Portable GMR Handheld Platform for the Detection of Influenza A Virus
Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human...
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| Veröffentlicht in: | ACS sensors 2017-11, Vol.2 (11), p.1594-1601 |
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| creator | Wu, Kai Klein, Todd Krishna, Venkatramana D. Su, Diqing Perez, Andres M. Wang, Jian-Ping |
| description | Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings. |
| doi_str_mv | 10.1021/acssensors.7b00432 |
| format | Article |
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Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.</description><identifier>ISSN: 2379-3694</identifier><identifier>EISSN: 2379-3694</identifier><identifier>DOI: 10.1021/acssensors.7b00432</identifier><identifier>PMID: 29068663</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Immunoassay - instrumentation ; Influenza A Virus, H3N2 Subtype - isolation & purification ; Magnetic Phenomena ; Nanotechnology - instrumentation ; Nucleoproteins - analysis ; Point-of-Care Systems</subject><ispartof>ACS sensors, 2017-11, Vol.2 (11), p.1594-1601</ispartof><rights>Copyright © 2017 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a342t-bc4af5980198c8ffe5b705c6532bd2dd154ca7b4f93a86de2c7d10d61e35fabb3</citedby><cites>FETCH-LOGICAL-a342t-bc4af5980198c8ffe5b705c6532bd2dd154ca7b4f93a86de2c7d10d61e35fabb3</cites><orcidid>0000-0002-9444-6112</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acssensors.7b00432$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acssensors.7b00432$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2765,27076,27924,27925,56738,56788</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29068663$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Kai</creatorcontrib><creatorcontrib>Klein, Todd</creatorcontrib><creatorcontrib>Krishna, Venkatramana D.</creatorcontrib><creatorcontrib>Su, Diqing</creatorcontrib><creatorcontrib>Perez, Andres M.</creatorcontrib><creatorcontrib>Wang, Jian-Ping</creatorcontrib><title>Portable GMR Handheld Platform for the Detection of Influenza A Virus</title><title>ACS sensors</title><addtitle>ACS Sens</addtitle><description>Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). 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This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.</description><subject>Immunoassay - instrumentation</subject><subject>Influenza A Virus, H3N2 Subtype - isolation & purification</subject><subject>Magnetic Phenomena</subject><subject>Nanotechnology - instrumentation</subject><subject>Nucleoproteins - analysis</subject><subject>Point-of-Care Systems</subject><issn>2379-3694</issn><issn>2379-3694</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kLtOwzAUhi0EolXpCzAgjywtvsSOM1altJVAVAhYLV_VVElc7GSApyeo5TKxnHOG7_-l8wFwidEUI4JvlEnJNSnENM01QhklJ2BIaF5MKC-y0z_3AIxT2iGEMOOECXQOBqRAXHBOh2CxCbFVunJw-fAEV6qxW1dZuKlU60OsYT9gu3Xw1rXOtGVoYPBw3fiqc82HgjP4WsYuXYAzr6rkxsc9Ai93i-f5anL_uFzPZ_cTRTPSTrTJlGeFQLgQRnjvmM4RM5xRoi2xFrPMqFxnvqBKcOuIyS1GlmNHmVda0xG4PvTuY3jrXGplXSbjqko1LnRJ4oJxJATLsh4lB9TEkFJ0Xu5jWav4LjGSXwblr0F5NNiHro79na6d_Yl8--qB6QHow3IXutj07_7X-AkA936J</recordid><startdate>20171122</startdate><enddate>20171122</enddate><creator>Wu, Kai</creator><creator>Klein, Todd</creator><creator>Krishna, Venkatramana D.</creator><creator>Su, Diqing</creator><creator>Perez, Andres M.</creator><creator>Wang, Jian-Ping</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9444-6112</orcidid></search><sort><creationdate>20171122</creationdate><title>Portable GMR Handheld Platform for the Detection of Influenza A Virus</title><author>Wu, Kai ; Klein, Todd ; Krishna, Venkatramana D. ; Su, Diqing ; Perez, Andres M. ; Wang, Jian-Ping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a342t-bc4af5980198c8ffe5b705c6532bd2dd154ca7b4f93a86de2c7d10d61e35fabb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Immunoassay - instrumentation</topic><topic>Influenza A Virus, H3N2 Subtype - isolation & purification</topic><topic>Magnetic Phenomena</topic><topic>Nanotechnology - instrumentation</topic><topic>Nucleoproteins - analysis</topic><topic>Point-of-Care Systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Kai</creatorcontrib><creatorcontrib>Klein, Todd</creatorcontrib><creatorcontrib>Krishna, Venkatramana D.</creatorcontrib><creatorcontrib>Su, Diqing</creatorcontrib><creatorcontrib>Perez, Andres M.</creatorcontrib><creatorcontrib>Wang, Jian-Ping</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS sensors</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Kai</au><au>Klein, Todd</au><au>Krishna, Venkatramana D.</au><au>Su, Diqing</au><au>Perez, Andres M.</au><au>Wang, Jian-Ping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Portable GMR Handheld Platform for the Detection of Influenza A Virus</atitle><jtitle>ACS sensors</jtitle><addtitle>ACS Sens</addtitle><date>2017-11-22</date><risdate>2017</risdate><volume>2</volume><issue>11</issue><spage>1594</spage><epage>1601</epage><pages>1594-1601</pages><issn>2379-3694</issn><eissn>2379-3694</eissn><abstract>Influenza A virus (IAV) is a common respiratory pathogen infecting many hosts including humans, pigs (swine influenza virus or SIV), and birds (avian influenza virus or AIV). Monitoring swine and avian influenza viruses in the wild, farms, and live poultry markets is of great significance for human and veterinary public health. A portable, sensitive, and quantitative immunoassay device will be of high demand especially in the rural and resource-limited areas. We report herein our Z-Lab point-of-care (POC) device for sensitive and specific detection of swine influenza viruses with minimum sample handling and laboratory skill requirements. In the present study, a portable and quantitative immunoassay platform based on giant magnetoresistive (GMR) technology is used for the detection of IAV nucleoprotein (NP) and purified H3N2v. Z-Lab displays quantitative results in less than 10 min with sensitivities down to 15 ng/mL and 125 TCID50/mL for IAV nucleoprotein and purified H3N2v, respectively. This platform allows lab-testing to be performed outdoors and opens up the applications of immunoassays in nonclinical settings.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>29068663</pmid><doi>10.1021/acssensors.7b00432</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0002-9444-6112</orcidid></addata></record> |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Immunoassay - instrumentation Influenza A Virus, H3N2 Subtype - isolation & purification Magnetic Phenomena Nanotechnology - instrumentation Nucleoproteins - analysis Point-of-Care Systems |
| title | Portable GMR Handheld Platform for the Detection of Influenza A Virus |
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