Co-encapsulation of gallium with gentamicin in liposomes enhances antimicrobial activity of gentamicin against Pseudomonas aeruginosa
Objectives The aim of this study was to enhance the antimicrobial efficacy of a liposomal gentamicin formulation with gallium metal (Lipo-Ga-GEN) against clinical isolates of Pseudomonas aeruginosa. Methods Sputum isolates of P. aeruginosa from cystic fibrosis patients were used to determine the MIC...
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| Veröffentlicht in: | Journal of antimicrobial chemotherapy 2008-12, Vol.62 (6), p.1291-1297 |
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| creator | Halwani, M. Yebio, B. Suntres, Z. E. Alipour, M. Azghani, A. O. Omri, A. |
| description | Objectives The aim of this study was to enhance the antimicrobial efficacy of a liposomal gentamicin formulation with gallium metal (Lipo-Ga-GEN) against clinical isolates of Pseudomonas aeruginosa. Methods Sputum isolates of P. aeruginosa from cystic fibrosis patients were used to determine the MIC and MBC of Lipo-Ga-GEN. P. aeruginosa biofilms were formed and used to compare the minimum biofilm eradication concentration of the conventional drugs with that of Lipo-Ga-GEN. Quorum sensing (QS) molecule reduction of P. aeruginosa was determined by monitoring N-acyl homoserine lactone production using Agrobacterium tumefaciens reporter strain (A136). Viability of the cultured human lung epithelial cells (A549) was determined by Trypan Blue assay in order to assess Ga toxicity. Results MIC and MBC values indicated that gentamicin was more effective against a highly resistant strain of P. aeruginosa (PA-48913) when delivered as a Lipo-Ga-GEN formulation (256 mg/L free gentamicin versus 2 mg/L Lipo-Ga-GEN). Lipo-Ga-GEN was the only formulation that completely eradicated biofilms and blocked QS molecules at a very low concentration (0.94 mg/L gentamicin). The decrease in cell viability was less in A549 cells exposed to Lipo-Ga, suggesting that encapsulated Ga is safer. Conclusions The results clearly indicate that the Lipo-Ga-GEN formulation is more effective than gentamicin alone in eradicating antibiotic-resistant P. aeruginosa isolates growing in a planktonic or biofilm community. |
| doi_str_mv | 10.1093/jac/dkn422 |
| format | Article |
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E. ; Alipour, M. ; Azghani, A. O. ; Omri, A.</creator><creatorcontrib>Halwani, M. ; Yebio, B. ; Suntres, Z. E. ; Alipour, M. ; Azghani, A. O. ; Omri, A.</creatorcontrib><description>Objectives The aim of this study was to enhance the antimicrobial efficacy of a liposomal gentamicin formulation with gallium metal (Lipo-Ga-GEN) against clinical isolates of Pseudomonas aeruginosa. Methods Sputum isolates of P. aeruginosa from cystic fibrosis patients were used to determine the MIC and MBC of Lipo-Ga-GEN. P. aeruginosa biofilms were formed and used to compare the minimum biofilm eradication concentration of the conventional drugs with that of Lipo-Ga-GEN. Quorum sensing (QS) molecule reduction of P. aeruginosa was determined by monitoring N-acyl homoserine lactone production using Agrobacterium tumefaciens reporter strain (A136). Viability of the cultured human lung epithelial cells (A549) was determined by Trypan Blue assay in order to assess Ga toxicity. Results MIC and MBC values indicated that gentamicin was more effective against a highly resistant strain of P. aeruginosa (PA-48913) when delivered as a Lipo-Ga-GEN formulation (256 mg/L free gentamicin versus 2 mg/L Lipo-Ga-GEN). Lipo-Ga-GEN was the only formulation that completely eradicated biofilms and blocked QS molecules at a very low concentration (0.94 mg/L gentamicin). The decrease in cell viability was less in A549 cells exposed to Lipo-Ga, suggesting that encapsulated Ga is safer. Conclusions The results clearly indicate that the Lipo-Ga-GEN formulation is more effective than gentamicin alone in eradicating antibiotic-resistant P. aeruginosa isolates growing in a planktonic or biofilm community.