Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects
•In house validation shows inter-assay variation
Gespeichert in:
| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2018-06, Vol.106, p.114-124 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 124 |
|---|---|
| container_issue | |
| container_start_page | 114 |
| container_title | Cytokine (Philadelphia, Pa.) |
| container_volume | 106 |
| creator | Scholman, Rianne C. Giovannone, Barbara Hiddingh, Sanne Meerding, Jenny M. Malvar Fernandez, Beatriz van Dijk, Mariska E.A. Tempelman, Mariëlle J. Prakken, Berent J. de Jager, Wilco |
| description | •In house validation shows inter-assay variation |
| doi_str_mv | 10.1016/j.cyto.2017.10.021 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1958542552</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1043466617303368</els_id><sourcerecordid>1958542552</sourcerecordid><originalsourceid>FETCH-LOGICAL-c400t-5de86b46e57dfd9760b86079d3985722cc724087610b10f4551e14a23e4e96be3</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMorl9_wIPk6KXrJM1HA15k8QsWvCgeQ5tONcu2XZNW2H9vyq4ePc3MyzsvMw8hlwzmDJi6Wc3ddujnHJhOwhw4OyAnDIzKAHh-OPUiz4RSakZOY1wBgMm1PiYzbqAwTBcn5P2-adANtG9o2Q3e9eXHuE5dpH1HmeLU-eCSMvjug_q2HTukAdOMNd2EfkDfReo7-onlevjc0jhWq5QXz8lRU64jXuzrGXl7uH9dPGXLl8fnxd0ycwJgyGSNhaqEQqnrpjZaQVUo0KbOTSE1585pLqDQikHFoBFSMmSi5DkKNKrC_Ixc73LTMV8jxsG2Pjpcpx-wH6NlRhZScCl5svKd1YU-xoCN3QTflmFrGdgJqF3ZCaidgE5aApqWrvb5Y9Vi_bfySzAZbncGTF9-eww2Oo-dw9qHBMLWvf8v_wf78YaM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1958542552</pqid></control><display><type>article</type><title>Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Scholman, Rianne C. ; Giovannone, Barbara ; Hiddingh, Sanne ; Meerding, Jenny M. ; Malvar Fernandez, Beatriz ; van Dijk, Mariska E.A. ; Tempelman, Mariëlle J. ; Prakken, Berent J. ; de Jager, Wilco</creator><creatorcontrib>Scholman, Rianne C. ; Giovannone, Barbara ; Hiddingh, Sanne ; Meerding, Jenny M. ; Malvar Fernandez, Beatriz ; van Dijk, Mariska E.A. ; Tempelman, Mariëlle J. ; Prakken, Berent J. ; de Jager, Wilco</creatorcontrib><description>•In house validation shows inter-assay variation <10%, and average inter-assay variation of 12.2%•Immune profiles remain stable after multiple freeze–thaw cycles.•Only 19 out of 162 soluble proteins have similar expression in serum, EDTA plasma and sodium heparin plasma.
Diagnosis of complex disease and response to treatment is often associated with multiple indicators, both clinical and laboratorial. With the use of biomarkers, various mechanisms have been unraveled which can lead to better and faster diagnosis, predicting and monitoring of response to treatment and new drug development. With the introduction of multiplex technology for immunoassays and the growing awareness of the role of immune-monitoring during new therapeutic interventions it is now possible to test large numbers of soluble mediators in small sample volumes. However, standardization of sample collection and laboratory assessments remains suboptimal.
We developed a multiplex immunoassay for detection of 162 immune related proteins in human serum and plasma. The assay was split in panels depending on natural occurring concentrations with a maximum of 60 proteins. The aim of this study was to evaluate precision, accuracy, reproducibility and stability of proteins when repeated freeze–thaw cycles are performed of this in-house developed panel, as well as assessing the protein signature in plasma and serum using various anticoagulants.
