Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY
Oneida Lake is a shallow, eutrophic lake with a well-established cyanobacterial population with reported toxic blooms containing hepatotoxic microcystins (MC). Peak bloom events from the summers of 2002 and 2003 were analyzed to determine the principal cyanobacterial genera containing microcystin sy...
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| Veröffentlicht in: | Harmful algae 2008-08, Vol.7 (5), p.671-681 |
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| creator | Hotto, Amber M. Satchwell, Mike F. Berry, Dianna L. Gobler, Christopher J. Boyer, Gregory L. |
| description | Oneida Lake is a shallow, eutrophic lake with a well-established cyanobacterial population with reported toxic blooms containing hepatotoxic microcystins (MC). Peak bloom events from the summers of 2002 and 2003 were analyzed to determine the principal cyanobacterial genera containing microcystin synthetase (
mcy) genes. Sequence analysis of a partial
mcyA amplicon targeting
Microcystis,
Anabaena and
Planktothrix sp. indicated that
Microcystis sp. was the dominant
mcy genotype. This
Microcystis clade was split into two distinct sub-clades. Bloom events contained members of both sub-clades with the higher MC concentrations found when both sub-clades were present in near equal proportions. The proportion of
Microcystis containing the
mcyD gene ranged from 0 to 37% of the total
Microcystis population as determined by quantitative PCR (qPCR). The total concentration of
Microcystis containing
mcyD genes was linearly related to the concentration of MCs (
r
2
=
0.63). The relationship between
mcy genotype and physiochemical variables was examined to determine the factor(s) controlling the periodicity in MC production in Oneida Lake. Multivariate statistical analyses, used to correlate the continuous-response variables, revealed a strong relationship between chlorophyll
a, MCs and total
Microcystis. |
| doi_str_mv | 10.1016/j.hal.2008.02.001 |
| format | Article |
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mcy) genes. Sequence analysis of a partial
mcyA amplicon targeting
Microcystis,
Anabaena and
Planktothrix sp. indicated that
Microcystis sp. was the dominant
mcy genotype. This
Microcystis clade was split into two distinct sub-clades. Bloom events contained members of both sub-clades with the higher MC concentrations found when both sub-clades were present in near equal proportions. The proportion of
Microcystis containing the
mcyD gene ranged from 0 to 37% of the total
Microcystis population as determined by quantitative PCR (qPCR). The total concentration of
Microcystis containing
mcyD genes was linearly related to the concentration of MCs (
r
2
=
0.63). The relationship between
mcy genotype and physiochemical variables was examined to determine the factor(s) controlling the periodicity in MC production in Oneida Lake. Multivariate statistical analyses, used to correlate the continuous-response variables, revealed a strong relationship between chlorophyll
a, MCs and total
Microcystis.</description><identifier>ISSN: 1568-9883</identifier><identifier>EISSN: 1878-1470</identifier><identifier>DOI: 10.1016/j.hal.2008.02.001</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Algae ; Anabaena ; Animal and plant ecology ; Animal, plant and microbial ecology ; Autoecology ; Biological and medical sciences ; Cyanobacteria ; Freshwater ; Fundamental and applied biological sciences. Psychology ; mcy genes ; Microcystin ; Microcystis ; Oneida Lake ; Planktothrix ; Plant cytology, morphology, systematics, chorology and evolution ; Plants and fungi ; qPCR ; Thallophyta</subject><ispartof>Harmful algae, 2008-08, Vol.7 (5), p.671-681</ispartof><rights>2008 Elsevier B.V.</rights><rights>2008 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c358t-87e08ebb49cf89f849abf40ee0fe8f3a2209c631d8f125971e9509ca33a86e1a3</citedby><cites>FETCH-LOGICAL-c358t-87e08ebb49cf89f849abf40ee0fe8f3a2209c631d8f125971e9509ca33a86e1a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1568988308000176$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20431721$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Hotto, Amber M.</creatorcontrib><creatorcontrib>Satchwell, Mike F.</creatorcontrib><creatorcontrib>Berry, Dianna L.</creatorcontrib><creatorcontrib>Gobler, Christopher J.</creatorcontrib><creatorcontrib>Boyer, Gregory L.</creatorcontrib><title>Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY</title><title>Harmful algae</title><description>Oneida Lake is a shallow, eutrophic lake with a well-established cyanobacterial population with reported toxic blooms containing hepatotoxic microcystins (MC). Peak bloom events from the summers of 2002 and 2003 were analyzed to determine the principal cyanobacterial genera containing microcystin synthetase (
mcy) genes. Sequence analysis of a partial
mcyA amplicon targeting
Microcystis,
Anabaena and
Planktothrix sp. indicated that
Microcystis sp. was the dominant
mcy genotype. This
Microcystis clade was split into two distinct sub-clades. Bloom events contained members of both sub-clades with the higher MC concentrations found when both sub-clades were present in near equal proportions. The proportion of
Microcystis containing the
mcyD gene ranged from 0 to 37% of the total
Microcystis population as determined by quantitative PCR (qPCR). The total concentration of
Microcystis containing
mcyD genes was linearly related to the concentration of MCs (
r
2
=
