Curcumin inhibits the proliferation and induces apoptosis in HT-29 cell lines through a reactive oxygen species (ROS)-dependent mechanism
Curcumin, a natural pigment extracted from Curcuma longa, has anti-carcinogenic activities in many cancer cell lines. The molecular mechanism of apoptosis induced by curcumin are still unknown. In the current study, we investigated the roles of reactive oxygen species in curcumin stimulated apoptosi...
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| Veröffentlicht in: | Pakistan journal of pharmaceutical sciences 2017-09, Vol.30 (5), p.1671-1677 |
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| container_title | Pakistan journal of pharmaceutical sciences |
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| creator | Wang, Jinbo Qi, Lili Mei, Lehe Wu, Zhige |
| description | Curcumin, a natural pigment extracted from Curcuma longa, has anti-carcinogenic activities in many cancer cell lines. The molecular mechanism of apoptosis induced by curcumin are still unknown. In the current study, we investigated the roles of reactive oxygen species in curcumin stimulated apoptosis in HT-29 cells. Curcumin significantly reduced cell viability, induced apoptosis, activated caspase-3 activity and stimulated concentration-dependent release of ROS. Inhibition of ROS generation by scavengers suppressed apoptosis and Bcl-2 expression induced by curcumin, indicating the critical roles of ROS in the apoptotic process. However, caspase-3 inhibitor (z-VAD-FMK) couldn't completely inhibit the curcumin induced apoptosis, indicating ROS mediated apoptosis may be caspase-independent. Together, our findings showed that ROS played significant roles in the apoptosis induced by curcumin in HT-29 cells. |
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The molecular mechanism of apoptosis induced by curcumin are still unknown. In the current study, we investigated the roles of reactive oxygen species in curcumin stimulated apoptosis in HT-29 cells. Curcumin significantly reduced cell viability, induced apoptosis, activated caspase-3 activity and stimulated concentration-dependent release of ROS. Inhibition of ROS generation by scavengers suppressed apoptosis and Bcl-2 expression induced by curcumin, indicating the critical roles of ROS in the apoptotic process. However, caspase-3 inhibitor (z-VAD-FMK) couldn't completely inhibit the curcumin induced apoptosis, indicating ROS mediated apoptosis may be caspase-independent. Together, our findings showed that ROS played significant roles in the apoptosis induced by curcumin in HT-29 cells.</description><identifier>ISSN: 1011-601X</identifier><identifier>PMID: 29084689</identifier><language>eng</language><publisher>Pakistan: Pakistan Journal of Pharmaceutical Sciences</publisher><subject>Antineoplastic Agents, Phytogenic - pharmacology ; Apoptosis - drug effects ; Cancer research ; Cancer treatment ; Caspase 3 - metabolism ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - metabolism ; Colonic Neoplasms - pathology ; Curcumin ; Curcumin - pharmacology ; Health aspects ; HT29 Cells ; Humans ; Oxidative Stress - drug effects ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Signal Transduction - drug effects</subject><ispartof>Pakistan journal of pharmaceutical sciences, 2017-09, Vol.30 (5), p.1671-1677</ispartof><rights>COPYRIGHT 2017 Pakistan Journal of Pharmaceutical Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29084689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jinbo</creatorcontrib><creatorcontrib>Qi, Lili</creatorcontrib><creatorcontrib>Mei, Lehe</creatorcontrib><creatorcontrib>Wu, Zhige</creatorcontrib><title>Curcumin inhibits the proliferation and induces apoptosis in HT-29 cell lines through a reactive oxygen species (ROS)-dependent mechanism</title><title>Pakistan journal of pharmaceutical sciences</title><addtitle>Pak J Pharm Sci</addtitle><description>Curcumin, a natural pigment extracted from Curcuma longa, has anti-carcinogenic activities in many cancer cell lines. The molecular mechanism of apoptosis induced by curcumin are still unknown. In the current study, we investigated the roles of reactive oxygen species in curcumin stimulated apoptosis in HT-29 cells. Curcumin significantly reduced cell viability, induced apoptosis, activated caspase-3 activity and stimulated concentration-dependent release of ROS. Inhibition of ROS generation by scavengers suppressed apoptosis and Bcl-2 expression induced by curcumin, indicating the critical roles of ROS in the apoptotic process. However, caspase-3 inhibitor (z-VAD-FMK) couldn't completely inhibit the curcumin induced apoptosis, indicating ROS mediated apoptosis may be caspase-independent. Together, our findings showed that ROS played significant roles in the apoptosis induced by curcumin in HT-29 cells.