Ochratoxin A Determination in Beer by Solid-Phase Microextraction Coupled to Liquid Chromatography with Fluorescence Detection: A Fast and Sensitive Method for Assessment of Noncompliance to Legal Limits
A solid-phase microextraction-liquid chromatography-fluorescence detection (SPME−LC−FD) method for the determination of ochratoxin A (OTA) in commercial beer samples was developed for the first time using a 60 μm thick poly(dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber. The procedure required a...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2006-03, Vol.54 (5), p.1594-1598 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Aresta, Antonella Palmisano, Francesco Vatinno, Rosa Zambonin, Carlo G |
| description | A solid-phase microextraction-liquid chromatography-fluorescence detection (SPME−LC−FD) method for the determination of ochratoxin A (OTA) in commercial beer samples was developed for the first time using a 60 μm thick poly(dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber. The procedure required a very simple sample pretreatment, an isocratic elution, and provides a selective extraction. All of the factors influencing fiber adsorption (extraction time, temperature, pH, and salt addition) and desorption of the analyte (desorption and injection time and desorption solvent mixture composition) have been investigated. The linear range investigated in beer was 0.03−2 ng/mL; within-day and between-days relative standard deviation in beer were 4.3 and 5.9%, respectively. The limit of quantification in spiked beer was 53 pg mL-1, well below all European regulatory levels. |
| doi_str_mv | 10.1021/jf052666o |
| format | Article |
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The procedure required a very simple sample pretreatment, an isocratic elution, and provides a selective extraction. All of the factors influencing fiber adsorption (extraction time, temperature, pH, and salt addition) and desorption of the analyte (desorption and injection time and desorption solvent mixture composition) have been investigated. The linear range investigated in beer was 0.03−2 ng/mL; within-day and between-days relative standard deviation in beer were 4.3 and 5.9%, respectively. The limit of quantification in spiked beer was 53 pg mL-1, well below all European regulatory levels.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf052666o</identifier><identifier>PMID: 16506806</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Beer - analysis ; Beers ; Biological and medical sciences ; Chromatography, Liquid - methods ; detection ; Fermented food industries ; fluorescence ; food analysis ; food contamination ; Food industries ; Fundamental and applied biological sciences. 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Agric. Food Chem</addtitle><description>A solid-phase microextraction-liquid chromatography-fluorescence detection (SPME−LC−FD) method for the determination of ochratoxin A (OTA) in commercial beer samples was developed for the first time using a 60 μm thick poly(dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber. The procedure required a very simple sample pretreatment, an isocratic elution, and provides a selective extraction. All of the factors influencing fiber adsorption (extraction time, temperature, pH, and salt addition) and desorption of the analyte (desorption and injection time and desorption solvent mixture composition) have been investigated. The linear range investigated in beer was 0.03−2 ng/mL; within-day and between-days relative standard deviation in beer were 4.3 and 5.9%, respectively. The limit of quantification in spiked beer was 53 pg mL-1, well below all European regulatory levels.</description><subject>Beer - analysis</subject><subject>Beers</subject><subject>Biological and medical sciences</subject><subject>Chromatography, Liquid - methods</subject><subject>detection</subject><subject>Fermented food industries</subject><subject>fluorescence</subject><subject>food analysis</subject><subject>food contamination</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>liquid chromatography</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>microextraction</subject><subject>Mycotoxins - analysis</subject><subject>ochratoxin A</subject><subject>Ochratoxins - analysis</subject><subject>Quality Control</subject><subject>rapid methods</subject><subject>solid phase extraction</subject><subject>solid phase