0.63). The relationship between
mcy genotype and physiochemical variables was examined to determine the factor(s) controlling the periodicity in MC production in Oneida Lake. Multivariate statistical analyses, used to correlate the continuous-response variables, revealed a strong relationship between chlorophyll
a, MCs and total
Microcystis.</description><subject>Algae</subject><subject>Anabaena</subject><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Autoecology</subject><subject>Biological and medical sciences</subject><subject>Cyanobacteria</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>mcy genes</subject><subject>Microcystin</subject><subject>Microcystis</subject><subject>Oneida Lake</subject><subject>Planktothrix</subject><subject>Plant cytology, morphology, systematics, chorology and evolution</subject><subject>Plants and fungi</subject><subject>qPCR</subject><subject>Thallophyta</subject><issn>1568-9883</issn><issn>1878-1470</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNp9kD1v2zAQhoWgBZKm-QHZuLRTpB5JySLRKQjyBRjJkHTIRJypo0NXplRSDuB_Xzo2ik6Z7u7Fc19vUZxzqDjw2Y9V9Yp9JQBUBaIC4EfFCVetKnndwqecNzNVaqXkcfElpRWA4ABwUtDTiJPHnmHo2ETrcYi56PwbxeSnLRscW3sbB7tNkw_pHftPKMc4dBvrw5ItKQzTdqTEfGCPgXyHbI6_6YI9vHwtPjvsE50d4mnx6-b6-equnD_e3l9dzksrGzWVqiVQtFjU2jqlnao1LlwNROBIOYlCgLYzyTvluGh0y0k3WUEpUc2Iozwtvu_n5rP-bChNZu2Tpb7HQMMmGa6bVsu2ziDfg_mTlCI5M0a_xrg1HMzOULMy2VCzM9SAMNnQ3PPtMByTxd5FDNanf40CaslbseN-7jnKn755iiZZT8FS5yPZyXSD_2DLXz1bjGE</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Hotto, Amber M.</creator><creator>Satchwell, Mike F.</creator><creator>Berry, Dianna L.</creator><creator>Gobler, Christopher J.</creator><creator>Boyer, Gregory L.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope><scope>F1W</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope></search><sort><creationdate>20080801</creationdate><title>Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY</title><author>Hotto, Amber M. ; Satchwell, Mike F. ; Berry, Dianna L. ; Gobler, Christopher J. ; Boyer, Gregory L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c358t-87e08ebb49cf89f849abf40ee0fe8f3a2209c631d8f125971e9509ca33a86e1a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Algae</topic><topic>Anabaena</topic><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Autoecology</topic><topic>Biological and medical sciences</topic><topic>Cyanobacteria</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>mcy genes</topic><topic>Microcystin</topic><topic>Microcystis</topic><topic>Oneida Lake</topic><topic>Planktothrix</topic><topic>Plant cytology, morphology, systematics, chorology and evolution</topic><topic>Plants and fungi</topic><topic>qPCR</topic><topic>Thallophyta</topic><toplevel>online_resources</toplevel><creatorcontrib>Hotto, Amber M.</creatorcontrib><creatorcontrib>Satchwell, Mike F.</creatorcontrib><creatorcontrib>Berry, Dianna L.</creatorcontrib><creatorcontrib>Gobler, Christopher J.</creatorcontrib><creatorcontrib>Boyer, Gregory L.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><jtitle>Harmful algae</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hotto, Amber M.</au><au>Satchwell, Mike F.</au><au>Berry, Dianna L.</au><au>Gobler, Christopher J.</au><au>Boyer, Gregory L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY</atitle><jtitle>Harmful algae</jtitle><date>2008-08-01</date><risdate>2008</risdate><volume>7</volume><issue>5</issue><spage>671</spage><epage>681</epage><pages>671-681</pages><issn>1568-9883</issn><eissn>1878-1470</eissn><abstract>Oneida Lake is a shallow, eutrophic lake with a well-established cyanobacterial population with reported toxic blooms containing hepatotoxic microcystins (MC). Peak bloom events from the summers of 2002 and 2003 were analyzed to determine the principal cyanobacterial genera containing microcystin synthetase (
mcy) genes. Sequence analysis of a partial
mcyA amplicon targeting
Microcystis,
Anabaena and
Planktothrix sp. indicated that
Microcystis sp. was the dominant
mcy genotype. This
Microcystis clade was split into two distinct sub-clades. Bloom events contained members of both sub-clades with the higher MC concentrations found when both sub-clades were present in near equal proportions. The proportion of
Microcystis containing the
mcyD gene ranged from 0 to 37% of the total
Microcystis population as determined by quantitative PCR (qPCR). The total concentration of
Microcystis containing
mcyD genes was linearly related to the concentration of MCs (
r
2
=
0.63). The relationship between
mcy genotype and physiochemical variables was examined to determine the factor(s) controlling the periodicity in MC production in Oneida Lake. Multivariate statistical analyses, used to correlate the continuous-response variables, revealed a strong relationship between chlorophyll
a, MCs and total
Microcystis.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.hal.2008.02.001</doi><tpages>11</tpages></addata></record> |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Algae Anabaena Animal and plant ecology Animal, plant and microbial ecology Autoecology Biological and medical sciences Cyanobacteria Freshwater Fundamental and applied biological sciences. Psychology mcy genes Microcystin Microcystis Oneida Lake Planktothrix Plant cytology, morphology, systematics, chorology and evolution Plants and fungi qPCR Thallophyta |
| title | Spatial and temporal diversity of microcystins and microcystin-producing genotypes in Oneida Lake, NY |
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