Intra-assay variance of each mediator was <10%. Inter-assay variance ranged between 1.6 and 37% with an average of 12.2%. Recoveries were similar for all mediators (mean 99.8 ± 2.6%) with a range between 89–107%. Next we measured all mediators in serum, EDTA plasma and sodium heparin plasma of 43 healthy control donors. Of these markers only 19 showed similar expression profiles in the 3 different matrixes. Only 5 mediators were effected by multiple freeze-thawing cycles. Principal component analysis revealed different coagulants cluster separately and that sodium heparin shows the most consistent profile.</description><identifier>ISSN: 1043-4666</identifier><identifier>EISSN: 1096-0023</identifier><identifier>DOI: 10.1016/j.cyto.2017.10.021</identifier><identifier>PMID: 29089178</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Adult ; Anticoagulants ; Anticoagulants - pharmacology ; Cytokine ; Edetic Acid - pharmacology ; Female ; Freezing ; Healthy Volunteers ; Heparin - pharmacology ; Humans ; Immunoassay ; Immunoproteins - metabolism ; Limit of Detection ; Luminex ; Male ; Middle Aged ; Plasma ; Protein Stability ; Reference Standards ; Reproducibility of Results ; Serum ; Validation</subject><ispartof>Cytokine (Philadelphia, Pa.), 2018-06, Vol.106, p.114-124</ispartof><rights>2017 The Authors</rights><rights>Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-5de86b46e57dfd9760b86079d3985722cc724087610b10f4551e14a23e4e96be3</citedby><cites>FETCH-LOGICAL-c400t-5de86b46e57dfd9760b86079d3985722cc724087610b10f4551e14a23e4e96be3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1043466617303368$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29089178$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Scholman, Rianne C.</creatorcontrib><creatorcontrib>Giovannone, Barbara</creatorcontrib><creatorcontrib>Hiddingh, Sanne</creatorcontrib><creatorcontrib>Meerding, Jenny M.</creatorcontrib><creatorcontrib>Malvar Fernandez, Beatriz</creatorcontrib><creatorcontrib>van Dijk, Mariska E.A.</creatorcontrib><creatorcontrib>Tempelman, Mariëlle J.</creatorcontrib><creatorcontrib>Prakken, Berent J.</creatorcontrib><creatorcontrib>de Jager, Wilco</creatorcontrib><title>Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects</title><title>Cytokine (Philadelphia, Pa.)</title><addtitle>Cytokine</addtitle><description>•In house validation shows inter-assay variation <10%, and average inter-assay variation of 12.2%•Immune profiles remain stable after multiple freeze–thaw cycles.•Only 19 out of 162 soluble proteins have similar expression in serum, EDTA plasma and sodium heparin plasma.
Diagnosis of complex disease and response to treatment is often associated with multiple indicators, both clinical and laboratorial. With the use of biomarkers, various mechanisms have been unraveled which can lead to better and faster diagnosis, predicting and monitoring of response to treatment and new drug development. With the introduction of multiplex technology for immunoassays and the growing awareness of the role of immune-monitoring during new therapeutic interventions it is now possible to test large numbers of soluble mediators in small sample volumes. However, standardization of sample collection and laboratory assessments remains suboptimal.
We developed a multiplex immunoassay for detection of 162 immune related proteins in human serum and plasma. The assay was split in panels depending on natural occurring concentrations with a maximum of 60 proteins. The aim of this study was to evaluate precision, accuracy, reproducibility and stability of proteins when repeated freeze–thaw cycles are performed of this in-house developed panel, as well as assessing the protein signature in plasma and serum using various anticoagulants.