microextraction</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkc2O0zAUhS0EYkphwQuANyCxCNhObSfsSofOMCrMSJ1ZW25y07gkccd2oN2x5eV4CJ4E90fTDStLvt89R_cchF5S8p4SRj-sKsKZEMI-QgPKGUk4pdljNCBxmGRc0DP0zPsVISTjkjxFZ1RwIjIiBujPdVE7HezGdHiMzyGAa02ng7Edjl-fABxebPHcNqZMbmrtAX81hbOwCU4Xe2xi-3UDJQ4Wz8x9b0o8qZ1to-jS6XW9xT9NqPG06a0DX0BXwN5nv_zx76_f0XeqfcC6K_EcOm-C-RFdINS2xJV1eOw9eN9CF7Ct8DfbFbZdN0bvlHamsNRNtG5N8M_Rk0o3Hl4c3yG6m36-nVwms-uLL5PxLNGjVIQkzcUipwJYISrGWJ5nFZVMlsB4mrJUlnIktZQwkimTaclkLjWUealHrCpIWqRD9Pagu3b2vgcfVGvibU2jO7C9VzTnQvKoNkTvDmDMzHsHlVo702q3VZSoXXnqobzIvjqK9osWyhN5bCsCb46A9oVuKhcjMP7ESZ7njGaRSw6c8QE2D3PtvishU8nV7c1cza_O5eji6lLRyL8-8JW2Si9d1LybM0JTQonkUrCTsy68WtnedTHd_5zwD_ZLyZY</recordid><startdate>20060308</startdate><enddate>20060308</enddate><creator>Aresta, Antonella</creator><creator>Palmisano, Francesco</creator><creator>Vatinno, Rosa</creator><creator>Zambonin, Carlo G</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20060308</creationdate><title>Ochratoxin A Determination in Beer by Solid-Phase Microextraction Coupled to Liquid Chromatography with Fluorescence Detection: A Fast and Sensitive Method for Assessment of Noncompliance to Legal Limits</title><author>Aresta, Antonella ; Palmisano, Francesco ; Vatinno, Rosa ; Zambonin, Carlo G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a436t-396b916e2c6f222998f1727de2533237d747a77e473273d2797aed9da42fc03c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Beer - analysis</topic><topic>Beers</topic><topic>Biological and medical sciences</topic><topic>Chromatography, Liquid - methods</topic><topic>detection</topic><topic>Fermented food industries</topic><topic>fluorescence</topic><topic>food analysis</topic><topic>food contamination</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>liquid chromatography</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>microextraction</topic><topic>Mycotoxins - analysis</topic><topic>ochratoxin A</topic><topic>Ochratoxins - analysis</topic><topic>Quality Control</topic><topic>rapid methods</topic><topic>solid phase extraction</topic><topic>solid phase microextraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aresta, Antonella</creatorcontrib><creatorcontrib>Palmisano, Francesco</creatorcontrib><creatorcontrib>Vatinno, Rosa</creatorcontrib><creatorcontrib>Zambonin, Carlo G</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aresta, Antonella</au><au>Palmisano, Francesco</au><au>Vatinno, Rosa</au><au>Zambonin, Carlo G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ochratoxin A Determination in Beer by Solid-Phase Microextraction Coupled to Liquid Chromatography with Fluorescence Detection: A Fast and Sensitive Method for Assessment of Noncompliance to Legal Limits</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2006-03-08</date><risdate>2006</risdate><volume>54</volume><issue>5</issue><spage>1594</spage><epage>1598</epage><pages>1594-1598</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A solid-phase microextraction-liquid chromatography-fluorescence detection (SPME−LC−FD) method for the determination of ochratoxin A (OTA) in commercial beer samples was developed for the first time using a 60 μm thick poly(dimethylsiloxane)/divinylbenzene (PDMS/DVB) fiber. The procedure required a very simple sample pretreatment, an isocratic elution, and provides a selective extraction. All of the factors influencing fiber adsorption (extraction time, temperature, pH, and salt addition) and desorption of the analyte (desorption and injection time and desorption solvent mixture composition) have been investigated. 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| ispartof | Journal of agricultural and food chemistry, 2006-03, Vol.54 (5), p.1594-1598 |
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| language | eng |
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| source | MEDLINE; ACS_美国化学学会期刊（与NSTL共建） |
| subjects | Beer - analysis Beers Biological and medical sciences Chromatography, Liquid - methods detection Fermented food industries fluorescence food analysis food contamination Food industries Fundamental and applied biological sciences. Psychology General aspects liquid chromatography Methods of analysis, processing and quality control, regulation, standards microextraction Mycotoxins - analysis ochratoxin A Ochratoxins - analysis Quality Control rapid methods solid phase extraction solid phase microextraction |
| title | Ochratoxin A Determination in Beer by Solid-Phase Microextraction Coupled to Liquid Chromatography with Fluorescence Detection: A Fast and Sensitive Method for Assessment of Noncompliance to Legal Limits |
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