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkn422</identifier><identifier>PMID: 18931388</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Acyl-Butyrolactones - metabolism ; Agrobacterium tumefaciens ; Agrobacterium tumefaciens - growth & development ; aminoglycosides ; Anti-Bacterial Agents - metabolism ; Anti-Bacterial Agents - pharmacology ; antibiotics ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Biofilms ; Biofilms - drug effects ; Biological and medical sciences ; Cell Line ; Cell Survival ; Comparative studies ; Cystic fibrosis ; Cystic Fibrosis - complications ; Drug Synergism ; Epithelial Cells - microbiology ; Gallium - metabolism ; Gallium - pharmacology ; Gentamicins - metabolism ; Gentamicins - pharmacology ; Humans ; Liposomes - metabolism ; Medical sciences ; Microbial Sensitivity Tests ; Microbial Viability ; Microbiology ; Pharmacology ; Pharmacology. Drug treatments ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - drug effects ; Pseudomonas aeruginosa - isolation & purification ; Pseudomonas Infections - microbiology ; quorum sensing ; Sputum - microbiology ; toxicity ; Trypan Blue - metabolism</subject><ispartof>Journal of antimicrobial chemotherapy, 2008-12, Vol.62 (6), p.1291-1297</ispartof><rights>The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org 2008</rights><rights>2009 INIST-CNRS</rights><rights>The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c477t-ebd4cf2420806d44e85ed8ce8ddca0674fe78bff7083f9dcc4041f1b537577783</citedby><cites>FETCH-LOGICAL-c477t-ebd4cf2420806d44e85ed8ce8ddca0674fe78bff7083f9dcc4041f1b537577783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20923055$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18931388$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Halwani, M.</creatorcontrib><creatorcontrib>Yebio, B.</creatorcontrib><creatorcontrib>Suntres, Z. E.</creatorcontrib><creatorcontrib>Alipour, M.</creatorcontrib><creatorcontrib>Azghani, A. O.</creatorcontrib><creatorcontrib>Omri, A.</creatorcontrib><title>Co-encapsulation of gallium with gentamicin in liposomes enhances antimicrobial activity of gentamicin against Pseudomonas aeruginosa</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Objectives The aim of this study was to enhance the antimicrobial efficacy of a liposomal gentamicin formulation with gallium metal (Lipo-Ga-GEN) against clinical isolates of Pseudomonas aeruginosa. Methods Sputum isolates of P. aeruginosa from cystic fibrosis patients were used to determine the MIC and MBC of Lipo-Ga-GEN. P. aeruginosa biofilms were formed and used to compare the minimum biofilm eradication concentration of the conventional drugs with that of Lipo-Ga-GEN. Quorum sensing (QS) molecule reduction of P. aeruginosa was determined by monitoring N-acyl homoserine lactone production using Agrobacterium tumefaciens reporter strain (A136). Viability of the cultured human lung epithelial cells (A549) was determined by Trypan Blue assay in order to assess Ga toxicity. Results MIC and MBC values indicated that gentamicin was more effective against a highly resistant strain of P. aeruginosa (PA-48913) when delivered as a Lipo-Ga-GEN formulation (256 mg/L free gentamicin versus 2 mg/L Lipo-Ga-GEN). Lipo-Ga-GEN was the only formulation that completely eradicated biofilms and blocked QS molecules at a very low concentration (0.94 mg/L gentamicin). The decrease in cell viability was less in A549 cells exposed to Lipo-Ga, suggesting that encapsulated Ga is safer. Conclusions The results clearly indicate that the Lipo-Ga-GEN formulation is more effective than gentamicin alone in eradicating antibiotic-resistant P. aeruginosa isolates growing in a planktonic or biofilm community.