Intra-assay variance of each mediator was <10%. Inter-assay variance ranged between 1.6 and 37% with an average of 12.2%. Recoveries were similar for all mediators (mean 99.8 ± 2.6%) with a range between 89–107%. Next we measured all mediators in serum, EDTA plasma and sodium heparin plasma of 43 healthy control donors. Of these markers only 19 showed similar expression profiles in the 3 different matrixes. Only 5 mediators were effected by multiple freeze-thawing cycles. Principal component analysis revealed different coagulants cluster separately and that sodium heparin shows the most consistent profile.</description><subject>Adult</subject><subject>Anticoagulants</subject><subject>Anticoagulants - pharmacology</subject><subject>Cytokine</subject><subject>Edetic Acid - pharmacology</subject><subject>Female</subject><subject>Freezing</subject><subject>Healthy Volunteers</subject><subject>Heparin - pharmacology</subject><subject>Humans</subject><subject>Immunoassay</subject><subject>Immunoproteins - metabolism</subject><subject>Limit of Detection</subject><subject>Luminex</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Plasma</subject><subject>Protein Stability</subject><subject>Reference Standards</subject><subject>Reproducibility of Results</subject><subject>Serum</subject><subject>Validation</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMorl9_wIPk6KXrJM1HA15k8QsWvCgeQ5tONcu2XZNW2H9vyq4ePc3MyzsvMw8hlwzmDJi6Wc3ddujnHJhOwhw4OyAnDIzKAHh-OPUiz4RSakZOY1wBgMm1PiYzbqAwTBcn5P2-adANtG9o2Q3e9eXHuE5dpH1HmeLU-eCSMvjug_q2HTukAdOMNd2EfkDfReo7-onlevjc0jhWq5QXz8lRU64jXuzrGXl7uH9dPGXLl8fnxd0ycwJgyGSNhaqEQqnrpjZaQVUo0KbOTSE1585pLqDQikHFoBFSMmSi5DkKNKrC_Ixc73LTMV8jxsG2Pjpcpx-wH6NlRhZScCl5svKd1YU-xoCN3QTflmFrGdgJqF3ZCaidgE5aApqWrvb5Y9Vi_bfySzAZbncGTF9-eww2Oo-dw9qHBMLWvf8v_wf78YaM</recordid><startdate>201806</startdate><enddate>201806</enddate><creator>Scholman, Rianne C.</creator><creator>Giovannone, Barbara</creator><creator>Hiddingh, Sanne</creator><creator>Meerding, Jenny M.</creator><creator>Malvar Fernandez, Beatriz</creator><creator>van Dijk, Mariska E.A.</creator><creator>Tempelman, Mariëlle J.</creator><creator>Prakken, Berent J.</creator><creator>de Jager, Wilco</creator><general>Elsevier Ltd</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201806</creationdate><title>Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects</title><author>Scholman, Rianne C. ; Giovannone, Barbara ; Hiddingh, Sanne ; Meerding, Jenny M. ; Malvar Fernandez, Beatriz ; van Dijk, Mariska E.A. ; Tempelman, Mariëlle J. ; Prakken, Berent J. ; de Jager, Wilco</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-5de86b46e57dfd9760b86079d3985722cc724087610b10f4551e14a23e4e96be3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Adult</topic><topic>Anticoagulants</topic><topic>Anticoagulants - pharmacology</topic><topic>Cytokine</topic><topic>Edetic Acid - pharmacology</topic><topic>Female</topic><topic>Freezing</topic><topic>Healthy Volunteers</topic><topic>Heparin - pharmacology</topic><topic>Humans</topic><topic>Immunoassay</topic><topic>Immunoproteins - metabolism</topic><topic>Limit of Detection</topic><topic>Luminex</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Plasma</topic><topic>Protein Stability</topic><topic>Reference Standards</topic><topic>Reproducibility of Results</topic><topic>Serum</topic><topic>Validation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Scholman, Rianne C.</creatorcontrib><creatorcontrib>Giovannone, Barbara</creatorcontrib><creatorcontrib>Hiddingh, Sanne</creatorcontrib><creatorcontrib>Meerding, Jenny M.</creatorcontrib><creatorcontrib>Malvar Fernandez, Beatriz</creatorcontrib><creatorcontrib>van Dijk, Mariska E.A.</creatorcontrib><creatorcontrib>Tempelman, Mariëlle J.</creatorcontrib><creatorcontrib>Prakken, Berent J.</creatorcontrib><creatorcontrib>de Jager, Wilco</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Scholman, Rianne C.</au><au>Giovannone, Barbara</au><au>Hiddingh, Sanne</au><au>Meerding, Jenny M.</au><au>Malvar Fernandez, Beatriz</au><au>van Dijk, Mariska E.A.</au><au>Tempelman, Mariëlle J.</au><au>Prakken, Berent J.</au><au>de Jager, Wilco</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>2018-06</date><risdate>2018</risdate><volume>106</volume><spage>114</spage><epage>124</epage><pages>114-124</pages><issn>1043-4666</issn><eissn>1096-0023</eissn><abstract>•In house validation shows inter-assay variation <10%, and average inter-assay variation of 12.2%•Immune profiles remain stable after multiple freeze–thaw cycles.•Only 19 out of 162 soluble proteins have similar expression in serum, EDTA plasma and sodium heparin plasma.
