>Kunming Medical University</creatorcontrib><creatorcontrib>Kunming Institute of Botany</creatorcontrib><creatorcontrib>bBiomedical Engineering Research Center</creatorcontrib><creatorcontrib>cState Key Laboratory of Phytochemistry and Plant Resources in West China</creatorcontrib><creatorcontrib>eThe Second Affiliated Hospital of Kunming Medical University</creatorcontrib><creatorcontrib>fCollege of Pharmacy</creatorcontrib><title>Inhibition of 3,5,2′,4′-Tetrahydroxychalcone on Production of Uric Acid in Hypoxanthine-Induced Hyperuricemic Mice</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The mechanism of 3,5,2′,4′-tetrahydroxychalcone on lowing urate level is still unknown. Here we investigated the effects of 3,5,2′,4′-tetrahydroxychalcone on urate levels, xanthine oxidase/xanthine dehydrogenase (XOD/XDH) activities in hypoxanthine-induced hyperuricemic mice, as well as the effects of 3,5,2′,4′-tetrahydroxychalcone on the mRNA expression levels and content of phosphoribosyl pyrophosphate synthetase (PRPS), phosphoribosyl pyrophosphate amidotransferase (PRPPAT) and hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Our results demonstrated that 3,5,2′,4′-tetrahydroxychalcone (1.0, 2.0, and 4.0 mg/kg) reduced the uric acid levels in serum of the hyperuricemic mice in dose- and time-dependent manners. The activities of XOD/XDH in serum and liver were also significantly inhibited by 3,5,2′,4′-tetrahydroxychalcone; In addition, 3,5,2′,4′-tetrahydroxychalcone decreased the mRNA expression of HGPRT in brain and content of PRPS and PRPPAT in liver. These findings demonstrated that 3,5,2′,4′-tetrahydroxychalcone suppresses uric acid production by affecting the critical enzymes, XOD/XDH, PRPS, PRPPAT and HGPRT in purine nucleotide metabolism.</description><subject>3,5,2′,4′-tetrahydroxychalcone</subject><subject>Animals</subject><subject>Chalcones - therapeutic use</subject><subject>Disease Models, Animal</subject><subject>Dose-Response Relationship, Drug</subject><subject>hyperuricemia</subject><subject>Hyperuricemia - chemically induced</subject><subject>Hyperuricemia - drug therapy</subject><subject>Hyperuricemia - metabolism</subject><subject>Hypoxanthine - pharmacology</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Mice, Inbred Strains</subject><subject>purine metabolism enzyme</subject><subject>Purines - metabolism</subject><subject>Uric Acid - blood</subject><subject>Xanthine Dehydrogenase - genetics</subject><subject>Xanthine Dehydrogenase - metabolism</subject><subject>Xanthine Oxidase - genetics</subject><subject>Xanthine Oxidase - metabolism</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc9uEzEQxi0EoqFw5Ir2yCFb_Hd3fawqaCMVwaE9W157ljja2MHerZobz8Qj8SRMmjaXGcvz8zcznwn5yOgF47L70u_6i561NaWNUq_IggnZ1ooz9ZosqGZd3TDVnZF3pWwopS3l4i0545pqIbhakIdVXIc-TCHFKg2VWKol__fn71JiqO9gyna99zk97t3aji5FqBD8mZOf3cub-xxcdemCr0Ksbva79GjjtA4R6lVEDPzhEvKMGGwR_Y75PXkz2LHAh-d8Tu6_fb27uqlvf1yvri5va9e0cqoFQCM1883QaS8aITkD6j0X0DVa2UY22jedwMWs7L3HXoprBZIPSoIanDgnn4-6u5x-z1Amsw3FwTjaCGkuhmmlBeeMdYjWR9TlVEqGwexy2Nq8N4yag9UGrTZotXmyGvlPz9JzvwV_ol-8ReD6CGA1ODumOKIpZpPmHHFn40rbhzQmwynrUFQyygxlEo9a44EqQZnS7UHp6qi0KZP9BadWNk_BjfA0mGSGHcJpwFMVfy4biOI_5PiqTg</recordid><startdate>20180101</startdate><enddate>20180101</enddate><creator>Niu, Yanfen</creator><creator>Zhou, Yuanfang</creator><creator>Lin, Hua</creator><creator>Gao, Li-Hui</creator><creator>Xiong, Wenyong</creator><creator>Zhu, Huajie</creator><creator>Zou, Cheng-Gang</creator><creator>Li, Ling</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20180101</creationdate><title>Inhibition of 3,5,2′,4′-Tetrahydroxychalcone on Production of Uric Acid in Hypoxanthine-Induced Hyperuricemic Mice</title><author>Niu, Yanfen ; 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Here we investigated the effects of 3,5,2′,4′-tetrahydroxychalcone on urate levels, xanthine oxidase/xanthine dehydrogenase (XOD/XDH) activities in hypoxanthine-induced hyperuricemic mice, as well as the effects of 3,5,2′,4′-tetrahydroxychalcone on the mRNA expression levels and content of phosphoribosyl pyrophosphate synthetase (PRPS), phosphoribosyl pyrophosphate amidotransferase (PRPPAT) and hypoxanthine-guanine phosphoribosyl transferase (HGPRT). Our results demonstrated that 3,5,2′,4′-tetrahydroxychalcone (1.0, 2.0, and 4.0 mg/kg) reduced the uric acid levels in serum of the hyperuricemic mice in dose- and time-dependent manners. The activities of XOD/XDH in serum and liver were also significantly inhibited by 3,5,2′,4′-tetrahydroxychalcone; In addition, 3,5,2′,4′-tetrahydroxychalcone decreased the mRNA expression of HGPRT in brain and content of PRPS and PRPPAT in liver. These findings demonstrated that 3,5,2′,4′-tetrahydroxychalcone suppresses uric acid production by affecting the critical enzymes, XOD/XDH, PRPS, PRPPAT and HGPRT in purine nucleotide metabolism.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>29093325</pmid><doi>10.1248/bpb.b17-00655</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3,5,2′,4′-tetrahydroxychalcone Animals Chalcones - therapeutic use Disease Models, Animal Dose-Response Relationship, Drug hyperuricemia Hyperuricemia - chemically induced Hyperuricemia - drug therapy Hyperuricemia - metabolism Hypoxanthine - pharmacology Liver - drug effects Liver - metabolism Male Mice, Inbred Strains purine metabolism enzyme Purines - metabolism Uric Acid - blood Xanthine Dehydrogenase - genetics Xanthine Dehydrogenase - metabolism Xanthine Oxidase - genetics Xanthine Oxidase - metabolism |
| title | Inhibition of 3,5,2′,4′-Tetrahydroxychalcone on Production of Uric Acid in Hypoxanthine-Induced Hyperuricemic Mice |
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