</description><subject>Acyl-Butyrolactones - metabolism</subject><subject>Agrobacterium tumefaciens</subject><subject>Agrobacterium tumefaciens - growth & development</subject><subject>aminoglycosides</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotics</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell Survival</subject><subject>Comparative studies</subject><subject>Cystic fibrosis</subject><subject>Cystic Fibrosis - complications</subject><subject>Drug Synergism</subject><subject>Epithelial Cells - microbiology</subject><subject>Gallium - metabolism</subject><subject>Gallium - pharmacology</subject><subject>Gentamicins - metabolism</subject><subject>Gentamicins - pharmacology</subject><subject>Humans</subject><subject>Liposomes - metabolism</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Microbial Viability</subject><subject>Microbiology</subject><subject>Pharmacology</subject><subject>Pharmacology. Drug treatments</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - drug effects</subject><subject>Pseudomonas aeruginosa - isolation & purification</subject><subject>Pseudomonas Infections - microbiology</subject><subject>quorum sensing</subject><subject>Sputum - microbiology</subject><subject>toxicity</subject><subject>Trypan Blue - metabolism</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kd9qFDEUh4Modlu98QFkEOyFMDaZSSaZS1nUigUFq0hvwpn82WY7k4xJRu0D-N5Gd-mCF0IggXznd5LzIfSE4JcE9-3ZFtSZvvG0ae6hFaEdrhvck_tohVvMak5Ze4SOU9pijDvWiYfoiIi-Ja0QK_RrHWrjFcxpGSG74Ktgqw2Mo1um6ofL19XG-AyTU85XZY1uDilMJlXGX4NX5QA-u3Ifw-BgrEBl993l2785h1LYgPMpVx-TWXSYgodSaOKycT4keIQeWBiTebzfT9DnN68v1-f1xYe379avLmpFOc-1GTRVtqENFrjTlBrBjBbKCK0V4I5Ta7gYrOVYtLbXSlFMiSUDaznjnIv2BJ3ucucYvi0mZTm5pMw4gjdhSZL0rKMNbwr47B9wG5boy9tkQ3jXsZ7gAr3YQeXvKUVj5RzdBPFWEiz_mJHFjNyZKfDTfeIyTEYf0L2KAjzfA5AUjDaW6bp0xxWlTdHJDlxY5v83rHecS9n8vCMh3siOl4HI869XEl9R9umSf5Hv29_2y7YQ</recordid><startdate>20081201</startdate><enddate>20081201</enddate><creator>Halwani, M.</creator><creator>Yebio, B.</creator><creator>Suntres, Z. E.</creator><creator>Alipour, M.</creator><creator>Azghani, A. O.</creator><creator>Omri, A.</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope></search><sort><creationdate>20081201</creationdate><title>Co-encapsulation of gallium with gentamicin in liposomes enhances antimicrobial activity of gentamicin against Pseudomonas aeruginosa</title><author>Halwani, M. ; Yebio, B. ; Suntres, Z. E. ; Alipour, M. ; Azghani, A. O. ; Omri, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c477t-ebd4cf2420806d44e85ed8ce8ddca0674fe78bff7083f9dcc4041f1b537577783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acyl-Butyrolactones - metabolism</topic><topic>Agrobacterium tumefaciens</topic><topic>Agrobacterium tumefaciens - growth & development</topic><topic>aminoglycosides</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiotics</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell Survival</topic><topic>Comparative studies</topic><topic>Cystic fibrosis</topic><topic>Cystic Fibrosis - complications</topic><topic>Drug Synergism</topic><topic>Epithelial Cells - microbiology</topic><topic>Gallium - metabolism</topic><topic>Gallium - pharmacology</topic><topic>Gentamicins - metabolism</topic><topic>Gentamicins - pharmacology</topic><topic>Humans</topic><topic>Liposomes - metabolism</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Microbial Viability</topic><topic>Microbiology</topic><topic>Pharmacology</topic><topic>Pharmacology. Drug treatments</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - drug effects</topic><topic>Pseudomonas aeruginosa - isolation & purification</topic><topic>Pseudomonas Infections - microbiology</topic><topic>quorum sensing</topic><topic>Sputum - microbiology</topic><topic>toxicity</topic><topic>Trypan Blue - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Halwani, M.