Biochemical and structural characterization of the hypoxanthine-guanine-xanthine phosphoribosyltransferase from Pyrococcus horikoshii

The 6-oxopurine phosphoribosyltransferase (HPRT, EC 2.4.2.8) from the hyperthermophile Pyrococcus horikoshii was expressed in Escherichia coli and purified. Steady-state kinetic studies indicated that the enzyme is able to use hypoxanthine, guanine and xanthine. The first two substrates showed simil...

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Veröffentlicht in:	Biochimica et biophysica acta 2008-06, Vol.1784 (6), p.953-960
Hauptverfasser:	de Souza Dantas, Deyse, Ramos dos Santos, Camila, Guimarães Pereira, Gonçalo Amarante, Medrano, Francisco Javier
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Archaeal Proteins - chemistry Archaeal Proteins - genetics Archaeal Proteins - metabolism Chromatography, Gel Circular Dichroism Dimerization Electrophoresis, Polyacrylamide Gel Escherichia coli Guanine - metabolism Guanosine Monophosphate - metabolism HGXPRT Hypoxanthine - metabolism Hypoxanthine-guanine-xanthine phosphoribosyltransferase Molecular Sequence Data Pentosyltransferases - chemistry Pentosyltransferases - genetics Pentosyltransferases - metabolism Pyrococcus horikoshii Pyrococcus horikoshii - enzymology Pyrococcus horikoshii - genetics Sequence Homology, Amino Acid Xanthine - metabolism
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