XdhR negatively regulates actinorhodin biosynthesis in Streptomyces coelicolor M145
Abstract The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overpr...
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| Veröffentlicht in: | FEMS microbiology letters 2017-12, Vol.364 (22) |
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| container_title | FEMS microbiology letters |
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| creator | Fu, Jiafang Zong, Gongli Zhang, Peipei Zhao, Zhilong Ma, Junxia Pang, Xiuhua Cao, Guangxiang |
| description | Abstract
The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overproduction of actinorhodin by approximately 2.5-fold compared to the wild-type strain. Complementation of the xdhR deletion strain restored actinorhodin production to normal levels. In addition, the relative expression levels of actinorhodin cluster genes were all significantly increased in the xdhR deletion strain compared to the wild-type strain. XdhR can specifically bind the promoters of actII-4 and actII-1, two pathway-specific regulators of actinorhodin biosynthesis. These results suggest that xdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in S. coelicolor.
XdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in Streptomyces coelicolor. |
| doi_str_mv | 10.1093/femsle/fnx226 |
| format | Article |
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The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overproduction of actinorhodin by approximately 2.5-fold compared to the wild-type strain. Complementation of the xdhR deletion strain restored actinorhodin production to normal levels. In addition, the relative expression levels of actinorhodin cluster genes were all significantly increased in the xdhR deletion strain compared to the wild-type strain. XdhR can specifically bind the promoters of actII-4 and actII-1, two pathway-specific regulators of actinorhodin biosynthesis. These results suggest that xdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in S. coelicolor.
XdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in Streptomyces coelicolor.</description><identifier>ISSN: 1574-6968</identifier><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1093/femsle/fnx226</identifier><identifier>PMID: 29069461</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Actinorhodin ; Anthraquinones - analysis ; Anthraquinones - metabolism ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biosynthesis ; Complementation ; Escherichia coli - genetics ; Gene Deletion ; Gene expression ; Gene Expression Regulation, Bacterial - genetics ; Genome, Bacterial ; Microbiology ; Promoter Regions, Genetic ; Protein Binding ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Regulators ; Streptomyces coelicolor ; Streptomyces coelicolor - genetics ; Streptomyces coelicolor - metabolism ; Xanthine Dehydrogenase - genetics ; Xanthine Dehydrogenase - metabolism</subject><ispartof>FEMS microbiology letters, 2017-12, Vol.364 (22)</ispartof><rights>FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2017</rights><rights>FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>Copyright Oxford University Press, UK Dec 2017</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-b7bd95624e81632e5b77f9eb14063bda65f53a2762bd1f0cf1f30798e59c54953</citedby><cites>FETCH-LOGICAL-c393t-b7bd95624e81632e5b77f9eb14063bda65f53a2762bd1f0cf1f30798e59c54953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1584,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29069461$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Jiafang</creatorcontrib><creatorcontrib>Zong, Gongli</creatorcontrib><creatorcontrib>Zhang, Peipei</creatorcontrib><creatorcontrib>Zhao, Zhilong</creatorcontrib><creatorcontrib>Ma, Junxia</creatorcontrib><creatorcontrib>Pang, Xiuhua</creatorcontrib><creatorcontrib>Cao, Guangxiang</creatorcontrib><title>XdhR negatively regulates actinorhodin biosynthesis in Streptomyces coelicolor M145</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overproduction of actinorhodin by approximately 2.5-fold compared to the wild-type strain. Complementation of the xdhR deletion strain restored actinorhodin production to normal levels. In addition, the relative expression levels of actinorhodin cluster genes were all significantly increased in the xdhR deletion strain compared to the wild-type strain. XdhR can specifically bind the promoters of actII-4 and actII-1, two pathway-specific regulators of actinorhodin biosynthesis. These results suggest that xdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in S. coelicolor.
XdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in Streptomyces coelicolor.</description><subject>Actinorhodin</subject><subject>Anthraquinones - analysis</subject><subject>Anthraquinones - metabolism</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biosynthesis</subject><subject>Complementation</subject><subject>Escherichia coli - genetics</subject><subject>Gene Deletion</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial - genetics</subject><subject>Genome, Bacterial</subject><subject>Microbiology</subject><subject>Promoter Regions, Genetic</subject><subject>Protein Binding</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Regulators</subject><subject>Streptomyces coelicolor</subject><subject>Streptomyces coelicolor - genetics</subject><subject>Streptomyces coelicolor - metabolism</subject><subject>Xanthine Dehydrogenase - genetics</subject><subject>Xanthine Dehydrogenase - metabolism</subject><issn>1574-6968</issn><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkEtLxDAQgIMo7rp69CoFL16qeTRpc5TFF6wIroK30qaT3S5tU5NU7L-30vWBF08zAx8fw4fQMcHnBEt2oaF2FVzo5p1SsYOmhMdRKKRIdn_tE3Tg3AZjHFEs9tGESixkJMgULV-K9WPQwCrz5RtUfWBh1VWZBxdkypeNsWtTlE2Ql8b1jV-DK10w3EtvofWm7tVAKgNVqUxlbHBPIn6I9nRWOTjazhl6vr56mt-Gi4ebu_nlIlRMMh_mcV5ILmgECRGMAs_jWEvISYQFy4tMcM1ZRmNB84JorDTRDMcyAS4VjyRnM3Q2eltrXjtwPq1Lp6CqsgZM51Iy2LFMcEwH9PQPujGdbYbv0qFILImULBmocKSUNc5Z0GlryzqzfUpw-lk7HWunY-2BP9lau7yG4pv-yvvzoenaf1wfBkOKuw</recordid><startdate>20171201</startdate><enddate>20171201</enddate><creator>Fu, Jiafang</creator><creator>Zong, Gongli</creator><creator>Zhang, Peipei</creator><creator>Zhao, Zhilong</creator><creator>Ma, Junxia</creator><creator>Pang, Xiuhua</creator><creator>Cao, Guangxiang</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20171201</creationdate><title>XdhR negatively regulates actinorhodin biosynthesis in Streptomyces coelicolor M145</title><author>Fu, Jiafang ; Zong, Gongli ; Zhang, Peipei ; Zhao, Zhilong ; Ma, Junxia ; Pang, Xiuhua ; Cao, Guangxiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-b7bd95624e81632e5b77f9eb14063bda65f53a2762bd1f0cf1f30798e59c54953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Actinorhodin</topic><topic>Anthraquinones - analysis</topic><topic>Anthraquinones - metabolism</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biosynthesis</topic><topic>Complementation</topic><topic>Escherichia coli - genetics</topic><topic>Gene Deletion</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial - genetics</topic><topic>Genome, Bacterial</topic><topic>Microbiology</topic><topic>Promoter Regions, Genetic</topic><topic>Protein Binding</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Regulators</topic><topic>Streptomyces coelicolor</topic><topic>Streptomyces coelicolor - genetics</topic><topic>Streptomyces coelicolor - metabolism</topic><topic>Xanthine Dehydrogenase - genetics</topic><topic>Xanthine Dehydrogenase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Jiafang</creatorcontrib><creatorcontrib>Zong, Gongli</creatorcontrib><creatorcontrib>Zhang, Peipei</creatorcontrib><creatorcontrib>Zhao, Zhilong</creatorcontrib><creatorcontrib>Ma, Junxia</creatorcontrib><creatorcontrib>Pang, Xiuhua</creatorcontrib><creatorcontrib>Cao, Guangxiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Jiafang</au><au>Zong, Gongli</au><au>Zhang, Peipei</au><au>Zhao, Zhilong</au><au>Ma, Junxia</au><au>Pang, Xiuhua</au><au>Cao, Guangxiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>XdhR negatively regulates actinorhodin biosynthesis in Streptomyces coelicolor M145</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2017-12-01</date><risdate>2017</risdate><volume>364</volume><issue>22</issue><issn>1574-6968</issn><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
The xdhR gene encodes a TetR-family regulator in Streptomyces coelicolor. However, little is known about the function of XdhR in regulating actinorhodin production. Here, we report that XdhR negatively regulates actinorhodin biosynthesis in S. coelicolor. Deletion of xdhR resulted in overproduction of actinorhodin by approximately 2.5-fold compared to the wild-type strain. Complementation of the xdhR deletion strain restored actinorhodin production to normal levels. In addition, the relative expression levels of actinorhodin cluster genes were all significantly increased in the xdhR deletion strain compared to the wild-type strain. XdhR can specifically bind the promoters of actII-4 and actII-1, two pathway-specific regulators of actinorhodin biosynthesis. These results suggest that xdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in S. coelicolor.
XdhR negatively controls actinorhodin biosynthesis by directly regulating actII-4 and actII-1 in Streptomyces coelicolor.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>29069461</pmid><doi>10.1093/femsle/fnx226</doi><oa>free_for_read</oa></addata></record> |
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| ispartof | FEMS microbiology letters, 2017-12, Vol.364 (22) |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Actinorhodin Anthraquinones - analysis Anthraquinones - metabolism Bacterial Proteins - genetics Bacterial Proteins - metabolism Biosynthesis Complementation Escherichia coli - genetics Gene Deletion Gene expression Gene Expression Regulation, Bacterial - genetics Genome, Bacterial Microbiology Promoter Regions, Genetic Protein Binding Recombinant Proteins - genetics Recombinant Proteins - metabolism Regulators Streptomyces coelicolor Streptomyces coelicolor - genetics Streptomyces coelicolor - metabolism Xanthine Dehydrogenase - genetics Xanthine Dehydrogenase - metabolism |
| title | XdhR negatively regulates actinorhodin biosynthesis in Streptomyces coelicolor M145 |
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