</creatorcontrib><creatorcontrib>Yebio, B.</creatorcontrib><creatorcontrib>Suntres, Z. E.</creatorcontrib><creatorcontrib>Alipour, M.</creatorcontrib><creatorcontrib>Azghani, A. O.</creatorcontrib><creatorcontrib>Omri, A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Halwani, M.</au><au>Yebio, B.</au><au>Suntres, Z. E.</au><au>Alipour, M.</au><au>Azghani, A. O.</au><au>Omri, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co-encapsulation of gallium with gentamicin in liposomes enhances antimicrobial activity of gentamicin against Pseudomonas aeruginosa</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2008-12-01</date><risdate>2008</risdate><volume>62</volume><issue>6</issue><spage>1291</spage><epage>1297</epage><pages>1291-1297</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives The aim of this study was to enhance the antimicrobial efficacy of a liposomal gentamicin formulation with gallium metal (Lipo-Ga-GEN) against clinical isolates of Pseudomonas aeruginosa. Methods Sputum isolates of P. aeruginosa from cystic fibrosis patients were used to determine the MIC and MBC of Lipo-Ga-GEN. P. aeruginosa biofilms were formed and used to compare the minimum biofilm eradication concentration of the conventional drugs with that of Lipo-Ga-GEN. Quorum sensing (QS) molecule reduction of P. aeruginosa was determined by monitoring N-acyl homoserine lactone production using Agrobacterium tumefaciens reporter strain (A136). Viability of the cultured human lung epithelial cells (A549) was determined by Trypan Blue assay in order to assess Ga toxicity. Results MIC and MBC values indicated that gentamicin was more effective against a highly resistant strain of P. aeruginosa (PA-48913) when delivered as a Lipo-Ga-GEN formulation (256 mg/L free gentamicin versus 2 mg/L Lipo-Ga-GEN). Lipo-Ga-GEN was the only formulation that completely eradicated biofilms and blocked QS molecules at a very low concentration (0.94 mg/L gentamicin). The decrease in cell viability was less in A549 cells exposed to Lipo-Ga, suggesting that encapsulated Ga is safer. Conclusions The results clearly indicate that the Lipo-Ga-GEN formulation is more effective than gentamicin alone in eradicating antibiotic-resistant P. aeruginosa isolates growing in a planktonic or biofilm community.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>18931388</pmid><doi>10.1093/jac/dkn422</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acyl-Butyrolactones - metabolism Agrobacterium tumefaciens Agrobacterium tumefaciens - growth & development aminoglycosides Anti-Bacterial Agents - metabolism Anti-Bacterial Agents - pharmacology antibiotics Antibiotics. Antiinfectious agents. Antiparasitic agents Biofilms Biofilms - drug effects Biological and medical sciences Cell Line Cell Survival Comparative studies Cystic fibrosis Cystic Fibrosis - complications Drug Synergism Epithelial Cells - microbiology Gallium - metabolism Gallium - pharmacology Gentamicins - metabolism Gentamicins - pharmacology Humans Liposomes - metabolism Medical sciences Microbial Sensitivity Tests Microbial Viability Microbiology Pharmacology Pharmacology. Drug treatments Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - isolation & purification Pseudomonas Infections - microbiology quorum sensing Sputum - microbiology toxicity Trypan Blue - metabolism |
| title | Co-encapsulation of gallium with gentamicin in liposomes enhances antimicrobial activity of gentamicin against Pseudomonas aeruginosa |
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