Development of a bicistronic expression system in the branchiopod crustacean Daphnia magna

Summary The viral 2A peptides have recently been used for bicistronic expression in various organisms. In this system, a single mRNA that codes for two proteins flanking the 2A peptide can be translated simultaneously into each protein by ribosomal skipping at this peptide sequence. Here, we tested...

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Veröffentlicht in:	Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2017-12, Vol.55 (12), p.n/a
Hauptverfasser:	Kumagai, Hitoshi, Matsuura, Tomoaki, Kato, Yasuhiko, Watanabe, Hajime
Format:	Artikel
Sprache:	eng
Schlagworte:	2A peptide bicistronic expression Biological evolution Crustaceans Cytoplasm Daphnia magna Developmental biology Eggs evo‐devo Fluorescence Gastrulation Gene expression Green fluorescent protein Histone H2B Insects Localization membrane localization mRNA Myristoylation nuclear localization Open reading frames Peptides Proteins Ribonucleic acid RNA Viruses
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