Diagnosis of complex disease and response to treatment is often associated with multiple indicators, both clinical and laboratorial. With the use of biomarkers, various mechanisms have been unraveled which can lead to better and faster diagnosis, predicting and monitoring of response to treatment and new drug development. With the introduction of multiplex technology for immunoassays and the growing awareness of the role of immune-monitoring during new therapeutic interventions it is now possible to test large numbers of soluble mediators in small sample volumes. However, standardization of sample collection and laboratory assessments remains suboptimal.
We developed a multiplex immunoassay for detection of 162 immune related proteins in human serum and plasma. The assay was split in panels depending on natural occurring concentrations with a maximum of 60 proteins. The aim of this study was to evaluate precision, accuracy, reproducibility and stability of proteins when repeated freeze–thaw cycles are performed of this in-house developed panel, as well as assessing the protein signature in plasma and serum using various anticoagulants.
Intra-assay variance of each mediator was <10%. Inter-assay variance ranged between 1.6 and 37% with an average of 12.2%. Recoveries were similar for all mediators (mean 99.8 ± 2.6%) with a range between 89–107%. Next we measured all mediators in serum, EDTA plasma and sodium heparin plasma of 43 healthy control donors. Of these markers only 19 showed similar expression profiles in the 3 different matrixes. Only 5 mediators were effected by multiple freeze-thawing cycles. Principal component analysis revealed different coagulants cluster separately and that sodium heparin shows the most consistent profile.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>29089178</pmid><doi>10.1016/j.cyto.2017.10.021</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1043-4666 |
| ispartof | Cytokine (Philadelphia, Pa.), 2018-06, Vol.106, p.114-124 |
| issn | 1043-4666 1096-0023 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1958542552 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Adult Anticoagulants Anticoagulants - pharmacology Cytokine Edetic Acid - pharmacology Female Freezing Healthy Volunteers Heparin - pharmacology Humans Immunoassay Immunoproteins - metabolism Limit of Detection Luminex Male Middle Aged Plasma Protein Stability Reference Standards Reproducibility of Results Serum Validation |
| title | Effect of anticoagulants on 162 circulating immune related proteins in healthy subjects |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-01T17%3A02%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20anticoagulants%20on%20162%20circulating%20immune%20related%20proteins%20in%20healthy%20subjects&rft.jtitle=Cytokine%20(Philadelphia,%20Pa.)&rft.au=Scholman,%20Rianne%20C.&rft.date=2018-06&rft.volume=106&rft.spage=114&rft.epage=124&rft.pages=114-124&rft.issn=1043-4666&rft.eissn=1096-0023&rft_id=info:doi/10.1016/j.cyto.2017.10.021&rft_dat=%3Cproquest_cross%3E1958542552%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1958542552&rft_id=info:pmid/29089178&rft_els_id=S1043466617303368&rfr_iscdi=true |