Co-expression of Tbx6 and Sox2 identifies a novel transient neuromesoderm progenitor cell state
Elongation of the body axis is a key aspect of body plan development. Bipotential neuromesoderm progenitors (NMPs) ensure axial growth of embryos by contributing both to the spinal cord and mesoderm. The current model for the mechanism controlling NMP deployment invokes Tbx6, a T-box factor, to driv...
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| Veröffentlicht in: | Development (Cambridge) 2017-12, Vol.144 (24), p.4522-4529 |
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| container_issue | 24 |
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| container_title | Development (Cambridge) |
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| creator | Javali, Alok Misra, Aritra Leonavicius, Karolis Acharyya, Debalina Vyas, Bhakti Sambasivan, Ramkumar |
| description | Elongation of the body axis is a key aspect of body plan development. Bipotential neuromesoderm progenitors (NMPs) ensure axial growth of embryos by contributing both to the spinal cord and mesoderm. The current model for the mechanism controlling NMP deployment invokes Tbx6, a T-box factor, to drive mesoderm differentiation of NMPs. Here, we identify a new population of Tbx6
cells in a subdomain of the NMP niche in mouse embryos. Based on co-expression of a progenitor marker, Sox2, we identify this population as representing a transient cell state in the mesoderm-fated NMP lineage. Genetic lineage tracing confirms the presence of the
NMP cell state. Furthermore, we report a novel aspect of the documented
mutant phenotype, namely an increase from two to four ectopic neural tubes, corresponding to the switch in NMP niche, thus highlighting the importance of
function in NMP fate decision. This study emphasizes the function of Tbx6 as a bistable switch that turns mesoderm fate 'on' and progenitor state 'off', and thus has implications for the molecular mechanism driving NMP fate choice. |
| doi_str_mv | 10.1242/dev.153262 |
| format | Article |
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NMP cell state. Furthermore, we report a novel aspect of the documented
mutant phenotype, namely an increase from two to four ectopic neural tubes, corresponding to the switch in NMP niche, thus highlighting the importance of
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NMP cell state. Furthermore, we report a novel aspect of the documented
mutant phenotype, namely an increase from two to four ectopic neural tubes, corresponding to the switch in NMP niche, thus highlighting the importance of
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cells in a subdomain of the NMP niche in mouse embryos. Based on co-expression of a progenitor marker, Sox2, we identify this population as representing a transient cell state in the mesoderm-fated NMP lineage. Genetic lineage tracing confirms the presence of the
NMP cell state. Furthermore, we report a novel aspect of the documented
mutant phenotype, namely an increase from two to four ectopic neural tubes, corresponding to the switch in NMP niche, thus highlighting the importance of
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| fulltext | fulltext |
| identifier | ISSN: 0950-1991 |
| ispartof | Development (Cambridge), 2017-12, Vol.144 (24), p.4522-4529 |
| issn | 0950-1991 1477-9129 |
| language | eng |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Company of Biologists |
| subjects | Embryos Mesoderm Neural stem cells Progenitor cells Spinal cord |
| title | Co-expression of Tbx6 and Sox2 identifies a novel transient neuromesoderm progenitor cell state |
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