</description><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>Apoptosis - drug effects</subject><subject>Cancer research</subject><subject>Cancer treatment</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - metabolism</subject><subject>Colonic Neoplasms - pathology</subject><subject>Curcumin</subject><subject>Curcumin - pharmacology</subject><subject>Health aspects</subject><subject>HT29 Cells</subject><subject>Humans</subject><subject>Oxidative Stress - drug effects</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Signal Transduction - drug effects</subject><issn>1011-601X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkU1LxDAQhntQ_P4LEvCyHipJm8TkKIu6grDgB3graTLZjbRJbVJxf4L_2izqQZA5DLzzvMO8zE5xQDAhJcfkZb84jPEVY06llHvFfiWxoFzIg-JzPo166p1Hzq9d61JEaQ1oGEPnLIwqueCR8iaPzaQhIjWEIYXoYlbQ4qmsJNLQdahzHrbeMUyrNVJoBKWTewcUPjYr8CgOoF0mZg_Lx_PSwADegE-oB71W3sX-uNi1qotw8tOPiueb66f5orxf3t7Nr-7LVXUpUslpy2uCcavaKsewleGC1kCEbTGhlTQCC2YupTK0bi2zltKq1lwxJjADReqjYva9N2d8myCmpndxG0F5CFNsiGSC1YzXLKNn3-hKddA4b0Mald7izRUjuJKcUJqpi3-oXAZ6p4MH67L-x3D6c8HU9mCaYXS9GjfN71fqLxGOh1s</recordid><startdate>201709</startdate><enddate>201709</enddate><creator>Wang, Jinbo</creator><creator>Qi, Lili</creator><creator>Mei, Lehe</creator><creator>Wu, Zhige</creator><general>Pakistan Journal of Pharmaceutical Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201709</creationdate><title>Curcumin inhibits the proliferation and induces apoptosis in HT-29 cell lines through a reactive oxygen species (ROS)-dependent mechanism</title><author>Wang, Jinbo ; Qi, Lili ; Mei, Lehe ; Wu, Zhige</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g278t-64b63100bab2290f2d6843e18fb01429d8085d79ad43bf5ff4423c6a55805ea13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>Apoptosis - drug effects</topic><topic>Cancer research</topic><topic>Cancer treatment</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - metabolism</topic><topic>Colonic Neoplasms - pathology</topic><topic>Curcumin</topic><topic>Curcumin - pharmacology</topic><topic>Health aspects</topic><topic>HT29 Cells</topic><topic>Humans</topic><topic>Oxidative Stress - drug effects</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Reactive oxygen species</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Signal Transduction - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jinbo</creatorcontrib><creatorcontrib>Qi, Lili</creatorcontrib><creatorcontrib>Mei, Lehe</creatorcontrib><creatorcontrib>Wu, Zhige</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jinbo</au><au>Qi, Lili</au><au>Mei, Lehe</au><au>Wu, Zhige</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curcumin inhibits the proliferation and induces apoptosis in HT-29 cell lines through a reactive oxygen species (ROS)-dependent mechanism</atitle><jtitle>Pakistan journal of pharmaceutical sciences</jtitle><addtitle>Pak J Pharm Sci</addtitle><date>2017-09</date><risdate>2017</risdate><volume>30</volume><issue>5</issue><spage>1671</spage><epage>1677</epage><pages>1671-1677</pages><issn>1011-601X</issn><abstract>Curcumin, a natural pigment extracted from Curcuma longa, has anti-carcinogenic activities in many cancer cell lines. The molecular mechanism of apoptosis induced by curcumin are still unknown. In the current study, we investigated the roles of reactive oxygen species in curcumin stimulated apoptosis in HT-29 cells. Curcumin significantly reduced cell viability, induced apoptosis, activated caspase-3 activity and stimulated concentration-dependent release of ROS. Inhibition of ROS generation by scavengers suppressed apoptosis and Bcl-2 expression induced by curcumin, indicating the critical roles of ROS in the apoptotic process. However, caspase-3 inhibitor (z-VAD-FMK) couldn't completely inhibit the curcumin induced apoptosis, indicating ROS mediated apoptosis may be caspase-independent. Together, our findings showed that ROS played significant roles in the apoptosis induced by curcumin in HT-29 cells.</abstract><cop>Pakistan</cop><pub>Pakistan Journal of Pharmaceutical Sciences</pub><pmid>29084689</pmid><tpages>7</tpages></addata></record> |
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| ispartof | Pakistan journal of pharmaceutical sciences, 2017-09, Vol.30 (5), p.1671-1677 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Antineoplastic Agents, Phytogenic - pharmacology Apoptosis - drug effects Cancer research Cancer treatment Caspase 3 - metabolism Cell Proliferation - drug effects Cell Survival - drug effects Colonic Neoplasms - drug therapy Colonic Neoplasms - metabolism Colonic Neoplasms - pathology Curcumin Curcumin - pharmacology Health aspects HT29 Cells Humans Oxidative Stress - drug effects Proto-Oncogene Proteins c-bcl-2 - metabolism Reactive oxygen species Reactive Oxygen Species - metabolism Signal Transduction - drug effects |
| title | Curcumin inhibits the proliferation and induces apoptosis in HT-29 cell lines through a reactive oxygen species (ROS)-dependent mechanism |
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